{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T22:57:59Z","timestamp":1776293879814,"version":"3.50.1"},"reference-count":32,"publisher":"Springer Science and Business Media LLC","issue":"6","license":[{"start":{"date-parts":[[2021,8,7]],"date-time":"2021-08-07T00:00:00Z","timestamp":1628294400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2021,8,7]],"date-time":"2021-08-07T00:00:00Z","timestamp":1628294400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Military Amputee Research Program at Walter Reed Army Medical Center","award":["P05-A011"],"award-info":[{"award-number":["P05-A011"]}]},{"name":"Comprehensive Neurosciences Program","award":["CNP-2008-CR01"],"award-info":[{"award-number":["CNP-2008-CR01"]}]},{"name":"Defense Medical Research and Development Program","award":["D10_1_AR_J5_920"],"award-info":[{"award-number":["D10_1_AR_J5_920"]}]},{"DOI":"10.13039\/100000090","name":"Congressionally Directed Medical Research Programs","doi-asserted-by":"crossref","award":["W81XWH-13-2-0083"],"award-info":[{"award-number":["W81XWH-13-2-0083"]}],"id":[{"id":"10.13039\/100000090","id-type":"DOI","asserted-by":"crossref"}]},{"name":"NIH Intramural Research Program","award":["Z01 AR41131"],"award-info":[{"award-number":["Z01 AR41131"]}]},{"name":"USAMRAA BAA","award":["W81XWH-17-2-0009"],"award-info":[{"award-number":["W81XWH-17-2-0009"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Tissue Eng Regen Med"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>BACKGROUND:<\/jats:title>\n                <jats:p>The development of post-traumatic heterotopic ossification (HO) is a common, undesirable sequela in patients with high-energy (war-related) extremity injuries. While inflammatory and osteoinductive signaling pathways are known to be involved in the development and progression of post-traumatic HO, features of the structural microenvironment within which the ectopic bone begins to form remain poorly understood. Thus, increasing our knowledge of molecular and structural changes within the healing wound may help elucidate the pathogenesis of post-traumatic HO and aid in the development of specific treatment and\/or prevention strategies.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>METHODS:<\/jats:title>\n                <jats:p>In this study, we performed high-resolution microscopy and biochemical analysis of tissues obtained from traumatic war wounds to characterize changes in the structural microenvironment. In addition, using an electrospinning approach, we modeled this microenvironment to reconstitute a three-dimensional type I collagen scaffold with non-woven, randomly oriented nanofibers where we evaluated the performance of primary mesenchymal progenitor cells.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>RESULTS:<\/jats:title>\n                <jats:p>We found that traumatic war wounds are characterized by a disorganized, densely fibrotic collagen I matrix that influences progenitor cells adhesion, proliferation and osteogenic differentiation potential.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>CONCLUSION:<\/jats:title>\n                <jats:p>Altogether, these results suggest that the structural microenvironment present in traumatic war wounds has the potential to contribute to the development of post-traumatic HO. Our findings may support novel treatment strategies directed towards modifying the structural microenvironment after traumatic injury.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1007\/s13770-021-00355-y","type":"journal-article","created":{"date-parts":[[2021,8,7]],"date-time":"2021-08-07T15:07:15Z","timestamp":1628348835000},"page":"963-973","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Three-Dimensional Modeling of the Structural Microenvironment in Post-Traumatic War Wounds"],"prefix":"10.1007","volume":"18","author":[{"given":"Gregory T.","family":"Christopherson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaira F.","family":"de Vasconcellos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John C.","family":"Dunn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel W.","family":"Griffin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick E.","family":"Jones","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leon J.","family":"Nesti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,8,7]]},"reference":[{"key":"355_CR1","doi-asserted-by":"publisher","first-page":"2390","DOI":"10.2106\/JBJS.H.00049","volume":"90","author":"LJ Nesti","year":"2008","unstructured":"Nesti LJ, Jackson WM, Shanti RM, Koehler SM, Aragon AB, Bailey JR, et al. 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Uninjured, control muscle samples, were obtained from hamstring harvest of patients undergoing ligamentous knee reconstruction surgery at the Walter Reed National Military Medical Center. The Walter Reed National Military Medical Center Institutional Review Board approved this tissue procurement protocol. The amount of tissue debrided was determined by the operative surgeon. After surgical debridement of the tissue, de-identified samples were placed in a sterile container and transported on ice to the laboratory for processing. All samples were divided for (i) snap freezing in liquid nitrogen, (ii) fixation for scanning electron microscopy evaluation and (iii) isolation of primary mesenchymal progenitor cells (MPCs).","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical statement"}},{"value":"The contents of this publication are the sole responsibility of the author(s) and do not necessarily reflect the views, opinions or policies of Uniformed Services University of the Health Sciences (USUHS), The Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc.,\u00a0the Department of Defense (DoD), the Departments of the Army, Navy, or Air Force. Mention of trade names, commercial products, or organizations does not imply endorsement by the U.S. Government.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Disclaimer"}}]}}