{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T19:40:25Z","timestamp":1785526825775,"version":"3.56.0"},"reference-count":34,"publisher":"Springer Science and Business Media LLC","issue":"6","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82301037"],"award-info":[{"award-number":["82301037"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Tissue Eng Regen Med"],"published-print":{"date-parts":[[2026,8]]},"DOI":"10.1007\/s13770-026-00815-3","type":"journal-article","created":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T17:59:13Z","timestamp":1782928753000},"page":"851-865","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["In-Depth Analysis of AG73-Dental Pulp Cell Interactions Underlying Adhesion and Mineralization: Insights from Alanine Scanning"],"prefix":"10.1007","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5025-2674","authenticated-orcid":false,"given":"Jia","family":"Tang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akimi","family":"Muramatsu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youjing","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,7,1]]},"reference":[{"key":"815_CR1","doi-asserted-by":"publisher","first-page":"949","DOI":"10.1111\/iej.13128","volume":"52","author":"L Bj\u00f8rndal","year":"2019","unstructured":"Bj\u00f8rndal L, Simon S, Tomson PL, Duncan HF. Management of deep caries and the exposed pulp. Int Endod J. 2019;52:949\u201373.","journal-title":"Int Endod J"},{"key":"815_CR2","doi-asserted-by":"publisher","first-page":"489","DOI":"10.1186\/s12903-025-05872-z","volume":"25","author":"T Tasdemir","year":"2025","unstructured":"Tasdemir T, Eymirli PS, Ileri T, Ozler CO, Turgut MD. Management of crown fractures with different pulp treatments and Re-attachment technique: case series. BMC Oral Health. 2025;25:489.","journal-title":"BMC Oral Health"},{"key":"815_CR3","first-page":"17055","volume":"8","author":"C Zhu","year":"2015","unstructured":"Zhu C, Ju B, Ni R. Clinical outcome of direct pulp capping with MTA or calcium hydroxide: a systematic review and meta-analysis. Int J Clin Exp Med. 2015;8:17055\u201360.","journal-title":"Int J Clin Exp Med"},{"key":"815_CR4","doi-asserted-by":"publisher","first-page":"541","DOI":"10.1177\/002203458506400407","volume":"64","author":"U Schr\u00f6der","year":"1985","unstructured":"Schr\u00f6der U. Effects of calcium hydroxide-containing pulp-capping agents on pulp cell migration, proliferation, and differentiation. J Dent Res. 1985;64:541\u20138.","journal-title":"J Dent Res"},{"key":"815_CR5","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1016\/j.jobcr.2024.12.010","volume":"15","author":"S Sikaria","year":"2025","unstructured":"Sikaria S, Selvam SP, Ramadoss R, Sundar S. Membrana preformativa: unveiling the unexplored facets of dental development. J Oral Biol Craniofacial Res. 2025;15:84\u20137.","journal-title":"J Oral Biol Craniofacial Res"},{"key":"815_CR6","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1016\/j.matbio.2025.02.005","volume":"137","author":"A Nasrollahi","year":"2025","unstructured":"Nasrollahi A, Yao Y. Laminins and the blood-brain barrier. Matrix Biol. 2025;137:33\u201341.","journal-title":"Matrix Biol"},{"key":"815_CR7","doi-asserted-by":"publisher","first-page":"253","DOI":"10.2334\/josnusd.17-0286","volume":"60","author":"J Tang","year":"2018","unstructured":"Tang J, Saito T. Laminin-1 acts as an adhesive for odontoblast-like cells and promotes their differentiation toward a hard tissue-forming phenotype. J Oral Sci. 2018;60:253\u201361.","journal-title":"J Oral Sci"},{"key":"815_CR8","first-page":"1267","volume":"136","author":"LW Heck","year":"1990","unstructured":"Heck LW, Blackburn WD, Irwin MH, Abrahamson DR. Degradation of basement membrane laminin by human neutrophil elastase and cathepsin G. Am J Pathol. 1990;136:1267\u201374.","journal-title":"Am J Pathol"},{"key":"815_CR9","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1016\/j.abb.2010.08.012","volume":"503","author":"K Hozumi","year":"2010","unstructured":"Hozumi K, Akizuki T, Yamada Y, Hara T, Urushibata S, Katagiri F, et al. Cell adhesive peptide screening of the mouse laminin \u03b11 chain G domain. Arch Biochem Biophys. 2010;503:213\u201322.","journal-title":"Arch Biochem Biophys"},{"key":"815_CR10","doi-asserted-by":"publisher","first-page":"16174","DOI":"10.1016\/S0021-9258(18)71604-9","volume":"264","author":"KI Tashiro","year":"1989","unstructured":"Tashiro KI, Sephel GC, Weeks B, Sasaki M, Martin GR, Kleinman HK, et al. A synthetic peptide containing the IKVAV sequence from the a chain of laminin mediates cell attachment, migration, and neurite outgrowth. J Biol Chem. 1989;264:16174\u201382.","journal-title":"J Biol Chem"},{"key":"815_CR11","doi-asserted-by":"publisher","first-page":"1898","DOI":"10.1038\/sj.bjc.6690618","volume":"80","author":"N Yoshida","year":"1999","unstructured":"Yoshida N, Ishii E, Nomizu M, Yamada Y, Mohri S, Kinukawa N, et al. The laminin-derived peptide YIGSR (Tyr-Ile-Gly-Ser-Arg) inhibits human pre-B leukaemic cell growth and dissemination to organs in SCID mice. Br J Cancer. 1999;80:1898\u2013904.","journal-title":"Br J Cancer"},{"key":"815_CR12","doi-asserted-by":"publisher","first-page":"170","DOI":"10.1186\/1465-9921-11-170","volume":"11","author":"BGJ Dekkers","year":"2010","unstructured":"Dekkers BGJ, Bos IST, Halayko AJ, Zaagsma J, Meurs H. The laminin \u03b21-competing peptide YIGSR induces a hypercontractile, hypoproliferative airway smooth muscle phenotype in an animal model of allergic asthma. Respir Res. 2010;11:170.","journal-title":"Respir Res"},{"key":"815_CR13","doi-asserted-by":"publisher","first-page":"249","DOI":"10.1016\/j.abb.2006.12.026","volume":"459","author":"M Mochizuki","year":"2007","unstructured":"Mochizuki M, Philp D, Hozumi K, Suzuki N, Yamada Y, Kleinman HK, et al. Angiogenic activitiy of syndecan-binding laminin peptide AG73 (RKRLQVQLSIRT). Arch Biochem Biophys. 2007;459:249\u201355.","journal-title":"Arch Biochem Biophys"},{"key":"815_CR14","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1021\/mp900214s","volume":"7","author":"Y Negishi","year":"2010","unstructured":"Negishi Y, Omata D, Iijima H, Takabayashi Y, Suzuki K, Endo Y, et al. Enhanced laminin-derived peptide AG73-mediated liposomal gene transfer by bubble liposomes and ultrasound. Mol Pharm. 2010;7:217\u201326.","journal-title":"Mol Pharm"},{"key":"815_CR15","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2021.121113","volume":"277","author":"X Tan","year":"2021","unstructured":"Tan X, Jain E, Barcellona MN, Morris E, Neal S, Gupta MC, et al. Integrin and syndecan binding peptide-conjugated alginate hydrogel for modulation of nucleus pulposus cell phenotype. Biomaterials. 2021;277:121113.","journal-title":"Biomaterials"},{"key":"815_CR16","doi-asserted-by":"publisher","first-page":"4327","DOI":"10.1016\/j.biomaterials.2011.02.052","volume":"32","author":"Y Yamada","year":"2011","unstructured":"Yamada Y, Katagiri F, Hozumi K, Kikkawa Y, Nomizu M. Cell behavior on protein matrices containing laminin \u03b11 peptide AG73. Biomaterials. 2011;32:4327\u201335.","journal-title":"Biomaterials"},{"key":"815_CR17","doi-asserted-by":"publisher","first-page":"148709","DOI":"10.1016\/j.gene.2024.148709","volume":"927","author":"J Tang","year":"2024","unstructured":"Tang J, Huang X. Transcriptome analysis of human dental pulp cells cultured on a novel cell-adhesive fragment by RNA sequencing. Gene. 2024;927:148709.","journal-title":"Gene"},{"key":"815_CR18","doi-asserted-by":"publisher","first-page":"1385","DOI":"10.1111\/iej.13967","volume":"56","author":"J Tang","year":"2023","unstructured":"Tang J, Wang H, Wu D, Wang Z. LAMA5-inspired adhesive dodecapeptide facilitates efficient dentine regeneration: an in vitro and in vivo study. Int Endod J. 2023;56:1385\u201398.","journal-title":"Int Endod J"},{"key":"815_CR19","doi-asserted-by":"publisher","first-page":"787","DOI":"10.1002\/JLB.2MR0220-549R","volume":"108","author":"TM Bui","year":"2020","unstructured":"Bui TM, Wiesolek HL, Sumagin R. ICAM-1: a master regulator of cellular responses in inflammation, injury resolution, and tumorigenesis. J Leukoc Biol. 2020;108:787\u201399.","journal-title":"J Leukoc Biol"},{"key":"815_CR20","doi-asserted-by":"crossref","unstructured":"Troncoso MF, Ortiz-Quintero J, Garrido-Moreno V, Sanhueza-Olivares F, Guerrero-Moncayo A, Chiong M, et al. VCAM-1 as a predictor biomarker in cardiovascular disease. Biochim Biophys Acta Mol Basis Dis. 2021 ;1867(9):166170.","DOI":"10.1016\/j.bbadis.2021.166170"},{"key":"815_CR21","doi-asserted-by":"publisher","first-page":"1034","DOI":"10.1016\/j.sjbs.2016.12.019","volume":"24","author":"A Sabir","year":"2017","unstructured":"Sabir A, Sumidarti A. Interleukin-6 expression on inflamed rat dental pulp tissue after capped with Trigona sp. propolis from South Sulawesi, Indonesia. Saudi J Biol Sci. 2017;24:1034\u20137.","journal-title":"Saudi J Biol Sci"},{"key":"815_CR22","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1016\/j.cytogfr.2011.10.001","volume":"22","author":"G Lopez-Castejon","year":"2011","unstructured":"Lopez-Castejon G, Brough D. Understanding the mechanism of IL-1\u03b2 secretion. Cytokine Growth Factor Rev. 2011;22:189\u201395.","journal-title":"Cytokine Growth Factor Rev"},{"key":"815_CR23","doi-asserted-by":"publisher","first-page":"339","DOI":"10.1177\/0022034509334070","volume":"88","author":"FWG Paula-Silva","year":"2009","unstructured":"Paula-Silva FWG, Ghosh A, Silva LaB, Kapila YL. TNF-alpha promotes an odontoblastic phenotype in dental pulp cells. J Dent Res. 2009;88:339\u201344.","journal-title":"J Dent Res"},{"key":"815_CR24","unstructured":"The business research community. https:\/\/www.thebusinessresearchcompany.com\/report\/root-canal-global-market-report."},{"key":"815_CR25","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1186\/s12951-025-03266-5","volume":"23","author":"J Wan","year":"2025","unstructured":"Wan J, Xu Y, Qi T, Xue X, Li Y, Huang M, et al. AG73-GelMA\/AlgMA hydrogels provide a stable microenvironment for the generation of pancreatic progenitor organoids. J Nanobiotechnology. 2025;23:149.","journal-title":"J Nanobiotechnology"},{"key":"815_CR26","doi-asserted-by":"publisher","first-page":"1072","DOI":"10.1248\/bpb.b24-00030","volume":"47","author":"Y Yamada","year":"2024","unstructured":"Yamada Y. Characterization of novel cell-adhesive peptides for biomaterial development. Biol Pharm Bull. 2024;47:1072\u20138.","journal-title":"Biol Pharm Bull"},{"key":"815_CR27","doi-asserted-by":"publisher","first-page":"4959","DOI":"10.1038\/s41598-022-08876-9","volume":"12","author":"Y Takechi-Haraya","year":"2022","unstructured":"Takechi-Haraya Y, Ohgita T, Kotani M, Kono H, Saito C, Tamagaki-Asahina H, et al. Effect of hydrophobic moment on membrane interaction and cell penetration of apolipoprotein E-derived arginine-rich amphipathic \u03b1-helical peptides. Sci Rep. 2022;12:4959.","journal-title":"Sci Rep"},{"key":"815_CR28","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/9192516","volume":"2019","author":"M Puchalapalli","year":"2019","unstructured":"Puchalapalli M, Mu L, Edwards C, Kaplan-Singer B, Eni P, Belani K, et al. The Laminin-\u03b11 chain-derived peptide, AG73, binds to syndecans on MDA-231 breast cancer cells and alters filopodium formation. Anal Cell Pathol. 2019;2019:9192516.","journal-title":"Anal Cell Pathol"},{"key":"815_CR29","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2025.108294","volume":"301","author":"AA Vasconcelos","year":"2025","unstructured":"Vasconcelos AA, Zingali RB, Almeida FCL. Surface hydrophobic clusters modulate the folding stability and molecular recognition of the disintegrin jarastatin. J Biol Chem. 2025;301:108294.","journal-title":"J Biol Chem"},{"key":"815_CR30","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1038\/sj.cr.7290105","volume":"12","author":"W Zhang","year":"2002","unstructured":"Zhang W, Liu HT. MAPK signal pathways in the regulation of cell proliferation in mammalian cells. Cell Res. 2002;12:9\u201318.","journal-title":"Cell Res"},{"key":"815_CR31","doi-asserted-by":"crossref","unstructured":"Rodr\u00edguez-Carballo E, G\u00e1mez B, Ventura F. p38 MAPK Signaling in osteoblast differentiation. Front Cell Dev Biol. 2016;4:40.","DOI":"10.3389\/fcell.2016.00040"},{"key":"815_CR32","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1016\/S8756-3282(01)00660-3","volume":"30","author":"A Suzuki","year":"2002","unstructured":"Suzuki A, Guicheux J, Palmer G, Miura Y, Oiso Y, Bonjour JP, et al. Evidence for a role of p38 MAP kinase in expression of alkaline phosphatase during osteoblastic cell differentiation. Bone. 2002;30:91\u20138.","journal-title":"Bone"},{"key":"815_CR33","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1042\/BCJ20200661","volume":"478","author":"MP Damasio","year":"2021","unstructured":"Damasio MP, Marchingo JM, Spinelli L, Hukelmann JL, Cantrell DA, Howden AJM. Extracellular signal-regulated kinase (ERK) pathway control of CD8+ T cell differentiation. Biochem J. 2021;478:79\u201398.","journal-title":"Biochem J"},{"key":"815_CR34","doi-asserted-by":"publisher","first-page":"2613","DOI":"10.3390\/ijms21072613","volume":"21","author":"T Semba","year":"2020","unstructured":"Semba T, Sammons R, Wang X, Xie X, Dalby KN, Ueno NT. JNK signaling in stem cell self-renewal and differentiation. Int J Mol Sci. 2020;21:2613.","journal-title":"Int J Mol Sci"}],"container-title":["Tissue Engineering and Regenerative Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s13770-026-00815-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s13770-026-00815-3","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s13770-026-00815-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T18:04:13Z","timestamp":1785521053000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s13770-026-00815-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,1]]},"references-count":34,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["815"],"URL":"https:\/\/doi.org\/10.1007\/s13770-026-00815-3","relation":{},"ISSN":["1738-2696","2212-5469"],"issn-type":[{"value":"1738-2696","type":"print"},{"value":"2212-5469","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,1]]},"assertion":[{"value":"24 December 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 March 2026","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"17 April 2026","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 July 2026","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare that there is no conflict of interest with regard to the publication of this manuscript.","order":1,"name":"Ethics","label":"Conflict of interest","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All experiments involving human dental pulp cells (hDPCs) were conducted in accordance with protocols approved by the Institutional Ethics Committee of the School of Stomatology, Tongji University (Approval No. 2022-19).","order":2,"name":"Ethics","label":"Ethical approval","group":{"name":"EthicsHeading","label":"Declarations"}}]}}