{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T14:42:13Z","timestamp":1786372933779,"version":"build-2736575974"},"reference-count":39,"publisher":"Springer Science and Business Media LLC","license":[{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Tissue Eng Regen Med"],"DOI":"10.1007\/s13770-026-00832-2","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T13:39:50Z","timestamp":1786369190000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Pilot Study for Establishing DSS-Induced Colitis Model in Minipigs"],"prefix":"10.1007","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-5948-5960","authenticated-orcid":false,"given":"Hye Won","family":"Park","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7281-6322","authenticated-orcid":false,"given":"Woon Kyu","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,8,10]]},"reference":[{"key":"832_CR1","doi-asserted-by":"publisher","DOI":"10.1016\/j.disamonth.2019.02.004","volume":"65","author":"M Gajendran","year":"2019","unstructured":"Gajendran M, Loganathan P, Jimenez G, Catinella AP, Ng N, Umapathy C, et al. A comprehensive review and update on ulcerative colitis. Dis Mon. 2019;65:100851.","journal-title":"Dis Mon"},{"key":"832_CR2","doi-asserted-by":"publisher","first-page":"458","DOI":"10.1038\/s41586-025-08940-0","volume":"642","author":"L Hracs","year":"2025","unstructured":"Hracs L, Windsor JW, Gorospe J, Cummings M, Coward S, Buie MJ, et al. Global evolution of inflammatory bowel disease across epidemiologic stages. Nature. 2025;642:458\u201366.","journal-title":"Nature"},{"key":"832_CR3","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1038\/nature10209","volume":"474","author":"B Khor","year":"2011","unstructured":"Khor B, Gardet A, Xavier RJ. Genetics and pathogenesis of inflammatory bowel disease. Nature. 2011;474:307\u201317.","journal-title":"Nature"},{"key":"832_CR4","doi-asserted-by":"publisher","first-page":"1606","DOI":"10.1016\/S0140-6736(12)60150-0","volume":"380","author":"I Ord\u00e1s","year":"2012","unstructured":"Ord\u00e1s I, Eckmann L, Talamini M, Baumgart DC, Sandborn WJ. Ulcerative colitis. Lancet. 2012;380:1606\u201319.","journal-title":"Lancet"},{"key":"832_CR5","first-page":"1341","volume":"7","author":"D Low","year":"2013","unstructured":"Low D, Nguyen DD, Mizoguchi E. Animal models of ulcerative colitis and their application in drug research. Drug Des Devel Ther. 2013;7:1341\u201357.","journal-title":"Drug Des Devel Ther"},{"key":"832_CR6","doi-asserted-by":"publisher","first-page":"6016","DOI":"10.3748\/wjg.v23.i33.6016","volume":"23","author":"DD Eichele","year":"2017","unstructured":"Eichele DD, Kharbanda KK. Dextran sodium sulfate colitis murine model: an indispensable tool for advancing our understanding of inflammatory bowel diseases pathogenesis. World J Gastroenterol. 2017;23:6016\u201329.","journal-title":"World J Gastroenterol"},{"key":"832_CR7","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0083688","volume":"8","author":"A Ermund","year":"2013","unstructured":"Ermund A, Gustafsson JK, Hansson GC, Keita AV. Mucus properties and goblet cell quantification in mouse, rat and human ileal Peyer\u2019s patches. PLoS ONE. 2013;8:e83688.","journal-title":"PLoS ONE"},{"key":"832_CR8","doi-asserted-by":"publisher","first-page":"104","DOI":"10.3389\/fcimb.2012.00104","volume":"2","author":"N Ottman","year":"2012","unstructured":"Ottman N, Smidt H, de Vos WM. The function of our microbiota: who is out there and what do they do? Front Cell Infect Microbiol. 2012;2:104.","journal-title":"Front Cell Infect Microbiol"},{"key":"832_CR9","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1538\/expanim.49.9","volume":"49","author":"S Kitajima","year":"2000","unstructured":"Kitajima S, Takuma S, Morimoto M. Histological analysis of murine colitis induced by DSS of different molecular weights. Exp Anim. 2000;49:9\u201315.","journal-title":"Exp Anim"},{"key":"832_CR10","doi-asserted-by":"publisher","first-page":"541","DOI":"10.1038\/nprot.2007.41","volume":"2","author":"S Wirtz","year":"2007","unstructured":"Wirtz S, Neufert C, Weigmann B, Neurath MF. Chemically induced mouse models of intestinal inflammation. Nat Protoc. 2007;2:541\u20136.","journal-title":"Nat Protoc"},{"key":"832_CR11","doi-asserted-by":"publisher","DOI":"10.29011\/2577-1701.100041","author":"Y Pyao","year":"2018","unstructured":"Pyao Y, Memon A, Ko TH, Choi HS, Bang BW, Lee WK. Gastric ulcer model in mini-pig. Plast Surg Mod Tech. 2018. https:\/\/doi.org\/10.29011\/2577-1701.100041.","journal-title":"Plast Surg Mod Tech"},{"key":"832_CR12","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2022.834598","volume":"9","author":"EC Rose","year":"2022","unstructured":"Rose EC, Blikslager AT, Ziegler AL. Porcine models of the intestinal microbiota: the translational key to understanding how gut commensals contribute to gastrointestinal disease. Front Vet Sci. 2022;9:834598.","journal-title":"Front Vet Sci"},{"key":"832_CR13","doi-asserted-by":"publisher","first-page":"2099","DOI":"10.1007\/s00726-017-2497-z","volume":"49","author":"L Yin","year":"2017","unstructured":"Yin L, Yang H, Li J, Li Y, Ding X, Wu G, et al. Pig models on intestinal development and therapeutics. Amino Acids. 2017;49:2099\u2013106.","journal-title":"Amino Acids"},{"key":"832_CR14","doi-asserted-by":"publisher","first-page":"2895","DOI":"10.3748\/wjg.v16.i23.2895","volume":"16","author":"A Parra-Blanco","year":"2010","unstructured":"Parra-Blanco A, Arnau MR, Nicol\u00e1s-P\u00e9rez D, Gimeno-Garc\u00eda AZ, Gonz\u00e1lez N, D\u00edaz-Acosta JA, et al. Endoscopic submucosal dissection training with pig models in a western country. World J Gastroenterol. 2010;16:2895\u2013900.","journal-title":"World J Gastroenterol"},{"key":"832_CR15","volume":"97","author":"M Ernst","year":"2015","unstructured":"Ernst M, Preaudet A, Putoczki T. Non-invasive assessment of the efficacy of new therapeutics for intestinal pathologies using serial endoscopic imaging of live mice. J Vis Exp. 2015;97:e52383.","journal-title":"J Vis Exp"},{"key":"832_CR16","unstructured":"Russell WMS, Burch RL. The principles of humane experimental technique. Universities federation for animal welfare. Wheathampstead, UK; 1959"},{"key":"832_CR17","doi-asserted-by":"publisher","first-page":"376","DOI":"10.1038\/s41684-024-01475-3","volume":"53","author":"S Qiu","year":"2024","unstructured":"Qiu S, Jia J, Xu B, Wu N, Cao H, Xie S, et al. Development and evaluation of an autism pig model. Lab Anim. 2024;53:376\u201386.","journal-title":"Lab Anim"},{"key":"832_CR18","doi-asserted-by":"publisher","first-page":"665","DOI":"10.3390\/biom15050665","volume":"15","author":"R Rossi","year":"2025","unstructured":"Rossi R, Mainardi E. Prebiotics and probiotics supplementation in pigs as a model for human gut health and disease. Biomolecules. 2025;15:665.","journal-title":"Biomolecules"},{"key":"832_CR19","doi-asserted-by":"publisher","first-page":"1295","DOI":"10.1038\/nprot.2017.044","volume":"12","author":"S Wirtz","year":"2017","unstructured":"Wirtz S, Popp V, Kindermann M, Gerlach K, Weigmann B, Fichtner-Feigl S, et al. Chemically induced mouse models of acute and chronic intestinal inflammation. Nat Protoc. 2017;12:1295\u2013309.","journal-title":"Nat Protoc"},{"key":"832_CR20","doi-asserted-by":"publisher","first-page":"454","DOI":"10.1016\/j.clnu.2005.12.008","volume":"25","author":"J Bassaganya-Riera","year":"2006","unstructured":"Bassaganya-Riera J, Hontecillas R. CLA and n-3 PUFA differentially modulate clinical activity and colonic PPAR-responsive gene expression in a pig model of experimental IBD. Clin Nutr. 2006;25:454\u201365.","journal-title":"Clin Nutr"},{"key":"832_CR21","doi-asserted-by":"publisher","first-page":"350","DOI":"10.5946\/ce.2012.45.4.350","volume":"45","author":"A Parra-Blanco","year":"2012","unstructured":"Parra-Blanco A, Gonzalez N, Arnau MR. Ex vivo and in vivo models for endoscopic submucosal dissection training. Clin Endosc. 2012;45:350\u20137.","journal-title":"Clin Endosc"},{"key":"832_CR22","first-page":"3678","volume":"60","author":"JJ Kim","year":"2012","unstructured":"Kim JJ, Shajib MS, Manocha MM, Khan WI. Investigating intestinal inflammation in DSS-induced model of IBD. J Vis Exp. 2012;60:3678.","journal-title":"J Vis Exp"},{"key":"832_CR23","first-page":"15.25.1","volume":"104","author":"B Chassaing","year":"2014","unstructured":"Chassaing B, Aitken JD, Malleshappa M, Vijay-Kumar M. Dextran sulfate sodium (DSS)-induced colitis in mice. Curr Protoc Immunol. 2014;104:15.25.1-14.","journal-title":"Curr Protoc Immunol"},{"key":"832_CR24","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0006073","volume":"4","author":"Y Yan","year":"2009","unstructured":"Yan Y, Kolachala V, Dalmasso G, Nguyen H, Laroui H, Sitaraman SV, et al. Temporal and spatial analysis of clinical and molecular parameters in dextran sodium sulfate induced colitis. PLoS ONE. 2009;4:e6073.","journal-title":"PLoS ONE"},{"key":"832_CR25","doi-asserted-by":"publisher","first-page":"74","DOI":"10.1038\/s41572-020-0205-x","volume":"6","author":"T Kobayashi","year":"2020","unstructured":"Kobayashi T, Siegmund B, Le Berre C, Wei SC, Ferrante M, Shen B, et al. Ulcerative colitis. Nat Rev Dis Primers. 2020;6:74.","journal-title":"Nat Rev Dis Primers"},{"key":"832_CR26","doi-asserted-by":"publisher","first-page":"1558092","DOI":"10.3389\/fvets.2025.1558092","volume":"12","author":"V Srivathi","year":"2025","unstructured":"Srivathi V, Doppalapudi M, Yadala RK, Banothu A, Anumolu VK, Veera HDD, et al. Visnagin treatment attenuates DSS-induced colitis by regulating inflammation, oxidative stress, and mucosal damage. Front Vet Sci. 2025;12:1558092.","journal-title":"Front Vet Sci"},{"key":"832_CR27","first-page":"855","volume":"6","author":"C Kozlowski","year":"2013","unstructured":"Kozlowski C, Jeet S, Beyer J, Guerrero S, Lesch J, Wang X, et al. An entirely automated method to score DSS-induced colitis in mice by digital image analysis of pathology slides. Dis Model Mech. 2013;6:855\u201365.","journal-title":"Dis Model Mech"},{"key":"832_CR28","doi-asserted-by":"publisher","first-page":"3193","DOI":"10.21037\/qims-21-1062","volume":"12","author":"D Li","year":"2022","unstructured":"Li D, Ding S, Luo M, Chen J, Zhang Q, Liu Y, et al. Differential diagnosis of acute and chronic colitis in mice by optical coherence tomography. Quant Imaging Med Surg. 2022;12:3193\u2013203.","journal-title":"Quant Imaging Med Surg"},{"key":"832_CR29","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1007\/s10928-021-09789-2","volume":"49","author":"S Kawakatsu","year":"2022","unstructured":"Kawakatsu S, Zhu R, Zhang W, Tang MT, Lu T, Quartino AL, et al. A longitudinal model for the Mayo clinical score and its sub-components in patients with ulcerative colitis. J Pharmacokinet Pharmacodyn. 2022;49:179\u201390.","journal-title":"J Pharmacokinet Pharmacodyn"},{"key":"832_CR30","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2022.846684","volume":"9","author":"S Wang","year":"2022","unstructured":"Wang S, Bai M, Shu Q, Liu Z, Shao Y, Xu K, et al. Modulating effect of paeonol on piglets with ulcerative colitis. Front Nutr. 2022;9:846684.","journal-title":"Front Nutr"},{"key":"832_CR31","doi-asserted-by":"publisher","first-page":"178","DOI":"10.1093\/gastro\/gou031","volume":"2","author":"TC DeRoche","year":"2014","unstructured":"DeRoche TC, Xiao SY, Liu X. Histological evaluation in ulcerative colitis. Gastroenterol Rep. 2014;2:178\u201392.","journal-title":"Gastroenterol Rep"},{"key":"832_CR32","doi-asserted-by":"publisher","first-page":"1262","DOI":"10.3390\/diagnostics13071262","volume":"13","author":"SM Hong","year":"2023","unstructured":"Hong SM, Baek DH. A review of colonoscopy in intestinal diseases. Diagnostics. 2023;13:1262.","journal-title":"Diagnostics"},{"key":"832_CR33","doi-asserted-by":"publisher","first-page":"31","DOI":"10.3390\/medicina57010031","volume":"57","author":"CS Pop","year":"2021","unstructured":"Pop CS, Filip PV, Diaconu SL, Matei C, Furtunescu F. Correlation of biomarkers with endoscopic score: ulcerative colitis endoscopic index of severity (UCEIS) in patients with ulcerative colitis in remission. Medicina. 2021;57:31.","journal-title":"Medicina"},{"key":"832_CR34","doi-asserted-by":"publisher","first-page":"2510","DOI":"10.1016\/j.cgh.2019.12.011","volume":"18","author":"RK Pai","year":"2020","unstructured":"Pai RK, Hartman DJ, Rivers CR, Regeuiro M, Schwartz M, Binion DG, et al. Complete resolution of mucosal neutrophils associates with improved long-term clinical outcomes of patients with ulcerative colitis. Clin Gastroenterol Hepatol. 2020;18:2510\u20137.","journal-title":"Clin Gastroenterol Hepatol"},{"key":"832_CR35","first-page":"1323","volume":"76","author":"RC Langan","year":"2017","unstructured":"Langan RC, Gotsch PB, Krafczyk MA, Skillinge DD. Ulcerative colitis: diagnosis and treatment. Am Fam Physician. 2017;76:1323\u201330.","journal-title":"Am Fam Physician"},{"key":"832_CR36","doi-asserted-by":"publisher","first-page":"535","DOI":"10.1136\/gutjnl-2011-300486","volume":"61","author":"SPL Travis","year":"2012","unstructured":"Travis SPL, Schnell D, Krzeski P, Abreu MT, Altman DG, Colombel J-F, et al. Developing an instrument to assess the endoscopic severity of ulcerative colitis: the ulcerative colitis endoscopic index of severity (UCEIS). Gut. 2012;61:535\u201342.","journal-title":"Gut"},{"key":"832_CR37","doi-asserted-by":"publisher","first-page":"1468394","DOI":"10.3389\/fgstr.2024.1468394","volume":"3","author":"V Bergqvist","year":"2024","unstructured":"Bergqvist V, Gedeon P, Hertervig E, Marsal J. A new simple endoscopic score for ulcerative colitis\u2014the SES-UC. Front Gastroenterol. 2024;3:1468394.","journal-title":"Front Gastroenterol"},{"key":"832_CR38","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0066465","volume":"8","author":"LM Gonzalez","year":"2013","unstructured":"Gonzalez LM, Williamson I, Piedrahita JA, Blikslager AT, Magness ST. Cell lineage identification and stem cell culture in a porcine model for the study of intestinal epithelial regeneration. PLoS ONE. 2013;8:e66465.","journal-title":"PLoS ONE"},{"key":"832_CR39","doi-asserted-by":"publisher","first-page":"1355","DOI":"10.3389\/fgene.2019.01355","volume":"10","author":"KM Summers","year":"2020","unstructured":"Summers KM, Bush SJ, Wu C, Su AI, Muriuki C, Clark EL, et al. Functional annotation of the transcriptome of the pig, Sus scrofa, based upon network analysis of an RNAseq transcriptional atlas. Front Genet. 2020;10:1355.","journal-title":"Front Genet"}],"container-title":["Tissue Engineering and Regenerative Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s13770-026-00832-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s13770-026-00832-2","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s13770-026-00832-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T13:39:53Z","timestamp":1786369193000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s13770-026-00832-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,10]]},"references-count":39,"alternative-id":["832"],"URL":"https:\/\/doi.org\/10.1007\/s13770-026-00832-2","relation":{},"ISSN":["1738-2696","2212-5469"],"issn-type":[{"value":"1738-2696","type":"print"},{"value":"2212-5469","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,10]]},"assertion":[{"value":"8 January 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 June 2026","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 June 2026","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 August 2026","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare that they have no conflict of interest.","order":1,"name":"Ethics","label":"Conflict of interest","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Animal studies were approved by the Animal Research Committee of APURES Co., Ltd., and were maintained and performed according to the guidelines of the Animal Facility (IRB File No. APURES-IACUC-201021-001).","order":2,"name":"Ethics","label":"Ethical approval","group":{"name":"EthicsHeading","label":"Declarations"}}]}}