{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T20:16:11Z","timestamp":1779135371812,"version":"3.51.4"},"reference-count":13,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2023,1,14]],"date-time":"2023-01-14T00:00:00Z","timestamp":1673654400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2023,1,14]],"date-time":"2023-01-14T00:00:00Z","timestamp":1673654400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100002765","name":"Bundesministerium f\u00fcr Wirtschaft und Technologie","doi-asserted-by":"publisher","award":["ZMI 16KN055210"],"award-info":[{"award-number":["ZMI 16KN055210"]}],"id":[{"id":"10.13039\/501100002765","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Universit\u00e4tsklinik M\u00fcnchen"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Infection"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>To analyze sensitivity and specificity of the rapid point-of-care (POC) eazyplex testing platform for bacterial sexually transmitted infections (STI) among men who have sex with men (MSM).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>272 anal, urethral, and pharyngeal swabs collected from 153 MSM were tested by both the eazyplex platform and an in-house PCR or culture in the university microbiology reference laboratory.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Compared to the reference diagnostic method, the overall sensitivity\/specificity of eazyplex was 88%\/98% for\n                      <jats:italic>N. gonorrhoeae<\/jats:italic>\n                      , 82%\/100% for\n                      <jats:italic>C. trachomatis<\/jats:italic>\n                      , 70%\/\u2009&gt;\u200999% for\n                      <jats:italic>U. urealyticum,<\/jats:italic>\n                      and 85%\/98% for\n                      <jats:italic>M. hominis<\/jats:italic>\n                      , respectively. Sensitivity for\n                      <jats:italic>N. gonorrhoeae<\/jats:italic>\n                      and\n                      <jats:italic>U. urealyticum<\/jats:italic>\n                      in urethral samples was 100%.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      With good to very good sensitivity depending on the sampling site and pathogen as well as very good specificity overall the eazyplex platform is a useful rapid diagnostic method for POC bacterial STI-testing especially for\n                      <jats:italic>N. gonorrhoeae<\/jats:italic>\n                      and\n                      <jats:italic>C. trachomatis<\/jats:italic>\n                      , allowing for almost immediate treatment initiation.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s15010-023-01981-5","type":"journal-article","created":{"date-parts":[[2023,1,14]],"date-time":"2023-01-14T14:02:37Z","timestamp":1673704957000},"page":"1141-1145","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Rapid point of care testing for four bacterial sexually transmitted infections using the portable isothermal loop-mediated nucleic acid amplification eazyplex platform"],"prefix":"10.1007","volume":"51","author":[{"given":"Ece T.","family":"Esitgen Germaner","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9754-1185","authenticated-orcid":false,"given":"Lars","family":"Wassill","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5032-8870","authenticated-orcid":false,"given":"Karl","family":"Dichtl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1347-2838","authenticated-orcid":false,"given":"Julia","family":"Roider","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9830-8350","authenticated-orcid":false,"given":"Ulrich","family":"Seybold","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2023,1,14]]},"reference":[{"key":"1981_CR1","unstructured":"World Health Organization. Sexually transmitted infections (STIs) (22 Nov 2021). Available at https:\/\/wwww.hoint\/news-room\/fact-sheets\/detail\/sexually-transmitted-infections-(STIs) Last Accessed on 21 July 2022."},{"key":"1981_CR2","doi-asserted-by":"publisher","first-page":"431","DOI":"10.1016\/s0091-2182(98)00061-5","volume":"43","author":"HV Burst","year":"1998","unstructured":"Burst HV. Sexually transmitted diseases and reproductive health in women. J Nurse Midwifery. 1998;43:431\u201344. https:\/\/doi.org\/10.1016\/s0091-2182(98)00061-5.","journal-title":"J Nurse Midwifery"},{"key":"1981_CR3","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1016\/s0140-6736(98)90002-2","volume":"351","author":"MS Cohen","year":"1998","unstructured":"Cohen MS. Sexually transmitted diseases enhance HIV transmission: no longer a hypothesis. 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Nucleic Acids Res. 2000;28:E63. https:\/\/doi.org\/10.1093\/nar\/28.12.e63.","journal-title":"Nucleic Acids Res"},{"key":"1981_CR6","doi-asserted-by":"publisher","first-page":"e01739","DOI":"10.1128\/JCM.01739-20","volume":"58","author":"T Huber","year":"2020","unstructured":"Huber T, Serr A, Geissdorfer W, Hess C, Lynker-Assmus C, von Loewenich FD, et al. Evaluation of the amplex eazyplex loop-mediated isothermal amplification assay for rapid diagnosis of pneumocystis jirovecii pneumonia. J Clin Microbiol. 2020;58:e01739-e1820. https:\/\/doi.org\/10.1128\/JCM.01739-20.","journal-title":"J Clin Microbiol"},{"key":"1981_CR7","unstructured":"Deutsche STI-Gesellschaft (German STI-Society). Publizierte Leitlinien der DSTIG in Zusammenarbeit mit der Arbeitsgemeinschaft der Wissenschaftlichen Medizinischen Fachgesellschaften (AWMF). Available at https:\/\/www.dstig.de\/literaturleitlinienlinks\/leitlinien.html. 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Lars Wassill is the managing director at Amplex Diagnostics.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"The ethics committee of Medizinische Fakult\u00e4t, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen approved the study (protocol nr. 18\u2013627). The study was performed in accordance with the ethical standards as laid down in the 1964 Declaration of Helsinki and its later amendments.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"Informed consent for publication in a scientific journal was obtained from all individual participants included in the study.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to participate"}}]}}