{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T19:22:52Z","timestamp":1791660172990,"version":"4.3.4"},"reference-count":52,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2024,10,17]],"date-time":"2024-10-17T00:00:00Z","timestamp":1729123200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2024,10,17]],"date-time":"2024-10-17T00:00:00Z","timestamp":1729123200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Infection"],"published-print":{"date-parts":[[2025,6]]},"DOI":"10.1007\/s15010-024-02411-w","type":"journal-article","created":{"date-parts":[[2024,10,17]],"date-time":"2024-10-17T13:02:11Z","timestamp":1729170131000},"page":"967-979","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Targeted next-generation sequencing - a promising approach in the diagnosis of Mycobacterium tuberculosis and drug resistance"],"prefix":"10.1007","volume":"53","author":[{"given":"Xiaocui","family":"Wu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangkun","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunlei","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinghui","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunqiu","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaohui","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangyou","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,10,17]]},"reference":[{"key":"2411_CR1","unstructured":"WHO. Global tuberculosis report 2023. 2023."},{"issue":"2","key":"2411_CR2","doi-asserted-by":"publisher","first-page":"artnoe00038","DOI":"10.1128\/CMR.00038-17","volume":"31","author":"BA Forbes","year":"2018","unstructured":"Forbes BA, Hall GS, Miller MB, et al. Practice guidelines for clinical Microbiology Laboratories: Mycobacteria. Clin Microbiol Rev. 2018;31(2):artnoe00038-17.","journal-title":"Clin Microbiol Rev"},{"issue":"1","key":"2411_CR3","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1016\/S1473-3099(17)30691-6","volume":"18","author":"S Dorman","year":"2018","unstructured":"Dorman S, Schumacher S, Alland D, et al. Xpert MTB\/RIF Ultra for detection of Mycobacterium tuberculosis and rifampicin resistance: a prospective multicentre diagnostic accuracy study. Lancet Infect Dis. 2018;18(1):76\u201384.","journal-title":"Lancet Infect Dis"},{"key":"2411_CR4","unstructured":"WHO. The use of next-generation sequencing for the surveillance of drug-resistant tuberculosis: an implementation manual. 2023."},{"key":"2411_CR5","unstructured":"WHO. Use of targeted next-generation sequencing to detect drug-resistant tuberculosis: rapid communication, July 2023. 2023."},{"key":"2411_CR6","doi-asserted-by":"crossref","unstructured":"Ye J, Huang K, Xu Y, et al. Clinical application of nanopore-targeted sequencing technology in bronchoalveolar lavage fluid from patients with pulmonary infections. Microbiol Spectr. 2024 Apr;30:e0002624.","DOI":"10.1128\/spectrum.00026-24"},{"issue":"4","key":"2411_CR7","doi-asserted-by":"publisher","first-page":"2539","DOI":"10.21037\/jtd-24-400","volume":"16","author":"S Cai","year":"2024","unstructured":"Cai S, Yuan J, Li Y, et al. Etiological diagnostic performance of probe capture-based targeted next-generation sequencing in bloodstream infection. J Thorac Dis. 2024;16(4):2539\u201349.","journal-title":"J Thorac Dis"},{"key":"2411_CR8","doi-asserted-by":"crossref","unstructured":"Lin R, Xing Z, Liu X et al. Performance of targeted next-generation sequencing in the detection of respiratory pathogens and antimicrobial resistance genes for children. J Med Microbiol. 2023;72(11).","DOI":"10.1099\/jmm.0.001771"},{"issue":"4","key":"2411_CR9","doi-asserted-by":"publisher","first-page":"100256","DOI":"10.1016\/j.jfp.2024.100256","volume":"87","author":"A Lin","year":"2024","unstructured":"Lin A, Singh A, Allred A, et al. Targeted next-generation sequencing assay for direct detection and serotyping of Salmonella from Enrichment. J Food Prot. 2024;87(4):100256.","journal-title":"J Food Prot"},{"issue":"11","key":"2411_CR10","doi-asserted-by":"publisher","first-page":"767","DOI":"10.1080\/23744235.2023.2241550","volume":"55","author":"SH Poulsen","year":"2023","unstructured":"Poulsen SH, S\u00f8gaard KK, Fuursted K, Nielsen HL. Evaluating the diagnostic accuracy and clinical utility of 16S and 18S rRNA gene targeted next-generation sequencing based on five years of clinical experience. Infect Dis (Lond). 2023;55(11):767\u201375.","journal-title":"Infect Dis (Lond)"},{"key":"2411_CR11","doi-asserted-by":"publisher","first-page":"2227","DOI":"10.2147\/IDR.S404277","volume":"16","author":"J Li","year":"2023","unstructured":"Li J, Zhang L, Yang X, et al. Diagnostic significance of targeted next-generation sequencing in Central Nervous System infections in Neurosurgery of Pediatrics. Infect Drug Resist. 2023;16:2227\u201336.","journal-title":"Infect Drug Resist"},{"key":"2411_CR12","doi-asserted-by":"crossref","unstructured":"Kyi D, Xiao Y, Wang X, et al. Predominance of A2063G mutant strains in the Mycoplasma pneumoniae epidemic in children-a clinical and epidemiological study in 2023 in Wuhan, China. Int J Infect Dis. 2024 May;9:107074.","DOI":"10.1016\/j.ijid.2024.107074"},{"issue":"3","key":"2411_CR13","doi-asserted-by":"publisher","first-page":"e1456","DOI":"10.1093\/cid\/ciac646","volume":"76","author":"HL Hong","year":"2023","unstructured":"Hong HL, Flurin L, Thoendel MJ, et al. Targeted Versus Shotgun Metagenomic sequencing-based detection of microorganisms in Sonicate Fluid for Periprosthetic Joint infection diagnosis. Clin Infect Dis. 2023;76(3):e1456\u201362.","journal-title":"Clin Infect Dis"},{"issue":"9","key":"2411_CR14","doi-asserted-by":"publisher","first-page":"915","DOI":"10.1111\/crj.13681","volume":"17","author":"Y Zhang","year":"2023","unstructured":"Zhang Y, Jiang X, Ye W, Sun J. Clinical features and outcome of eight patients with Chlamydia psittaci pneumonia diagnosed by targeted next generation sequencing. Clin Respir J. 2023;17(9):915\u201330.","journal-title":"Clin Respir J"},{"key":"2411_CR15","doi-asserted-by":"publisher","first-page":"1303456","DOI":"10.3389\/fcimb.2023.1303456","volume":"13","author":"X Li","year":"2023","unstructured":"Li X, Liu Y, Li M, et al. Epidemiological investigation of lower respiratory tract infections during influenza A (H1N1) pdm09 virus pandemic based on targeted next-generation sequencing. Front Cell Infect Microbiol. 2023;13:1303456.","journal-title":"Front Cell Infect Microbiol"},{"key":"2411_CR16","doi-asserted-by":"publisher","first-page":"1321515","DOI":"10.3389\/fmed.2023.1321515","volume":"10","author":"Z Deng","year":"2023","unstructured":"Deng Z, Li C, Wang Y, et al. Targeted next-generation sequencing for pulmonary infection diagnosis in patients unsuitable for bronchoalveolar lavage. Front Med (Lausanne). 2023;10:1321515.","journal-title":"Front Med (Lausanne)"},{"key":"2411_CR17","doi-asserted-by":"publisher","first-page":"1181348","DOI":"10.3389\/fmicb.2023.1181348","volume":"14","author":"C Huang","year":"2023","unstructured":"Huang C, Huang Y, Wang Z, et al. Multiplex PCR-based next generation sequencing as a novel, targeted and accurate molecular approach for periprosthetic joint infection diagnosis. Front Microbiol. 2023;14:1181348.","journal-title":"Front Microbiol"},{"key":"2411_CR18","unstructured":"WHO. Catalogue of mutations in Mycobacterium tuberculosis complex and their association with drug resistance, 2nd. Second ed. 2023 15 November."},{"key":"2411_CR19","unstructured":"WHO. Catalogue of mutations in Mycobacterium tuberculosis complex and their association with drug resistance, 1nd. 2021."},{"key":"2411_CR20","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1016\/j.ijid.2020.04.039","volume":"96","author":"X Wu","year":"2020","unstructured":"Wu X, Gao R, Shen X, et al. Use of whole-genome sequencing to predict Mycobacterium tuberculosis drug resistance in Shanghai, China. Int J Infect Dis. 2020;96:48\u201353.","journal-title":"Int J Infect Dis"},{"issue":"2","key":"2411_CR21","doi-asserted-by":"publisher","first-page":"e0251621","DOI":"10.1128\/spectrum.02516-21","volume":"10","author":"X Wu","year":"2022","unstructured":"Wu X, Tan G, Sha W, et al. Use of whole-genome sequencing to Predict Mycobacterium tuberculosis Complex Drug Resistance from early positive liquid cultures. Microbiol Spectr. 2022;10(2):e0251621.","journal-title":"Microbiol Spectr"},{"key":"2411_CR22","unstructured":"Industry PsRoCH. Diagnosis of Pulmonary Tuberculosis (WS 288\u20132017). Electron J Emerg Infect Dis2018. pp. 59\u201361."},{"key":"2411_CR23","doi-asserted-by":"publisher","first-page":"345","DOI":"10.3389\/fcimb.2019.00345","volume":"9","author":"X Wu","year":"2019","unstructured":"Wu X, Yang J, Tan G, et al. Drug Resistance characteristics of Mycobacterium tuberculosis isolates from patients with tuberculosis to 12 antituberculous drugs in China. Front Cell Infect Microbiol. 2019;9:345.","journal-title":"Front Cell Infect Microbiol"},{"issue":"17","key":"2411_CR24","doi-asserted-by":"publisher","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","volume":"34","author":"S Chen","year":"2018","unstructured":"Chen S, Zhou Y, Chen Y, Gu J. Fastp: an ultra-fast all-in-one FASTQ preprocessor. Bioinformatics. 2018;34(17):i884\u201390.","journal-title":"Bioinformatics"},{"key":"2411_CR25","unstructured":"Li H. Aligning sequence reads, clone sequences and assembly contigs with BWA-MEM. arXiv e-prints. 2013."},{"issue":"D1","key":"2411_CR26","doi-asserted-by":"publisher","first-page":"D41","DOI":"10.1093\/nar\/gkx1094","volume":"46","author":"DA Benson","year":"2018","unstructured":"Benson DA, Cavanaugh M, Clark K, et al. GenBank Nucleic Acids Res. 2018;46(D1):D41\u20137.","journal-title":"GenBank Nucleic Acids Res"},{"issue":"D1","key":"2411_CR27","doi-asserted-by":"publisher","first-page":"D733","DOI":"10.1093\/nar\/gkv1189","volume":"44","author":"NA O\u2019Leary","year":"2016","unstructured":"O\u2019Leary NA, Wright MW, Brister JR, et al. Reference sequence (RefSeq) database at NCBI: current status, taxonomic expansion, and functional annotation. Nucleic Acids Res. 2016;44(D1):D733\u201345.","journal-title":"Nucleic Acids Res"},{"issue":"6522","key":"2411_CR28","doi-asserted-by":"publisher","first-page":"746","DOI":"10.1136\/bmj.292.6522.746","volume":"292","author":"MJ Gardner","year":"1986","unstructured":"Gardner MJ, Altman DG. Confidence intervals rather than P values: estimation rather than hypothesis testing. Br Med J (Clin Res Ed). 1986;292(6522):746\u201350.","journal-title":"Br Med J (Clin Res Ed)"},{"issue":"6947","key":"2411_CR29","doi-asserted-by":"publisher","first-page":"102","DOI":"10.1136\/bmj.309.6947.102","volume":"309","author":"DG Altman","year":"1994","unstructured":"Altman DG, Bland JM. Diagnostic tests 2: predictive values. BMJ. 1994;309(6947):102.","journal-title":"BMJ"},{"issue":"6943","key":"2411_CR30","doi-asserted-by":"publisher","first-page":"1552","DOI":"10.1136\/bmj.308.6943.1552","volume":"308","author":"DG Altman","year":"1994","unstructured":"Altman DG, Bland JM. Diagnostic tests. 1: sensitivity and specificity. BMJ. 1994;308(6943):1552.","journal-title":"BMJ"},{"key":"2411_CR31","unstructured":"WHO. WHO operational handbook on tuberculosis. Module 3: Diagnosis - Rapid diagnostics for tuberculosis detection. third edition ed. 2024."},{"issue":"6","key":"2411_CR32","doi-asserted-by":"publisher","first-page":"e0260522","DOI":"10.1128\/spectrum.02605-22","volume":"10","author":"SH Wu","year":"2022","unstructured":"Wu SH, Xiao YX, Hsiao HC, Jou R. Development and Assessment of a Novel Whole-Gene-based targeted next-generation sequencing assay for detecting the susceptibility of Mycobacterium tuberculosis to 14 drugs. Microbiol Spectr. 2022;10(6):e0260522.","journal-title":"Microbiol Spectr"},{"key":"2411_CR33","doi-asserted-by":"publisher","first-page":"102051","DOI":"10.1016\/j.tube.2021.102051","volume":"127","author":"P Kambli","year":"2021","unstructured":"Kambli P, Ajbani K, Kazi M, et al. Targeted next generation sequencing directly from sputum for comprehensive genetic information on drug resistant Mycobacterium tuberculosis. Tuberculosis (Edinb). 2021;127:102051.","journal-title":"Tuberculosis (Edinb)"},{"key":"2411_CR34","doi-asserted-by":"crossref","unstructured":"Cabibbe AM, Spitaleri A, Battaglia S et al. Application of targeted next-generation sequencing assay on a portable sequencing platform for culture-free detection of drug-resistant tuberculosis from clinical samples. J Clin Microbiol. 2020;58(10).","DOI":"10.1128\/JCM.00632-20"},{"issue":"1","key":"2411_CR35","doi-asserted-by":"publisher","first-page":"74","DOI":"10.1039\/D3LC00783A","volume":"24","author":"J Schlanderer","year":"2023","unstructured":"Schlanderer J, Hoffmann H, L\u00fcddecke J, et al. Two-stage tuberculosis diagnostics: combining centrifugal microfluidics to detect TB infection and inh and rif resistance at the point of care with subsequent antibiotic resistance profiling by targeted NGS. Lab Chip. 2023;24(1):74\u201384.","journal-title":"Lab Chip"},{"issue":"1","key":"2411_CR36","doi-asserted-by":"publisher","first-page":"e0135821","DOI":"10.1128\/spectrum.01358-21","volume":"10","author":"J Song","year":"2022","unstructured":"Song J, Du W, Liu Z, et al. Application of Amplicon-based targeted NGS technology for diagnosis of drug-resistant tuberculosis using FFPE specimens. Microbiol Spectr. 2022;10(1):e0135821.","journal-title":"Microbiol Spectr"},{"issue":"2","key":"2411_CR37","doi-asserted-by":"publisher","first-page":"43","DOI":"10.5588\/pha.22.0041","volume":"13","author":"A Iyer","year":"2023","unstructured":"Iyer A, Ndlovu Z, Sharma J, et al. Operationalising targeted next-generation sequencing for routine diagnosis of drug-resistant TB. Public Health Action. 2023;13(2):43\u20139.","journal-title":"Public Health Action"},{"issue":"4","key":"2411_CR38","doi-asserted-by":"publisher","first-page":"e0128723","DOI":"10.1128\/jcm.01287-23","volume":"62","author":"TTB Tram","year":"2024","unstructured":"Tram TTB, Trieu LPT, Nhat LTH, et al. Targeted sequencing from cerebrospinal fluid for rapid identification of drug-resistant tuberculous meningitis. J Clin Microbiol. 2024;62(4):e0128723.","journal-title":"J Clin Microbiol"},{"issue":"5","key":"2411_CR39","doi-asserted-by":"publisher","first-page":"e0409823","DOI":"10.1128\/spectrum.04098-23","volume":"12","author":"H Zhang","year":"2024","unstructured":"Zhang H, Dai X, Hu P, et al. Comparison of targeted next-generation sequencing and the Xpert MTB\/RIF assay for detection of Mycobacterium tuberculosis in clinical isolates and sputum specimens. Microbiol Spectr. 2024;12(5):e0409823.","journal-title":"Microbiol Spectr"},{"key":"2411_CR40","doi-asserted-by":"crossref","unstructured":"Genestet C, Baffert Y, Vall\u00e9e M et al. Development, evaluation, and implementation of a House-made targeted next-generation sequencing spoligotyping in a French laboratory. Int J Mol Sci. 2022;23(19).","DOI":"10.3390\/ijms231911302"},{"issue":"1","key":"2411_CR41","doi-asserted-by":"publisher","first-page":"e23217","DOI":"10.1016\/j.heliyon.2023.e23217","volume":"10","author":"H Zheng","year":"2024","unstructured":"Zheng H, Yang H, Wang Y, et al. Diagnostic value of tNGS vs Xpert MTB\/RIF in childhood TB. Heliyon. 2024;10(1):e23217.","journal-title":"Heliyon"},{"issue":"2","key":"2411_CR42","doi-asserted-by":"publisher","first-page":"116249","DOI":"10.1016\/j.diagmicrobio.2024.116249","volume":"109","author":"W Cloutier Charette","year":"2024","unstructured":"Cloutier Charette W, Rabodoarivelo MS, Point F, et al. Concordance of targeted and whole genome sequencing for Mycobacterium tuberculosis genotypic drug susceptibility testing. Diagn Microbiol Infect Dis. 2024;109(2):116249.","journal-title":"Diagn Microbiol Infect Dis"},{"issue":"1","key":"2411_CR43","doi-asserted-by":"publisher","first-page":"52","DOI":"10.1186\/s13073-022-01054-6","volume":"14","author":"DB Sibandze","year":"2022","unstructured":"Sibandze DB, Kay A, Dreyer V, et al. Rapid molecular diagnostics of tuberculosis resistance by targeted stool sequencing. Genome Med. 2022;14(1):52.","journal-title":"Genome Med"},{"key":"2411_CR44","doi-asserted-by":"publisher","first-page":"1206056","DOI":"10.3389\/fpubh.2023.1206056","volume":"11","author":"SG Murphy","year":"2023","unstructured":"Murphy SG, Smith C, Lapierre P, et al. Direct detection of drug-resistant Mycobacterium tuberculosis using targeted next generation sequencing. Front Public Health. 2023;11:1206056.","journal-title":"Front Public Health"},{"issue":"3","key":"2411_CR45","first-page":"e19","volume":"3","author":"J Li","year":"2014","unstructured":"Li J, Gao X, Luo T, et al. Association of gyrA\/B mutations and resistance levels to fluoroquinolones in clinical isolates of Mycobacterium tuberculosis. Emerg Microbes Infect. 2014;3(3):e19.","journal-title":"Emerg Microbes Infect"},{"issue":"4","key":"2411_CR46","doi-asserted-by":"publisher","first-page":"1233","DOI":"10.1128\/JCM.06479-11","volume":"50","author":"PK Angra","year":"2012","unstructured":"Angra PK, Taylor TH, Iademarco MF, et al. Performance of tuberculosis drug susceptibility testing in U.S. laboratories from 1994 to 2008. J Clin Microbiol. 2012;50(4):1233\u20139.","journal-title":"J Clin Microbiol"},{"issue":"4","key":"2411_CR47","doi-asserted-by":"publisher","first-page":"e0152926","DOI":"10.1371\/journal.pone.0152926","volume":"11","author":"V Nikolayevskyy","year":"2016","unstructured":"Nikolayevskyy V, Hillemann D, Richter E, et al. External Quality Assessment for Tuberculosis diagnosis and Drug Resistance in the European Union: a five year multicentre implementation study. PLoS ONE. 2016;11(4):e0152926.","journal-title":"PLoS ONE"},{"issue":"11","key":"2411_CR48","doi-asserted-by":"publisher","first-page":"3976","DOI":"10.1128\/JCM.40.11.3976-3979.2002","volume":"40","author":"B Madison","year":"2002","unstructured":"Madison B, Robinson-Dunn B, George I, et al. Multicenter evaluation of ethambutol susceptibility testing of mycobacterium tuberculosis by agar proportion and radiometric methods. J Clin Microbiol. 2002;40(11):3976\u20139.","journal-title":"J Clin Microbiol"},{"issue":"1","key":"2411_CR49","first-page":"116","volume":"15","author":"A Van Deun","year":"2011","unstructured":"Van Deun A, Wright A, Zignol M, et al. Drug susceptibility testing proficiency in the network of supranational tuberculosis reference laboratories. Int J Tuberc Lung Dis. 2011;15(1):116\u201324.","journal-title":"Int J Tuberc Lung Dis"},{"issue":"1","key":"2411_CR50","doi-asserted-by":"publisher","first-page":"e0226922","DOI":"10.1128\/spectrum.02269-22","volume":"11","author":"TE Ness","year":"2023","unstructured":"Ness TE, Meiwes L, Kay A, et al. Optimizing DNA extraction from Pediatric Stool for diagnosis of tuberculosis and use in next-generation sequencing applications. Microbiol Spectr. 2023;11(1):e0226922.","journal-title":"Microbiol Spectr"},{"key":"2411_CR51","unstructured":"WHO. WHO operational handbook on tuberculosis. Module 4: treatment - drug-resistant tuberculosis treatment, 2022 update. 2022."},{"key":"2411_CR52","unstructured":"WHO. Use of targeted next-generation sequencing to detect drug-resistant tuberculosis: rapid communication. 2023."}],"container-title":["Infection"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s15010-024-02411-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s15010-024-02411-w\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s15010-024-02411-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,4]],"date-time":"2025-06-04T01:54:32Z","timestamp":1749002072000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s15010-024-02411-w"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,17]]},"references-count":52,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,6]]}},"alternative-id":["2411"],"URL":"https:\/\/doi.org\/10.1007\/s15010-024-02411-w","relation":{},"ISSN":["0300-8126","1439-0973"],"issn-type":[{"value":"0300-8126","type":"print"},{"value":"1439-0973","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,17]]},"assertion":[{"value":"24 July 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 September 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"17 October 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"This study was approved by the Ethical Committee of Shanghai Pulmonary Hospital.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}