{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T20:56:47Z","timestamp":1791061007681,"version":"4.1.0"},"reference-count":11,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T00:00:00Z","timestamp":1743984000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T00:00:00Z","timestamp":1743984000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Universit\u00e4tsklinikum Frankfurt"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Infection"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Objectives<\/jats:title>\n            <jats:p>\n              <jats:italic>Mycobacterium chelonae<\/jats:italic> is a rapid-growing non-tuberculous mycobacterium that has occasionally been described in connection with foreign material infections, e.g. after orthopaedic joint replacement or cosmetic surgery. In a recent outbreak, several cases of <jats:italic>M. chelonae<\/jats:italic> endocarditis associated with biological heart valve prostheses were reported.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Case history<\/jats:title>\n            <jats:p>A 64-year-old female patient with a history of myalgia and recurrent joint swelling presented to our hospital. Initially suspected for rheumatoid arthritis, the patient underwent a series of orthopedic and rheumatologic treatments, including prednisolone and methotrexate. Subsequent history revealed a Ross operation in 2014 and a PET-CT was suspicious of a biological valved conduit infection leading to surgical replacement. Utilizing fluorescence in situ hybridization (FISH) diagnostic techniques, DAPI, Kinyoun and Ziehl-Neelsen staining, mycobacterial infection was confirmed in both the prosthesis and adjacent muscle tissue. Molecular methods identified a mycobacterium most closely related to the <jats:italic>M. chelonae\/abscessus<\/jats:italic> complex indicating an association to a previously described outbreak of <jats:italic>M. chelonae<\/jats:italic> contaminated heart valves. Antimycobacterial therapy was initiated and the patient remains stable at the time of writing. To date, all mycobacterial cultures remained negative.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>Non-tuberculous mycobacteria (NTM) are rare and possibly underdiagnosed pathogens in infections of bioprosthetic flap bearing conduits. Mycobacterial foreign-body infections can manifest many years after implantation. As NTM can be difficult to detect, molecular identification methods are of particular importance. Here, modern imaging, molecular and microscopic techniques might be of special use in diagnosing prolonged prosthetic graft infections.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1007\/s15010-025-02534-8","type":"journal-article","created":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T04:38:49Z","timestamp":1744000729000},"page":"1495-1499","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Mycobacterium chelonae infection of a cardiovascular bioprosthesis linked to a recent outbreak"],"prefix":"10.1007","volume":"53","author":[{"given":"Johanna","family":"Kessel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Axel","family":"Braner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margo","family":"Diricks","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Walther","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomas","family":"Holubec","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rudolf","family":"Werner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Hogardt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas A.","family":"Wichelhaus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Niemann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annette","family":"Moter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inna","family":"Friesen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5589-4668","authenticated-orcid":false,"given":"Nils","family":"Wetzstein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,4,7]]},"reference":[{"issue":"6","key":"2534_CR1","doi-asserted-by":"publisher","first-page":"1050","DOI":"10.1093\/cid\/ciac860","volume":"76","author":"MM Hajduczenia","year":"2023","unstructured":"Hajduczenia MM, Klefisch FR, Hopf AGM, Grubitzsch H, Stegemann MS, Pf\u00e4fflin F, Puhlmann B, Ocken M, Kretzler L, von Sch\u00f6ning D, Falk V, Moter A, Kikhney J. New perspectives for prosthetic valve endocarditis: impact of molecular imaging by FISHseq diagnostics. Clin Infect Dis. 2023;76(6):1050\u20138. https:\/\/doi.org\/10.1093\/cid\/ciac860. PMID: 36318608; PMCID: PMC10029985.","journal-title":"Clin Infect Dis"},{"issue":"10","key":"2534_CR2","doi-asserted-by":"publisher","first-page":"3760","DOI":"10.1128\/JCM.01435-06","volume":"44","author":"M Lefmann","year":"2006","unstructured":"Lefmann M, Schweickert B, Buchholz P, G\u00f6bel UB, Ulrichs T, Seiler P, Theegarten D, Moter A. Evaluation of peptide nucleic acid-fluorescence in situ hybridization for identification of clinically relevant mycobacteria in clinical specimens and tissue sections. J Clin Microbiol. 2006;44(10):3760\u20137. https:\/\/doi.org\/10.1128\/JCM.01435-06. 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PMID: 18034202.","journal-title":"Clin Rheumatol"},{"issue":"1","key":"2534_CR5","doi-asserted-by":"publisher","first-page":"e16","DOI":"10.1016\/j.bjps.2012.09.028","volume":"66","author":"M Thomas","year":"2013","unstructured":"Thomas M, D\u2019Silva JA, Borole AJ, Chilgar RM. Periprosthetic atypical mycobacterial infection in breast implants: a new kid on the block! J Plast Reconstr Aesthet Surg. 2013;66(1):e16\u20139. https:\/\/doi.org\/10.1016\/j.bjps.2012.09.028. Epub 2012 Nov 2. PMID: 23122691.","journal-title":"J Plast Reconstr Aesthet Surg"},{"key":"2534_CR6","doi-asserted-by":"crossref","unstructured":"B\u00fcchler AC, Lazarevic V, Ga\u00efa N, Girard M, Eckstein F, Egli A et al. Mycobacterium chelonae infection identified by metagenomic Next-Generation sequencing as the probable cause of acute contained rupture of a biological composite graft. A case report. Int J Mol Sci 2021; 23(1).","DOI":"10.3390\/ijms23010381"},{"key":"2534_CR7","unstructured":"https:\/\/www.dgthg.de\/sites\/default\/files\/220607_Information_MchelonaeBioIntergral_final.pdf."},{"key":"2534_CR8","doi-asserted-by":"publisher","first-page":"100934","DOI":"10.1016\/j.lanmic.2024.06.001","volume":"0","author":"J Kikhney","year":"2024","unstructured":"Kikhney J, Friesen I, Wiesener S, Kursawe L, Loddenkemper C, Z\u00fcndorf J, et al. Endocarditis associated with contamination of cardiovascular bioprostheses with Mycobacterium Chelonae: a collaborative Microbiological study. 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Clin Microbiol Infect. 2023. https:\/\/doi.org\/10.1016\/j.cmi.2023.03.005.","DOI":"10.1016\/j.cmi.2023.03.005"}],"container-title":["Infection"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s15010-025-02534-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s15010-025-02534-8\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s15010-025-02534-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,6]],"date-time":"2025-09-06T10:14:23Z","timestamp":1757153663000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s15010-025-02534-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,7]]},"references-count":11,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,8]]}},"alternative-id":["2534"],"URL":"https:\/\/doi.org\/10.1007\/s15010-025-02534-8","relation":{},"ISSN":["0300-8126","1439-0973"],"issn-type":[{"value":"0300-8126","type":"print"},{"value":"1439-0973","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,7]]},"assertion":[{"value":"13 January 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"30 March 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 April 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The patient signed informed consent for publication of her case history.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics, consent to participate and consent to publication"}},{"value":"Johanna Kessel, Axel Braner, Margo Diricks, Thomas Walther, Michael Hogardt, Stefan Niemann, Inna Friesen, and Nils Wetzstein have nothing to disclose.Tomas Holubec has received consultancy fees and honoraria from Abbott, USA; Artivion, USA; Fehling, Germany and Getinge, Sweden, outside the submitted work. Rudolf Werner has received speaker honoraria from Novartis\/AAA and PentixaPharm, reports advisory board work for Novartis\/AAA and Bayer and in involved in 68Ga-PentixaFor PET Imaging in PAN Cancer (FORPAN), sponsored and planned by PentixaPharm. Thomas A. Wichelhaus reports research grants from BMBF, JPIAMR, Deutsche Krebshilfe, MSD as well as speaker fees and consulting honoraria from Landes\u00e4rztekammer Hessen, Insmed, Osartis, all outside the submitted work. Annette Moter holds shares in MiKi Analytics GmbH and runs Moter Diagnostics private practice.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}