{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T17:58:20Z","timestamp":1784138300422,"version":"3.55.0"},"reference-count":25,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2024,3,1]],"date-time":"2024-03-01T00:00:00Z","timestamp":1709251200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0"},{"start":{"date-parts":[[2024,3,8]],"date-time":"2024-03-08T00:00:00Z","timestamp":1709856000000},"content-version":"vor","delay-in-days":7,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Infect Dis Ther"],"published-print":{"date-parts":[[2024,3]]},"DOI":"10.1007\/s40121-024-00934-1","type":"journal-article","created":{"date-parts":[[2024,3,8]],"date-time":"2024-03-08T17:02:05Z","timestamp":1709917325000},"page":"625-632","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Amoebic Liver Abscess: Potential Application of New Diagnostic Techniques for an Old Pathogen"],"prefix":"10.1007","volume":"13","author":[{"given":"Federica","family":"Portunato","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-1940-5620","authenticated-orcid":false,"given":"Claudia","family":"Bartalucci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Vena","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniele Roberto","family":"Giacobbe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisa","family":"Porcile","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Montanelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nataniele","family":"Piol","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica","family":"Centanaro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enzo","family":"Andorno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matteo","family":"Bassetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,3,8]]},"reference":[{"issue":"1","key":"934_CR1","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1016\/j.jinf.2009.05.008","volume":"59","author":"A Swaminathan","year":"2009","unstructured":"Swaminathan A, Torresi J, Schlagenhauf P, et al. A global study of pathogens and host risk factors associated with infectious gastrointestinal disease in returned international travellers. J Infect. 2009;59(1):19\u201327. https:\/\/doi.org\/10.1016\/j.jinf.2009.05.008.","journal-title":"J Infect"},{"key":"934_CR2","unstructured":"CDC Yellow Book 2024. Amebiasis. Available at: https:\/\/wwwnc.cdc.gov\/travel\/yellowbook\/2024\/infections-diseases\/amebiasis. Accessed 29 Feb 2024."},{"issue":"9362","key":"934_CR3","doi-asserted-by":"publisher","first-page":"1025","DOI":"10.1016\/S0140-6736(03)12830-9","volume":"361","author":"SL Stanley","year":"2003","unstructured":"Stanley SL. Amoebiasis. Lancet. 2003;361(9362):1025\u201334. https:\/\/doi.org\/10.1016\/S0140-6736(03)12830-9.","journal-title":"Lancet"},{"key":"934_CR4","doi-asserted-by":"publisher","unstructured":"Shirley DT, Farr L, Watanabe K, Moonah S. A review of the global burden, new diagnostics, and current therapeutics for amebiasis. Open Forum Infect Dis. 2018;5(7):ofy161. https:\/\/doi.org\/10.1093\/ofid\/ofy161.","DOI":"10.1093\/ofid\/ofy161"},{"issue":"1","key":"934_CR5","doi-asserted-by":"publisher","first-page":"364","DOI":"10.1186\/s12879-022-07348-9","volume":"22","author":"B Fu","year":"2022","unstructured":"Fu B, Wang J, Fu X. A rare case of extraintestinal amebiasis. BMC Infect Dis. 2022;22(1):364. https:\/\/doi.org\/10.1186\/s12879-022-07348-9.","journal-title":"BMC Infect Dis"},{"key":"934_CR6","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1051\/parasite\/2015020","volume":"22","author":"B Nespola","year":"2015","unstructured":"Nespola B, Betz V, Brunet J, et al. 2015. First case of amebic liver abscess 22\u00a0years after the first occurrence. Parasite. 2015;22:20. https:\/\/doi.org\/10.1051\/parasite\/2015020.","journal-title":"Parasite"},{"issue":"1","key":"934_CR7","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2019.151358","volume":"310","author":"JC Carrero","year":"2020","unstructured":"Carrero JC, Reyes-L\u00f3pez M, Serrano-Luna J, et al. 2020. Intestinal amoebiasis: 160\u00a0years of its first detection and still remains as a health problem in developing countries. Int J Med Microbiol. 2020;310(1):151358. https:\/\/doi.org\/10.1016\/j.ijmm.2019.151358.","journal-title":"Int J Med Microbiol"},{"issue":"2","key":"934_CR8","doi-asserted-by":"publisher","first-page":"361","DOI":"10.1016\/j.gtc.2020.01.013","volume":"49","author":"R Roediger","year":"2020","unstructured":"Roediger R, Lisker-Melman M. Pyogenic and amebic infections of the liver. Gastroenterol Clin North Am. 2020;49(2):361\u201377. https:\/\/doi.org\/10.1016\/j.gtc.2020.01.013.","journal-title":"Gastroenterol Clin North Am"},{"key":"934_CR9","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1155\/2018\/4601420","volume":"2018","author":"M Kantor","year":"2018","unstructured":"Kantor M, Abrantes A, Estevez A, et al. Entamoeba histolytica: updates in clinical manifestation, pathogenesis, and vaccine development. Can J Gastroenterol Hepatol. 2018;2018:1\u20136. https:\/\/doi.org\/10.1155\/2018\/4601420.","journal-title":"Can J Gastroenterol Hepatol"},{"issue":"1","key":"934_CR10","doi-asserted-by":"publisher","first-page":"2158656","DOI":"10.1080\/21505594.2022.2158656","volume":"14","author":"N Guill\u00e9n","year":"2023","unstructured":"Guill\u00e9n N. Pathogenicity and virulence of Entamoeba histolytica, the agent of amoebiasis. Virulence. 2023;14(1):2158656. https:\/\/doi.org\/10.1080\/21505594.2022.2158656.","journal-title":"Virulence"},{"issue":"2","key":"934_CR11","doi-asserted-by":"publisher","first-page":"481","DOI":"10.1016\/j.jhep.2021.03.018","volume":"75","author":"J Sellau","year":"2021","unstructured":"Sellau J, Groneberg M, Hoenow S, Lotter H. The underlying cellular immune pathology of Entamoeba histolytica-induced hepatic amoebiasis. J Hepatol. 2021;75(2):481\u20132. https:\/\/doi.org\/10.1016\/j.jhep.2021.03.018.","journal-title":"J Hepatol"},{"issue":"1","key":"934_CR12","doi-asserted-by":"publisher","first-page":"105","DOI":"10.2217\/17460913.4.1.105","volume":"4","author":"M Lejeune","year":"2009","unstructured":"Lejeune M, Rybicka JM, Chadee K. Recent discoveries in the pathogenesis and immune response toward Entamoeba histolytica. Future Microbiol. 2009;4(1):105\u201318. https:\/\/doi.org\/10.2217\/17460913.4.1.105.","journal-title":"Future Microbiol"},{"issue":"1","key":"934_CR13","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1007\/s10096-018-3379-3","volume":"38","author":"S Saidin","year":"2019","unstructured":"Saidin S, Othman N, Noordin R. Update on laboratory diagnosis of amoebiasis. Eur J Clin Microbiol Infect Dis. 2019;38(1):15\u201338. https:\/\/doi.org\/10.1007\/s10096-018-3379-3.","journal-title":"Eur J Clin Microbiol Infect Dis"},{"issue":"4","key":"934_CR14","doi-asserted-by":"publisher","first-page":"713","DOI":"10.1128\/CMR.16.4.713-729.2003","volume":"16","author":"M Tanyuksel","year":"2003","unstructured":"Tanyuksel M, Petri WA. Laboratory diagnosis of amebiasis. Clin Microbiol Rev. 2003;16(4):713\u201329. https:\/\/doi.org\/10.1128\/CMR.16.4.713-729.2003.","journal-title":"Clin Microbiol Rev"},{"issue":"2","key":"934_CR15","doi-asserted-by":"publisher","first-page":"190.e1","DOI":"10.1016\/j.cmi.2015.10.019","volume":"22","author":"A Laude","year":"2016","unstructured":"Laude A, et al. Is real-time PCR-based diagnosis similar in performance to routine parasitological examination for the identification of Giardia intestinalis, Cryptosporidium parvum\/Cryptosporidium hominis and Entamoeba histolytica from stool samples? Evaluation of a new commercial multiplex PCR assay and literature review. Clin Microbiol Infect. 2016;22(2):190.e1-190.e8. https:\/\/doi.org\/10.1016\/j.cmi.2015.10.019.","journal-title":"Clin Microbiol Infect"},{"issue":"1","key":"934_CR16","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1016\/j.exppara.2006.11.005","volume":"116","author":"J Paul","year":"2007","unstructured":"Paul J, Srivastava S, Bhattacharya S. Molecular methods for diagnosis of Entamoeba histolytica in a clinical setting: an overview. Exp Parasitol. 2007;116(1):35\u201343. https:\/\/doi.org\/10.1016\/j.exppara.2006.11.005.","journal-title":"Exp Parasitol"},{"issue":"36","key":"934_CR17","doi-asserted-by":"publisher","first-page":"13157","DOI":"10.12998\/wjcc.v10.i36.13157","volume":"10","author":"D Usuda","year":"2022","unstructured":"Usuda D, Tsuge S, Sakurai R, et al. Amebic liver abscess by Entamoeba histolytica. World J Clin Cases. 2022;10(36):13157\u201366. https:\/\/doi.org\/10.12998\/wjcc.v10.i36.13157.","journal-title":"World J Clin Cases"},{"issue":"8","key":"934_CR18","doi-asserted-by":"publisher","first-page":"719","DOI":"10.1016\/j.cmi.2015.04.007","volume":"21","author":"A Spina","year":"2015","unstructured":"Spina A, Kerr KG, Cormican M, et al. Spectrum of enteropathogens detected by the FilmArray GI panel in a multicentre study of community-acquired gastroenteritis. Clin Microbiol Infect. 2015;21(8):719\u201328. https:\/\/doi.org\/10.1016\/j.cmi.2015.04.007.","journal-title":"Clin Microbiol Infect"},{"issue":"3","key":"934_CR19","doi-asserted-by":"publisher","first-page":"915","DOI":"10.1128\/JCM.02674-14","volume":"53","author":"SN Buss","year":"2015","unstructured":"Buss SN, Leber A, Chapin K, et al. Multicenter evaluation of the BioFire FilmArray gastrointestinal panel for etiologic diagnosis of infectious gastroenteritis. J Clin Microbiol. 2015;53(3):915\u201325. https:\/\/doi.org\/10.1128\/JCM.02674-14.","journal-title":"J Clin Microbiol"},{"key":"934_CR20","unstructured":"bioM\u00e9rieux BIOFIRE\u00ae FILMARRAY\u00ae Gastrointestinal (GI) Panel. Available at: https:\/\/www.biomerieux.com\/us\/en\/our-offer\/products\/biofire-gastrointestinal-panel.html. Accessed 29 Feb 2024."},{"issue":"16","key":"934_CR21","doi-asserted-by":"publisher","first-page":"1565","DOI":"10.1056\/NEJMra022710","volume":"348","author":"R Haque","year":"2003","unstructured":"Haque R, Huston CD, Hughes M, Houpt E, Petri WA. Amebiasis. N Engl J Med. 2003;348(16):1565\u201373. https:\/\/doi.org\/10.1056\/NEJMra022710.","journal-title":"N Engl J Med"},{"issue":"5","key":"934_CR22","doi-asserted-by":"publisher","first-page":"893","DOI":"10.1586\/14787210.5.5.893","volume":"5","author":"JM Salles","year":"2007","unstructured":"Salles JM, Salles MJ, Moraes LA, Silva MC. Invasive amebiasis: an update on diagnosis and management. Expert Rev Anti Infect Ther. 2007;5(5):893\u2013901. https:\/\/doi.org\/10.1586\/14787210.5.5.893.","journal-title":"Expert Rev Anti Infect Ther"},{"key":"934_CR23","doi-asserted-by":"publisher","first-page":"50","DOI":"10.1016\/j.nmni.2017.05.002","volume":"18","author":"T Weitzel","year":"2017","unstructured":"Weitzel T, Cabrera J, Rosas R, et al. Enteric multiplex PCR panels: a new diagnostic tool for amoebic liver abscess? New Microbes New Infect. 2017;18:50\u20133. https:\/\/doi.org\/10.1016\/j.nmni.2017.05.002.","journal-title":"New Microbes New Infect"},{"key":"934_CR24","doi-asserted-by":"publisher","unstructured":"Callej\u00f3n Fern\u00e1ndez M, Kohan R, L\u00f3pez Lirola AM, Lecuona Fern\u00e1ndez M. Absceso hep\u00e1tico amebiano en paciente procedente de Gambia [Amoebic liver abscess in a patient from Gambia]. Rev Esp Quimioter. 2023;36(2):214\u20139. https:\/\/doi.org\/10.37201\/req\/106.2022.","DOI":"10.37201\/req\/106.2022"},{"issue":"6","key":"934_CR25","doi-asserted-by":"publisher","first-page":"725","DOI":"10.1016\/j.pathol.2020.06.014","volume":"52","author":"NM Douglas","year":"2020","unstructured":"Douglas NM, Baird RW, Currie BJ. Use of a rapid faeces multiplex PCR assay for diagnosis of amoebic liver abscess. Pathology (Phila). 2020;52(6):725\u20137. https:\/\/doi.org\/10.1016\/j.pathol.2020.06.014.","journal-title":"Pathology (Phila)"}],"container-title":["Infectious Diseases and Therapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s40121-024-00934-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s40121-024-00934-1\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s40121-024-00934-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,27]],"date-time":"2024-03-27T06:07:18Z","timestamp":1711519638000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s40121-024-00934-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3]]},"references-count":25,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2024,3]]}},"alternative-id":["934"],"URL":"https:\/\/doi.org\/10.1007\/s40121-024-00934-1","relation":{},"ISSN":["2193-8229","2193-6382"],"issn-type":[{"value":"2193-8229","type":"print"},{"value":"2193-6382","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3]]},"assertion":[{"value":"10 December 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 January 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 March 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The patient was aware of the conduct and publication of this case study and provided verbal and written informed consent. The study was carried out in accordance with the principles of the Helsinki Declaration of 1964 and its later amendments. Ethics committee approval was not required for publication of this case study.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical Approval"}},{"value":"The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of Interest"}}]}}