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It is responsible for maintenance of pregnancy in mares during early gestation and fairly valuable for inducing superovulation in animals other than equines. The alpha subunit is common, while beta subunit is species-specific in all glycoprotein hormones. In the present investigation, molecular cloning and in silico characterization including homology modeling and molecular docking analysis of the equine chorionic gonadotropin (eCG) alpha subunit was carried out for gaining structural and functional insights into the eCG alpha subunit and its possible interaction with ganirelix, a gonadotropin-releasing hormone (GnRH) antagonist. The equine chorionic gonadotropin (eCG) alpha subunit expressed in pituitary gland was selected, amplified from total RNA, cloned and sequenced. The in silico analyses were made for homology modelling, structural details, epitope identification and chromosomal localization. Molecular docking studies of eCG alpha were undertaken with a drug ganirelix which is used to control ovulation and has antagonistic activity against GnRH. The protein sequence corresponding to selected open reading frame (ORF) was 99\u2013100% similar with domesticated horse, Przewalski\u2019s horse, and 92\u201393% with Burchell\u2019s zebra and donkey. Molecular docking studies revealed the possible interaction of eCG alpha with ganirelix. The possible drug-macromolecule interactions were visualized between eCG alpha and ganirelix. The study will provide structural insight into unique sites and an alternate route of gonadotropin suppression applicable to assisted reproductive technologies.<\/jats:p>","DOI":"10.1007\/s40203-017-0025-1","type":"journal-article","created":{"date-parts":[[2017,7,18]],"date-time":"2017-07-18T10:16:50Z","timestamp":1500373010000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Molecular characterization, modeling, in silico analysis of equine pituitary gonadotropin alpha subunit and docking interaction studies with ganirelix"],"prefix":"10.1007","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1148-3346","authenticated-orcid":false,"given":"Anuradha","family":"Bhardwaj","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1740-2009","authenticated-orcid":false,"given":"Varij","family":"Nayan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Parvati","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanjay","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yash","family":"Pal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jitender","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,7,18]]},"reference":[{"issue":"11","key":"25_CR1","doi-asserted-by":"publisher","first-page":"785","DOI":"10.1111\/jne.12178","volume":"26","author":"MH Abel","year":"2014","unstructured":"Abel MH, Widen A, Wang X, Huhtaniemi I, Pakarinen P, Kumar TR, Christian HC (2014) Pituitary gonadotrophic hormone synthesis, secretion, subunit gene expression and cell structure in normal and follicle-stimulating hormone \u03b2 knockout, follicle-stimulating hormone receptor knockout, luteinising hormone receptor knockout, hypogonadal and ovariectomised female mice. 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All the procedures for sample collection were performed according to the approval by the Institute Animal Ethics Committee (IAEC) of ICAR-National Research Centre on Equines vide NRCE\/CPCSEA\/2012-13\/dated the 18th July 2012.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Statement of ethics approval"}},{"value":"We give our consent to participate under the \u2018Ethics, consent and permissions\u2019 heading.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to participate"}},{"value":"The data generated during this study and supporting this study are included in this article and also in additional files within the Additional file 1: Figure S1\u2013S11 and Additional file 2: tables S1-S2. The dataset analyzed during the current study is available from the corresponding author on a reasonable request.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Availability of data and materials"}},{"value":"None of the authors have any competing interests in the manuscript.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"This work was financially supported by ICAR-National Research Centre on Equines, Hisar (URL: ) under the institute project (code: IXX02769).","order":6,"name":"Ethics","group":{"name":"EthicsHeading","label":"Funding"}}],"article-number":"5"}}