{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T13:32:16Z","timestamp":1782739936921,"version":"3.54.5"},"reference-count":24,"publisher":"Springer Science and Business Media LLC","license":[{"start":{"date-parts":[[2021,6,16]],"date-time":"2021-06-16T00:00:00Z","timestamp":1623801600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0"},{"start":{"date-parts":[[2021,6,16]],"date-time":"2021-06-16T00:00:00Z","timestamp":1623801600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"funder":[{"name":"Guangzhou Science and Technology Planning Program","award":["202002030023"],"award-info":[{"award-number":["202002030023"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Diagn Ther"],"DOI":"10.1007\/s40291-021-00532-8","type":"journal-article","created":{"date-parts":[[2021,6,16]],"date-time":"2021-06-16T11:02:41Z","timestamp":1623841361000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Detecting ALK Rearrangement with RT-PCR: A Reliable Approach Compared with Next-Generation Sequencing in Patients with NSCLC"],"prefix":"10.1007","author":[{"given":"Yukun","family":"Kuang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peihang","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiyu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifan","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zimu","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"RunJing","family":"Gan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huixia","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yubiao","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Yao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changbin","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zunfu","family":"Ke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kejing","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,6,16]]},"reference":[{"issue":"23","key":"532_CR1","doi-asserted-by":"publisher","first-page":"2167","DOI":"10.1056\/NEJMoa1408440","volume":"371","author":"BJ Solomon","year":"2014","unstructured":"Solomon BJ, Mok T, Kim DW, Wu YL, Nakagawa K, Mekhail T, et al. First-line crizotinib versus chemotherapy in ALK-positive lung cancer. N Engl J Med. 2014;371(23):2167\u201377.","journal-title":"N Engl J Med."},{"key":"532_CR2","doi-asserted-by":"publisher","first-page":"154","DOI":"10.1016\/j.lungcan.2020.09.012","volume":"149","author":"H Tao","year":"2020","unstructured":"Tao H, Shi L, Zhou A, Li H, Gai F, Huang Z, et al. Distribution of EML4-ALK fusion variants and clinical outcomes in patients with resected non-small cell lung cancer. Lung Cancer. 2020;149:154\u201361.","journal-title":"Lung Cancer"},{"issue":"26","key":"532_CR3","doi-asserted-by":"publisher","first-page":"2537","DOI":"10.1056\/NEJMc1404894","volume":"370","author":"AT Shaw","year":"2014","unstructured":"Shaw AT, Engelman JA. Ceritinib in ALK-rearranged non-small-cell lung cancer. N Engl J Med. 2014;370(26):2537\u20139.","journal-title":"N Engl J Med."},{"issue":"7153","key":"532_CR4","doi-asserted-by":"publisher","first-page":"561","DOI":"10.1038\/nature05945","volume":"448","author":"M Soda","year":"2007","unstructured":"Soda M, Choi YL, Enomoto M, et al. Identification of the transforming EML4-ALK fusion gene in non-small-cell lung cancer. Nature. 2007;448(7153):561\u20136.","journal-title":"Nature"},{"issue":"4","key":"532_CR5","doi-asserted-by":"publisher","first-page":"611","DOI":"10.1097\/JTO.0000000000000465","volume":"10","author":"TM Rogers","year":"2015","unstructured":"Rogers TM, Russell PA, Wright G, et al. Comparison of methods in the detection of ALK and ROS1 rearrangements in lung cancer. J Thorac Oncol. 2015;10(4):611\u20138.","journal-title":"J Thorac Oncol."},{"issue":"2","key":"532_CR6","first-page":"70","volume":"3","author":"C Teixid\u00f3","year":"2014","unstructured":"Teixid\u00f3 C, Karachaliou N, Peg V, Gimenez-Capitan A, Rosell R. Concordance of IHC, FISH and RT-PCR for EML4-ALK rearrangements. Transl Lung Cancer Res. 2014;3(2):70\u20134.","journal-title":"Transl Lung Cancer Res."},{"key":"532_CR7","doi-asserted-by":"publisher","first-page":"e70839","DOI":"10.1371\/journal.pone.0070839","volume":"8","author":"YC Wu","year":"2013","unstructured":"Wu YC, Chang IC, Wang CL, et al. Comparison of IHC, FISH and RT-PCR methods for detection of ALK rearrangements in 312 non-small cell lung cancer patients in Taiwan. PLoS ONE. 2013;8:e70839.","journal-title":"PLoS ONE"},{"issue":"6","key":"532_CR8","doi-asserted-by":"publisher","first-page":"1525","DOI":"10.7150\/jca.36580","volume":"11","author":"S Lu","year":"2020","unstructured":"Lu S, Lu C, Xiao Y, et al. Comparison of EML4-ALK fusion gene positive rate in different detection methods and samples of non-small cell lung cancer. J Cancer. 2020;11(6):1525\u201331.","journal-title":"J Cancer."},{"key":"532_CR9","doi-asserted-by":"publisher","first-page":"1546","DOI":"10.1097\/JTO.0000000000000668","volume":"10","author":"Y Wang","year":"2015","unstructured":"Wang Y, Zhang J, Gao G, et al. EML4-ALK fusion detected by RT-PCR confers similar response to crizotinib as detected by FISH in patients with advanced non-small-cell lung cancer. J Thorac Oncol. 2015;10:1546\u201352.","journal-title":"J Thorac Oncol"},{"key":"532_CR10","doi-asserted-by":"publisher","first-page":"308","DOI":"10.3390\/ijms18020308","volume":"18","author":"R Kamps","year":"2017","unstructured":"Kamps R, Brand\u00e3o RD, Bosch BJ, et al. Next-generation sequencing in oncology: genetic diagnosis, risk prediction and cancer classification. Int J Mol Sci. 2017;18:308.","journal-title":"Int J Mol Sci"},{"issue":"3","key":"532_CR11","doi-asserted-by":"publisher","first-page":"413","DOI":"10.1016\/j.jtho.2017.11.117","volume":"13","author":"I Letovanec","year":"2018","unstructured":"Letovanec I, Finn S, Zygoura P, et al. Evaluation of NGS and RT-PCR methods for ALK rearrangement in European NSCLC patients: results from the European Thoracic Oncology Platform Lungscape Project. J Thorac Oncol. 2018;13(3):413\u201325.","journal-title":"J Thorac Oncol."},{"key":"532_CR12","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1016\/j.jmoldx.2016.10.007","volume":"19","author":"CJ Lih","year":"2017","unstructured":"Lih CJ, Harrington RD, Sims DJ, Harper KN, Bouk CH, Datta V, Yau J, Singh RR, Routbort MJ, Luthra R, et al. Analytical validation of the next-generation sequencing assay for a nationwide signal-finding clinical trial: molecular analysis for Therapy Choice Clinical Trial. J Mol Diagn. 2017;19:313\u201327.","journal-title":"J Mol Diagn"},{"key":"532_CR13","doi-asserted-by":"publisher","first-page":"1809","DOI":"10.2147\/DDDT.S196189","volume":"13","author":"J Liu","year":"2019","unstructured":"Liu J, Mu Z, Liu L, Li K, Jiang R, Chen P, Zhou Q, Jin M, Ma Y, Xie Y, et al. Frequency, clinical features and differential response to therapy of concurrent ALK\/EGFR alterations in Chinese lung cancer patients. Drug Des Devel Ther. 2019;13:1809\u201317.","journal-title":"Drug Des Devel Ther"},{"key":"532_CR14","doi-asserted-by":"publisher","first-page":"1101","DOI":"10.1038\/nprot.2008.73","volume":"3","author":"TD Schmittgen","year":"2008","unstructured":"Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative C(T) method. Nat Protoc. 2008;3:1101\u20138.","journal-title":"Nat Protoc"},{"key":"532_CR15","doi-asserted-by":"publisher","first-page":"1111","DOI":"10.3389\/fimmu.2020.01111","volume":"11","author":"WI Lee","year":"2020","unstructured":"Lee WI, Huang JL, Lin SJ, et al. Lower T regulatory and Th17 cell populations predicted by RT-PCR-amplified FOXP3 and ROR\u03b3t genes are not rare in patients with primary immunodeficiency diseases. Front Immunol. 2020;11:1111.","journal-title":"Front Immunol"},{"issue":"11","key":"532_CR16","doi-asserted-by":"publisher","first-page":"e1070","DOI":"10.1634\/theoncologist.2018-0572","volume":"24","author":"S Wen","year":"2019","unstructured":"Wen S, Dai L, Wang L, et al. Genomic signature of driver genes identified by target next-generation sequencing in Chinese non-small cell lung cancer. Oncologist. 2019;24(11):e1070\u201381.","journal-title":"Oncologist."},{"issue":"7","key":"532_CR17","doi-asserted-by":"publisher","first-page":"1223","DOI":"10.1016\/j.jtho.2020.02.023","volume":"15","author":"W Li","year":"2020","unstructured":"Li W, Liu Y, Li W, Chen L, Ying J. Intergenic breakpoints identified by DNA sequencing confound targetable kinase fusion detection in NSCLC. J Thorac Oncol. 2020;15(7):1223\u201331.","journal-title":"J Thorac Oncol."},{"issue":"4","key":"532_CR18","doi-asserted-by":"publisher","first-page":"737","DOI":"10.1016\/j.jtho.2018.12.020","volume":"14","author":"KD Davies","year":"2019","unstructured":"Davies KD, Lomboy A, Lawrence CA, et al. DNA-based versus RNA-based detection of MET Exon 14 skipping events in lung cancer. J Thorac Oncol. 2019;14(4):737\u201341.","journal-title":"J Thorac Oncol."},{"issue":"15","key":"532_CR19","doi-asserted-by":"publisher","first-page":"4712","DOI":"10.1158\/1078-0432.CCR-19-0225","volume":"25","author":"R Benayed","year":"2019","unstructured":"Benayed R, Offin M, Mullaney K, et al. High yield of RNA sequencing for targetable kinase fusions in lung adenocarcinomas with no mitogenic driver alteration detected by DNA sequencing and low tumor mutation burden. Clin Cancer Res. 2019;25(15):4712\u201322.","journal-title":"Clin Cancer Res."},{"issue":"1","key":"532_CR20","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.lungcan.2011.10.017","volume":"76","author":"E Thunnissen","year":"2012","unstructured":"Thunnissen E, Kerr KM, Herth FJ, et al. The challenge of NSCLC diagnosis and predictive analysis on small samples. Practical approach of a working group. Lung Cancer. 2012;76(1):1\u201318.","journal-title":"Lung Cancer"},{"issue":"2","key":"532_CR21","doi-asserted-by":"publisher","first-page":"168","DOI":"10.1038\/modpathol.2013.134","volume":"27","author":"AJ Smits","year":"2014","unstructured":"Smits AJ, Kummer JA, de Bruin PC, et al. The estimation of tumor cell percentage for molecular testing by pathologists is not accurate. Mod Pathol. 2014;27(2):168\u201374.","journal-title":"Mod Pathol."},{"issue":"2","key":"532_CR22","doi-asserted-by":"publisher","first-page":"250","DOI":"10.1016\/j.jtho.2020.10.008","volume":"16","author":"MM Zheng","year":"2021","unstructured":"Zheng MM, Li YS, Tu HY, et al. Genotyping of cerebrospinal fluid associated with osimertinib response and resistance for leptomeningeal metastases in EGFR-mutated NSCLC. J Thorac Oncol. 2021;16(2):250\u20138.","journal-title":"J Thorac Oncol."},{"key":"532_CR23","doi-asserted-by":"publisher","first-page":"3578748","DOI":"10.1155\/2020\/3578748","volume":"2020","author":"DS Heriyanto","year":"2020","unstructured":"Heriyanto DS, Trisnawati I, Kumara EG, et al. The prevalence of the EML4-ALK fusion gene in cytology specimens from patients with lung adenocarcinoma. Pulm Med. 2020;2020:3578748.","journal-title":"Pulm Med."},{"key":"532_CR24","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1016\/j.lungcan.2016.01.014","volume":"94","author":"Y Wang","year":"2016","unstructured":"Wang Y, Liu Y, Zhao C, et al. Feasibility of cytological specimens for ALK fusion detection in patients with advanced NSCLC using the method of RT-PCR. Lung Cancer. 2016;94:28\u201334.","journal-title":"Lung Cancer"}],"container-title":["Molecular Diagnosis & Therapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s40291-021-00532-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s40291-021-00532-8\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s40291-021-00532-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,6,16]],"date-time":"2021-06-16T11:30:39Z","timestamp":1623843039000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s40291-021-00532-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,16]]},"references-count":24,"alternative-id":["532"],"URL":"https:\/\/doi.org\/10.1007\/s40291-021-00532-8","relation":{},"ISSN":["1177-1062","1179-2000"],"issn-type":[{"value":"1177-1062","type":"print"},{"value":"1179-2000","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,16]]},"assertion":[{"value":"28 April 2021","order":1,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 June 2021","order":2,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This work and the open access were supported by a grant from Guangzhou Science and Technology Planning Program (202002030023) and Natural Science Foundation of Guangdong Province (18zxxt17).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Funding"}},{"value":"Fei Yao and Changbin Zhu are employed by Amoy Diagnostics Co., Ltd. Yukun Kuang, Peihang Xu, Jiyu Wang, Yifan Zheng, Xue Sun, Zimu Li, RunJing Gan, Huixia Li, Yubiao Guo, Zunfu Ke, Kejing Tang have no conflicts of interest that are directly relevant to the content of this article.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"This study was approved by the institutional review board of the First Affiliated Hospital, Sun Yat-sen University (no. Lunshen[2013]C-084).","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"All patients provided written informed consent before enrollment; participation in this study was covered by this protocol.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent"}},{"value":"KT, ZK, YK, LB, PX, JW, and YZ designed the study and wrote the paper. XS, ZL, RG, HL, and YG performed the study by collecting cases with clinical data and performing the sample mutation test. FY and CZ analyzed the data and wrote the paper.","order":6,"name":"Ethics","group":{"name":"EthicsHeading","label":"Author contributions"}},{"value":"The data that support the findings of this study are available on request from the corresponding author.","order":7,"name":"Ethics","group":{"name":"EthicsHeading","label":"Data availability statement"}}]}}