{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T20:38:37Z","timestamp":1793047117937,"version":"4.5.0"},"reference-count":43,"publisher":"Springer Science and Business Media LLC","issue":"6","license":[{"start":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T00:00:00Z","timestamp":1786147200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0"},{"start":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T00:00:00Z","timestamp":1786147200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"funder":[{"name":"Alma Mater Studiorum - Universit\u00e0 di Bologna","id":[{"id":"https:\/\/ror.org\/01111rn36","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Diagn Ther"],"published-print":{"date-parts":[[2026,11]]},"DOI":"10.1007\/s40291-026-00866-1","type":"journal-article","created":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T14:33:09Z","timestamp":1786199589000},"page":"865-874","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Non-Invasive Prenatal Testing in Donor-Oocyte Pregnancies: Biological Determinants, Algorithmic Limitations and Clinical Implications"],"prefix":"10.1007","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5001-8271","authenticated-orcid":false,"given":"Michele","family":"Miscia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irene","family":"Pesaresi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benedetta","family":"Dini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danila","family":"Morano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo Ivo","family":"Cavoretto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Farina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,8,8]]},"reference":[{"issue":"9076","key":"866_CR1","doi-asserted-by":"publisher","first-page":"485","DOI":"10.1016\/S0140-6736(97)02174-0","volume":"350","author":"YM Lo","year":"1997","unstructured":"Lo YM, Corbetta N, Chamberlain PF, Rai V, Sargent IL, Redman CW, et al. Presence of fetal DNA in maternal plasma and serum. Lancet. 1997;350(9076):485\u20137. https:\/\/doi.org\/10.1016\/S0140-6736(97)02174-0.","journal-title":"Lancet"},{"issue":"1","key":"866_CR2","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1002\/uog.12513","volume":"42","author":"P Benn","year":"2013","unstructured":"Benn P, Cuckle H, Pergament E. Non-invasive prenatal testing for aneuploidy: current status and future prospects. Ultrasound Obstet Gynecol. 2013;42(1):15\u201333. https:\/\/doi.org\/10.1002\/uog.12513.","journal-title":"Ultrasound Obstet Gynecol"},{"issue":"6","key":"866_CR3","doi-asserted-by":"publisher","first-page":"575","DOI":"10.1002\/pd.4103","volume":"33","author":"KH Nicolaides","year":"2013","unstructured":"Nicolaides KH, Syngelaki A, Gil M, Atanasova V, Markova D. Validation of targeted sequencing of single-nucleotide polymorphisms for non-invasive prenatal detection of aneuploidy of chromosomes 13, 18, 21, X, and Y. Prenat Diagn. 2013;33(6):575\u20139. https:\/\/doi.org\/10.1002\/pd.4103.","journal-title":"Prenat Diagn"},{"issue":"2 Pt 1","key":"866_CR4","doi-asserted-by":"publisher","first-page":"210","DOI":"10.1097\/AOG.0000000000000363","volume":"124","author":"E Pergament","year":"2014","unstructured":"Pergament E, Cuckle H, Zimmermann B, Banjevic M, Sigurjonsson S, Ryan A, et al. Single-nucleotide polymorphism-based noninvasive prenatal screening in a high-risk and low-risk cohort. Obstet Gynecol. 2014;124(2 Pt 1):210\u20138. https:\/\/doi.org\/10.1097\/AOG.0000000000000363.","journal-title":"Obstet Gynecol"},{"issue":"3","key":"866_CR5","doi-asserted-by":"publisher","first-page":"302","DOI":"10.1002\/uog.17484","volume":"50","author":"MM Gil","year":"2017","unstructured":"Gil MM, Accurti V, Santacruz B, Plana MN, Nicolaides KH. Analysis of cell-free DNA in maternal blood in screening for aneuploidies: updated meta-analysis. Ultrasound Obstet Gynecol. 2017;50(3):302\u201314. https:\/\/doi.org\/10.1002\/uog.17484.","journal-title":"Ultrasound Obstet Gynecol"},{"key":"866_CR6","doi-asserted-by":"publisher","unstructured":"American College of Obstetricians and Gynecologists\u2019 Committee on Practice Bulletins\u2014Obstetrics, Committee on Genetics, Society for Maternal-Fetal Medicine. Screening for fetal chromosomal abnormalities: ACOG practice bulletin, number 226. Obstet Gynecol. 2020;136(4):e48\u2013e69. https:\/\/doi.org\/10.1097\/AOG.0000000000004084","DOI":"10.1097\/AOG.0000000000004084"},{"issue":"5","key":"866_CR7","doi-asserted-by":"publisher","first-page":"464","DOI":"10.1056\/NEJMra1705345","volume":"379","author":"DW Bianchi","year":"2018","unstructured":"Bianchi DW, Chiu RWK. Sequencing of circulating cell-free DNA during pregnancy. N Engl J Med. 2018;379(5):464\u201373. https:\/\/doi.org\/10.1056\/NEJMra1705345.","journal-title":"N Engl J Med"},{"issue":"2","key":"866_CR8","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1002\/pd.5620","volume":"40","author":"L Hui","year":"2020","unstructured":"Hui L, Bianchi DW. Fetal fraction and noninvasive prenatal testing: what clinicians need to know. Prenat Diagn. 2020;40(2):155\u201363. https:\/\/doi.org\/10.1002\/pd.5620.","journal-title":"Prenat Diagn"},{"key":"866_CR9","doi-asserted-by":"publisher","first-page":"1338516","DOI":"10.3389\/fmed.2024.1338516","volume":"11","author":"J Caradeux","year":"2024","unstructured":"Caradeux J, Fern\u00e1ndez B, \u00c1vila F, Valenzuela A, Mondi\u00f3n M, Figueras F. Pregnancies through oocyte donation. A mini review of pathways involved in placental dysfunction. Front Med (Lausanne). 2024;11:1338516. https:\/\/doi.org\/10.3389\/fmed.2024.1338516.","journal-title":"Front Med (Lausanne)."},{"key":"866_CR10","doi-asserted-by":"publisher","unstructured":"Smeenk J, de Neubourg D, Ansseau C, et al. Assisted reproductive technology (ART) in Europe, 2021 and development of a strategy of vigilance: preliminary results generated from European registers by the ESHRE EIM Consortium. Hum Reprod. 2024;39(Suppl 1):deae108.136. https:\/\/doi.org\/10.1093\/humrep\/deae108.136","DOI":"10.1093\/humrep\/deae108.136"},{"issue":"5","key":"866_CR11","doi-asserted-by":"publisher","first-page":"326","DOI":"10.2174\/1389202923666220518095758","volume":"23","author":"V Kamath","year":"2022","unstructured":"Kamath V, Chacko MP, Kamath MS. Non-invasive prenatal testing in pregnancies following assisted reproduction. Curr Genomics. 2022;23(5):326\u201336. https:\/\/doi.org\/10.2174\/1389202923666220518095758.","journal-title":"Curr Genomics"},{"issue":"3","key":"866_CR12","doi-asserted-by":"publisher","first-page":"328","DOI":"10.1016\/j.ajog.2015.11.020","volume":"214","author":"P Masoudian","year":"2016","unstructured":"Masoudian P, Nasr A, de Nanassy J, Fung-Kee-Fung K, Bainbridge SA, El Demellawy D. Oocyte donation pregnancies and the risk of preeclampsia or gestational hypertension: a systematic review and metaanalysis. Am J Obstet Gynecol. 2016;214(3):328\u201339. https:\/\/doi.org\/10.1016\/j.ajog.2015.11.020.","journal-title":"Am J Obstet Gynecol"},{"issue":"3","key":"866_CR13","doi-asserted-by":"publisher","first-page":"586","DOI":"10.1093\/humrep\/deab267","volume":"37","author":"A Keukens","year":"2022","unstructured":"Keukens A, van Wely M, van der Meulen C, Mochtar MH. Pre-eclampsia in pregnancies resulting from oocyte donation, natural conception or IVF: a systematic review and meta-analysis. Hum Reprod. 2022;37(3):586\u201399. https:\/\/doi.org\/10.1093\/humrep\/deab267.","journal-title":"Hum Reprod"},{"issue":"4","key":"866_CR14","doi-asserted-by":"publisher","first-page":"572","DOI":"10.1093\/humrep\/dey033","volume":"33","author":"TJ Lee","year":"2018","unstructured":"Lee TJ, Rolnik DL, Menezes MA, McLennan AC, da Silva Costa F. Cell-free fetal DNA testing in singleton IVF conceptions. Hum Reprod. 2018;33(4):572\u20138. https:\/\/doi.org\/10.1093\/humrep\/dey033.","journal-title":"Hum Reprod"},{"issue":"3 Suppl","key":"866_CR15","doi-asserted-by":"publisher","first-page":"e120","DOI":"10.1016\/j.fertnstert.2019.07.434","volume":"112","author":"MK Maisenbacher","year":"2019","unstructured":"Maisenbacher MK, Goldring G, DiNonno W, Ryan A. Donor oocyte pregnancies and fetal fraction: managing patient expectations and providing accurate information. Fertil Steril. 2019;112(3 Suppl):e120\u20131.","journal-title":"Fertil Steril"},{"issue":"1","key":"866_CR16","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajogmf.2022.100752","volume":"5","author":"F Gu","year":"2023","unstructured":"Gu F, Wei Y, Wang X, et al. Programmed frozen embryo transfer cycle increased risk of hypertensive disorders of pregnancy: a multicenter cohort study in ovulatory women. Am J Obstet Gynecol MFM. 2023;5(1):100752. https:\/\/doi.org\/10.1016\/j.ajogmf.2022.100752.","journal-title":"Am J Obstet Gynecol MFM."},{"issue":"5","key":"866_CR17","doi-asserted-by":"publisher","first-page":"482","DOI":"10.1002\/pd.5036","volume":"37","author":"CG Artieri","year":"2017","unstructured":"Artieri CG, Haverty C, Evans EA, Goldberg JD, Haque IS. Noninvasive prenatal screening at low fetal fraction: comparing whole-genome sequencing and single-nucleotide polymorphism methods. Prenat Diagn. 2017;37(5):482\u201390. https:\/\/doi.org\/10.1002\/pd.5036.","journal-title":"Prenat Diagn"},{"issue":"6","key":"866_CR18","doi-asserted-by":"publisher","first-page":"744","DOI":"10.1111\/aogs.13848","volume":"99","author":"LA Juul","year":"2020","unstructured":"Juul LA, Hartwig TS, Ambye L, S\u00f8rensen S, J\u00f8rgensen FS. Noninvasive prenatal testing and maternal obesity: a review. Acta Obstet Gynecol Scand. 2020;99(6):744\u201350. https:\/\/doi.org\/10.1111\/aogs.13848.","journal-title":"Acta Obstet Gynecol Scand"},{"issue":"1","key":"866_CR19","doi-asserted-by":"publisher","first-page":"62","DOI":"10.1186\/s40246-019-0244-0","volume":"13","author":"Y Hou","year":"2019","unstructured":"Hou Y, Yang J, Qi Y, Guo F, Peng H, Wang D, et al. Factors affecting cell-free DNA fetal fraction: statistical analysis of 13,661 maternal plasmas for non-invasive prenatal screening. Hum Genomics. 2019;13(1):62. https:\/\/doi.org\/10.1186\/s40246-019-0244-0.","journal-title":"Hum Genomics"},{"issue":"3","key":"866_CR20","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0280858","volume":"19","author":"J Gazdarica","year":"2024","unstructured":"Gazdarica J, Forgacova N, Sladecek T, Kucharik M, Budis J, Hyblova M, et al. Insights into non-informative results from non-invasive prenatal screening through gestational age, maternal BMI, and age analyses. PLoS ONE. 2024;19(3):e0280858. https:\/\/doi.org\/10.1371\/journal.pone.0280858.","journal-title":"PLoS ONE"},{"issue":"6","key":"866_CR21","doi-asserted-by":"publisher","first-page":"722","DOI":"10.1002\/uog.18993","volume":"52","author":"DL Rolnik","year":"2018","unstructured":"Rolnik DL, da Silva Costa F, Lee TJ, Schmid M, McLennan AC. Association between fetal fraction on cell-free DNA testing and first-trimester markers for pre-eclampsia. Ultrasound Obstet Gynecol. 2018;52(6):722\u20137. https:\/\/doi.org\/10.1002\/uog.18993.","journal-title":"Ultrasound Obstet Gynecol"},{"issue":"10","key":"866_CR22","doi-asserted-by":"publisher","first-page":"1287","DOI":"10.1002\/pd.6028","volume":"41","author":"PG Scheffer","year":"2021","unstructured":"Scheffer PG, Wirjosoekarto SAM, Becking EC, Weiss MM, Bax CJ, Oepkes D, et al. Association between low fetal fraction in cell-free DNA testing and adverse pregnancy outcome: a systematic review. Prenat Diagn. 2021;41(10):1287\u201395. https:\/\/doi.org\/10.1002\/pd.6028.","journal-title":"Prenat Diagn"},{"issue":"10","key":"866_CR23","doi-asserted-by":"publisher","first-page":"1296","DOI":"10.1002\/pd.6034","volume":"41","author":"EC Becking","year":"2021","unstructured":"Becking EC, Wirjosoekarto SAM, Scheffer PG, Huiskes JVM, Remmelink MJ, Sistermans EA, et al. Low fetal fraction in cell-free DNA testing is associated with adverse pregnancy outcome: analysis of a subcohort of the TRIDENT-2 study. Prenat Diagn. 2021;41(10):1296\u2013304. https:\/\/doi.org\/10.1002\/pd.6034.","journal-title":"Prenat Diagn"},{"issue":"10","key":"866_CR24","doi-asserted-by":"publisher","first-page":"1333","DOI":"10.1002\/pd.6423","volume":"43","author":"L Lannoo","year":"2023","unstructured":"Lannoo L, Van Camp J, Brison N, Parijs I, Vancoillie L, Van Den Bogaert K, et al. What helps define outcomes in persistent uninterpretable non-invasive prenatal testing: maternal factors, fetal fraction or quality scores? Prenat Diagn. 2023;43(10):1333\u201343. https:\/\/doi.org\/10.1002\/pd.6423.","journal-title":"Prenat Diagn"},{"issue":"5","key":"866_CR25","doi-asserted-by":"publisher","first-page":"581","DOI":"10.1002\/pd.6778","volume":"45","author":"L Lannoo","year":"2025","unstructured":"Lannoo L, Van Den Bogaert K, Belmans A, Brison N, Dehaspe L, De Langhe E, et al. Persistent uninterpretable or failed prenatal cell-free DNA screening indicates a high-risk pregnancy and is associated with biological factors interfering with cfDNA-analysis: a prospective cohort study. Prenat Diagn. 2025;45(5):581\u201390. https:\/\/doi.org\/10.1002\/pd.6778.","journal-title":"Prenat Diagn"},{"issue":"11","key":"866_CR26","doi-asserted-by":"publisher","first-page":"1417","DOI":"10.1002\/pd.6852","volume":"45","author":"Y Ren","year":"2025","unstructured":"Ren Y, Hao N, Chang J, Jiang Y, Qi Q, Zhou X, et al. Clinical implications of noninvasive prenatal testing failures due to low fetal fraction: associations with adverse maternal and fetal outcomes. Prenat Diagn. 2025;45(11):1417\u201325. https:\/\/doi.org\/10.1002\/pd.6852.","journal-title":"Prenat Diagn"},{"issue":"4","key":"866_CR27","doi-asserted-by":"publisher","first-page":"409","DOI":"10.1007\/s40291-020-00468-5","volume":"24","author":"IF Carbone","year":"2020","unstructured":"Carbone IF, Conforti A, Picarelli S, Morano D, Alviggi C, Farina A. Circulating nucleic acids in maternal plasma and serum in pregnancy complications: are they really useful in clinical practice? A systematic review. Mol Diagn Ther. 2020;24(4):409\u201331. https:\/\/doi.org\/10.1007\/s40291-020-00468-5.","journal-title":"Mol Diagn Ther"},{"issue":"5","key":"866_CR28","doi-asserted-by":"publisher","first-page":"613","DOI":"10.1007\/s40291-018-0353-9","volume":"22","author":"D Morano","year":"2018","unstructured":"Morano D, Rossi S, Lapucci C, Pittalis MC, Farina A. Cell-free DNA (cfDNA) fetal fraction in early- and late-onset fetal growth restriction. Mol Diagn Ther. 2018;22(5):613\u20139. https:\/\/doi.org\/10.1007\/s40291-018-0353-9.","journal-title":"Mol Diagn Ther"},{"issue":"6","key":"866_CR29","doi-asserted-by":"publisher","first-page":"607","DOI":"10.1007\/s40291-022-00611-4","volume":"26","author":"N Salmeri","year":"2022","unstructured":"Salmeri N, Carbone IF, Cavoretto PI, Farina A, Morano D. Epigenetics beyond fetal growth restriction: a comprehensive overview. Mol Diagn Ther. 2022;26(6):607\u201326. https:\/\/doi.org\/10.1007\/s40291-022-00611-4.","journal-title":"Mol Diagn Ther"},{"issue":"1","key":"866_CR30","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1373\/clinchem.2013.215145","volume":"60","author":"Y Wang","year":"2014","unstructured":"Wang Y, Chen Y, Tian F, Zhang J, Song Z, Wu Y, et al. Maternal mosaicism is a significant contributor to discordant sex chromosomal aneuploidies associated with noninvasive prenatal testing. Clin Chem. 2014;60(1):251\u20139. https:\/\/doi.org\/10.1373\/clinchem.2013.215145.","journal-title":"Clin Chem"},{"issue":"3","key":"866_CR31","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajogmf.2020.100152","volume":"2","author":"KA Martin","year":"2020","unstructured":"Martin KA, Samango-Sprouse C, Kantor V, Dhamankar R, Valenti E, Trefogli MT, et al. Detection of maternal X chromosome abnormalities using single nucleotide polymorphism-based noninvasive prenatal testing. Am J Obstet Gynecol MFM. 2020;2(3):100152. https:\/\/doi.org\/10.1016\/j.ajogmf.2020.100152.","journal-title":"Am J Obstet Gynecol MFM"},{"key":"866_CR32","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2024.117770","volume":"554","author":"X Tang","year":"2024","unstructured":"Tang X, Du Y, Chen M, Zhang Y, Wang Z, Zhang F, et al. Relationships among maternal monosomy X mosaicism, maternal trisomy, and discordant sex chromosome aneuploidies. Clin Chim Acta. 2024;554:117770. https:\/\/doi.org\/10.1016\/j.cca.2024.117770.","journal-title":"Clin Chim Acta"},{"issue":"4","key":"866_CR33","doi-asserted-by":"publisher","first-page":"381","DOI":"10.1016\/j.ajog.2023.11.1240","volume":"230","author":"Y Raymond","year":"2024","unstructured":"Raymond Y, Fernando S, Menezes M, Mol BW, McLennan A, da Silva Costa F, et al. Placental, maternal, fetal, and technical origins of false-positive cell-free DNA screening results. Am J Obstet Gynecol. 2024;230(4):381\u20139. https:\/\/doi.org\/10.1016\/j.ajog.2023.11.1240.","journal-title":"Am J Obstet Gynecol"},{"issue":"6","key":"866_CR34","doi-asserted-by":"publisher","first-page":"1140","DOI":"10.1016\/j.ajhg.2022.04.018","volume":"109","author":"L van Prooyen Schuurman","year":"2022","unstructured":"van Prooyen Schuurman L, Sistermans EA, Van Opstal D, Henneman L, Bekker MN, Bax CJ, et al. Clinical impact of additional findings detected by genome-wide non-invasive prenatal testing: follow-up results of the TRIDENT-2 study. Am J Hum Genet. 2022;109(6):1140\u201352. https:\/\/doi.org\/10.1016\/j.ajhg.2022.04.018.","journal-title":"Am J Hum Genet"},{"issue":"6","key":"866_CR35","doi-asserted-by":"publisher","first-page":"1091","DOI":"10.1016\/j.ajhg.2019.10.005","volume":"105","author":"KRM van der Meij","year":"2019","unstructured":"van der Meij KRM, Sistermans EA, Macville MVE, Stevens SJC, Bax CJ, Bekker MN, et al. TRIDENT-2: national implementation of genome-wide non-invasive prenatal testing as a first-tier screening test in the Netherlands. Am J Hum Genet. 2019;105(6):1091\u2013101. https:\/\/doi.org\/10.1016\/j.ajhg.2019.10.005.","journal-title":"Am J Hum Genet"},{"issue":"11","key":"866_CR36","doi-asserted-by":"publisher","first-page":"1155","DOI":"10.1002\/pd.4668","volume":"35","author":"S Gr\u00f6mminger","year":"2015","unstructured":"Gr\u00f6mminger S, Erkan S, Sch\u00f6ck U, Stangier K, Bonnet J, Schloo R, et al. The influence of low molecular weight heparin medication on plasma DNA in pregnant women. Prenat Diagn. 2015;35(11):1155\u20137. https:\/\/doi.org\/10.1002\/pd.4668.","journal-title":"Prenat Diagn"},{"issue":"2","key":"866_CR37","doi-asserted-by":"publisher","first-page":"100","DOI":"10.1002\/pd.5408","volume":"39","author":"N Suzumori","year":"2019","unstructured":"Suzumori N, Sekizawa A, Takeda E, Samura O, Sasaki A, Akaishi R, et al. Classification of factors involved in nonreportable results of noninvasive prenatal testing and prediction of success rate of second NIPT. Prenat Diagn. 2019;39(2):100\u20136. https:\/\/doi.org\/10.1002\/pd.5408.","journal-title":"Prenat Diagn"},{"issue":"3","key":"866_CR38","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajogmf.2022.100846","volume":"5","author":"JF Nitsche","year":"2023","unstructured":"Nitsche JF, Lovell D, Stephens N, Conrad S, Bebeau K, Brost BC. The effects of heparin, aspirin, and maternal clinical factors on the rate of nonreportable cell-free DNA results: a retrospective cohort study. Am J Obstet Gynecol MFM. 2023;5(3):100846. https:\/\/doi.org\/10.1016\/j.ajogmf.2022.100846.","journal-title":"Am J Obstet Gynecol MFM"},{"issue":"1","key":"866_CR39","doi-asserted-by":"publisher","first-page":"95.e1","DOI":"10.1016\/j.ajog.2023.07.005","volume":"230","author":"R Shree","year":"2024","unstructured":"Shree R, MacKinnon HJ, Hannan J, Kolarova TR, Reichel J, Lockwood CM. Anticoagulation use is associated with lower fetal fraction and more indeterminate results. Am J Obstet Gynecol. 2024;230(1):95.e1-95.e10. https:\/\/doi.org\/10.1016\/j.ajog.2023.07.005.","journal-title":"Am J Obstet Gynecol"},{"issue":"6","key":"866_CR40","doi-asserted-by":"publisher","first-page":"738","DOI":"10.1002\/uog.70015","volume":"66","author":"JCA van Eekhout","year":"2025","unstructured":"van Eekhout JCA, Bax CJ, van Prooyen Schuurman L, Becking EC, van der Ven AJEM, Van Opstal D, et al. Performance of non-invasive prenatal testing in vanishing-twin and multiple pregnancies: results of TRIDENT-2 study. Ultrasound Obstet Gynecol. 2025;66(6):738\u201346. https:\/\/doi.org\/10.1002\/uog.70015.","journal-title":"Ultrasound Obstet Gynecol"},{"issue":"6538","key":"866_CR41","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaw3616","volume":"372","author":"YMD Lo","year":"2021","unstructured":"Lo YMD, Han DSC, Jiang P, Chiu RWK. Epigenetics, fragmentomics, and topology of cell-free DNA in liquid biopsies. Science. 2021;372(6538):eaaw3616. https:\/\/doi.org\/10.1126\/science.aaw3616.","journal-title":"Science"},{"issue":"40","key":"866_CR42","doi-asserted-by":"publisher","first-page":"E5503","DOI":"10.1073\/pnas.1508736112","volume":"112","author":"K Sun","year":"2015","unstructured":"Sun K, Jiang P, Chan KCA, Wong J, Cheng YKY, Liang RHS, et al. Plasma DNA tissue mapping by genome-wide methylation sequencing for noninvasive prenatal, cancer, and transplantation assessments. Proc Natl Acad Sci U S A. 2015;112(40):E5503\u201312. https:\/\/doi.org\/10.1073\/pnas.1508736112.","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"12","key":"866_CR43","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1011484","volume":"20","author":"N Yuen","year":"2024","unstructured":"Yuen N, Lemaire M, Wilson SL. Cell-free placental DNA: what do we really know? PLoS Genet. 2024;20(12):e1011484. https:\/\/doi.org\/10.1371\/journal.pgen.1011484.","journal-title":"PLoS Genet"}],"container-title":["Molecular Diagnosis &amp; Therapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s40291-026-00866-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s40291-026-00866-1","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s40291-026-00866-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T20:03:50Z","timestamp":1793045030000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s40291-026-00866-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,8]]},"references-count":43,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,11]]}},"alternative-id":["866"],"URL":"https:\/\/doi.org\/10.1007\/s40291-026-00866-1","relation":{},"ISSN":["1177-1062","1179-2000"],"issn-type":[{"value":"1177-1062","type":"print"},{"value":"1179-2000","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,8]]},"assertion":[{"value":"27 March 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"5 July 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 August 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"No funds, grants, institutional support, or other support were received during the preparation of this article, including from the authors\u2019 affiliated institutions.","order":1,"name":"Ethics","label":"Funding","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Conceptualization: Antonio Farina. Writing \u2013 original draft preparation: Michele Miscia, Benedetta Dini, and Irene Pesaresi. Writing \u2013 review and editing: Michele Miscia, Danila Morano, Paolo Ivo\u00a0Cavoretto, and Antonio Farina. Supervision: Antonio Farina. All authors reviewed the manuscript, contributed to the final version, and approved the submitted manuscript.","order":2,"name":"Ethics","label":"Authors\u2019 Contributions","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare that they have no competing interests. Antonio Farina is an Editorial Board member of\n                      Molecular Diagnosis & Therapy\n                      . Antonio Farina was not involved in the selection of peer reviewers for the manuscript nor any of the subsequent editorial decisions.","order":3,"name":"Ethics","label":"Competing Interests","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"No new datasets were generated or analyzed during the current study.","order":4,"name":"Ethics","label":"Data Availability","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Not applicable.","order":5,"name":"Ethics","label":"Consent for Publication","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Not applicable.","order":6,"name":"Ethics","label":"Informed Consent","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Not applicable.","order":7,"name":"Ethics","label":"Ethics Approval","group":{"name":"EthicsHeading","label":"Declarations"}}]}}