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Despite this, the last 5\u00a0years has seen remarkable advances in the molecular biology of<jats:italic>Strongyloides<\/jats:italic>spp. Genome sequences for<jats:italic>S. stercoralis<\/jats:italic>,<jats:italic>S. ratti<\/jats:italic>,<jats:italic>S. venezuelensis<\/jats:italic>,<jats:italic>S. papillosus<\/jats:italic>, and the related parasite<jats:italic>Parastrongyloides trichosuri<\/jats:italic>were created, annotated, and analyzed. These genomic resources, along with a practical transgenesis platform for<jats:italic>Strongyloides<\/jats:italic>spp., aided a major achievement, the advent of targeted mutagenesis via CRISPR\/Cas9 in<jats:italic>S. stercoralis<\/jats:italic>and<jats:italic>S. ratti<\/jats:italic>. The genome sequences have also enabled significant molecular epidemiologic and phylogenetic findings on human strongyloidiasis, including the first genetic evidence of zoonotic transmission of<jats:italic>S. stercoralis<\/jats:italic>between dogs and humans. Studies of molecular signaling pathways identified the nuclear receptor<jats:italic>Ss<\/jats:italic>-DAF-12 as one that can be manipulated in the parasite by exogenous application of its steroid ligands. The chemotherapeutic implications of this were unscored by a study in which a<jats:italic>Ss<\/jats:italic>-DAF-12 ligand suppressed autoinfection by<jats:italic>S. stercoralis<\/jats:italic>in a new murine model of human strongyloidiasis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Summary<\/jats:title><jats:p>Seminal advances in genomics of<jats:italic>Strongyloides<\/jats:italic>spp. have transformed research into strongyloidiasis, facilitating fundamental phylogenetic and epidemiologic studies and aiding the deployment of CRISPR\/Cas9 gene disruption and editing as functional genomic tools in<jats:italic>Strongyloides<\/jats:italic>spp. Studies of<jats:italic>Ss<\/jats:italic>-DAF-12 signaling in<jats:italic>S. stercoralis<\/jats:italic>demonstrated the potential of this pathway as a novel chemotherapeutic target in parasitic nematodes.<\/jats:p><\/jats:sec>","DOI":"10.1007\/s40475-019-00186-x","type":"journal-article","created":{"date-parts":[[2019,9,13]],"date-time":"2019-09-13T07:03:45Z","timestamp":1568358225000},"page":"161-178","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Advances in the Molecular and Cellular Biology of Strongyloides spp."],"prefix":"10.1007","volume":"6","author":[{"given":"Tegegn G.","family":"Jaleta","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James B.","family":"Lok","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,9,13]]},"reference":[{"issue":"1","key":"186_CR1","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1016\/j.idc.2018.10.006","volume":"33","author":"A Krolewiecki","year":"2019","unstructured":"Krolewiecki A, Nutman TB. 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