{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T16:10:44Z","timestamp":1761235844800},"reference-count":29,"publisher":"Springer Science and Business Media LLC","issue":"12","license":[{"start":{"date-parts":[[2019,1,24]],"date-time":"2019-01-24T00:00:00Z","timestamp":1548288000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Aging Clin Exp Res"],"published-print":{"date-parts":[[2019,12]]},"DOI":"10.1007\/s40520-019-01125-6","type":"journal-article","created":{"date-parts":[[2019,1,25]],"date-time":"2019-01-25T00:32:58Z","timestamp":1548376378000},"page":"1817-1825","update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Vitamin B12-fortified toothpaste improves vitamin status in elderly people: a randomized, double-blind, placebo-controlled study"],"prefix":"10.1007","volume":"31","author":[{"given":"Ayman","family":"Zant","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hussain M.","family":"Awwad","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00fcrgen","family":"Geisel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Markus","family":"Keller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rima","family":"Obeid","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2019,1,24]]},"reference":[{"key":"1125_CR1","doi-asserted-by":"publisher","first-page":"606","DOI":"10.1001\/jamapsychiatry.2016.0274","volume":"73","author":"B Hooshmand","year":"2016","unstructured":"Hooshmand B, Mangialasche F, Kalpouzos G et al (2016) Association of vitamin B12, folate, and sulfur amino acids with brain magnetic resonance imaging measures in older adults: a longitudinal population-based study. JAMA Psychiatry 73:606\u2013613","journal-title":"JAMA Psychiatry"},{"key":"1125_CR2","doi-asserted-by":"publisher","first-page":"250","DOI":"10.3945\/ajcn.115.116509","volume":"103","author":"A Brito","year":"2016","unstructured":"Brito A, Verdugo R, Hertrampf E et al (2016) Vitamin B-12 treatment of asymptomatic, deficient, elderly Chileans improves conductivity in myelinated peripheral nerves, but high serum folate impairs vitamin B-12 status response assessed by the combined indicator of vitamin B-12 status. Am J Clin Nutr 103:250\u2013257","journal-title":"Am J Clin Nutr"},{"key":"1125_CR3","doi-asserted-by":"publisher","first-page":"750","DOI":"10.1093\/ajcn\/66.4.750","volume":"66","author":"R Carmel","year":"1997","unstructured":"Carmel R (1997) Cobalamin, the stomach, and aging. Am J Clin Nutr 66:750\u2013759","journal-title":"Am J Clin Nutr"},{"key":"1125_CR4","first-page":"639","volume-title":"Bailli\u00e8re\u2019s clinical haematology. Megaloblastic anaemia","author":"R Carmel","year":"1995","unstructured":"Carmel R (1995) Malabsorption of food-cobalamin. In: Wickramasinghe SN (ed) Bailli\u00e8re\u2019s clinical haematology. Megaloblastic anaemia, vol\u00a08. Bailli\u00e8re Tindall, London, pp\u00a0639\u2013655"},{"key":"1125_CR5","doi-asserted-by":"publisher","first-page":"373","DOI":"10.1093\/jn\/137.2.373","volume":"137","author":"J Blacher","year":"2007","unstructured":"Blacher J, Czernichow S, Raphael M et al (2007) Very low oral doses of vitamin B-12 increase serum concentrations in elderly subjects with food-bound vitamin B-12 malabsorption. J Nutr 137:373\u2013378","journal-title":"J Nutr"},{"key":"1125_CR6","doi-asserted-by":"publisher","first-page":"52","DOI":"10.1093\/ajcn\/83.1.52","volume":"83","author":"MV Bor","year":"2006","unstructured":"Bor MV, Lydeking-Olsen E, Moller J et al (2006) A daily intake of approximately 6 microg vitamin B-12 appears to saturate all the vitamin B-12-related variables in Danish postmenopausal women. Am J Clin Nutr 83:52\u201358","journal-title":"Am J Clin Nutr"},{"key":"1125_CR7","doi-asserted-by":"publisher","first-page":"568","DOI":"10.1093\/ajcn\/82.3.568","volume":"82","author":"RA Dhonukshe-Rutten","year":"2005","unstructured":"Dhonukshe-Rutten RA, van de Groot ZM, Eussen LC et al (2005) Effect of supplementation with cobalamin carried either by a milk product or a capsule in mildly cobalamin-deficient elderly Dutch persons. Am J Clin Nutr 82:568\u2013574","journal-title":"Am J Clin Nutr"},{"key":"1125_CR8","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1080\/01639360903574734","volume":"29","author":"NR Rozgony","year":"2010","unstructured":"Rozgony NR, Fang C, Kuczmarski MF, Bob H (2010) Vitamin B(12) deficiency is linked with long-term use of proton pump inhibitors in institutionalized older adults: could a cyanocobalamin nasal spray be beneficial? J Nutr Elder 29:87\u201399","journal-title":"J Nutr Elder"},{"key":"1125_CR9","doi-asserted-by":"publisher","first-page":"430","DOI":"10.1053\/gast.1997.v113.pm9247460","volume":"113","author":"WB Slot","year":"1997","unstructured":"Slot WB, Merkus FW, van Deventer SJ et al (1997) Normalization of plasma vitamin B12 concentration by intranasal hydroxocobalamin in vitamin B12-deficient patients. Gastroenterology 113:430\u2013433","journal-title":"Gastroenterology"},{"key":"1125_CR10","doi-asserted-by":"publisher","first-page":"618","DOI":"10.3945\/ajcn.116.141978","volume":"105","author":"AK Siebert","year":"2017","unstructured":"Siebert AK, Obeid R, Weder S et al (2017) Vitamin B-12-fortified toothpaste improves vitamin status in vegans: a 12-wk randomized placebo-controlled study. Am J Clin Nutr 105:618\u2013625","journal-title":"Am J Clin Nutr"},{"key":"1125_CR11","doi-asserted-by":"publisher","first-page":"269","DOI":"10.3109\/00365518809167494","volume":"48","author":"E Nexo","year":"1988","unstructured":"Nexo E, Hansen MR, Konradsen L (1988) Human salivary epidermal growth factor, haptocorrin and amylase before and after prolonged exercise. Scand J Clin Lab Invest 48:269\u2013273","journal-title":"Scand J Clin Lab Invest"},{"key":"1125_CR12","doi-asserted-by":"publisher","first-page":"264","DOI":"10.1016\/0304-4165(85)90088-1","volume":"838","author":"E Nexo","year":"1985","unstructured":"Nexo E, Hansen M, Poulsen SS et al (1985) Characterization and immunohistochemical localization of rat salivary cobalamin-binding protein and comparison with human salivary haptocorrin. Biochim Biophys Acta 838:264\u2013269","journal-title":"Biochim Biophys Acta"},{"key":"1125_CR13","doi-asserted-by":"publisher","first-page":"567","DOI":"10.1373\/clinchem.2007.096784","volume":"54","author":"J Brady","year":"2008","unstructured":"Brady J, Wilson L, McGregor L et al (2008) Active B12: a rapid, automated assay for holotranscobalamin on the Abbott AxSYM analyzer. Clin Chem 54:567\u2013573","journal-title":"Clin Chem"},{"key":"1125_CR14","doi-asserted-by":"publisher","first-page":"1225","DOI":"10.1515\/cclm-2014-0818","volume":"53","author":"SN Fedosov","year":"2015","unstructured":"Fedosov SN, Brito A, Miller JW et al (2015) Combined indicator of vitamin B12 status: modification for missing biomarkers and folate status and recommendations for revised cut-points. Clin Chem Lab Med 53:1225","journal-title":"Clin Chem Lab Med"},{"key":"1125_CR15","doi-asserted-by":"crossref","first-page":"1768","DOI":"10.1093\/clinchem\/48.10.1768","volume":"48","author":"E Nexo","year":"2002","unstructured":"Nexo E, Hvas AM, Bleie O et al (2002) Holo-transcobalamin is an early marker of changes in cobalamin homeostasis. A randomized placebo-controlled study. Clin Chem 48:1768\u20131771","journal-title":"Clin Chem"},{"key":"1125_CR16","doi-asserted-by":"publisher","first-page":"2151","DOI":"10.1373\/clinchem.2005.055509","volume":"51","author":"MV Bor","year":"2005","unstructured":"Bor MV, Cetin M, Aytac S et al (2005) Nonradioactive vitamin B12 absorption test evaluated in controls and in patients with inherited malabsorption of vitamin B12. Clin Chem 51:2151\u20132155","journal-title":"Clin Chem"},{"key":"1125_CR17","doi-asserted-by":"publisher","first-page":"432","DOI":"10.1373\/clinchem.2009.131524","volume":"56","author":"TF Hardlei","year":"2010","unstructured":"Hardlei TF, Morkbak AL, Bor MV et al (2010) Assessment of vitamin B(12) absorption based on the accumulation of orally administered cyanocobalamin on transcobalamin. Clin Chem 56:432\u2013436","journal-title":"Clin Chem"},{"key":"1125_CR18","doi-asserted-by":"publisher","first-page":"2119","DOI":"10.3945\/jn.109.107359","volume":"139","author":"DS Bhat","year":"2009","unstructured":"Bhat DS, Thuse NV, Lubree HG et al (2009) Increases in plasma holotranscobalamin can be used to assess vitamin B-12 absorption in individuals with low plasma vitamin B-12. J Nutr 139:2119\u20132123","journal-title":"J Nutr"},{"key":"1125_CR19","doi-asserted-by":"publisher","first-page":"784","DOI":"10.3945\/jn.113.189738","volume":"144","author":"O Midttun","year":"2014","unstructured":"Midttun O, Townsend MK, Nygard O et al (2014) Most blood biomarkers related to vitamin status, one-carbon metabolism, and the kynurenine pathway show adequate preanalytical stability and within-person reproducibility to allow assessment of exposure or nutritional status in healthy women and cardiovascular patients. J Nutr 144:784\u2013790","journal-title":"J Nutr"},{"key":"1125_CR20","doi-asserted-by":"publisher","first-page":"497","DOI":"10.1007\/s00394-012-0351-3","volume":"52","author":"SH Kirsch","year":"2013","unstructured":"Kirsch SH, Herrmann W, Eckert R et al (2013) Factors affecting the distribution of folate forms in the serum of elderly German adults. Eur J Nutr 52:497\u2013504","journal-title":"Eur J Nutr"},{"key":"1125_CR21","doi-asserted-by":"publisher","first-page":"991","DOI":"10.3945\/ajcn.115.119768","volume":"102","author":"JM Bland","year":"2015","unstructured":"Bland JM, Altman DG (2015) Best (but oft forgotten) practices: testing for treatment effects in randomized trials by separate analyses of changes from baseline in each group is a misleading approach. Am J Clin Nutr 102:991\u2013994","journal-title":"Am J Clin Nutr"},{"key":"1125_CR22","doi-asserted-by":"publisher","first-page":"571","DOI":"10.3945\/ajcn.2009.28082","volume":"91","author":"MV Bor","year":"2010","unstructured":"Bor MV, von Castel-Roberts KM, Kauwell GP et al (2010) Daily intake of 4 to 7 microg dietary vitamin B-12 is associated with steady concentrations of vitamin B-12-related biomarkers in a healthy young population. Am J Clin Nutr 91:571\u2013577","journal-title":"Am J Clin Nutr"},{"key":"1125_CR23","doi-asserted-by":"publisher","first-page":"142","DOI":"10.3945\/jn.112.169193","volume":"143","author":"MH Hill","year":"2013","unstructured":"Hill MH, Flatley JE, Barker ME et al (2013) A vitamin B-12 supplement of 500\u00a0\u00b5g\/d for eight weeks does not normalize urinary methylmalonic acid or other biomarkers of vitamin B-12 status in elderly people with moderately poor vitamin B-12 status. J Nutr 143:142\u2013147","journal-title":"J Nutr"},{"key":"1125_CR24","doi-asserted-by":"publisher","first-page":"915","DOI":"10.3945\/jn.113.190256","volume":"144","author":"AJ MacFarlane","year":"2014","unstructured":"MacFarlane AJ, Shi Y, Greene-Finestone LS (2014) High-dose compared with low-dose vitamin B-12 supplement use is not associated with higher vitamin B-12 status in children, adolescents, and older adults. J Nutr 144:915\u2013920","journal-title":"J Nutr"},{"key":"1125_CR25","doi-asserted-by":"publisher","first-page":"390","DOI":"10.3945\/ajcn.112.033951","volume":"97","author":"C Dullemeijer","year":"2013","unstructured":"Dullemeijer C, Souverein OW, Doets EL et al (2013) Systematic review with dose\u2013response meta-analyses between vitamin B-12 intake and European Micronutrient Recommendations Aligned\u2019s prioritized biomarkers of vitamin B-12 including randomized controlled trials and observational studies in adults and elderly persons. Am J Clin Nutr 97:390\u2013402","journal-title":"Am J Clin Nutr"},{"key":"1125_CR26","doi-asserted-by":"publisher","first-page":"1167","DOI":"10.1001\/archinte.165.10.1167","volume":"165","author":"SJ Eussen","year":"2005","unstructured":"Eussen SJ, de Groot LC, Clarke R et al (2005) Oral cyanocobalamin supplementation in older people with vitamin B12 deficiency: a dose-finding trial. Arch Intern Med 165:1167\u20131172","journal-title":"Arch Intern Med"},{"key":"1125_CR27","doi-asserted-by":"publisher","first-page":"755","DOI":"10.1017\/S000711451200565X","volume":"110","author":"GB Mensink","year":"2013","unstructured":"Mensink GB, Fletcher R, Gurinovic M et al (2013) Mapping low intake of micronutrients across Europe. Br J Nutr 110:755\u2013773","journal-title":"Br J Nutr"},{"key":"1125_CR28","doi-asserted-by":"publisher","first-page":"247","DOI":"10.1111\/j.0954-6820.1968.tb02452.x","volume":"184","author":"H Berlin","year":"1968","unstructured":"Berlin H, Berlin R, Brante G (1968) Oral treatment of pernicious anemia with high doses of vitamin B12 without intrinsic factor. Acta Med Scand 184:247\u2013258","journal-title":"Acta Med Scand"},{"key":"1125_CR29","doi-asserted-by":"publisher","DOI":"10.1007\/s00394-017-1553-5","author":"E Greibe","year":"2017","unstructured":"Greibe E, Mahalle N, Bhide V et al (2017) Increase in circulating holotranscobalamin after oral administration of cyanocobalamin or hydroxocobalamin in healthy adults with low and normal cobalamin status. Eur J Nutr. https:\/\/doi.org\/10.1007\/s00394-017-1553-5","journal-title":"Eur J Nutr"}],"container-title":["Aging Clinical and Experimental Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s40520-019-01125-6\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s40520-019-01125-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s40520-019-01125-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,11,23]],"date-time":"2020-11-23T19:49:04Z","timestamp":1606160944000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s40520-019-01125-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,1,24]]},"references-count":29,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2019,12]]}},"alternative-id":["1125"],"URL":"https:\/\/doi.org\/10.1007\/s40520-019-01125-6","relation":{},"ISSN":["1720-8319"],"issn-type":[{"value":"1720-8319","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,1,24]]},"assertion":[{"value":"19 November 2018","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 January 2019","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 January 2019","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"The authors have no conflicts of interest to declare.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"The study was conducted in accordance with the ethical principles stated in the Declaration of Helsinki. The study protocol was reviewed and approved by the Ethics Committee of the Saarland Region (Approval Number: 244\/14).","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"All procedures were approved by the review board of the Saarland Medical Committee.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Statement of human and animal rights"}},{"value":"All participants provided written informed consent to the study.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Informed consent"}}]}}