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This was a retrospective, non-interventional study conducted at European tertiary referral infertility clinics in women aged 18\u201345\u00a0years, with a body mass index 18\u201335\u00a0kg\/m<jats:sup>2<\/jats:sup>, regular menses, and both ovaries.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>Serum samples were obtained from 230 women classified by oocyte retrieval as poor (33.0%; 0\u20133 oocytes), normal (40.9%; 4\u201315 oocytes), or high (26.1%;\u2009&gt;\u200915 oocytes) responders. E<jats:sub>2<\/jats:sub> and progesterone levels increased during ovarian stimulation, with greatest increases observed in high responders. Elecsys<jats:sup>\u00ae<\/jats:sup> Gen III and Gen II assay results were highly correlated for E<jats:sub>2<\/jats:sub> (Pearson\u2019s <jats:italic>r<\/jats:italic>\u2009=\u20090.99) and progesterone (<jats:italic>r<\/jats:italic>\u2009=\u20090.89); Gen III results were lower than Gen II for both E<jats:sub>2<\/jats:sub> and progesterone. On the day of triggering, Gen III E<jats:sub>2<\/jats:sub> and progesterone levels showed a difference of\u2009\u2212\u200915.0% and\u2009\u2212\u200927.9%, respectively. Progesterone levels (on day of triggering) measured by LC\u2013MS\/MS correlated better with Gen III (0.98) than Gen II (0.90). Mean relative differences for Gen III and Gen II assays versus LC\u2013MS\/MS were 14.6% and 62.8%, respectively.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Conclusion<\/jats:title>\n<jats:p>E<jats:sub>2<\/jats:sub> and progesterone levels determined with Elecsys<jats:sup>\u00ae<\/jats:sup> Gen II and III assays were highly correlated; results were lower for Gen III versus Gen II. Differences observed for progesterone on the day of triggering may be clinically relevant.<\/jats:p>\n<\/jats:sec>","DOI":"10.1007\/s40618-020-01211-x","type":"journal-article","created":{"date-parts":[[2020,3,13]],"date-time":"2020-03-13T08:02:37Z","timestamp":1584086557000},"page":"1239-1248","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["EStradiol and PRogesterone in In vitro ferTilization (ESPRIT): a multicenter study evaluating third- versus second-generation estradiol and progesterone immunoassays"],"prefix":"10.1007","volume":"43","author":[{"given":"N. 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E.A. has nothing to disclose. P.D. has received honoraria for lecturing and\/or research grants from MSD, Ferring International, and Merck. H.T. has received research grants from Merck Serono, MSD, Goodlife, Cook, Roche, Besins, Ferring, Research Fund of Flanders (FWO) and Mirtha (now Allergan), and consultancy fees from Finox, Abbott, ObsEva and Merck. J.S. has nothing to disclose. H.D., G.B., G.M., and W.V., are employees of Roche Diagnostics. E.B. has received fees from Ferring Pharmaceuticals, Merck Serono, MSD, and Roche Diagnostics, and fees and grant support from Finox.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and\/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"Informed consent was obtained from all individual participants included in the study.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Informed consent"}}]}}