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Consequently, the relevance of a sufficiently high VitD serum level becomes self-evident.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>A rapid immunofluorescence assay designed for the point-of-care measurement of serum VitD<jats:sub>3<\/jats:sub> solely was tested. Inter- and intra-assay validation, double testing and result comparison with a standardized laboratory method were performed.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>An overall linear correlation of <jats:italic>r<\/jats:italic>\u2009=\u20090.89 (Pearson, 95% CI 0.88\u20130.92, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01) between the point of care and the conventional reference assay was registered. Accuracy and precision were of special interest at cut-points (10\u00a0ng\/ml [mean deviation 1.7\u00a0ng\/ml, SD 1.98\u00a0ng\/ml, SE 0.16\u00a0ng\/ml], 12\u00a0ng\/ml [MD 0.41, SD 1.89, SE 0.19] and 30\u00a0ng\/ml [MD \u2212\u00a01.11, SD 3.89, SE 0.35]). Only a slight deviation was detected between the two assays when using fresh (<jats:italic>r<\/jats:italic>\u2009=\u20090.91, 95% CI 0.86\u20130.94, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01) and frozen serum samples (<jats:italic>r<\/jats:italic>\u2009=\u20090.86, 0.82\u20130.89, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01). Results remained steady when samples were frozen several times. Inter- and intra-assay validation according to the CLSI protocol as well as multiuser testing showed stable results.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>This novel, innovative, and controlled study indicates that the evaluated rapid point of care VitD assay is reliable, accurate, and suited for clinical practice.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1007\/s40618-021-01575-8","type":"journal-article","created":{"date-parts":[[2021,4,22]],"date-time":"2021-04-22T17:02:41Z","timestamp":1619110961000},"page":"2485-2492","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["A rapid point-of-care assay accurately measures vitamin D"],"prefix":"10.1007","volume":"44","author":[{"given":"K.","family":"Albrecht","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Lotz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Frommer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. 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