{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T23:42:35Z","timestamp":1769038955647,"version":"3.49.0"},"reference-count":34,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2015,11,11]],"date-time":"2015-11-11T00:00:00Z","timestamp":1447200000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"},{"start":{"date-parts":[[2015,11,11]],"date-time":"2015-11-11T00:00:00Z","timestamp":1447200000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Hyperhomocysteinaemia, an independent risk factor for cardiovascular diseases, is common in hemodialysis patients (HD) and particularly in those homozygous for polymorphism of the 5,10-methylenetetrahydrofolate reductase (MTHFR) gene. B vitamins supplementation has been shown to lower plasma total homocysteine (tHcy), but this has been contreversed in several groups. The aim of our study was to explore the response of tHcy in hemodialysis (HD) patients to individual supplementation with folic acid (B9) and\/or vitamin B12, based on carrier status for the (MTHFR) polymorphism.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>132HD were randomized according to C677TMTHFR genotypes into 2 groups (AandB). The group (A) was treated initially with B9 (10mg\/day orally) for 2\u00a0months (t1) and then with B12 vitamin (cyanocobalamin ampoule of 1000\u00a0\u03bcg) for the following 2\u00a0months (t2), then association of B9 and B12 for 2\u00a0months (t3). The group (B) was supplemented initially with vitamin B12 (t1), then with folic acid (t2) and then B9\u00a0+\u00a0B12 for 2\u00a0months (t3). A wash-out period of 2\u00a0months followed the treatment in both groups (t4). We determined tHcy, B9 and B12 concentrations at each time.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>In group A, we noted that the decrease in tHcy becomes significant for CC when patients were supplemented with vit B12 only (p\u00a0=\u00a00.009). While, B9\u00a0+\u00a0vit B12 supplementation did not seem to improve a significant effect compared with B12 alone. For genotypes (CT) and (TT) we noticed a significant decrease in tHcy at t1 (p\u00a0=\u00a00.038; 0.005 respectively) and at (t3; CT p\u00a0=\u00a00.024; TT p\u00a0=\u00a00.017). In group B, for genotypes CC, the decrease in tHcy became significant at t3 (vit B12\u00a0+\u00a0B9; p\u00a0=\u00a00.031). For genotypes (CT) and (TT), at the replacement of vit B12 by B9, tHcy was significantly decreased (p\u00a0=\u00a00.036; 0.012, respectively). The combination of the 2 vitamins (t3) showed no difference compared to folate alone. In the 2 groups (t4), there was an significant increase of tHcy again for 3 genotypes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Supplementation with B vitamins correlated to the MTHFR genotypes has been shown to lower significantly tHcy in HD patients.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s40620-015-0235-8","type":"journal-article","created":{"date-parts":[[2015,11,11]],"date-time":"2015-11-11T07:39:42Z","timestamp":1447227582000},"page":"691-698","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["The C677T MTHFR genotypes influence the efficacy of B9 and B12 vitamins supplementation to lowering plasma total homocysteine in hemodialysis"],"prefix":"10.1093","volume":"29","author":[{"given":"Ons","family":"Achour","sequence":"first","affiliation":[{"name":"Biochemistry Department, Lr 12 Sp11, Sahloul University Hospital, 4054, Sousse, Tunisia 1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sahbi","family":"Elmtaoua","sequence":"additional","affiliation":[{"name":"Nephrology Department, Sahloul University Hospital, Sousse, Tunisia 2"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dorsaf","family":"Zellama","sequence":"additional","affiliation":[{"name":"Nephrology Department, Sahloul University Hospital, Sousse, Tunisia 2"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asma","family":"Omezzine","sequence":"additional","affiliation":[{"name":"Biochemistry Department, Lr 12 Sp11, Sahloul University Hospital, 4054, Sousse, Tunisia 1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amira","family":"Moussa","sequence":"additional","affiliation":[{"name":"Biochemistry Department, Lr 12 Sp11, Sahloul University Hospital, 4054, Sousse, Tunisia 1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jihene","family":"Rejeb","sequence":"additional","affiliation":[{"name":"Biochemistry Department, Lr 12 Sp11, Sahloul University Hospital, 4054, Sousse, Tunisia 1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Imene","family":"Boumaiza","sequence":"additional","affiliation":[{"name":"Biochemistry Department, Lr 12 Sp11, Sahloul University Hospital, 4054, Sousse, Tunisia 1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lobna","family":"Bouacida","sequence":"additional","affiliation":[{"name":"Biochemistry Department, Lr 12 Sp11, Sahloul University Hospital, 4054, Sousse, Tunisia 1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nabila Ben","family":"Rejeb","sequence":"additional","affiliation":[{"name":"Biochemistry Department, Lr 12 Sp11, Sahloul University Hospital, 4054, Sousse, Tunisia 1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdellatif","family":"Achour","sequence":"additional","affiliation":[{"name":"Nephrology Department, Sahloul University Hospital, Sousse, Tunisia 2"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ali","family":"Bouslama","sequence":"additional","affiliation":[{"name":"Biochemistry Department, Lr 12 Sp11, Sahloul University Hospital, 4054, Sousse, Tunisia 1"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2015,11,11]]},"reference":[{"key":"2026012103364942800_CR1"},{"key":"2026012103364942800_CR2"},{"issue":"5 Suppl 3","key":"2026012103364942800_CR3","doi-asserted-by":"publisher","first-page":"S112","DOI":"10.1053\/ajkd.1998.v32.pm9820470 9820470","article-title":"Clinical epidemiology of cardiovascular disease in chronic renal disease","volume":"32","author":"Foley","year":"1998","journal-title":"Am J Kidney Dis"},{"key":"2026012103364942800_CR4","doi-asserted-by":"publisher","first-page":"2520","DOI":"10.1161\/01.CIR.98.23.2520 9843457","article-title":"O\u00a8 hrvik J and Brudin L. 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