{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T04:54:20Z","timestamp":1768625660257,"version":"3.49.0"},"reference-count":13,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2020,8,7]],"date-time":"2020-08-07T00:00:00Z","timestamp":1596758400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2020,8,7]],"date-time":"2020-08-07T00:00:00Z","timestamp":1596758400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2020\/06409-1"],"award-info":[{"award-number":["2020\/06409-1"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2016\/20045-7"],"award-info":[{"award-number":["2016\/20045-7"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Braz J Microbiol"],"published-print":{"date-parts":[[2020,9]]},"DOI":"10.1007\/s42770-020-00347-5","type":"journal-article","created":{"date-parts":[[2020,8,7]],"date-time":"2020-08-07T08:03:28Z","timestamp":1596787408000},"page":"1117-1123","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["Lower cost alternatives for molecular diagnosis of COVID-19: conventional RT-PCR and SYBR Green-based RT-qPCR"],"prefix":"10.1007","volume":"51","author":[{"given":"Erick Gustavo","family":"Dorlass","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cairo Oliveira","family":"Monteiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda Oliveira","family":"Viana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camila Pereira","family":"Soares","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafael Rahal Guaragna","family":"Machado","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luciano Matsumiya","family":"Thomazelli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danielle Bastos","family":"Araujo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabyano Bruno","family":"Leal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erika Donizette","family":"Candido","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruna Larotonda","family":"Telezynski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camila Araujo","family":"Val\u00e9rio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanessa Nascimento","family":"Chalup","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ralyria","family":"Mello","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Flavia Jaqueline","family":"Almeida","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andressa Sim\u00f5es","family":"Aguiar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna Carlotta Mott","family":"Barrientos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolina","family":"Sucupira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Milena","family":"De Paulis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco Aur\u00e9lio Palazzi","family":"S\u00e1fadi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniella Gregorio Bonfim Prado","family":"Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janaina Joice Martins","family":"Sodr\u00e9","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariana Pereira","family":"Soledade","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samantha Faria","family":"Matos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabrina Rodrigues","family":"Ferreira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C\u00e9lia Miranda Nunez","family":"Pinez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolina Palamin","family":"Buonafine","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leticia Nery Ferreira","family":"Pieroni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernanda Mello","family":"Malta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rubia Anita Ferraz","family":"Santana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eloisa Corr\u00eaa","family":"Souza","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo Ambrosio","family":"Fock","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o Renato Rebelo","family":"Pinho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu\u00eds Carlos Souza","family":"Ferreira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Viviane Fongaro","family":"Botosso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edison Luiz","family":"Durigon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0534-0886","authenticated-orcid":false,"given":"Danielle Bruna Leal","family":"Oliveira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,8,7]]},"reference":[{"key":"347_CR1","doi-asserted-by":"publisher","first-page":"727","DOI":"10.1056\/NEJMoa2001017","volume":"382","author":"N Zhu","year":"2020","unstructured":"Zhu N, Zhang D, Wang W, Li X, Yang B, Song J, Zhao X, Huang B, Shi W, Lu R, Niu P, Zhan F, Ma X, Wang D, Xu W, Wu G, Gao GF, Tan W, China Novel Coronavirus Investigating and Research Team (2020) A novel coronavirus from patients with pneumonia in China, 2019. N Engl J Med 382:727\u2013733. https:\/\/doi.org\/10.1056\/NEJMoa2001017","journal-title":"N Engl J Med"},{"key":"347_CR2","unstructured":"Organization WH (2020) Situation Report-83 HIGHLIGHTS"},{"key":"347_CR3","doi-asserted-by":"publisher","first-page":"101613","DOI":"10.1016\/j.tmaid.2020.101613","volume":"35","author":"AJ Rodriguez-Morales","year":"2020","unstructured":"Rodriguez-Morales AJ, Gallego V, Escalera-Antezana JP, M\u00e9ndez CA, Zambrano LI, Franco-Paredes C, Su\u00e1rez JA, Rodriguez-Enciso HD, Balbin-Ramon GJ, Savio-Larriera E, Risquez A, Cimerman S (2020) COVID-19 in Latin America: the implications of the first confirmed case in Brazil. Travel Med Infect Dis. Elsevier USA 35:101613. https:\/\/doi.org\/10.1016\/j.tmaid.2020.101613","journal-title":"Travel Med Infect Dis"},{"key":"347_CR4","unstructured":"World Health Organization (2020) Laboratory testing strategy recommendations for COVID-19"},{"key":"347_CR5","doi-asserted-by":"publisher","first-page":"2000045","DOI":"10.2807\/1560-7917.ES.2020.25.3.2000045","volume":"25","author":"VM Corman","year":"2020","unstructured":"Corman VM, Landt O, Kaiser M, Molenkamp R, Meijer A, Chu DK et al (2020) Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Eurosurveillance 25:2000045. https:\/\/doi.org\/10.2807\/1560-7917.ES.2020.25.3.2000045","journal-title":"Eurosurveillance"},{"key":"347_CR6","doi-asserted-by":"publisher","first-page":"259","DOI":"10.1016\/j.jviromet.2012.07.010","volume":"185","author":"SK Sakthivel","year":"2012","unstructured":"Sakthivel SK, Whitaker B, Lu X, Oliveira DBL, Stockman LJ, Kamili S, Oberste MS, Erdman DD (2012) Comparison of fast-track diagnostics respiratory pathogens multiplex real-time RT-PCR assay with in-house singleplex assays for comprehensive detection of human respiratory viruses. J Virol Methods 185:259\u2013266. https:\/\/doi.org\/10.1016\/j.jviromet.2012.07.010","journal-title":"J Virol Methods"},{"key":"347_CR7","doi-asserted-by":"publisher","unstructured":"Nesteruk I (2020) Long-term predictions for COVID-19 pandemic dynamics in Ukraine, Austria and Italy. medRxiv; 2020.04.08.20058123. https:\/\/doi.org\/10.1101\/2020.04.08.20058123","DOI":"10.1101\/2020.04.08.20058123"},{"key":"347_CR8","doi-asserted-by":"publisher","unstructured":"Ciufolini I, Paolozzi A (2020) Prediction of the time evolution of the Covid-19 pandemic in Italy by a Gauss error function and Monte Carlo simulations. medRxiv; 2020.03.27.20045104. https:\/\/doi.org\/10.1101\/2020.03.27.20045104","DOI":"10.1101\/2020.03.27.20045104"},{"key":"347_CR9","doi-asserted-by":"publisher","first-page":"85","DOI":"10.4103\/2277-9175.127998","volume":"3","author":"M Tajadini","year":"2014","unstructured":"Tajadini M, Panjehpour M, Javanmard S (2014) Comparison of SYBR Green and TaqMan methods in quantitative real-time polymerase chain reaction analysis of four adenosine receptor subtypes. Adv Biomed Res 3:85. https:\/\/doi.org\/10.4103\/2277-9175.127998","journal-title":"Adv Biomed Res"},{"key":"347_CR10","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1101\/gr.6.10.986","volume":"33","author":"DW Kim","year":"2001","unstructured":"Kim DW (2001) Real time quantitative PCR. Exp Mol Med 33:101\u2013109. https:\/\/doi.org\/10.1101\/gr.6.10.986","journal-title":"Exp Mol Med"},{"key":"347_CR11","doi-asserted-by":"publisher","first-page":"478","DOI":"10.4297\/najms.2011.3478","volume":"3","author":"D Paudel","year":"2011","unstructured":"Paudel D, Jarman R, Limkittikul K, Klungthong C, Chamnanchanunt S, Nisalak A, Gibbons R, Chokejindachai W (2011) Comparison of real-time SYBR green dengue assay with real-time taqman RT-PCR dengue assay and the conventional nested PCR for diagnosis of primary and secondary dengue infection. N Am J Med Sci 3:478\u2013485. https:\/\/doi.org\/10.4297\/najms.2011.3478","journal-title":"N Am J Med Sci"},{"key":"347_CR12","doi-asserted-by":"publisher","unstructured":"Zou L, Ruan F, Huang M, Liang L, Huang H, Hong Z, Yu J, Kang M, Song Y, Xia J, Guo Q, Song T, He J, Yen HL, Peiris M, Wu J (2020) SARS-CoV-2 viral load in upper respiratory specimens of infected patients. N Engl J Med. Massachussetts Medical Society:1177\u20131179. https:\/\/doi.org\/10.1056\/NEJMc2001737","DOI":"10.1056\/NEJMc2001737"},{"key":"347_CR13","doi-asserted-by":"publisher","unstructured":"Liu Y, Yan LM, Wan L, Xiang TX, Le A, Liu JM et al (2020) Viral dynamics in mild and severe cases of COVID-19. Lancet Infect Dis. Lancet Publishing Group. https:\/\/doi.org\/10.1016\/S1473-3099(20)30232-2","DOI":"10.1016\/S1473-3099(20)30232-2"}],"container-title":["Brazilian Journal of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s42770-020-00347-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s42770-020-00347-5\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s42770-020-00347-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,8,6]],"date-time":"2021-08-06T23:16:21Z","timestamp":1628291781000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s42770-020-00347-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,7]]},"references-count":13,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2020,9]]}},"alternative-id":["347"],"URL":"https:\/\/doi.org\/10.1007\/s42770-020-00347-5","relation":{},"ISSN":["1517-8382","1678-4405"],"issn-type":[{"value":"1517-8382","type":"print"},{"value":"1678-4405","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8,7]]},"assertion":[{"value":"18 May 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 July 2020","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 August 2020","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"The authors declare that they have no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"Our institutional review board approved this study and all biological samples were collected under the approval of the Ethics Committee of the Institute of Biomedical Science (ICB), USP.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"Not applicable.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Code availability"}},{"value":"This content has been made available to all.","name":"free","label":"Free to read"}]}}