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Heavy metals are notoriously toxic due to their ability to biomagnify in the food chain and interact with cellular components. Nevertheless, some bacteria have physiological mechanisms that enable them to survive in impacted environments. This characteristic makes them important as biotechnological tools for environmental remediation. Thus, we isolated a bacterial consortium in Guanabara Bay (Brazil), a place with a long metal pollution history. To test the growth efficiency of this consortium in Cu\u2013Zn-Pb-Ni\u2013Cd medium, we measured the activity of key enzymes of microbial activity (esterases and dehydrogenase) under acidic (4.0) and neutral pH conditions, as well as the number of living cells, biopolymer production, and changes in microbial composition during metal exposure. Additionally, we calculated the predicted physiology based on microbial taxonomy. During the assay, a slight modification in bacterial composition was observed, with low abundance changes and little production of carbohydrates. <jats:italic>Oceanobacillus<\/jats:italic><jats:italic>chironomi<\/jats:italic>, <jats:italic>Halolactibacillus miurensis<\/jats:italic>, and <jats:italic>Alkaliphilus oremlandii<\/jats:italic> were predominant in pH 7, despite <jats:italic>O. chironomi<\/jats:italic> and <jats:italic>Tissierella creatinophila<\/jats:italic> in pH 4, and <jats:italic>T. creatinophila<\/jats:italic> in Cu\u2013Zn-Pb-Ni\u2013Cd treatment. The metabolism represented by esterases and dehydrogenase enzymes suggested bacterial investment in esterases to capture nutrients and meet the energy demand in an environment with metal stress. Their metabolism potentially shifted to chemoheterotrophy and recycling nitrogenous compounds. Moreover, concomitantly, bacteria produced more lipids and proteins, suggesting extracellular polymeric substance production and growth in a metal-stressed environment. The isolated consortium showed promise for bioremediation of multimetal contamination and could be a valuable tool in future bioremediation programs.<\/jats:p>","DOI":"10.1007\/s42770-023-00993-5","type":"journal-article","created":{"date-parts":[[2023,5,22]],"date-time":"2023-05-22T09:02:49Z","timestamp":1684746169000},"page":"1523-1532","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Biomass and enzymatic activities of marine bacteria in the presence of multiple metals"],"prefix":"10.1007","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3571-5183","authenticated-orcid":false,"given":"J. A. P.","family":"Bitencourt","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. P. 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