{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T23:01:03Z","timestamp":1793660463284,"version":"4.5.1"},"reference-count":37,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2024,3,1]],"date-time":"2024-03-01T00:00:00Z","timestamp":1709251200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2024,3,1]],"date-time":"2024-03-01T00:00:00Z","timestamp":1709251200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/100022965","name":"Minciencias","doi-asserted-by":"crossref","award":["124380864546 - contract CT. FP 80740- 152-2019."],"award-info":[{"award-number":["124380864546 - contract CT. FP 80740- 152-2019."]}],"id":[{"id":"10.13039\/100022965","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Braz J Microbiol"],"published-print":{"date-parts":[[2024,6]]},"DOI":"10.1007\/s42770-023-01226-5","type":"journal-article","created":{"date-parts":[[2024,2,29]],"date-time":"2024-02-29T22:02:12Z","timestamp":1709244132000},"page":"1083-1090","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Quantitative detection of chikungunya, Zika, and dengue viruses by one-step real-time PCR in different cell substrates"],"prefix":"10.1007","volume":"55","author":[{"given":"Andr\u00e9s F.","family":"Cuellar-Quimbaya","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Luisa","family":"Mu\u00f1oz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoelis","family":"Yepez-Perez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingrid DJim\u00e9nez","family":"C","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anny K.","family":"Rodr\u00edguez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nidya Alexandra","family":"Segura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felio","family":"Bello","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1141-1828","authenticated-orcid":false,"given":"M\u00f3nica","family":"Losada-Barrag\u00e1n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,3,1]]},"reference":[{"key":"1226_CR1","first-page":"93","volume":"1","author":"DA \u00c1lvarez-D\u00edaz","year":"2021","unstructured":"\u00c1lvarez-D\u00edaz DA, Valencia-\u00c1lvarez E, Rivera JA, Rengifo AC, Usme-Ciro JA, Pel\u00e1ez-Carvajal D et al (2021) An updated RT-qPCR assay for the simultaneous detection and quantification of chikungunya, dengue and zika viruses. Infect Genet Evol 1:93","journal-title":"Infect Genet Evol"},{"issue":"24","key":"1226_CR2","doi-asserted-by":"publisher","first-page":"2321","DOI":"10.1056\/NEJMoa1602412","volume":"375","author":"P Brasil","year":"2016","unstructured":"Brasil P, Pereira JP, Moreira ME, Ribeiro Nogueira RM, Damasceno L, Wakimoto M et al (2016) Zika Virus Infection in Pregnant Women in Rio de Janeiro. N Engl J Med 375(24):2321\u20132334","journal-title":"N Engl J Med"},{"issue":"16","key":"1226_CR3","doi-asserted-by":"publisher","first-page":"1506","DOI":"10.1056\/NEJMp1602708","volume":"374","author":"N Broutet","year":"2016","unstructured":"Broutet N, Krauer F, Riesen M, Khalakdina A, Almiron M, Aldighieri S et al (2016) Zika Virus as a Cause of Neurologic Disorders. N Engl J Med 374(16):1506\u20131509","journal-title":"N Engl J Med"},{"issue":"83","key":"1226_CR4","doi-asserted-by":"publisher","first-page":"66","DOI":"10.1016\/j.jcv.2016.09.001","volume":"1","author":"K Pabbaraju","year":"2016","unstructured":"Pabbaraju K, Wong S, Gill K, Fonseca K, Tipples GA, Tellier R (2016) Simultaneous detection of Zika, Chikungunya and Dengue viruses by a multiplex real-time RT-PCR assay. J Clin Virol 1(83):66\u201371","journal-title":"J Clin Virol"},{"key":"1226_CR5","doi-asserted-by":"crossref","unstructured":"Whitehorn J, Simmons CP (2011) The pathogenesis of dengue. Vaccine 9(42):7221\u20137228","DOI":"10.1016\/j.vaccine.2011.07.022"},{"issue":"3","key":"1226_CR6","doi-asserted-by":"publisher","first-page":"487","DOI":"10.1128\/CMR.00072-15","volume":"29","author":"D Musso","year":"2016","unstructured":"Musso D, Gubler DJ (2016) Zika virus. Clin Microbiol Rev 29(3):487\u2013524","journal-title":"Clin Microbiol Rev"},{"key":"1226_CR7","doi-asserted-by":"crossref","unstructured":"Gubler DJ (1998) Dengue and dengue hemorrhagic fever. Clin Microbiol Rev 11(3):480\u2013496","DOI":"10.1128\/CMR.11.3.480"},{"key":"1226_CR8","doi-asserted-by":"crossref","unstructured":"Neto SRDS, Oliveira TT, Teixeira IV, De Oliveira SBA, Sampaio VS, Lynn T et al (2022) Machine learning and deep learning techniques to support clinical diagnosis of arboviral diseases: a systematic review. PLoS Negl Trop Dis 16(1)","DOI":"10.1371\/journal.pntd.0010061"},{"key":"1226_CR9","doi-asserted-by":"crossref","unstructured":"Guzman A, Ist\u00fariz RE (2010) Update on the global spread of dengue. Int J Antimicrob Agents 36(1)","DOI":"10.1016\/j.ijantimicag.2010.06.018"},{"issue":"9","key":"1226_CR10","doi-asserted-by":"publisher","first-page":"3459","DOI":"10.1128\/JCM.01288-14","volume":"52","author":"J Nelson","year":"2014","unstructured":"Nelson J, Waggoner JJ, Sahoo MK, Grant PM, Pinsky BA (2014) Encephalitis caused by Chikungunya virus in a traveler from the Kingdom of Tonga. J Clin Microbiol 52(9):3459\u20133461","journal-title":"J Clin Microbiol"},{"key":"1226_CR11","doi-asserted-by":"crossref","unstructured":"Vogels CBF, R\u00fcckert C, Cavany SM, Perkins TA, Ebel GD, Grubaugh ND (2019) Arbovirus coinfection and co-transmission: a neglected public health concern? PLoS Biol 17(1)","DOI":"10.1371\/journal.pbio.3000130"},{"key":"1226_CR12","first-page":"271","volume":"1","author":"DA \u00c1lvarez-D\u00edaz","year":"2019","unstructured":"\u00c1lvarez-D\u00edaz DA, Quintero PA, Pel\u00e1ez-Carvajal D, Ajami NJ, Usme-Ciro JA (2019) Novel pan-serotype control RNA for dengue virus typing through real-time reverse transcription-polymerase chain reaction. J Virol Methods 1:271","journal-title":"J Virol Methods"},{"issue":"9","key":"1226_CR13","first-page":"1","volume":"115","author":"LMC de C\u00f4rtes","year":"2020","unstructured":"de C\u00f4rtes LMC, de Pita-Pereira D, Farani PSG, Pereira BAS, Lopes GD, da Silva FS et al (2020) Insights into the proteomic profile and gene expression of Lutzomyia longipalpis-derived lulo cell line. Mem Inst Oswaldo Cruz 115(9):1\u201323","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"1226_CR14","volume-title":"The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools","author":"JD Thompson","year":"1997","unstructured":"Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools, vol 25. Oxford University Press, Nucleic Acids Research"},{"key":"1226_CR15","first-page":"93","volume":"1","author":"DA \u00c1lvarez-D\u00edaz","year":"2021","unstructured":"\u00c1lvarez-D\u00edaz DA, Valencia-\u00c1lvarez E, Rivera JA, Rengifo AC, Usme-Ciro JA, Pel\u00e1ez-Carvajal D et al (2021) An updated RT-qPCR assay for the simultaneous detection and quantification of chikungunya, dengue and zika viruses. Infect Genet Evol 1:93","journal-title":"Infect Genet Evol"},{"key":"1226_CR16","volume-title":"Minireview: Epidemiological impact of arboviral diseases in Latin American countries, arbovirus-vector interactions and control strategies","author":"NA Segura","year":"2021","unstructured":"Segura NA, Mu\u00f1oz AL, Losada-Barrag\u00e1n M, Torres O, Rodr\u00edguez AK, Rangel H et al (2021) Minireview: Epidemiological impact of arboviral diseases in Latin American countries, arbovirus-vector interactions and control strategies, vol 79. Oxford University Press, Pathogens and Disease"},{"issue":"9","key":"1226_CR17","doi-asserted-by":"publisher","first-page":"6134","DOI":"10.1021\/acsomega.0c04719","volume":"6","author":"C Buendia-Atencio","year":"2021","unstructured":"Buendia-Atencio C, Pieffet GP, Montoya-Vargas S, Mart\u00ednez Bernal JA, Rangel HR, Mu\u00f1oz AL et al (2021) Inverse Molecular Docking Study of NS3-Helicase and NS5-RNA Polymerase of Zika Virus as Possible Therapeutic Targets of Ligands Derived from Marcetia taxifolia and Its Implications to Dengue Virus. ACS Omega 6(9):6134\u20136143","journal-title":"ACS Omega"},{"key":"1226_CR18","doi-asserted-by":"crossref","unstructured":"Hub\u00e1lek Z, Rudolf I, Nowotny N (2014) Arboviruses pathogenic for domestic and wild animals. Adv Virus Res 89(1):201\u2013275","DOI":"10.1016\/B978-0-12-800172-1.00005-7"},{"key":"1226_CR19","doi-asserted-by":"crossref","unstructured":"Yap G, Pok KY, Lai YL, Hapuarachchi HC, Chow A, Leo YS et al (2010) Evaluation of chikungunya diagnostic assays: differences in sensitivity of serology assays in two independent outbreaks. PLoS Negl Trop Dis 4(7)","DOI":"10.1371\/journal.pntd.0000753"},{"key":"1226_CR20","doi-asserted-by":"crossref","unstructured":"Shan C, Ortiz DA, Yang Y, Wong SJ, Kramer LD, Shi PY et al (2017) Evaluation of a novel reporter virus neutralization test for serological diagnosis of Zika and Dengue virus infection. J Clin Microbiol 55(10):3028\u20133036","DOI":"10.1128\/JCM.00975-17"},{"key":"1226_CR21","doi-asserted-by":"crossref","unstructured":"Faye O, Faye O, Diallo D, Diallo M, Weidmann M, Sall AA (2013) Quantitative real-time PCR detection of Zika virus and evaluation with field-caught Mosquitoes. Virol J 10(311)","DOI":"10.1186\/1743-422X-10-311"},{"key":"1226_CR22","unstructured":"Haninah Ali U, Thayan R, Angamuthu C, Han Lim L, Devi Sekaran S (2010) Development and evaluation of a one-step SYBR-Green I-based real-time RT-PCR assay for the detection and quantification of Chikungunya virus in human, monkey and mosquito samples. Trop Biomed 27(1)"},{"key":"1226_CR23","doi-asserted-by":"crossref","unstructured":"Gomes-Ruiz AC, Nascimento RT, De Paula SO, Lopes Da Fonseca BA (2006) SYBR green and TaqMan real-time PCR assays are equivalent for the diagnosis of dengue virus type 3 infections. J Med Virol 78(6):760\u2013763","DOI":"10.1002\/jmv.20620"},{"key":"1226_CR24","doi-asserted-by":"crossref","unstructured":"San Ho P, Mah M, Ng L, Jang J, Chu H (2010) Establishment of one-step SYBR green-based real time-PCR assay for rapid detection and quantification of chikungunya virus infection. Virol J 7(13)","DOI":"10.1186\/1743-422X-7-13"},{"key":"1226_CR25","doi-asserted-by":"crossref","unstructured":"Salles TS, S\u00e1-Guimar\u00e3es TE, Souza DFS, L\u00f3pez SBG, Alvarenga ESL, Franco TA et al (2017) Quantitative dengue serotyping: the development of a higher performance method using SYBR green assay. Arch Clin Microbiol 08(04)","DOI":"10.4172\/1989-8436.100055"},{"issue":"6","key":"1226_CR26","doi-asserted-by":"publisher","first-page":"722","DOI":"10.1016\/j.jmoldx.2015.06.008","volume":"17","author":"H Chen","year":"2015","unstructured":"Chen H, Parimelalagan M, Lai YL, Lee KS, Koay ESC, Hapuarachchi HC et al (2015) Development and Evaluation of a SYBR Green-Based Real-Time Multiplex RT-PCR Assay for Simultaneous Detection and Serotyping of Dengue and Chikungunya Viruses. Journal of Molecular Diagnostics 17(6):722\u2013728","journal-title":"Journal of Molecular Diagnostics"},{"issue":"4","key":"1226_CR27","doi-asserted-by":"publisher","first-page":"1511","DOI":"10.1128\/JCM.42.4.1511-1518.2004","volume":"42","author":"JF Papin","year":"2004","unstructured":"Papin JF, Vahrson W, Dittmer DP (2004) SYBR Green-Based Real-Time Quantitative PCR Assay for Detection of West Nile Virus Circumvents False-Negative Results Due to Strain Variability. J Clin Microbiol 42(4):1511\u20131518","journal-title":"J Clin Microbiol"},{"issue":"83","key":"1226_CR28","doi-asserted-by":"publisher","first-page":"66","DOI":"10.1016\/j.jcv.2016.09.001","volume":"1","author":"K Pabbaraju","year":"2016","unstructured":"Pabbaraju K, Wong S, Gill K, Fonseca K, Tipples GA, Tellier R (2016) Simultaneous detection of Zika, Chikungunya and Dengue viruses by a multiplex real-time RT-PCR assay. J Clin Virol 1(83):66\u201371","journal-title":"J Clin Virol"},{"key":"1226_CR29","doi-asserted-by":"crossref","unstructured":"Dash PK, Boutonnier A, Prina E, Sharma S, Reiter P (2012) Development of a SYBR green I based RT-PCR assay for yellow fever virus: application in assessment of YFV infection in Aedes aegypti. Virol J 9(1)","DOI":"10.1186\/1743-422X-9-27"},{"issue":"82","key":"1226_CR30","doi-asserted-by":"publisher","first-page":"108","DOI":"10.1016\/j.jcv.2016.07.017","volume":"1","author":"MJ Cabral-Castro","year":"2016","unstructured":"Cabral-Castro MJ, Cavalcanti MG, Peralta RHS, Peralta JM (2016) Molecular and serological techniques to detect co-circulation of DENV, ZIKV and CHIKV in suspected dengue-like syndrome patients. J Clin Virol 1(82):108\u2013111","journal-title":"J Clin Virol"},{"key":"1226_CR31","doi-asserted-by":"crossref","unstructured":"Souza NCS e., F\u00e9lix AC, de Paula AV, Levi JE, Pannuti CS, Romano CM (2019) Evaluation of serological cross-reactivity between yellow fever and other flaviviruses. International Journal of Infectious Diseases. 81:4\u20135.","DOI":"10.1016\/j.ijid.2019.01.023"},{"key":"1226_CR32","first-page":"307","volume":"1","author":"TR Barnard","year":"2022","unstructured":"Barnard TR, Wang AB, Sagan SM (2022) A highly sensitive strand-specific multiplex RT-qPCR assay for quantitation of Zika virus replication. J Virol Methods 1:307","journal-title":"J Virol Methods"},{"key":"1226_CR33","doi-asserted-by":"crossref","unstructured":"Bhatnagar P, Sreekanth GP, Murali-Krishna K, Chandele A, Sitaraman (2021) Dengue virus non-structural protein 5 as a versatile, multi-functional effector in host\u2013pathogen interactions. Front Cell Infect Microbiol 8(11)","DOI":"10.3389\/fcimb.2021.574067"},{"key":"1226_CR34","doi-asserted-by":"crossref","unstructured":"Ahola T, Merits A (2016) Functions of chikungunya virus nonstructural proteins. Chikungunya Virus 3:75\u201398","DOI":"10.1007\/978-3-319-42958-8_6"},{"issue":"4","key":"1226_CR35","doi-asserted-by":"publisher","first-page":"505","DOI":"10.4269\/ajtmh.2008.79.505","volume":"79","author":"SJ Wu","year":"2008","unstructured":"Wu SJ, Pal S, Ekanayake S, Greenwald D, Lara S, Raviprakash K et al (2008) A dry-format field-deployable quantitative reverse transcriptase-polymerase chain reaction assay for diagnosis of dengue infections. Am J Trop Med Hyg 79(4):505\u2013510","journal-title":"Am J Trop Med Hyg"},{"key":"1226_CR36","doi-asserted-by":"crossref","unstructured":"Gurukumar K, Priyadarshini D, Patil J, Bhagat A, Singh A, Shah P et al (2009) Development of real time PCR for detection and quantitation of Dengue Viruses. Virol J 23(6)","DOI":"10.1186\/1743-422X-6-10"},{"key":"1226_CR37","doi-asserted-by":"crossref","unstructured":"Cuevas-Reyes E, Carrillo-Morales M, Trevi\u00f1o-Quintanilla LG, Aparicio-Fabre R, Hern\u00e1ndez-Romano J (2016) Oligonucleotides evaluation for measuring gene expression during tomato bacterial wilt. Rev Fitotec Mex 39(2):141\u2013150","DOI":"10.35196\/rfm.2016.2.141-150"}],"container-title":["Brazilian Journal of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s42770-023-01226-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s42770-023-01226-5\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s42770-023-01226-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,5]],"date-time":"2024-06-05T22:10:52Z","timestamp":1717625452000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s42770-023-01226-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,1]]},"references-count":37,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024,6]]}},"alternative-id":["1226"],"URL":"https:\/\/doi.org\/10.1007\/s42770-023-01226-5","relation":{},"ISSN":["1517-8382","1678-4405"],"issn-type":[{"value":"1517-8382","type":"print"},{"value":"1678-4405","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3,1]]},"assertion":[{"value":"4 September 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 December 2023","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 March 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}