{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T19:30:01Z","timestamp":1757619001209,"version":"3.44.0"},"reference-count":38,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2025,7,15]],"date-time":"2025-07-15T00:00:00Z","timestamp":1752537600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,7,15]],"date-time":"2025-07-15T00:00:00Z","timestamp":1752537600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"name":"Institutional Program to Support Research Groups Policies","award":["EDITAL N 118\/2024\u2013 PRPPG\/UNILA"],"award-info":[{"award-number":["EDITAL N 118\/2024\u2013 PRPPG\/UNILA"]}]},{"name":"Assistance to Research Activities","award":["PRPPG N 121\/2023"],"award-info":[{"award-number":["PRPPG N 121\/2023"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Braz J Microbiol"],"published-print":{"date-parts":[[2025,9]]},"DOI":"10.1007\/s42770-025-01737-3","type":"journal-article","created":{"date-parts":[[2025,7,15]],"date-time":"2025-07-15T14:48:09Z","timestamp":1752590889000},"page":"1977-1984","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Microbial profiling of swine-waste digestates: implications for biofertilizer application"],"prefix":"10.1007","volume":"56","author":[{"given":"Jo\u00e3o Victor Pessoa","family":"Maia","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Franciele Natividade","family":"Luiz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marahia Isabel Guevara","family":"Robayo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deborah Catharine","family":"de Assis Leite","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafaela Faust","family":"Meyer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8614-1896","authenticated-orcid":false,"given":"Michel Rodrigo Zambrano","family":"Passarini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,7,15]]},"reference":[{"issue":"2","key":"1737_CR1","doi-asserted-by":"publisher","first-page":"452","DOI":"10.1016\/j.biotechadv.2018.01.011","volume":"36","author":"I Angelidaki","year":"2018","unstructured":"Angelidaki I, Treu L, Tsapekos P, Luo G, Campanaro S, Wenzel H, Kougias PG (2018) Biogas upgrading and utilization: current status and perspectives. Biotechnol Adv 36(2):452\u2013466","journal-title":"Biotechnol Adv"},{"issue":"75","key":"1737_CR2","doi-asserted-by":"publisher","first-page":"29011","DOI":"10.1016\/j.ijhydene.2023.03.240","volume":"48","author":"A Nemmour","year":"2023","unstructured":"Nemmour A, Inayat A, Janajreh I, Ghenai C (2023) Green hydrogen-based E-fuels (E-methane, E-methanol, E-ammonia) to support clean energy transition: A literature review. Int J Hyd Energ 48(75):29011\u201329033","journal-title":"Int J Hyd Energ"},{"issue":"7","key":"1737_CR3","doi-asserted-by":"publisher","first-page":"184","DOI":"10.1007\/s11274-023-03573-9","volume":"39","author":"FN Luiz","year":"2023","unstructured":"Luiz FN, Passarini MRZ, Magrini FE, Gaio J, Somer JG, Meyer RF, Paesi S (2023) Metataxonomic characterization of the microbial community involved in the production of biogas with microcrystalline cellulose in pilot and laboratory scale. World J Microbiol Biotechnol 39(7):184","journal-title":"World J Microbiol Biotechnol"},{"key":"1737_CR4","doi-asserted-by":"publisher","first-page":"102508","DOI":"10.1016\/j.rineng.2024.102508","volume":"23","author":"F Essalhi","year":"2024","unstructured":"Essalhi F, Naourani A, Essadek A, Bengueddour R (2024) Agronomic and energy value of digestate from anaerobic digestion of trout byproducts: contribution to the autonomy of freshwater farms in Morocco. Results Eng 23:102508","journal-title":"Results Eng"},{"key":"1737_CR5","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1016\/j.biombioe.2012.02.018","volume":"40","author":"JA Alburquerque","year":"2012","unstructured":"Alburquerque JA, de la Fuente C, Ferrer-Costa A, Carrasco L, Cegarra J, Abad M, Bernal MP (2012) Assessment of the fertiliser potential of digestates from farm and a agroindustrial residues. Biomass Bioenergy 40:181\u2013189","journal-title":"Biomass Bioenergy"},{"key":"1737_CR6","unstructured":"Taiz L, Zeiger E, M\u00f8ller IM, Murphy A (2017) Fisiologia e desenvolvimento vegetal. Artmed Editora"},{"issue":"3","key":"1737_CR7","first-page":"55","volume":"2","author":"\u00d3GV Torres","year":"2016","unstructured":"Torres \u00d3GV (2016) Importancia de Los Fosfatos y Fosfitos En La nutrici\u00f3n de cultivos. Acta Agr\u00edcola Y Pecuaria 2(3):55\u201361","journal-title":"Acta Agr\u00edcola Y Pecuaria"},{"key":"1737_CR8","doi-asserted-by":"crossref","unstructured":"Al Seadi T, Drosg B, Fuchs W, Rutz D, Janssen R (2013) Biogas digestate quality and utilization. In The biogas handbook, Woodhead publishing, pp. 267\u2013301","DOI":"10.1533\/9780857097415.2.267"},{"key":"1737_CR9","doi-asserted-by":"publisher","first-page":"333","DOI":"10.3389\/fbioe.2020.00333","volume":"8","author":"DC T\u00e1pparo","year":"2020","unstructured":"T\u00e1pparo DC, Rogovski P, Cadamuro RD, Marques Souza DS, Bonatto C, Frumi Camargo A, Fongaro G (2020a) Nutritional, energy and sanitary aspects of swine manure and carcass co-digestion. Front Bioeng Biotechnol 8:333","journal-title":"Front Bioeng Biotechnol"},{"key":"1737_CR10","doi-asserted-by":"publisher","first-page":"105105","DOI":"10.1016\/j.apsoil.2023.105105","volume":"193","author":"F Vautrin","year":"2024","unstructured":"Vautrin F, Piveteau P, Cannavacciuolo M, Barr\u00e9 P, Chauvin C, Villenave C, Sadet-Bourgeteau S (2024) The short-term response of soil microbial communities to digestate application depends on the characteristics of the digestate and soil type. Appl Soil Ecol 193:105105","journal-title":"Appl Soil Ecol"},{"key":"1737_CR11","doi-asserted-by":"publisher","first-page":"103403","DOI":"10.1016\/j.apsoil.2019.103403","volume":"147","author":"JJ Coelho","year":"2020","unstructured":"Coelho JJ, Hennessy A, Casey I, Bragan\u00e7a CRS, Woodcock T, Kennedy N (2020) Biofertilisation with anaerobic digestates: A field study of effects on soil microbial abundance and diversity. Appl Soil Ecol 147:103403","journal-title":"Appl Soil Ecol"},{"key":"1737_CR12","doi-asserted-by":"publisher","first-page":"160","DOI":"10.3390\/fermentation9020160","volume":"9","author":"A Roopnarain","year":"2023","unstructured":"Roopnarain A, Akindolire MA, Rama H, Ndaba B (2023) Casting light on the micro-organisms in digestate: diversity and untapped potential. Fermentation 9:160","journal-title":"Fermentation"},{"key":"1737_CR13","doi-asserted-by":"publisher","first-page":"1031","DOI":"10.1007\/s10295-020-02326-z","volume":"47","author":"JD Fernandez-Bayo","year":"2020","unstructured":"Fernandez-Bayo JD, Simmons CW, VanderGheynst JS (2020) Characterization of digestate microbial community structure following thermophilic anaerobic digestion with varying levels of green and food wastes. J Industrial Microbiol Biotechnology: Official J Soc Industrial Microbiol Biotechnol 47:1031\u20131044","journal-title":"J Industrial Microbiol Biotechnology: Official J Soc Industrial Microbiol Biotechnol"},{"key":"1737_CR14","unstructured":"APHA (2017) Standard methods for the examination of water and wastewater (23rd ed.). Washington DC: American Public Health Association"},{"issue":"4","key":"1737_CR15","doi-asserted-by":"publisher","first-page":"1125","DOI":"10.1093\/bib\/bbx120","volume":"20","author":"FP Breitwieser","year":"2019","unstructured":"Breitwieser FP, Lu J, Salzberg SL (2019) A review of methods and databases for metagenomic classification and assembly. Brief Bioinform 20(4):1125\u20131136","journal-title":"Brief Bioinform"},{"key":"1737_CR16","unstructured":"Joshi NA, Sickle FJN (2011) A sliding-window, adaptive, quality-based trimming tool for FastQ files (Version 1.33) [Software]"},{"key":"1737_CR17","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/gb-2014-15-3-r46","volume":"15","author":"DE Wood","year":"2014","unstructured":"Wood DE, Salzberg SL (2014) Kraken: ultrafast metagenomic sequence classification using exact alignments. Genome Biol 15:1\u201312","journal-title":"Genome Biol"},{"key":"1737_CR18","volume-title":"RA Language and environment for statistical computing","author":"RC Team","year":"2020","unstructured":"Team RC (2020) RA Language and environment for statistical computing. R Foundation for Statistical, Computing"},{"key":"1737_CR19","doi-asserted-by":"publisher","first-page":"1303","DOI":"10.1093\/bioinformatics\/btz715","volume":"36","author":"FP Breitwieser","year":"2020","unstructured":"Breitwieser FP, Salzberg SL (2020) Pavian: interactive analysis of metagenomics data for Microbiome studies and pathogen identification. Bioinform 36:1303\u20131304","journal-title":"Bioinform"},{"issue":"1","key":"1737_CR20","doi-asserted-by":"publisher","first-page":"1","DOI":"10.61762\/ijbrvol2iss1art13847","volume":"2","author":"AR Sahito","year":"2013","unstructured":"Sahito AR, Mahar RB, Siddiqui Z, Brohi KM (2013) Estimating calorific values of lignocellulosic biomass from volatile and fixed solids. Int J Biomass Renew 2(1):1\u20136","journal-title":"Int J Biomass Renew"},{"key":"1737_CR21","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1016\/j.wasman.2018.02.022","volume":"75","author":"ME Kirby","year":"2018","unstructured":"Kirby ME, Theodorou MK, Brizuela CM, Huntington JA, Powles J, Wilkinson RG (2018) The anaerobic digestion of pig carcase with or without sugar beet pulp, as a novel on-farm disposal method. Waste Manag 75:251\u2013260. https:\/\/doi.org\/10.1016\/j.wasman.2018.02.022","journal-title":"Waste Manag"},{"key":"1737_CR22","doi-asserted-by":"publisher","unstructured":"da Silva JFF, T\u00e1pparo DC, Antes FG, Steinmetz RLR, Kunz A (2024) Anaerobic Co-Digestion of swine manure and hydrolyzed swine carcasses at two different biodigesters, 29 February 2024, PREPRINT (Version 1) available. https:\/\/doi.org\/10.21203\/rs.3.rs-3973480\/v1]. at Research Square [","DOI":"10.21203\/rs.3.rs-3973480\/v1]"},{"issue":"1","key":"1737_CR23","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1007\/s10123-024-00530-w","volume":"28","author":"A Kumar","year":"2025","unstructured":"Kumar A, Sharma S, Dindhoria K, Thakur A, Kumar R (2025) Insight into physico-chemical properties and microbial community structure of biogas slurry from household biogas plants of sub-Himalaya for its implications in improved biogas production. Int Microbiol 28(1):187\u2013200","journal-title":"Int Microbiol"},{"key":"1737_CR24","doi-asserted-by":"crossref","unstructured":"Kim BR, Shin J, Guevarra RB, Lee JH, Kim DW, Seol KH, Isaacson. RE (2017) Deciphering diversity indices for a better Understanding of microbial communities. J Microbiol Biotechnol 27(12):2089\u20132093","DOI":"10.4014\/jmb.1709.09027"},{"issue":"2","key":"1737_CR25","doi-asserted-by":"publisher","first-page":"160","DOI":"10.3390\/fermentation9020160","volume":"9","author":"A Roopnarain","year":"2023","unstructured":"Roopnarain A, Akindolire MA, Rama H, Ndaba B (2023) Casting light on the micro-organisms in digestate: diversity and untapped potential. Fermentation 9(2):160","journal-title":"Fermentation"},{"key":"1737_CR26","doi-asserted-by":"publisher","first-page":"152","DOI":"10.1016\/j.biortech.2015.08.004","volume":"197","author":"RM Duda","year":"2015","unstructured":"Duda RM, da Silva Vantini J, Martins LS, de Mello Varani A, Lemos MVF, Ferro MIT, de Oliveira RA (2015) A balanced microbiota efficiently produces methane in a novel high-rate horizontal anaerobic reactor for the treatment of swine wastewater. Biores Technol 197:152\u2013160","journal-title":"Biores Technol"},{"issue":"9","key":"1737_CR27","doi-asserted-by":"publisher","first-page":"1453","DOI":"10.5713\/ab.23.0140","volume":"36","author":"M Miguel","year":"2023","unstructured":"Miguel M, Kim SH, Lee SS, Cho YI (2023) Composition and functional diversity of bacterial communities during swine carcass decomposition. Anim Biosc 36(9):1453","journal-title":"Anim Biosc"},{"issue":"6","key":"1737_CR28","doi-asserted-by":"publisher","first-page":"771","DOI":"10.1007\/s10295-016-1760-8","volume":"43","author":"S Chen","year":"2016","unstructured":"Chen S, Cheng H, Wyckoff KN, He Q (2016) Linkages of Firmicutes and Bacteroidetes populations to methanogenic process performance. J Ind Microbiol Biotechnol 43(6):771\u2013781","journal-title":"J Ind Microbiol Biotechnol"},{"issue":"10","key":"1737_CR29","doi-asserted-by":"publisher","first-page":"4699","DOI":"10.1007\/s00253-016-7436-5","volume":"100","author":"T Kern","year":"2016","unstructured":"Kern T, Theiss J, R\u00f6ske K, Rother M (2016) Assessment of hydrogen metabolism in commercial anaerobic digesters. Appl Microbiol Biotechnol 100(10):4699\u20134710","journal-title":"Appl Microbiol Biotechnol"},{"key":"1737_CR30","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s40168-019-0780-9","volume":"8","author":"X Zhu","year":"2020","unstructured":"Zhu X, Campanaro S, Treu L, Seshadri R, Ivanova N, Kougias PG, Angelidaki I (2020) Metabolic dependencies govern microbial syntrophies during methanogenesis in an anaerobic digestion ecosystem. Microbiome 8:1\u201314","journal-title":"Microbiome"},{"key":"1737_CR31","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13068-020-01776-y","volume":"13","author":"L Braga Nan","year":"2020","unstructured":"Braga Nan L, Trably E, Santa-Catalina G, Bernet N, Delgen\u00e8s JP, Escudi\u00e9 R (2020) Biomethanation processes: new insights on the effect of a high H 2 partial pressure on microbial communities. Biotechnol Biofuels 13:1\u201317","journal-title":"Biotechnol Biofuels"},{"key":"1737_CR32","doi-asserted-by":"crossref","unstructured":"Aasfar A, Meftah Kadmiri I, Azaroual SE, Lemriss S, Mernissi NE, Bargaz A, Hilali. A (2024) Agronomic advantage of bacterial biological nitrogen fixation on wheat plant growth under contrasting nitrogen and phosphorus regimes. Front Plant Sci 15:1388775","DOI":"10.3389\/fpls.2024.1388775"},{"issue":"1","key":"1737_CR33","doi-asserted-by":"publisher","first-page":"1039","DOI":"10.1038\/s42003-023-05391-z","volume":"6","author":"Y Xia","year":"2023","unstructured":"Xia Y, Yuan Y, Li C, Sun Z (2023) Phosphorus-solubilizing bacteria improve the growth of Nicotiana benthamiana on lunar regolith simulant by dissociating insoluble inorganic phosphorus. Commun Biol 6(1):1039","journal-title":"Commun Biol"},{"issue":"1","key":"1737_CR34","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1007\/s10482-024-01961-1","volume":"117","author":"C Cort\u00e9s-Albayay","year":"2024","unstructured":"Cort\u00e9s-Albayay C, Delgado-Torres M, Larama G, Paredes-Negron C, de la Luz Mora M, Dur\u00e1n P, Barra PJ (2024) Comparative genomics of plant growth promoting phosphobacteria isolated from acidic soils. Antonie Van Leeuwenhoek 117(1):76","journal-title":"Antonie Van Leeuwenhoek"},{"issue":"3","key":"1737_CR35","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1007\/s00425-021-03695-0","volume":"254","author":"R Soni","year":"2021","unstructured":"Soni R, Keharia H (2021) Phytostimulation and biocontrol potential of Gram-positive endospore-forming Bacilli. Planta 254(3):49","journal-title":"Planta"},{"key":"1737_CR36","doi-asserted-by":"publisher","first-page":"555","DOI":"10.1016\/j.scitotenv.2019.06.283","volume":"688","author":"XC Yang","year":"2019","unstructured":"Yang XC, Han ZZ, Ruan XY, Chai J, Jiang SW, Zheng R (2019) Composting swine carcasses with nitrogen transformation microbial strains: succession of microbial community and nitrogen functional genes. Sci Total Environ 688:555\u2013566","journal-title":"Sci Total Environ"},{"issue":"6","key":"1737_CR37","doi-asserted-by":"publisher","first-page":"340","DOI":"10.1007\/s00203-022-02819-8","volume":"204","author":"JR Ottoni","year":"2022","unstructured":"Ottoni JR, Bernal SPF, Marteres TJ, Luiz FN, Dos Santos VP, Mari \u00c2G, Passarini, MR Z (2022) Cultured and uncultured microbial community associated with biogas production in anaerobic digestion processes. Arch Microbiol 204(6):340","journal-title":"Arch Microbiol"},{"issue":"6","key":"1737_CR38","doi-asserted-by":"publisher","first-page":"682","DOI":"10.1080\/09593330.2018.1508256","volume":"41","author":"DC T\u00e1pparo","year":"2020","unstructured":"T\u00e1pparo DC, Viancelli A, do Amaral AC, Fongaro G, Steinmetz RLR, Magri ME, Kunz A (2020b) Sanitary effectiveness and biogas yield by anaerobic co-digestion of swine carcasses and manure. Environ Technol 41(6):682\u2013690","journal-title":"Environ Technol"}],"container-title":["Brazilian Journal of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s42770-025-01737-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s42770-025-01737-3\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s42770-025-01737-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,7]],"date-time":"2025-09-07T11:16:46Z","timestamp":1757243806000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s42770-025-01737-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,15]]},"references-count":38,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,9]]}},"alternative-id":["1737"],"URL":"https:\/\/doi.org\/10.1007\/s42770-025-01737-3","relation":{},"ISSN":["1517-8382","1678-4405"],"issn-type":[{"type":"print","value":"1517-8382"},{"type":"electronic","value":"1678-4405"}],"subject":[],"published":{"date-parts":[[2025,7,15]]},"assertion":[{"value":"7 May 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 July 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 July 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"the authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"This study was financed by the Institutional Program to Support Research Groups NOTICE NO 118\/2024 and Assistance to Research Activities (PRPPG N 121\/2023)\u2013 PRPPG\/UNILA.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Financial support"}}]}}