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Here, we analyzed 34 <jats:italic>S. albidoflavus<\/jats:italic> genomes, including the antifungal strain SM254 and 33 publicly available references, to investigate its metabolic potential and functional distinctiveness. Using pangenome reconstruction, phylogenomics, average nucleotide identity, and KEGG pathway profiling, we found that <jats:italic>S. albidoflavus<\/jats:italic> SM254 shares high nucleotide identity (&gt;\u200999%) with five closely related strains but displays a unique combination of complete ethanol fermentation capacity and asparagine biosynthesis deficiency. These traits were exclusive to SM254 and may reflect adaptation to the oxygen-limited, nutrient-variable sediment environment. Functional annotation further revealed high completeness in central energy, redox, and stress-response pathways. Although direct antifungal mechanisms remain to be experimentally validated, <jats:italic>S. albidoflavus<\/jats:italic> SM254\u2019s unique metabolic profile and ecological specialization suggest potential relevance in biocontrol contexts.<\/jats:p>","DOI":"10.1007\/s42770-025-01740-8","type":"journal-article","created":{"date-parts":[[2025,7,22]],"date-time":"2025-07-22T11:12:28Z","timestamp":1753182748000},"page":"2121-2131","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Genomic insights into Streptomyces albidoflavus SM254: tracing the putative signs of anti-Pseudogymnoascus destructans properties"],"prefix":"10.1007","volume":"56","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7947-1654","authenticated-orcid":false,"given":"Ilia V.","family":"Popov","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7265-4562","authenticated-orcid":false,"given":"Michael L.","family":"Chikindas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9223-8731","authenticated-orcid":false,"given":"Igor V.","family":"Popov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,7,22]]},"reference":[{"key":"1740_CR1","doi-asserted-by":"publisher","first-page":"e00148","DOI":"10.1128\/mSphere.00148-16","volume":"1","author":"JM Lorch","year":"2016","unstructured":"Lorch JM, Palmer JM, Lindner DL et al (2016) First detection of Bat White-Nose syndrome in Western North America. mSphere 1:e00148\u2013e00116. https:\/\/doi.org\/10.1128\/mSphere.00148-16","journal-title":"mSphere"},{"key":"1740_CR2","doi-asserted-by":"publisher","first-page":"376","DOI":"10.1038\/nature10590","volume":"480","author":"JM Lorch","year":"2011","unstructured":"Lorch JM, Meteyer CU, Behr MJ et al (2011) Experimental infection of bats with geomyces destructans causes white-nose syndrome. 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