{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T11:15:55Z","timestamp":1764242155367,"version":"3.46.0"},"reference-count":65,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2025,8,6]],"date-time":"2025-08-06T00:00:00Z","timestamp":1754438400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,8,6]],"date-time":"2025-08-06T00:00:00Z","timestamp":1754438400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"name":"Indian Veterinary Research Institute, Izatnagar","award":["Indian Veterinary Research Institute, Izatnagar"],"award-info":[{"award-number":["Indian Veterinary Research Institute, Izatnagar"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Braz J Microbiol"],"published-print":{"date-parts":[[2025,12]]},"DOI":"10.1007\/s42770-025-01747-1","type":"journal-article","created":{"date-parts":[[2025,8,6]],"date-time":"2025-08-06T13:22:17Z","timestamp":1754486537000},"page":"2855-2864","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Isolation of Brucella suis from cattle in India: whole genome analysis unravels potential for zoonotic transmission"],"prefix":"10.1007","volume":"56","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5396-6413","authenticated-orcid":false,"given":"M. Suman","family":"Kumar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1048-6377","authenticated-orcid":false,"given":"S. S. R.","family":"Vakamalla","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haris","family":"Ayoub","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Lalsangzuala","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. R.","family":"Chandni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4200-896X","authenticated-orcid":false,"given":"Himani","family":"Dhanze","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valil","family":"Kunjukunju Vinod","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9828-048X","authenticated-orcid":false,"given":"S.","family":"Anbazhagan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5602-7710","authenticated-orcid":false,"given":"Prasad","family":"Thomas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,8,6]]},"reference":[{"key":"1747_CR1","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1186\/s12889-017-5016-y","volume":"18","author":"KA Franc","year":"2018","unstructured":"Franc KA, Krecek RC, H\u00e4sler BN, Arenas-Gamboa AM (2018) Brucellosis remains a neglected disease in the developing world: a call for interdisciplinary action. BMC Public Health 18:125. https:\/\/doi.org\/10.1186\/s12889-017-5016-y","journal-title":"BMC Public Health"},{"key":"1747_CR2","doi-asserted-by":"publisher","first-page":"1789","DOI":"10.3201\/eid2909.230052","volume":"29","author":"CG Laine","year":"2023","unstructured":"Laine CG, Johnson VE, Scott HM, Arenas-Gamboa AM (2023) Global estimate of human brucellosis incidence. Emerg Infect Dis 29:1789","journal-title":"Emerg Infect Dis"},{"key":"1747_CR3","doi-asserted-by":"publisher","first-page":"16586","DOI":"10.1038\/s41598-023-43570-4","volume":"13","author":"H Brangsch","year":"2023","unstructured":"Brangsch H, Horstkotte MA, Melzer F (2023) Genotypic peculiarities of a human brucellosis case caused by Brucella suis biovar 5. Sci Rep 13:16586","journal-title":"Sci Rep"},{"key":"1747_CR4","doi-asserted-by":"publisher","first-page":"443","DOI":"10.1073\/pnas.221575398","volume":"99","author":"VG DelVecchio","year":"2002","unstructured":"DelVecchio VG, Kapatral V, Redkar RJ, Patra G, Mujer C, Los T, Ivanova N, Anderson I, Bhattacharyya A, Lykidis A, Reznik G, Jablonski L, Larsen N, D\u2019Souza M, Bernal A, Mazur M, Goltsman E, Selkov E, Elzer PH, Hagius S, O\u2019Callaghan D, Letesson J-J, Haselkorn R, Kyrpides N, Overbeek R (2002) The genome sequence of the facultative intracellular pathogen Brucella melitensis. Proc Natl Acad Sci USA 99:443\u2013448. https:\/\/doi.org\/10.1073\/pnas.221575398","journal-title":"Proc Natl Acad Sci USA"},{"key":"1747_CR5","doi-asserted-by":"publisher","first-page":"1010","DOI":"10.1016\/j.micinf.2008.07.007","volume":"10","author":"J-P Gorvel","year":"2008","unstructured":"Gorvel J-P (2008) Brucella: a Mr Hide converted into Dr Jekyll. Microbes Infect 10:1010\u20131013","journal-title":"Microbes Infect"},{"key":"1747_CR6","doi-asserted-by":"publisher","first-page":"1015","DOI":"10.1128\/CMR.00016-17","volume":"30","author":"S Quainoo","year":"2017","unstructured":"Quainoo S, Coolen JPM, Van Hijum SAFT, Huynen MA, Melchers WJG, Van Schaik W, Wertheim HFL (2017) Whole-Genome sequencing of bacterial pathogens: the future of nosocomial outbreak analysis. Clin Microbiol Rev 30:1015\u20131063. https:\/\/doi.org\/10.1128\/CMR.00016-17","journal-title":"Clin Microbiol Rev"},{"key":"1747_CR7","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1007\/978-3-030-62155-1_4","volume-title":"Application and integration of Omics-powered diagnostics in clinical and public health microbiology","author":"F Tagini","year":"2021","unstructured":"Tagini F, Pillonel T, Greub G (2021) Whole-Genome sequencing for bacterial virulence assessment. In: Moran-Gilad J, Yagel Y (eds) Application and integration of Omics-powered diagnostics in clinical and public health microbiology. Springer International Publishing, Cham, pp 45\u201368"},{"key":"1747_CR8","doi-asserted-by":"publisher","first-page":"1482","DOI":"10.3389\/fmicb.2018.01482","volume":"9","author":"AW Pightling","year":"2018","unstructured":"Pightling AW, Pettengill JB, Luo Y, Baugher JD, Rand H, Strain E (2018) Interpreting whole-genome sequence analyses of foodborne bacteria for regulatory applications and outbreak investigations. Front Microbiol 9:1482","journal-title":"Front Microbiol"},{"key":"1747_CR9","doi-asserted-by":"publisher","first-page":"920","DOI":"10.1128\/JB.01091-13","volume":"196","author":"AR Wattam","year":"2014","unstructured":"Wattam AR, Foster JT, Mane SP, Beckstrom-Sternberg SM, Beckstrom-Sternberg JM, Dickerman AW, Keim P, Pearson T, Shukla M, Ward DV (2014) Comparative phylogenomics and evolution of the brucellae reveal a path to virulence. J Bacteriol 196:920\u2013930","journal-title":"J Bacteriol"},{"key":"1747_CR10","doi-asserted-by":"publisher","first-page":"2864","DOI":"10.1128\/JB.01581-08","volume":"191","author":"JT Foster","year":"2009","unstructured":"Foster JT, Beckstrom-Sternberg SM, Pearson T, Beckstrom-Sternberg JS, Chain PSG, Roberto FF, Hnath J, Brettin T, Keim P (2009) Whole-Genome-Based phylogeny and divergence of the genus Brucella. J Bacteriol 191:2864\u20132870. https:\/\/doi.org\/10.1128\/JB.01581-08","journal-title":"J Bacteriol"},{"key":"1747_CR11","doi-asserted-by":"publisher","first-page":"353","DOI":"10.1186\/s12864-018-4762-2","volume":"19","author":"SV Pisarenko","year":"2018","unstructured":"Pisarenko SV, Kovalev DA, Volynkina AS, Ponomarenko DG, Rusanova DV, Zharinova NV, Khachaturova AA, Tokareva LE, Khvoynova IG, Kulichenko AN (2018) Global evolution and phylogeography of Brucella melitensis strains. BMC Genomics 19:353. https:\/\/doi.org\/10.1186\/s12864-018-4762-2","journal-title":"BMC Genomics"},{"key":"1747_CR12","doi-asserted-by":"publisher","first-page":"996","DOI":"10.1038\/nbt.4229","volume":"36","author":"DH Parks","year":"2018","unstructured":"Parks DH, Chuvochina M, Waite DW, Rinke C, Skarshewski A, Chaumeil P-A, Hugenholtz P (2018) A standardized bacterial taxonomy based on genome phylogeny substantially revises the tree of life. Nat Biotechnol 36:996\u20131004","journal-title":"Nat Biotechnol"},{"key":"1747_CR13","doi-asserted-by":"publisher","first-page":"138","DOI":"10.1101\/gr.251678.119","volume":"30","author":"Z Zhou","year":"2020","unstructured":"Zhou Z, Alikhan N-F, Mohamed K, Fan Y, Achtman M, Brown D, Chattaway M, Dallman T, Delahay R, Kornschober C (2020) The enterobase user\u2019s guide, with case studies on Salmonella transmissions, Yersinia pestis phylogeny, and Escherichia core genomic diversity. Genome Res 30:138\u2013152","journal-title":"Genome Res"},{"key":"1747_CR14","doi-asserted-by":"publisher","first-page":"6968","DOI":"10.1038\/s41467-022-34630-w","volume":"13","author":"RC Edgar","year":"2022","unstructured":"Edgar RC (2022) Muscle5: High-accuracy alignment ensembles enable unbiased assessments of sequence homology and phylogeny. Nat Commun 13:6968","journal-title":"Nat Commun"},{"key":"1747_CR15","doi-asserted-by":"publisher","first-page":"350","DOI":"10.1016\/j.cmi.2017.12.016","volume":"24","author":"AC Sch\u00fcrch","year":"2018","unstructured":"Sch\u00fcrch AC, Arredondo-Alonso S, Willems RJL, Goering RV (2018) Whole genome sequencing options for bacterial strain typing and epidemiologic analysis based on single nucleotide polymorphism versus gene-by-gene\u2013based approaches. Clin Microbiol Infect 24:350\u2013354","journal-title":"Clin Microbiol Infect"},{"key":"1747_CR16","doi-asserted-by":"publisher","first-page":"980","DOI":"10.1111\/jvim.16678","volume":"37","author":"CC Kneipp","year":"2023","unstructured":"Kneipp CC, Deutscher AT, Coilparampil R, Rose AM, Robson J, Malik R, Stevenson MA, Wiethoelter AK, Mor SM (2023) Clinical investigation and management of Brucella suis seropositive dogs: A longitudinal case series. Veterinary Intern Medicne 37:980\u2013991. https:\/\/doi.org\/10.1111\/jvim.16678","journal-title":"Veterinary Intern Medicne"},{"key":"1747_CR17","doi-asserted-by":"publisher","first-page":"359","DOI":"10.3389\/fvets.2020.00359","volume":"7","author":"JF Lindahl","year":"2020","unstructured":"Lindahl JF, Chauhan A, Gill JPS, Hazarika RA, Fairoze NM, Grace D, Gaurav A, Satpathy SK, Kakkar M (2020) The extent and structure of peri-urban smallholder dairy farming in five cities in India. Front Veterinary Sci 7:359","journal-title":"Front Veterinary Sci"},{"key":"1747_CR18","unstructured":"Alton GG, Jones LM, Pietz DE, Organization WH (1975) Laboratory techniques in brucellosis. World Health Organization"},{"key":"1747_CR19","first-page":"271","volume":"95","author":"GG Baily","year":"1992","unstructured":"Baily GG, Krahn JB, Drasar BS, Stoker NG (1992) Detection of Brucella melitensis and Brucella abortus by DNA amplification. J Trop Med Hyg 95:271\u2013275","journal-title":"J Trop Med Hyg"},{"key":"1747_CR20","doi-asserted-by":"publisher","first-page":"2660","DOI":"10.1128\/jcm.32.11.2660-2666.1994","volume":"32","author":"BJ Bricker","year":"1994","unstructured":"Bricker BJ, Halling SM (1994) Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR. J Clin Microbiol 32:2660\u20132666. https:\/\/doi.org\/10.1128\/jcm.32.11.2660-2666.1994","journal-title":"J Clin Microbiol"},{"key":"1747_CR21","doi-asserted-by":"publisher","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","volume":"34","author":"S Chen","year":"2018","unstructured":"Chen S, Zhou Y, Chen Y, Gu J (2018) Fastp: an ultra-fast all-in-one FASTQ preprocessor. Bioinformatics 34:i884\u2013i890","journal-title":"Bioinformatics"},{"key":"1747_CR22","doi-asserted-by":"publisher","first-page":"e1005595","DOI":"10.1371\/journal.pcbi.1005595","volume":"13","author":"RR Wick","year":"2017","unstructured":"Wick RR, Judd LM, Gorrie CL, Holt KE (2017) Unicycler: resolving bacterial genome assemblies from short and long sequencing reads. PLoS Comput Biol 13:e1005595","journal-title":"PLoS Comput Biol"},{"key":"1747_CR23","doi-asserted-by":"publisher","first-page":"W537","DOI":"10.1093\/nar\/gky379","volume":"46","author":"E Afgan","year":"2018","unstructured":"Afgan E, Baker D, Batut B, van den Beek M, Bouvier D, \u010cech M, Chilton J, Clements D, Coraor N, Gr\u00fcning BA, Guerler A, Hillman-Jackson J, Hiltemann S, Jalili V, Rasche H, Soranzo N, Goecks J, Taylor J, Nekrutenko A, Blankenberg D (2018) The galaxy platform for accessible, reproducible and collaborative biomedical analyses: 2018 update. Nucleic Acids Res 46:W537\u2013W544. https:\/\/doi.org\/10.1093\/nar\/gky379","journal-title":"Nucleic Acids Res"},{"key":"1747_CR24","doi-asserted-by":"publisher","first-page":"5114","DOI":"10.1038\/s41467-018-07641-9","volume":"9","author":"C Jain","year":"2018","unstructured":"Jain C, Rodriguez-R LM, Phillippy AM, Konstantinidis KT, Aluru S (2018) High throughput ANI analysis of 90K prokaryotic genomes reveals clear species boundaries. Nat Commun 9:5114","journal-title":"Nat Commun"},{"key":"1747_CR25","doi-asserted-by":"publisher","first-page":"2443","DOI":"10.1093\/bioinformatics\/btv171","volume":"31","author":"E Bosi","year":"2015","unstructured":"Bosi E, Donati B, Galardini M, Brunetti S, Sagot M-F, Li\u00f3 P, Crescenzi P, Fani R, Fondi M (2015) MeDuSa: a multi-draft based scaffolder. Bioinformatics 31:2443\u20132451","journal-title":"Bioinformatics"},{"key":"1747_CR26","doi-asserted-by":"publisher","first-page":"6614","DOI":"10.1093\/nar\/gkw569","volume":"44","author":"T Tatusova","year":"2016","unstructured":"Tatusova T, DiCuccio M, Badretdin A, Chetvernin V, Nawrocki EP, Zaslavsky L, Lomsadze A, Pruitt KD, Borodovsky M, Ostell J (2016) NCBI prokaryotic genome annotation pipeline. Nucleic Acids Res 44:6614\u20136624","journal-title":"Nucleic Acids Res"},{"key":"1747_CR27","doi-asserted-by":"publisher","first-page":"1072","DOI":"10.1093\/bioinformatics\/btt086","volume":"29","author":"A Gurevich","year":"2013","unstructured":"Gurevich A, Saveliev V, Vyahhi N, Tesler G (2013) QUAST: quality assessment tool for genome assemblies. Bioinformatics 29:1072\u20131075","journal-title":"Bioinformatics"},{"key":"1747_CR28","unstructured":"Seemann T (2024) tseemann\/abricate"},{"key":"1747_CR29","doi-asserted-by":"publisher","first-page":"D325","DOI":"10.1093\/nar\/gki008","volume":"33","author":"L Chen","year":"2005","unstructured":"Chen L, Yang J, Yu J, Yao Z, Sun L, Shen Y, Jin Q (2005) VFDB: a reference database for bacterial virulence factors. Nucleic Acids Res 33:D325\u2013D328","journal-title":"Nucleic Acids Res"},{"key":"1747_CR30","doi-asserted-by":"publisher","first-page":"3348","DOI":"10.1128\/AAC.00419-13","volume":"57","author":"AG McArthur","year":"2013","unstructured":"McArthur AG, Waglechner N, Nizam F, Yan A, Azad MA, Baylay AJ, Bhullar K, Canova MJ, De Pascale G, Ejim L, Kalan L, King AM, Koteva K, Morar M, Mulvey MR, O\u2019Brien JS, Pawlowski AC, Piddock LJV, Spanogiannopoulos P, Sutherland AD, Tang I, Taylor PL, Thaker M, Wang W, Yan M, Yu T, Wright GD (2013) The comprehensive antibiotic resistance database. Antimicrob Agents Chemother 57:3348\u20133357. https:\/\/doi.org\/10.1128\/AAC.00419-13","journal-title":"Antimicrob Agents Chemother"},{"key":"1747_CR31","doi-asserted-by":"publisher","first-page":"2068","DOI":"10.1093\/bioinformatics\/btu153","volume":"30","author":"T Seemann","year":"2014","unstructured":"Seemann T (2014) Prokka: rapid prokaryotic genome annotation. Bioinformatics 30:2068\u20132069","journal-title":"Bioinformatics"},{"key":"1747_CR32","doi-asserted-by":"publisher","first-page":"180","DOI":"10.1186\/s13059-020-02090-4","volume":"21","author":"G Tonkin-Hill","year":"2020","unstructured":"Tonkin-Hill G, MacAlasdair N, Ruis C, Weimann A, Horesh G, Lees JA, Gladstone RA, Lo S, Beaudoin C, Floto RA, Frost SDW, Corander J, Bentley SD, Parkhill J (2020) Producing polished prokaryotic pangenomes with the Panaroo pipeline. Genome Biol 21:180. https:\/\/doi.org\/10.1186\/s13059-020-02090-4","journal-title":"Genome Biol"},{"key":"1747_CR33","doi-asserted-by":"publisher","first-page":"1297","DOI":"10.1093\/bioinformatics\/btu017","volume":"30","author":"Y Zhao","year":"2014","unstructured":"Zhao Y, Jia X, Yang J, Ling Y, Zhang Z, Yu J, Wu J, Xiao J (2014) PanGP: a tool for quickly analyzing bacterial pan-genome profile. Bioinformatics 30:1297\u20131299","journal-title":"Bioinformatics"},{"key":"1747_CR34","doi-asserted-by":"publisher","first-page":"524","DOI":"10.1186\/s13059-014-0524-x","volume":"15","author":"TJ Treangen","year":"2014","unstructured":"Treangen TJ, Ondov BD, Koren S, Phillippy AM (2014) The harvest suite for rapid core-genome alignment and visualization of thousands of intraspecific microbial genomes. Genome Biol 15:524. https:\/\/doi.org\/10.1186\/s13059-014-0524-x","journal-title":"Genome Biol"},{"key":"1747_CR35","unstructured":"Seemann T (2024) tseemann\/snp-dists"},{"key":"1747_CR36","doi-asserted-by":"publisher","first-page":"4453","DOI":"10.1093\/bioinformatics\/btz305","volume":"35","author":"AM Kozlov","year":"2019","unstructured":"Kozlov AM, Darriba D, Flouri T, Morel B, Stamatakis A (2019) RAxML-NG: a fast, scalable and user-friendly tool for maximum likelihood phylogenetic inference. Bioinformatics 35:4453\u20134455","journal-title":"Bioinformatics"},{"key":"1747_CR37","doi-asserted-by":"publisher","first-page":"W242","DOI":"10.1093\/nar\/gkw290","volume":"44","author":"I Letunic","year":"2016","unstructured":"Letunic I, Bork P (2016) Interactive tree of life (iTOL) v3: an online tool for the display and annotation of phylogenetic and other trees. Nucleic Acids Res 44:W242\u2013W245","journal-title":"Nucleic Acids Res"},{"key":"1747_CR38","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1186\/1471-2180-7-34","volume":"7","author":"AM Whatmore","year":"2007","unstructured":"Whatmore AM, Perrett LL, MacMillan AP (2007) Characterisation of the genetic diversity of Brucella by multilocus sequencing. BMC Microbiol 7:34. https:\/\/doi.org\/10.1186\/1471-2180-7-34","journal-title":"BMC Microbiol"},{"key":"1747_CR39","doi-asserted-by":"publisher","first-page":"2049","DOI":"10.3389\/fmicb.2016.02049","volume":"7","author":"AM Whatmore","year":"2016","unstructured":"Whatmore AM, Koylass MS, Muchowski J, Edwards-Smallbone J, Gopaul KK, Perrett LL (2016) Extended multilocus sequence analysis to describe the global population structure of the genus brucella: phylogeography and relationship to Biovars. Front Microbiol 7:2049","journal-title":"Front Microbiol"},{"key":"1747_CR40","doi-asserted-by":"publisher","first-page":"e00311","DOI":"10.1128\/jcm.00311-22","volume":"60","author":"MY Abdel-Glil","year":"2022","unstructured":"Abdel-Glil MY, Thomas P, Brandt C, Melzer F, Subbaiyan A, Chaudhuri P, Harmsen D, Jolley KA, Janowicz A, Garofolo G, Neubauer H, Pletz MW (2022) Core genome multilocus sequence typing scheme for improved characterization and epidemiological surveillance of pathogenic Brucella. J Clin Microbiol 60:e00311\u2013e00322. https:\/\/doi.org\/10.1128\/jcm.00311-22","journal-title":"J Clin Microbiol"},{"key":"1747_CR41","unstructured":"cgMLST.org Nomenclature Server (h25). https:\/\/www.cgmlst.org\/ncs. Accessed 27 Jun 2024"},{"key":"1747_CR42","unstructured":"bvalot (2024) bvalot\/pyMLST"},{"key":"1747_CR43","doi-asserted-by":"crossref","unstructured":"Zhou Z, Alikhan NF, Sergeant MJ, Luhmann N, Vaz C, Francisco AP, Carri\u00e7o JA, Achtman M (2018) GrapeTree: visualization of core genomic relationships among 100,000 bacterial pathogens. Genome Res 28: 1395-1404","DOI":"10.1101\/gr.232397.117"},{"key":"1747_CR44","doi-asserted-by":"publisher","first-page":"1","DOI":"10.2147\/VMRR.S91360","volume":"8","author":"S Olsen","year":"2016","unstructured":"Olsen S, Tatum F (2016) Swine brucellosis: current perspectives. VMRR Volume 8:1\u201312. https:\/\/doi.org\/10.2147\/VMRR.S91360","journal-title":"VMRR Volume"},{"key":"1747_CR45","doi-asserted-by":"publisher","first-page":"124","DOI":"10.1016\/j.cimid.2019.05.002","volume":"65","author":"R Shome","year":"2019","unstructured":"Shome R, Triveni K, Swati S, Ranjitha S, Krithiga N, Shome BR, Nagalingam M, Rahman H, Barbuddhe SB (2019) Spatial Seroprevalence of bovine brucellosis in India\u2014A large random sampling survey. Comp Immunol Microbiol Infect Dis 65:124\u2013127","journal-title":"Comp Immunol Microbiol Infect Dis"},{"key":"1747_CR46","unstructured":"Szulowski K, Iwaniak W, Weiner M, Zlotnicka J (2013) Brucella suis biovar 2 isolations from cattle in Poland. Ann Agric Environ Med 20"},{"key":"1747_CR47","doi-asserted-by":"crossref","unstructured":"Cook DR, Noble JW (1984) Isolation of Brucella suis from cattle","DOI":"10.1111\/j.1751-0813.1984.tb15539.x"},{"key":"1747_CR48","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1177\/104063879700900414","volume":"9","author":"DR Ewalt","year":"1997","unstructured":"Ewalt DR, Payeur JB, Rhyan JC, Geer PL (1997) Brucella Suis biovar 1 in naturally infected cattle: A bacteriological, serological, and histological study. J VET Diagn Invest 9:417\u2013420. https:\/\/doi.org\/10.1177\/104063879700900414","journal-title":"J VET Diagn Invest"},{"key":"1747_CR49","doi-asserted-by":"publisher","first-page":"442","DOI":"10.1016\/j.vetmic.2014.01.039","volume":"170","author":"MS Kin","year":"2014","unstructured":"Kin MS, Fort M, de Echaide ST, Casanave EB (2014) Brucella suis in armadillos (Chaetophractus villosus) from La pampa, Argentina. Vet Microbiol 170:442\u2013445","journal-title":"Vet Microbiol"},{"key":"1747_CR50","doi-asserted-by":"crossref","unstructured":"Paolicchi FA, Terzolo HR, Campero CM (1993) Isolation of Brucella suis from the semen of a ram","DOI":"10.1136\/vr.132.3.67"},{"key":"1747_CR51","doi-asserted-by":"publisher","first-page":"2386","DOI":"10.3201\/eid1712.111127","volume":"17","author":"S Ramamoorthy","year":"2011","unstructured":"Ramamoorthy S, Woldemeskel M, Ligett A, Snider R, Cobb R, Rajeev S (2011) Brucella suis infection in dogs, georgia, USA. Emerg Infect Dis 17:2386","journal-title":"Emerg Infect Dis"},{"key":"1747_CR52","doi-asserted-by":"publisher","first-page":"584","DOI":"10.1136\/vr.156.18.584","volume":"156","author":"Z Cvetnic","year":"2005","unstructured":"Cvetnic Z, Spicic S, Curic S, Jukic B, Lojkic M, Albert D, Thi\u00e9baud M, Garin-Bastuji B (2005) Isolation of Brucella suis biovar 3 from horses in Croatia. Veterinary Record-English Ed 156:584\u2013585","journal-title":"Veterinary Record-English Ed"},{"key":"1747_CR53","doi-asserted-by":"publisher","first-page":"e21","DOI":"10.1371\/journal.ppat.0040021","volume":"4","author":"SP Salcedo","year":"2008","unstructured":"Salcedo SP, Marchesini MI, Lelouard H, Fugier E, Jolly G, Balor S, Muller A, Lapaque N, Demaria O, Alexopoulou L (2008) Brucella control of dendritic cell maturation is dependent on the TIR-containing protein Btp1. PLoS Pathog 4:e21","journal-title":"PLoS Pathog"},{"key":"1747_CR54","doi-asserted-by":"publisher","first-page":"113557","DOI":"10.1016\/j.biopha.2022.113557","volume":"155","author":"H Tarrahimofrad","year":"2022","unstructured":"Tarrahimofrad H, Zamani J, Hamblin MR, Darvish M, Mirzaei H (2022) A designed peptide-based vaccine to combat Brucella melitensis, B. suis and B. abortus: Harnessing an epitope mapping and immunoinformatics approach. Biomed Pharmacother 155:113557","journal-title":"Biomed Pharmacother"},{"key":"1747_CR55","doi-asserted-by":"publisher","first-page":"1860","DOI":"10.1128\/JB.06561-11","volume":"194","author":"M Mancilla","year":"2012","unstructured":"Mancilla M, Mar\u00edn CM, Blasco JM, Z\u00e1rraga AM, L\u00f3pez-Go\u00f1i I, Moriy\u00f3n I (2012) Spontaneous excision of the O-Polysaccharide WbkA glycosyltranferase gene is a cause of dissociation of smooth to rough Brucella colonies. J Bacteriol 194:1860\u20131867. https:\/\/doi.org\/10.1128\/JB.06561-11","journal-title":"J Bacteriol"},{"key":"1747_CR56","doi-asserted-by":"publisher","first-page":"655","DOI":"10.1016\/S0923-2508(00)90130-X","volume":"151","author":"F Godfroid","year":"2000","unstructured":"Godfroid F, Cloeckaert A, Taminiau B, Danese I, Tibor A, De Bolle X, Mertens P, Letesson J-J (2000) Genetic organisation of the lipopolysaccharide O-antigen biosynthesis region of Brucella melitensis 16\u00a0M (wbk). Res Microbiol 151:655\u2013668","journal-title":"Res Microbiol"},{"key":"1747_CR57","doi-asserted-by":"publisher","first-page":"9341","DOI":"10.3390\/ijms21249341","volume":"21","author":"D Kornspan","year":"2020","unstructured":"Kornspan D, Lubkovskaia R, Mathur S, Yeheskel A, Salmon-Divon M (2020) Genomic analysis of natural rough Brucella melitensis rev. 1 vaccine strains: identification and characterization of mutations in key genes associated with bacterial Lps biosynthesis and virulence. Int J Mol Sci 21:9341","journal-title":"Int J Mol Sci"},{"key":"1747_CR58","doi-asserted-by":"publisher","first-page":"2530","DOI":"10.1128\/JB.01198-08","volume":"191","author":"FA Martin","year":"2009","unstructured":"Martin FA, Posadas DM, Carrica MC, Cravero SL, O\u2019Callaghan D, Zorreguieta A (2009) Interplay between two RND systems mediating antimicrobial resistance in Brucella suis. J Bacteriol 191:2530\u20132540. https:\/\/doi.org\/10.1128\/JB.01198-08","journal-title":"J Bacteriol"},{"key":"1747_CR59","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1016\/j.meegid.2016.04.012","volume":"41","author":"J Sankarasubramanian","year":"2016","unstructured":"Sankarasubramanian J, Vishnu US, Gunasekaran P, Rajendhran J (2016) A genome-wide SNP-based phylogenetic analysis distinguishes different Biovars of Brucella suis. Infect Genet Evol 41:213\u2013217","journal-title":"Infect Genet Evol"},{"key":"1747_CR60","doi-asserted-by":"publisher","first-page":"336","DOI":"10.3390\/microorganisms10020336","volume":"10","author":"G Girault","year":"2022","unstructured":"Girault G, Perrot L, Mick V, Ponsart C (2022) High-resolution melting PCR as rapid genotyping tool for Brucella species. Microorganisms 10:336","journal-title":"Microorganisms"},{"key":"1747_CR61","doi-asserted-by":"publisher","first-page":"88","DOI":"10.1007\/s12088-014-0486-4","volume":"55","author":"J Sankarasubramanian","year":"2015","unstructured":"Sankarasubramanian J, Vishnu US, Sridhar J, Gunasekaran P, Rajendhran J (2015) Pan-Genome of Brucella species. Indian J Microbiol 55:88\u2013101. https:\/\/doi.org\/10.1007\/s12088-014-0486-4","journal-title":"Indian J Microbiol"},{"key":"1747_CR62","doi-asserted-by":"publisher","first-page":"1584","DOI":"10.3390\/microorganisms10081584","volume":"10","author":"B Kurmanov","year":"2022","unstructured":"Kurmanov B, Zincke D, Su W, Hadfield TL, Aikimbayev A, Karibayev T, Berdikulov M, Orynbayev M, Nikolich MP, Blackburn JK (2022) Assays for identification and differentiation of Brucella species: a review. Microorganisms 10:1584","journal-title":"Microorganisms"},{"key":"1747_CR63","doi-asserted-by":"publisher","first-page":"7749","DOI":"10.3390\/ijms21207749","volume":"21","author":"M Su\u00e1rez-Esquivel","year":"2020","unstructured":"Su\u00e1rez-Esquivel M, Chaves-Olarte E, Moreno E, Guzm\u00e1n-Verri C (2020) Brucella genomics: macro and micro evolution. Int J Mol Sci 21:7749","journal-title":"Int J Mol Sci"},{"key":"1747_CR64","unstructured":"In\u00e1cio ACRAF (2017) Comparative genomics in Brucella suis: from intra-specific and inter-specific distinctive features to diagnostic molecular markers. PhD Thesis, Universidade de Lisboa (Portugal)"},{"key":"1747_CR65","doi-asserted-by":"publisher","first-page":"1233118","DOI":"10.3389\/fvets.2023.1233118","volume":"10","author":"S Aurich","year":"2023","unstructured":"Aurich S, Schneider J, Brangsch H, Koets A, Melzer F, Ewers C, Prenger-Berninghoff E (2023) Brucella suis biovar 1 infection in a dog with orchitis in Germany. Front Veterinary Sci 10:1233118","journal-title":"Front Veterinary Sci"}],"container-title":["Brazilian Journal of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s42770-025-01747-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s42770-025-01747-1\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s42770-025-01747-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T11:14:40Z","timestamp":1764242080000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s42770-025-01747-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,6]]},"references-count":65,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["1747"],"URL":"https:\/\/doi.org\/10.1007\/s42770-025-01747-1","relation":{},"ISSN":["1517-8382","1678-4405"],"issn-type":[{"type":"print","value":"1517-8382"},{"type":"electronic","value":"1678-4405"}],"subject":[],"published":{"date-parts":[[2025,8,6]]},"assertion":[{"value":"17 February 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 July 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 August 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors have no competing interests to declare that are relevant to the content of this article.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}