{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T11:20:59Z","timestamp":1764242459707,"version":"3.46.0"},"reference-count":38,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T00:00:00Z","timestamp":1758758400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T00:00:00Z","timestamp":1758758400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2021\/13556-3"],"award-info":[{"award-number":["2021\/13556-3"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Braz J Microbiol"],"published-print":{"date-parts":[[2025,12]]},"DOI":"10.1007\/s42770-025-01779-7","type":"journal-article","created":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T19:11:40Z","timestamp":1758827500000},"page":"3085-3091","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparison of qPCR methods for the diagnosis of bovine tuberculosis from granulomas collected at slaughterhouses"],"prefix":"10.1007","volume":"56","author":[{"given":"Juliana Fernandes","family":"de Paula Castro","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gisele Oliveira","family":"de Souza","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sueli Akemi","family":"Taniwaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcos Bryan","family":"Heinemann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1286-6245","authenticated-orcid":false,"given":"Jos\u00e9 Soares","family":"Ferreira Neto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,9,25]]},"reference":[{"key":"1779_CR1","doi-asserted-by":"publisher","first-page":"3793","DOI":"10.5433\/1679-0359.2016v37n5Supl2p3793","volume":"37","author":"VSF Homem","year":"2016","unstructured":"Homem VSF, Higa ZMM, Ferreira Neto JS (2016) Proposed model to study the economic impact of bovine brucellosis and tuberculosis: case study of pirassununga, SP, Brazil. Semin Ci\u00eanc Agr\u00e1r 37:3793\u20133802. https:\/\/doi.org\/10.5433\/1679-0359.2016v37n5Supl2p3793","journal-title":"Semin Ci\u00eanc Agr\u00e1r"},{"key":"1779_CR2","doi-asserted-by":"publisher","first-page":"360","DOI":"10.1007\/s11250-022-03350-0","volume":"54","author":"DL Rodrigues","year":"2022","unstructured":"Rodrigues DL, Amorim EA, Ferreira F, Amaku M, Baquero OS, Grisi Filho JHH, Dias RA, Heinemann MB, Telles EO, Gon\u00e7alves VSG, Compton C, Ferreira Neto JS (2022) Apparent prevalence and risk factors for bovine tuberculosis in the state of paran\u00e1, brazil: an assessment after 18 years since the beginning of the Brazilian program. Trop Anim Health Prod 54:360. https:\/\/doi.org\/10.1007\/s11250-022-03350-0","journal-title":"Trop Anim Health Prod"},{"key":"1779_CR3","first-page":"512","volume-title":"Doen\u00e7as infecciosas Em animais de produ\u00e7\u00e3o e de companhia, 1\u00aa edi\u00e7\u00e3o","author":"AC Paes","year":"2016","unstructured":"Paes AC, Franco MMJ (2016) Tuberculose Em animais de produ\u00e7\u00e3o. In: Megid J, Ribeiro MG, Paes AC (eds) Doen\u00e7as infecciosas Em animais de produ\u00e7\u00e3o e de companhia, 1\u00aa edi\u00e7\u00e3o. Roca, Rio de Janeiro, pp 512\u2013542"},{"key":"1779_CR4","volume-title":"Programa Nacional de controle e erradica\u00e7\u00e3o Da brucelose e Da tuberculose animal (PNCEBT). Manual t\u00e9cnico","author":"AP Lage","year":"2006","unstructured":"Lage AP, Roxo E, M\u00fcller EE, Poester FP, Cavall\u00e9ro JCM, Ferreira Neto JS, Mota PMPC, Gon\u00e7alves VSP (2006) Programa Nacional de controle e erradica\u00e7\u00e3o Da brucelose e Da tuberculose animal (PNCEBT). Manual t\u00e9cnico. Minist\u00e9rio da Agricultura, Pecu\u00e1ria e Abastecimento, Bras\u00edlia"},{"key":"1779_CR5","doi-asserted-by":"publisher","first-page":"3549","DOI":"10.5433\/1679-0359.2016v37n5Supl2p3549","volume":"37","author":"L Bahiense","year":"2016","unstructured":"Bahiense L, \u00c1vila LN, Bavia ME, Amaku M, Dias RA, Grisi Filho JHH, Ferreira F, Telles EO, Gon\u00e7alves VPS, Heinemann MB, Ferreira Neto JS (2016) Prevalence and risk factors for bovine tuberculosis in the state of bahia, Brazil. Semin Ci\u00eanc Agr\u00e1r 37:3549\u20133560. https:\/\/doi.org\/10.5433\/1679-0359.2016v37n5Supl2p3549","journal-title":"Semin Ci\u00eanc Agr\u00e1r"},{"key":"1779_CR6","doi-asserted-by":"publisher","first-page":"3531","DOI":"10.5433\/1679-0359.2016v37n5Supl2p3531","volume":"37","author":"JDM Barbieri","year":"2016","unstructured":"Barbieri JDM, Oliveira LF, Dorneles EMS, Mota ALAAA, Gon\u00e7aves VSP, Maluf PP, Ferreira Neto JS, Ferreira F, Dias RA, Telles EO, Grisi Filho JHH, Heinemann MB, Amaku M, Lage AP (2016) Epidemiological status of bovine tuberculosis in the state of Minas gerais, Brazil. Semin Ci\u00eanc Agr\u00e1r 37:3531\u20133548. https:\/\/doi.org\/10.5433\/1679-0359.2016v37n5Supl2p3531","journal-title":"Semin Ci\u00eanc Agr\u00e1r"},{"key":"1779_CR7","doi-asserted-by":"publisher","first-page":"3673","DOI":"10.5433\/1679-0359.2016v37n5Supl2p3673","volume":"37","author":"RA Dias","year":"2016","unstructured":"Dias RA, Stanojlovic FMU, Belchior APC, Ferreira RS, Gon\u00e7alves RC, Bar\u00e3o RSC, Sousa PR, Santos AMA, Amaku M, Ferreira F, Telles EO, Grisi Filho JHH, Gon\u00e7alves VSP, Heinemann MB, Ferreira Neto JS (2016) Prevalence and risk factors for bovine tuberculosis in the state of S\u00e3o paulo, Brazil. Semin Ci\u00eanc Agr\u00e1r 37:3673\u20133684. https:\/\/doi.org\/10.5433\/1679-0359.2016v37n5Supl2p3673","journal-title":"Semin Ci\u00eanc Agr\u00e1r"},{"key":"1779_CR8","doi-asserted-by":"publisher","first-page":"1673","DOI":"10.5433\/1679-0359.2021v42n3Supl1p1673","volume":"42","author":"JS Ferreira Neto","year":"2021","unstructured":"Ferreira Neto JS, Barbosa RG, Ferreira F, Amaku M, Dias RA, Grisi Filho JHH, Gon\u00e7alves VSP, Baquero OS, Machado ST, Telles EO (2021) Epidemilogical situation of bovine tuberculosis in the state of tocantins, Brazil. Semin Ci\u00eanc Agr\u00e1r 42:1673\u20131684. https:\/\/doi.org\/10.5433\/1679-0359.2021v42n3Supl1p1673","journal-title":"Semin Ci\u00eanc Agr\u00e1r"},{"key":"1779_CR9","doi-asserted-by":"publisher","first-page":"3567","DOI":"10.5433\/1679-0359.2016v37n5Supl2p3567","volume":"37","author":"JOA Galvis","year":"2016","unstructured":"Galvis JOA, Grisi Filho JHH, Costa D, Said ALPR, Amaku M, Dias RA, Ferreira F, Gon\u00e7alves VSP, Heinemann MB, Telles EO, Ferreira Neto JS (2016) Epidemiologic characterization of bovine tuberculosis in the state of Esp\u00edrito santo, Brazil. Semin Ci\u00eanc Agr\u00e1r 37:3567\u20133578. https:\/\/doi.org\/10.5433\/1679-0359.2016v37n5Supl2p3567","journal-title":"Semin Ci\u00eanc Agr\u00e1r"},{"key":"1779_CR10","doi-asserted-by":"publisher","first-page":"3579","DOI":"10.5433\/1679-0359.2016v37n5Supl2p3579","volume":"37","author":"IB Guedes","year":"2016","unstructured":"Guedes IB, Bottene IFN, Monteiro LARC, Leal Filho JM, Heinemann MB, Amaku M, Grisi Filho JHH, Dias RA, Ferreira F, Telles EO, Gon\u00e7alves VSP, Ferreira Neto JS (2016) Prevalence and risk factors for bovine tuberculosis in the state of Mato Grosso do sul, Brazil. Semin Ci\u00eanc Agr\u00e1r 37:3579\u20133588. https:\/\/doi.org\/10.5433\/1679-0359.2016v37n5Supl2p3579","journal-title":"Semin Ci\u00eanc Agr\u00e1r"},{"key":"1779_CR11","doi-asserted-by":"publisher","first-page":"3601","DOI":"10.5433\/1679-0359.2016v37n5Supl2p3601","volume":"37","author":"PRB Lima","year":"2016","unstructured":"Lima PRB, Nascimento DL, Almeida EC, Pontual KAQ, Amaku M, Dias RA, Ferreira F, Gon\u00e7alves VSP, Telles EO, Grisi Filho JHH, Heinemann MB, Silva JCR, Ferreira Neto JS (2016) Epidemiological situation of bovine tuberculosis in the state of pernambuco, Brazil. Semin Ci\u00eanc Agr\u00e1r 37:3601\u20133610. https:\/\/doi.org\/10.5433\/1679-0359.2016v37n5Supl2p3601","journal-title":"Semin Ci\u00eanc Agr\u00e1r"},{"key":"1779_CR12","doi-asserted-by":"publisher","first-page":"3589","DOI":"10.5433\/1679-0359.2016v37n5Supl2p3589","volume":"37","author":"JMB N\u00e9spoli","year":"2016","unstructured":"N\u00e9spoli JMB, Negreiros RL, Amaku M, Dias RA, Ferreira F, Telles EO, Heinemann MB, Grisi Filho JHH, Gon\u00e7alves VSP, Ferreira Neto JS (2016) Epidemiological situation of bovine tuberculosis in the state of Mato grosso, Brazil. Semin Ci\u00eanc Agr\u00e1r 37:3589\u20133600. https:\/\/doi.org\/10.5433\/1679-0359.2016v37n5Supl2p3589","journal-title":"Semin Ci\u00eanc Agr\u00e1r"},{"key":"1779_CR13","doi-asserted-by":"publisher","first-page":"1466199","DOI":"10.3389\/fvets.2024.1466199","volume":"11","author":"BCRS Oliveira","year":"2024","unstructured":"Oliveira BCRS, Oliveira JP, Pinho APVB, Dias RA, Grisi Filho JHH, Gon\u00e7alves VSP, Heinemann MB, Amaku M, Telles EO, Ferreira BFS, Ferreira F, Ferreira Neto JS (2024) Epidemiological situation of bovine and Bubaline tuberculosis in the state of par\u00e1, Amazon region of Brazil. Front Vet Sci 11:1466199. https:\/\/doi.org\/10.3389\/fvets.2024.1466199","journal-title":"Front Vet Sci"},{"key":"1779_CR14","doi-asserted-by":"publisher","first-page":"3647","DOI":"10.5433\/1679-0359.2016v37n5Supl2p3647","volume":"37","author":"MR Queiroz","year":"2016","unstructured":"Queiroz MR, Groff ACM, Silva NDS, Grisi Filho JHH, Amaku M, Dias RA, Telles EO, Heinemann MB, Ferreira Neto JS, Gon\u00e7alves VSP, Ferreira F (2016) Epidemiological status of bovine tuberculosis in the state of Rio Grande do sul, Brazil. Semin Ci\u00eanc Agr\u00e1r 37:3647\u20133658. https:\/\/doi.org\/10.5433\/1679-0359.2016v37n5Supl2p3647","journal-title":"Semin Ci\u00eanc Agr\u00e1r"},{"key":"1779_CR15","doi-asserted-by":"publisher","first-page":"3561","DOI":"10.5433\/1679-0359.2016v37n5Supl2p3561","volume":"37","author":"LA Ribeiro","year":"2016","unstructured":"Ribeiro LA, Gon\u00e7alves VSP, Francisco PFC, Mota ALAA, Nascimento GT, Licurgo JB, Ferreira F, Grisi Filho JHH, Ferreira Neto JS, Amaku M, Dias RA, Telles EO, Heinemann MB, Borges JRJ (2016) Epidemiological status of bovine tuberculosis in the federal district of Brazil. Semin Ci\u00eanc Agr\u00e1r 37:3561\u20133566. https:\/\/doi.org\/10.5433\/1679-0359.2016v37n5Supl2p3561","journal-title":"Semin Ci\u00eanc Agr\u00e1r"},{"key":"1779_CR16","doi-asserted-by":"publisher","first-page":"3625","DOI":"10.5433\/1679-0359.2016v37n5Supl2p3625","volume":"37","author":"WV Rocha","year":"2016","unstructured":"Rocha WV, Jayme VS, Mota ALAA, Brito WMED, Pires GRC, Ferreira Neto JS, Grisi Filho JHH, Dias RA, Amaku M, Telles EO, Ferreira F, Heinemann MB, Gon\u00e7alves VSP (2016) Prevalence and herd-level risk factors of bovine tuberculosis in the state of goi\u00e1s, Brazil. Semin Ci\u00eanc Agr\u00e1r 37:3625\u20133638. https:\/\/doi.org\/10.5433\/1679-0359.2016v37n5Supl2p3625","journal-title":"Semin Ci\u00eanc Agr\u00e1r"},{"key":"1779_CR17","doi-asserted-by":"publisher","first-page":"3659","DOI":"10.5433\/1679-0359.2016v37n5Supl2p3659","volume":"37","author":"FP Veloso","year":"2016","unstructured":"Veloso FP, Baumgartem KD, Mota AAA, Ferreira F, Ferreira Neto JS, Grisi Filho JHH, Dias RA, Amaku M, Telles EO, Gon\u00e7alves VSP (2016) Prevalence and herd-level risk factors of bovine tuberculosis in the state of Santa Catarina. Semin Ci\u00eanc Agr\u00e1r 37:3659\u20133672. https:\/\/doi.org\/10.5433\/1679-0359.2016v37n5Supl2p3659","journal-title":"Semin Ci\u00eanc Agr\u00e1r"},{"key":"1779_CR18","doi-asserted-by":"publisher","first-page":"3639","DOI":"10.5433\/1679-0359.2016v37n5Supl2p3639","volume":"37","author":"FB Vendrame","year":"2016","unstructured":"Vendrame FB, Amaku M, Ferreira F, Telles EO, Grisi Filho JHH, Gon\u00e7alves VSP, Heinemann MB, Ferreira Neto JS, Dias RA (2016) Epidemiologic characterization of bovine tuberculosis in the state of rond\u00f4nia, Brazil. Semin Ci\u00eanc Agr\u00e1r 37:3639\u20133646. https:\/\/doi.org\/10.5433\/1679-0359.2016v37n5Supl2p3639","journal-title":"Semin Ci\u00eanc Agr\u00e1r"},{"key":"1779_CR19","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1590\/1808-1657v78p1752011","volume":"78","author":"JHH Grisi Filho","year":"2011","unstructured":"Grisi Filho JHH, Rosales CAR, Ferreira F, Amaku M, Dias RA, Ferreira Neto JS (2011) An\u00e1lise epidemiol\u00f3gica Das Condena\u00e7\u00f5es de Bovinos Por tuberculose Em abatedouros do Estado de S\u00e3o Paulo. Arq Inst Biol 78:175\u2013181. https:\/\/doi.org\/10.1590\/1808-1657v78p1752011","journal-title":"Arq Inst Biol"},{"key":"1779_CR20","doi-asserted-by":"publisher","unstructured":"Ferreira Neto JS, Silveira GBS, Rosa BM, Gon\u00e7alves VSP, Grisi Filho JHH, Amaku M, Dias RA, Ferreira F, Heinemann MB, Telles EO, Lage AP (2016) Analysis of 15 years of the National Program for the Control and Eradication of Animal Brucellosis and Tuberculosis, Brazil. Semin, Ci\u00eanc Agr\u00e1r 37:3385\u20133402, 2016. https:\/\/doi.org\/10.5433\/1679-0359.2016v37n5Supl2p3385","DOI":"10.5433\/1679-0359.2016v37n5Supl2p3385"},{"key":"1779_CR21","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1186\/1746-6148-3-12","volume":"3","author":"GM Taylor","year":"2007","unstructured":"Taylor GM, Worth DR, Palmer S, Jahans K, Hewinson RG (2007) Rapid detection of Mycobacterium Bovis DNA in cattle lymphnodes with visible lesions using PCR. Braz J Microbiol 3:12. https:\/\/doi.org\/10.1186\/1746-6148-3-12","journal-title":"Braz J Microbiol"},{"key":"1779_CR22","doi-asserted-by":"publisher","first-page":"73","DOI":"10.3329\/jbau.v9i1.8747","volume":"9","author":"Q Nahar","year":"2011","unstructured":"Nahar Q, Pervin MT, Islam MT, Khan MAHNA (2011) Application of PCR for the detection of bovine tuberculosis in cattle. J Bangladesh Agric Univ 9:73\u201378. https:\/\/doi.org\/10.3329\/jbau.v9i1.8747","journal-title":"J Bangladesh Agric Univ"},{"key":"1779_CR23","doi-asserted-by":"publisher","first-page":"e91023","DOI":"10.1371\/journal.pone.0091023","volume":"9","author":"CP Ara\u00fajo","year":"2014","unstructured":"Ara\u00fajo CP, Os\u00f3rio ALAR, Jorge KSG, Ramos CAN, Souza Filho AF, Vidal CES, Roxo E, Nishibe C, Almeida NF, J\u00fanior AAF, Silva MR, Neto JDB, Cerqueira VD, Zum\u00e1rraga MJ, Ara\u00fajo FR (2014) Detection of Mycobacterium Bovis in bovine and Bubaline tissues using Nested-PCR for TbD1. PLoS ONE 9:e91023. https:\/\/doi.org\/10.1371\/journal.pone.0091023","journal-title":"PLoS ONE"},{"key":"1779_CR24","doi-asserted-by":"publisher","first-page":"1223","DOI":"10.1590\/0103-8478cr20151489","volume":"46","author":"A Moura","year":"2016","unstructured":"Moura A, Hodon MA, Soares Filho PM, Issa MA, Oliveira APF, Fonseca Junior AA (2016) Comparison of nine DNA extraction methods for the diagnosis of bovine tuberculosis by real time PCR. Semin Ci\u00eanc Agr\u00e1r 46:1223\u20131228. https:\/\/doi.org\/10.1590\/0103-8478cr20151489","journal-title":"Semin Ci\u00eanc Agr\u00e1r"},{"key":"1779_CR25","doi-asserted-by":"publisher","first-page":"e1703","DOI":"10.7287\/peerj.preprints.1703v1","volume":"4","author":"PE Dykema","year":"2016","unstructured":"Dykema PE, Stokes KD, Beckwith NR, Mungin JW, Xu L, Vickers DJ, Reising MM, Bravo DM, Thomsen BV, Robbeausterman S (2016) Development and validation of a direct real-time PCR assay for Mycobacterium Bovis and implementation into the united States National surveillance program. Peer J PrePrints 4:e1703. https:\/\/doi.org\/10.7287\/peerj.preprints.1703v1","journal-title":"Peer J PrePrints"},{"key":"1779_CR26","doi-asserted-by":"publisher","first-page":"61","DOI":"10.3389\/fvets.2019.00061","volume":"6","author":"VL Leal","year":"2019","unstructured":"Leal VL, Liandris E, Castellanos E, Bezos J, Dom\u00ednguez L, De Juan L, Romero B (2019) Validation of a Real-Time PCR for the detection of Mycobacterium tuberculosis complex members in bovine tissue samples. Front Vet Sci 6:61. https:\/\/doi.org\/10.3389\/fvets.2019.00061","journal-title":"Front Vet Sci"},{"key":"1779_CR27","doi-asserted-by":"publisher","first-page":"643111","DOI":"10.3389\/fvets.2021.643111","volume":"8","author":"JMS Carvajal","year":"2021","unstructured":"Carvajal JMS, Rela\u00f1o AG, Torres IR, Martos FJ, Mu\u00f1oz FL, Vera E, Gasc\u00f3n LG, Toset FC, G\u00f3mez IMR, Maldonado A, Carrasco L, Tarradas C, Laguna JG, Luque I (2021) Real-Time PCR validation for Mycobacterium tuberculosis complex detection targeting IS6110 directly from bovine lymph nodes. Front Vet Sci 8:643111. https:\/\/doi.org\/10.3389\/fvets.2021.643111","journal-title":"Front Vet Sci"},{"key":"1779_CR28","doi-asserted-by":"publisher","first-page":"972","DOI":"10.3390\/pathogens12080972","volume":"12","author":"MM Zeineldin","year":"2023","unstructured":"Zeineldin MM, Lehman K, Camp P, Farrel D, Thacher TC (2023) Diagnostic evaluation of the IS1081-Targeted Real-Time PCR for detection of Mycobacterium Bovis DNA in bovine milk samples. Pathogens 12:972. https:\/\/doi.org\/10.3390\/pathogens12080972","journal-title":"Pathogens"},{"key":"1779_CR29","unstructured":"Indea (2014) Portaria conjunta Sedraf-Indea\/MT n. 9 de 14 de novembro de 2014. Institui o regulamento t\u00e9cnico do plano de vigil\u00e2ncia para a Erradica\u00e7\u00e3o da Tuberculose bovina no Estado de Mato Grosso e cria o Programa Estadual de Submiss\u00e3o de Granuloma do Estado de Mato Grosso. https:\/\/www.legisweb.com.br\/legislacao\/?id=276860 (Accessed on January 21st, 2025)"},{"key":"1779_CR30","unstructured":"SAR (2022) Portaria n. 23\/2022 de 10\/5\/2022 da Secretaria de Estado da Agricultura, da Pesca e do Desenvolvimento Rural do estado de Santa Catarina. https:\/\/www.cidasc.sc.gov.br\/inspecao\/files\/2022\/05\/Portaria-SAR-23-2022-1.pdf (Accessed on January 21st, 2025)"},{"key":"1779_CR31","unstructured":"Brasil (2022) Manual de Recomenda\u00e7\u00f5es para o Diagn\u00f3stico Laboratorial de Tuberculose e Micobact\u00e9rias n\u00e3o Tuberculosas de Interesse em Sa\u00fade P\u00fablica no Brasil. Minist\u00e9rio da Sa\u00fade, Bras\u00edlia. https:\/\/www.gov.br\/saude\/pt-br\/centrais-de-conteudo\/publicacoes\/svsa\/tuberculose\/manual-de-recomendacoes-e-para-diagnostico-laboratorial-de-tuberculose-e-micobacterias-nao-tuberculosas-de-interesse-em-saude-publica-no-brasil.pdf (Accessed on September 15th, 2022)"},{"key":"1779_CR32","first-page":"818","volume":"10","author":"RM Warren","year":"2006","unstructured":"Warren RM, van Pittius NCG, Barnard M, Hesseling A, Engelke E, Kock M, Gutierrez MC, Chege GK, Victor TC, Hoal EG, van Helden PD (2006) Differentiation of Mycobacterium tuberculosis complex by PCR amplification of genomic regions of difference. Int J Tuberc Lung Dis 10:818\u2013822. https:\/\/www.ingentaconnect.com\/content\/iuatld\/ijtld\/2006\/00000010\/00000007\/art00019# Accessed on March 15th, 2022)","journal-title":"Int J Tuberc Lung Dis"},{"key":"1779_CR33","unstructured":"Brasil (2015) Manual de Verifica\u00e7\u00e3o de Desempenho de M\u00e9todos para Diagn\u00f3stico Molecular de Doen\u00e7as Infecciosas na Rede Nacional de Laborat\u00f3rios Agropecu\u00e1rios. Minist\u00e9rio da Agricultura, Pecu\u00e1ria e Abastecimento, Bras\u00edlia. https:\/\/www.gov.br\/agricultura\/pt-br\/assuntos\/lfda\/arquivos-publicacoes-laboratorio\/biomol_final_baixa.pdf\/view. (Accessed on March 15th, 2022)"},{"key":"1779_CR34","unstructured":"Sergeant ESG (2018) Epitools Epidemiological Calculators. Ausvet. http:\/\/epitools.ausvet.com.au"},{"key":"1779_CR35","doi-asserted-by":"publisher","first-page":"371","DOI":"10.1016\/j.vetmic.2009.08.029","volume":"140","author":"AL Michel","year":"2010","unstructured":"Michel AL, Muller B, van Helden PD (2010) Mycobacterium Bovis at the animal - human interface: A problem, or not? Vet Microbiol 140:371\u2013378. https:\/\/doi.org\/10.1016\/j.vetmic.2009.08.029","journal-title":"Vet Microbiol"},{"key":"1779_CR36","first-page":"71","volume-title":"Molecular microbiology: diagnostic, principles and practice","author":"CT Wittwer","year":"2004","unstructured":"Wittwer CT, Kusakawa N (2004) Real-time PCR. In: Persing DH, Tenover FC, Versalovic J, Tang JW, Unger ER, Relman DA, White TJ (eds) Molecular microbiology: diagnostic, principles and practice. ASM, Washington, pp 71\u201384"},{"key":"1779_CR37","doi-asserted-by":"publisher","first-page":"45","DOI":"10.2298\/BAH1401045S","volume":"30","author":"A Selim","year":"2014","unstructured":"Selim A, El-haig M, Gaede W (2014) Duplex real-time PCR assay targeting insertion elements IS1081 and IS6110 for detection of Mycobacterium Bovis in lymph nodes of cattle. Inst Anim Husb 30:45\u201349. https:\/\/doi.org\/10.2298\/BAH1401045S","journal-title":"Inst Anim Husb"},{"key":"1779_CR38","doi-asserted-by":"crossref","unstructured":"Araujo CP, Leite CQ, Prince KA, Jorge KSG, Os\u00f3rio ALAR (2005) Mycobacterium Bovis identification by molecular method from post-mortem inspected cattle obtained in abattoirs of Mato Grosso do sul, Brazil. Mem Inst Oswaldo Cruz 100:749\u2013752. https:\/\/memorias.ioc.fiocruz.br\/article\/1797\/mycobacterium-bovis-identification-by-a-molecular-method-from-post-mortem-inspected-cattle-obtained-in-abattoirs-of-mato-grosso-do-sul-brazil (Accessed on April 14th, 2022)","DOI":"10.1590\/S0074-02762005000700013"}],"container-title":["Brazilian Journal of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s42770-025-01779-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s42770-025-01779-7\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s42770-025-01779-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T11:15:00Z","timestamp":1764242100000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s42770-025-01779-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,25]]},"references-count":38,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["1779"],"URL":"https:\/\/doi.org\/10.1007\/s42770-025-01779-7","relation":{},"ISSN":["1517-8382","1678-4405"],"issn-type":[{"type":"print","value":"1517-8382"},{"type":"electronic","value":"1678-4405"}],"subject":[],"published":{"date-parts":[[2025,9,25]]},"assertion":[{"value":"12 May 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 August 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"25 September 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"None of the authors has any financial or personal relationships that could inappropriately influence or bias the content of the paper.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing Interests"}},{"value":"The study was approved by the \u201cEthics committee on the use of animals\u201d of the Faculdade de Medicina Veterin\u00e1ria e Zootecnia of the Universidade de S\u00e3o Paulo (Protocol n. 9802060420).","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical aproval"}}]}}