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This study isolated\n                    <jats:italic>Enterobacteriaceae<\/jats:italic>\n                    and\n                    <jats:italic>Staphylococcaceae<\/jats:italic>\n                    (including\n                    <jats:italic>Mammaliicoccus sciuri<\/jats:italic>\n                    ) from flies collected in bovine and swine slaughterhouses and meat processing industries. Phenotypic AMR was assessed, and the presence of selected antimicrobial resistance and virulence genes (\n                    <jats:italic>mecA<\/jats:italic>\n                    ,\n                    <jats:italic>sea<\/jats:italic>\n                    ,\n                    <jats:italic>see<\/jats:italic>\n                    ,\n                    <jats:italic>sec<\/jats:italic>\n                    ,\n                    <jats:italic>stx1<\/jats:italic>\n                    ,\n                    <jats:italic>stx2<\/jats:italic>\n                    , and\n                    <jats:italic>eae<\/jats:italic>\n                    ) was verified. Out of 60 flies collected, a total of 34 isolates were identified using MALDI-TOF with 13 different bacterial species, including\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    ,\n                    <jats:italic>Citrobacter braakii<\/jats:italic>\n                    ,\n                    <jats:italic>C. freundii<\/jats:italic>\n                    ,\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    ,\n                    <jats:italic>Hafnia alvei<\/jats:italic>\n                    ,\n                    <jats:italic>Enterobacter kobei<\/jats:italic>\n                    ,\n                    <jats:italic>S. aureus<\/jats:italic>\n                    ,\n                    <jats:italic>S. epidermidis<\/jats:italic>\n                    ,\n                    <jats:italic>S. saprophyticus<\/jats:italic>\n                    ,\n                    <jats:italic>S. simulans<\/jats:italic>\n                    ,\n                    <jats:italic>S. warneri<\/jats:italic>\n                    ,\n                    <jats:italic>S. xylosus<\/jats:italic>\n                    , and\n                    <jats:italic>M. sciuri<\/jats:italic>\n                    . Among\n                    <jats:italic>Staphylococcaceae<\/jats:italic>\n                    isolates, 17.6% were resistant to oxacillin and 23.5% to gentamicin. Regarding the\n                    <jats:italic>Enterobacteriaceae<\/jats:italic>\n                    isolates, 41.2% were resistant to streptomycin. Ten isolates (two\n                    <jats:italic>E. coli<\/jats:italic>\n                    , one\n                    <jats:italic>S<\/jats:italic>\n                    .\n                    <jats:italic>epidermidis<\/jats:italic>\n                    , two\n                    <jats:italic>S. saprophyticus<\/jats:italic>\n                    , one\n                    <jats:italic>S. simulans<\/jats:italic>\n                    , one\n                    <jats:italic>S<\/jats:italic>\n                    .\n                    <jats:italic>warneri<\/jats:italic>\n                    and three\n                    <jats:italic>M. sciuri<\/jats:italic>\n                    ) were considered multidrug-resistant (MDR). Notably, none of the targeted genes (\n                    <jats:italic>mecA<\/jats:italic>\n                    ,\n                    <jats:italic>sea<\/jats:italic>\n                    ,\n                    <jats:italic>see<\/jats:italic>\n                    ,\n                    <jats:italic>sec<\/jats:italic>\n                    ,\n                    <jats:italic>stx1<\/jats:italic>\n                    ,\n                    <jats:italic>stx2<\/jats:italic>\n                    ,\n                    <jats:italic>eae<\/jats:italic>\n                    ) were detected by PCR. These findings suggest that flies can disseminate important resistant opportunistic bacteria in meat processing environments, posing concerns due to the capacity of flies to transmit these bacteria across food, processing facilities, animals and humans. These results underscore the need for continuous AMR surveillance and effective vector control to reduce the risks posed by flies in meat processing environments.\n                  <\/jats:p>","DOI":"10.1007\/s42770-026-01883-2","type":"journal-article","created":{"date-parts":[[2026,2,22]],"date-time":"2026-02-22T23:59:13Z","timestamp":1771804753000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Flies from meat processing facilities are carriers of multidrug-resistant Escherichia coli and diverse Staphylococcaceae species"],"prefix":"10.1007","volume":"57","author":[{"given":"Matheus Zorzal Bernardes","family":"Rangel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo Guimar\u00e3es Fernandes","family":"Viana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Julia Camuzzi Ferrari","family":"Storck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina Magri","family":"Ferraz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah Bernardes","family":"Sim\u00f5es","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9ria Modolo","family":"Peterle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Pereira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamella Almeida Freire","family":"Casemiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra Figueiredo","family":"de Castro Nassar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa","family":"Castro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruna Maria","family":"Salotti-Souza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliano Gon\u00e7alves","family":"Pereira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marita Vedovelli","family":"Cardozo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7967-7628","authenticated-orcid":false,"given":"Gabriel Augusto Marques","family":"Rossi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,2,23]]},"reference":[{"key":"1883_CR1","doi-asserted-by":"publisher","first-page":"7641","DOI":"10.2147\/IDR.S428103","volume":"16","author":"AA Abebe","year":"2023","unstructured":"Abebe AA, Birhanu AG (2023) Methicillin-resistant Staphylococcus aureus: molecular mechanisms underlying drug resistance development and novel strategies to combat. 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