{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T05:22:32Z","timestamp":1790140952648,"version":"4.0.1"},"reference-count":24,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T00:00:00Z","timestamp":1783900800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T00:00:00Z","timestamp":1783900800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100018809","name":"Universidade Federal De Pelotas","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100018809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Braz J Microbiol"],"published-print":{"date-parts":[[2026,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Sporothrix brasiliensis<\/jats:italic>\n                    is the predominant etiological agent of feline sporotrichosis in Brazil, where zoonotic dissemination represents an escalating public health concern. Reports of therapeutic refractoriness and the emergence of itraconazole-resistant isolates underscore the pressing need for alternative antifungal strategies. This study evaluated in vitro antifungal activity of a biogenic silver nanoparticle against 24 Brazilian clinical isolates of\n                    <jats:italic>S. brasiliensis<\/jats:italic>\n                    (18 cats and 6 dogs). Broth microdilution assays revealed minimum inhibitory concentrations ranging from 0.47 to 3.75\u00a0\u00b5g\/mL with fungicidal and fungistatic activity. Scanning electron microscopy of treated yeast cells demonstrated pronounced morphological alterations, including cell wall discontinuities, cavitations, surface roughness, and leakage of intracellular contents, confirming severe structural damage. These findings demonstrate that the biogenic silver nanoparticle evaluated exhibits antifungal activity against\n                    <jats:italic>S. brasiliensis<\/jats:italic>\n                    and may represent a promising therapeutic alternative for sporotrichosis. Nonetheless, further in vivo studies are needed to validate their safety and clinical applicability.\n                  <\/jats:p>","DOI":"10.1007\/s42770-026-02022-7","type":"journal-article","created":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T13:16:25Z","timestamp":1783948585000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Biogenic Silver Nanoparticle: In vitro antifungal activity against Sporothrix brasiliensis"],"prefix":"10.1007","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5990-3090","authenticated-orcid":false,"given":"Luci\u00e9le Pereira","family":"de Melo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1481-9829","authenticated-orcid":false,"given":"Caroline Quintana","family":"Braga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5139-4810","authenticated-orcid":false,"given":"Carolina","family":"dos Santos Bermann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-8238-8135","authenticated-orcid":false,"given":"Yasmin Dummer","family":"Ruas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6886-0221","authenticated-orcid":false,"given":"Maria Isabel","family":"de Azevedo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-7625-0775","authenticated-orcid":false,"given":"Gustavo Ruas","family":"de Ara\u00fajo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7266-2319","authenticated-orcid":false,"given":"Renata Os\u00f3rio","family":"de Faria","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5325-044X","authenticated-orcid":false,"given":"Angelita Reis","family":"Gomes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2484-3147","authenticated-orcid":false,"given":"Luciano Aparecido","family":"Panagio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-2608-1383","authenticated-orcid":false,"given":"Karen Almeida","family":"de Ara\u00fajo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4992-3610","authenticated-orcid":false,"given":"Lara Baccarin","family":"Ianiski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8855-9217","authenticated-orcid":false,"given":"S\u00f4nia","family":"de Avila Botton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4374-677X","authenticated-orcid":false,"given":"Daniela Isabel Brayer","family":"Pereira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,7,13]]},"reference":[{"key":"2022_CR1","doi-asserted-by":"publisher","first-page":"894297","DOI":"10.3389\/fcimb.2022.894297","volume":"12","author":"VBS Rabello","year":"2022","unstructured":"Rabello VBS et al (2022) Environmental isolation of Sporothrix brasiliensis in an area with recurrent feline sporotrichosis cases. Front Cell Infect Microbiol 12:894297. https:\/\/doi.org\/10.3389\/fcimb.2022.894297","journal-title":"Front Cell Infect Microbiol"},{"issue":"9","key":"2022_CR2","doi-asserted-by":"publisher","first-page":"921","DOI":"10.3390\/jof9090921","volume":"9","author":"MO Xavier","year":"2023","unstructured":"Xavier MO et al (2023) Sporothrix brasiliensis: epidemiology, therapy, and recent developments. J Fungi (Basel) 9(9):921. https:\/\/doi.org\/10.3390\/jof9090921","journal-title":"J Fungi (Basel)"},{"issue":"4","key":"2022_CR3","doi-asserted-by":"publisher","first-page":"287","DOI":"10.3390\/jof10040287","volume":"10","author":"VR Poester","year":"2024","unstructured":"3.Poester VR et al (2024) Sporothrix brasiliensis causing atypical sporotrichosis in Brazil: a systematic review. J Fungi (Basel) 10(4):287. https:\/\/doi.org\/10.3390\/jof10040287","journal-title":"J Fungi (Basel)"},{"key":"2022_CR4","doi-asserted-by":"publisher","first-page":"857681","DOI":"10.3389\/fnano.2022.857681","volume":"4","author":"JJA Bonilla","year":"2022","unstructured":"Bonilla JJA et al (2022) Silver chitosan nanocomposites are effective to combat sporotrichosis. Front Nanotechnol 4:857681. https:\/\/doi.org\/10.3389\/fnano.2022.857681","journal-title":"Front Nanotechnol"},{"issue":"1","key":"2022_CR5","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1007\/s42770-020-00365-3","volume":"52","author":"IDF Gremi\u00e3o","year":"2021","unstructured":"Gremi\u00e3o IDF et al (2021) Guideline for the management of feline sporotrichosis caused by Sporothrix brasiliensis and literature revision. 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