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Different yeast strains and culture conditions can result in varying lipid yields with distinct fatty acid profiles. Thus, in this study, five strains of\n                    <jats:italic>Y. lipolytica<\/jats:italic>\n                    were tested for microbial oil production using pure glycerol at temperatures of 15\u00a0\u00b0C and 25\u00a0\u00b0C. None of the isolates were considered oleaginous at 15\u00a0\u00b0C. However, at 25\u00a0\u00b0C, the isolates UFMG-CM-Y6114 (from cheese), UFMG-CM-Y4187 (from beach sand), and UFMG-CM-Y6189 (from Antarctica) produced more than 20% lipid content (29.30%, 26.95%, and 34.38%, respectively). No significant differences in lipid content were observed between the UFMG-CM-Y6114 and UFMG-CM-Y4187 strains. The cheese- and Antarctica-derived strains were further tested for their ability to accumulate oil when grown on crude glycerol. Among them, UFMG-CM-Y6189 showed the best performance, producing 9.40\u00a0g\u00b7L\u207b\u00b9 of biomass, 1.90\u00a0g\u00b7L\u207b\u00b9 of lipid yield, and 21.56% lipid content, while consuming 61.3\u00a0g\u00b7L\u207b\u00b9 of glycerol. The fatty acid profile of these lipids was predominantly monounsaturated (59.5%), with oleic acid (49.4%) as the main component. This, combined with a low polyunsaturated fatty acid content (5.2%), makes these lipids particularly suitable for biodiesel production. Therefore, the lipids from the\n                    <jats:italic>Y. lipolytica<\/jats:italic>\n                    strain UFMG-CM-Y6189 represent a promising raw material for biodiesel production from glycerol.\n                  <\/jats:p>","DOI":"10.1007\/s42770-026-02054-z","type":"journal-article","created":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T12:02:02Z","timestamp":1787054522000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Microbial oil production by Yarrowia lipolytica strains using glycerol as carbon source"],"prefix":"10.1007","volume":"57","author":[{"given":"Elisa Duarte Guerra","family":"Coelho","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda","family":"Palladino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisa A. 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