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It is a well-recognized pathogen responsible for bovine mastitis and raw milk contamination; certain strains produce heat-stable enterotoxin capable of causing staphylococcal food poisoning in humans. The emergence of multiple drug-resistant strains of\n                    <jats:italic>S. aureus<\/jats:italic>\n                    has rendered ineffective the use of antibiotics for control purposes. This study determined the antibacterial activity of lytic bacteriophage UHP46 against a single, previously characterized multidrug-resistant mastitis isolate,\n                    <jats:italic>S. aureus<\/jats:italic>\n                    S46, in three dairy matrices \u2014 milk, cheese and yogurt \u2014 under storage conditions representing refrigerated storage (4\u00a0\u00b0C), ambient conditions (25\u00a0\u00b0C) and a 37\u00a0\u00b0C mechanistic reference condition. UHP46 significantly reduced\n                    <jats:italic>S. aureus<\/jats:italic>\n                    S46 counts in milk, achieving reductions of 3.2 and 3.7 log\u2081\u2080 CFU\/mL after 4 and 24\u00a0h at 4\u00a0\u00b0C, 2.1 and 3.1 log\u2081\u2080 CFU\/mL after 4 and 24\u00a0h at 25\u00a0\u00b0C, and 1.8 log\u2081\u2080 CFU\/mL after 3\u00a0h at 37\u00a0\u00b0C. The reduction at 37\u00a0\u00b0C was accompanied by an increase in phage titer from 7.2 to 8.8 log\u2081\u2080 PFU\/mL. Application of UHP46 to cheese resulted in an immediate reduction of 0.6 log\u2081\u2080 CFU\/g after 5\u00a0min of treatment at room temperature. The maximum reduction observed was 2.0 log\u2081\u2080 CFU\/g at 24\u00a0h, with a sustained reduction of 1.9 log\u2081\u2080 CFU\/g maintained through 96\u00a0h of refrigerated storage at 4\u00a0\u00b0C. In yogurt, net reductions on day 6 were 2.8, 2.7, and 1.6 log\u2081\u2080 CFU\/g at 37\u00a0\u00b0C, 25\u00a0\u00b0C, and 4\u00a0\u00b0C, respectively. The findings indicate that UHP46 exhibits significant antibacterial activity against\n                    <jats:italic>S. aureus<\/jats:italic>\n                    S46 within the tested dairy matrices and under the evaluated storage conditions. However, as all challenge experiments utilized a single host strain, its efficacy against a broader panel of\n                    <jats:italic>S. aureus<\/jats:italic>\n                    , including methicillin-resistant\n                    <jats:italic>S. aureus<\/jats:italic>\n                    (MRSA), remains to be determined.\n                  <\/jats:p>","DOI":"10.1007\/s42770-026-02081-w","type":"journal-article","created":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T08:26:02Z","timestamp":1790238362000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Biocontrol of Staphylococcus aureus in dairy products using lytic bacteriophage UHP46: efficacy in milk, cheese, and yogurt"],"prefix":"10.1007","volume":"57","author":[{"given":"Sara","family":"Najeeb","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9802-0429","authenticated-orcid":false,"given":"Imran","family":"Khan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javed","family":"Muhammad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Jahangir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anza","family":"Abbas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aman","family":"Ullah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amjad","family":"Khan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumaira","family":"Miskeen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,24]]},"reference":[{"issue":"2","key":"2081_CR1","doi-asserted-by":"publisher","first-page":"164","DOI":"10.3390\/biologics1020010","volume":"1","author":"AS Abdelsattar","year":"2021","unstructured":"Abdelsattar AS, Safwat A, Nofal R, Elsayed A, Makky S, El-Shibiny A (2021) Isolation and Characterization of Bacteriophage ZCSE6 against Salmonella spp.: Phage Application in Milk. 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