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Relaxation was partially blocked by the addition of the eNOS blocker N\u03c9-nitro-L-arginine, or the large conductance potassium channel blocker paxilline. Combination of N\u03c9-nitro-L-arginine and paxilline prevented mirabegron-mediated relaxation. Imaging revealed that the \u03b23 adrenergic receptors are expressed by both myocyte and microvascular endothelial cells isolated from human myometrium. Nitric oxide production measured by 4-amino-5-methylamino-2',7'-difluorofluorescein diacetate revealed that mirabegron stimulated nitric oxide production in myometrial endothelial cells. These data suggest that both endothelial and smooth muscle cells contribute to relaxation through disparate signaling pathways. Repurposing of approved medications tested in human myometrium as uterine tocolytics can advance prevention of preterm birth. These data argue that further examination of \u03b23 adrenergic receptor signaling in myometrium may reveal mirabegron as a useful tocolytic in combination tocolysis regimens.<\/jats:p>","DOI":"10.1007\/s43032-022-00917-y","type":"journal-article","created":{"date-parts":[[2022,4,5]],"date-time":"2022-04-05T08:02:38Z","timestamp":1649145758000},"page":"124-134","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Title: \u03b23 Adrenergic Receptor Signaling in the Human Myometrium"],"prefix":"10.1007","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7556-575X","authenticated-orcid":false,"given":"Hazik","family":"Asif","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2269-2525","authenticated-orcid":false,"given":"Scott D.","family":"Barnett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2775-6137","authenticated-orcid":false,"given":"Iain L. 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