{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T04:19:08Z","timestamp":1776485948751,"version":"3.51.2"},"reference-count":35,"publisher":"Springer Science and Business Media LLC","issue":"9","license":[{"start":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T00:00:00Z","timestamp":1675728000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T00:00:00Z","timestamp":1675728000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/100000071","name":"National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["K12HD000849"],"award-info":[{"award-number":["K12HD000849"]}],"id":[{"id":"10.13039\/100000071","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000861","name":"Burroughs Wellcome Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000861","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006492","name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI124377"],"award-info":[{"award-number":["AI124377"]}],"id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006492","name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI128751"],"award-info":[{"award-number":["AI128751"]}],"id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006108","name":"National Center for Advancing Translational Sciences","doi-asserted-by":"publisher","award":["UL 1TR002541"],"award-info":[{"award-number":["UL 1TR002541"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Reprod. Sci."],"published-print":{"date-parts":[[2023,9]]},"DOI":"10.1007\/s43032-023-01165-4","type":"journal-article","created":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T18:53:30Z","timestamp":1675796010000},"page":"2655-2664","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Altered Cytokine Production in Human Intervillous Blood T Cells in Preeclampsia"],"prefix":"10.1007","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8539-3960","authenticated-orcid":false,"given":"Ai-ris Y.","family":"Collier","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna M.","family":"Modest","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo A.","family":"Aguayo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esther A.","family":"Bondzie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shivani","family":"Patel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michele R.","family":"Hacker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan H.","family":"Barouch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2023,2,7]]},"reference":[{"issue":"2","key":"1165_CR1","doi-asserted-by":"publisher","first-page":"366","DOI":"10.1097\/aog.0000000000002114","volume":"130","author":"AA Creanga","year":"2017","unstructured":"Creanga AA, Syverson C, Seed K, Callaghan WM. Pregnancy-related mortality in the United States, 2011-2013. Obstet Gynecol. 2017;130(2):366\u201373. https:\/\/doi.org\/10.1097\/aog.0000000000002114.","journal-title":"Obstet Gynecol."},{"issue":"4","key":"1165_CR2","doi-asserted-by":"publisher","first-page":"757","DOI":"10.1161\/HYPERTENSIONAHA.118.11644","volume":"73","author":"C Maric-Bilkan","year":"2019","unstructured":"Maric-Bilkan C, Abrahams VM, Arteaga SS, et al. Research recommendations from the National Institutes of Health Workshop on predicting, preventing, and treating preeclampsia. Hypertens Dallas Tex. 2019;73(4):757\u201366. https:\/\/doi.org\/10.1161\/HYPERTENSIONAHA.118.11644.","journal-title":"Hypertens Dallas Tex"},{"issue":"7","key":"1165_CR3","doi-asserted-by":"publisher","first-page":"1094","DOI":"10.1161\/CIRCRESAHA.118.313276","volume":"124","author":"S Rana","year":"2019","unstructured":"Rana S, Lemoine E, Granger JP, Karumanchi SA. Preeclampsia: pathophysiology, challenges, and perspectives. Circ Res. 2019;124(7):1094\u2013112. https:\/\/doi.org\/10.1161\/CIRCRESAHA.118.313276.","journal-title":"Circ Res."},{"issue":"1","key":"1165_CR4","doi-asserted-by":"publisher","first-page":"108.e1","DOI":"10.1016\/j.ajog.2015.09.066","volume":"214","author":"MY Kim","year":"2016","unstructured":"Kim MY, Buyon JP, Guerra MM, et al. Angiogenic factor imbalance early in pregnancy predicts adverse outcomes in patients with lupus and antiphospholipid antibodies: results of the PROMISSE study. Am J Obstet Gynecol. 2016;214(1):108.e1\u2013108.e14. https:\/\/doi.org\/10.1016\/j.ajog.2015.09.066.","journal-title":"Am J Obstet Gynecol"},{"issue":"1-2","key":"1165_CR5","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1016\/j.jri.2007.08.002","volume":"76","author":"S Saito","year":"2007","unstructured":"Saito S, Sakai M, Sasaki Y, Nakashima A, Shiozaki A. Inadequate tolerance induction may induce pre-eclampsia. J Reprod Immunol. 2007;76(1-2):30\u20139. https:\/\/doi.org\/10.1016\/j.jri.2007.08.002.","journal-title":"J Reprod Immunol."},{"issue":"6","key":"1165_CR6","doi-asserted-by":"publisher","first-page":"899","DOI":"10.1016\/j.immuni.2009.03.019","volume":"30","author":"M Miyara","year":"2009","unstructured":"Miyara M, Yoshioka Y, Kitoh A, et al. Functional delineation and differentiation dynamics of human CD4+ T cells expressing the FoxP3 transcription factor. Immunity. 2009;30(6):899\u2013911. https:\/\/doi.org\/10.1016\/j.immuni.2009.03.019.","journal-title":"Immunity."},{"issue":"5","key":"1165_CR7","doi-asserted-by":"publisher","first-page":"2001","DOI":"10.4049\/jimmunol.1201379","volume":"190","author":"ME Himmel","year":"2013","unstructured":"Himmel ME, MacDonald KG, Garcia RV, Steiner TS, Levings MK. Helios+ and Helios- cells coexist within the natural FOXP3+ T regulatory cell subset in humans. J Immunol Baltim Md. 2013;190(5):2001\u20138. https:\/\/doi.org\/10.4049\/jimmunol.1201379.","journal-title":"J Immunol Baltim Md"},{"issue":"1","key":"1165_CR8","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1111\/j.1365-2567.2004.01869.x","volume":"112","author":"DA Somerset","year":"2004","unstructured":"Somerset DA, Zheng Y, Kilby MD, Sansom DM, Drayson MT. Normal human pregnancy is associated with an elevation in the immune suppressive CD25+ CD4+ regulatory T-cell subset. Immunology. 2004;112(1):38\u201343. https:\/\/doi.org\/10.1111\/j.1365-2567.2004.01869.x.","journal-title":"Immunology."},{"issue":"6","key":"1165_CR9","doi-asserted-by":"publisher","first-page":"534","DOI":"10.1111\/j.1600-0897.2010.00831.x","volume":"63","author":"CWG Redman","year":"2010","unstructured":"Redman CWG, Sargent IL. Immunology of pre-eclampsia. Am J Reprod Immunol N Y N. 2010;63(6):534\u201343. https:\/\/doi.org\/10.1111\/j.1600-0897.2010.00831.x.","journal-title":"Am J Reprod Immunol N Y N"},{"issue":"6","key":"1165_CR10","doi-asserted-by":"publisher","first-page":"1004","DOI":"10.1016\/j.immuni.2018.12.001","volume":"49","author":"MG Roncarolo","year":"2018","unstructured":"Roncarolo MG, Gregori S, Bacchetta R, Battaglia M, Gagliani N. The biology of T regulatory type 1 cells and their therapeutic application in immune-mediated diseases. Immunity. 2018;49(6):1004\u201319. https:\/\/doi.org\/10.1016\/j.immuni.2018.12.001.","journal-title":"Immunity."},{"issue":"6","key":"1165_CR11","doi-asserted-by":"publisher","first-page":"739","DOI":"10.1038\/nm.3179","volume":"19","author":"N Gagliani","year":"2013","unstructured":"Gagliani N, Magnani CF, Huber S, et al. Coexpression of CD49b and LAG-3 identifies human and mouse T regulatory type 1 cells. Nat Med. 2013;19(6):739\u201346. https:\/\/doi.org\/10.1038\/nm.3179.","journal-title":"Nat Med."},{"issue":"9","key":"1165_CR12","doi-asserted-by":"publisher","first-page":"2537","DOI":"10.1016\/j.celrep.2019.04.109","volume":"27","author":"M Salvany-Celades","year":"2019","unstructured":"Salvany-Celades M, van der Zwan A, Benner M, et al. Three types of functional regulatory T cells control T cell responses at the human maternal-fetal interface. Cell Rep. 2019;27(9):2537\u20132547.e5. https:\/\/doi.org\/10.1016\/j.celrep.2019.04.109.","journal-title":"Cell Rep"},{"issue":"2","key":"1165_CR13","doi-asserted-by":"publisher","first-page":"192","DOI":"10.1016\/j.mam.2007.02.006","volume":"28","author":"S Saito","year":"2007","unstructured":"Saito S, Shiozaki A, Nakashima A, Sakai M, Sasaki Y. The role of the immune system in preeclampsia. Mol Aspects Med. 2007;28(2):192\u2013209. https:\/\/doi.org\/10.1016\/j.mam.2007.02.006.","journal-title":"Mol Aspects Med."},{"issue":"1","key":"1165_CR14","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1111\/j.1365-2249.2007.03397.x","volume":"149","author":"Y Sasaki","year":"2007","unstructured":"Sasaki Y, Darmochwal-Kolarz D, Suzuki D, et al. Proportion of peripheral blood and decidual CD4(+) CD25(bright) regulatory T cells in pre-eclampsia. Clin Exp Immunol. 2007;149(1):139\u201345. https:\/\/doi.org\/10.1111\/j.1365-2249.2007.03397.x.","journal-title":"Clin Exp Immunol."},{"issue":"7","key":"1165_CR15","doi-asserted-by":"publisher","first-page":"104648","DOI":"10.1016\/j.isci.2022.104648","volume":"25","author":"J Krop","year":"2022","unstructured":"Krop J, van der Zwan A, Ijsselsteijn ME, et al. Imaging mass cytometry reveals the prominent role of myeloid cells at the maternal-fetal interface. iScience. 2022;25(7):104648. https:\/\/doi.org\/10.1016\/j.isci.2022.104648.","journal-title":"iScience"},{"key":"1165_CR16","doi-asserted-by":"publisher","first-page":"718168","DOI":"10.3389\/fimmu.2021.718168","volume":"12","author":"J Raffetseder","year":"2021","unstructured":"Raffetseder J, Lindau R, van der Veen S, Berg G, Larsson M, Ernerudh J. MAIT cells balance the requirements for immune tolerance and anti-microbial defense during pregnancy. Front Immunol. 2021;12:718168. https:\/\/doi.org\/10.3389\/fimmu.2021.718168.","journal-title":"Front Immunol."},{"key":"1165_CR17","doi-asserted-by":"publisher","unstructured":"Vazquez J, Chavarria M, Li Y, Lopez GE, Stanic AK. Computational flow cytometry analysis reveals a unique immune signature of the human maternal-fetal interface. Am J Reprod Immunol. 2018;79(1). https:\/\/doi.org\/10.1111\/aji.12774.","DOI":"10.1111\/aji.12774"},{"key":"1165_CR18","doi-asserted-by":"publisher","first-page":"e52863","DOI":"10.3791\/52863","volume":"99","author":"Y Xu","year":"2015","unstructured":"Xu Y, Plazyo O, Romero R, Hassan SS, Gomez-Lopez N. Isolation of leukocytes from the human maternal-fetal interface. J Vis Exp. 2015;99:e52863. https:\/\/doi.org\/10.3791\/52863.","journal-title":"J Vis Exp."},{"issue":"11","key":"1165_CR19","doi-asserted-by":"publisher","first-page":"e0188250","DOI":"10.1371\/journal.pone.0188250","volume":"12","author":"TA Nguyen","year":"2017","unstructured":"Nguyen TA, Kahn DA, Loewendorf AI. Maternal-fetal rejection reactions are unconstrained in preeclamptic women. PloS One. 2017;12(11):e0188250. https:\/\/doi.org\/10.1371\/journal.pone.0188250.","journal-title":"PloS One."},{"issue":"5","key":"1165_CR20","doi-asserted-by":"publisher","first-page":"e96723","DOI":"10.1371\/journal.pone.0096723","volume":"9","author":"AI Loewendorf","year":"2014","unstructured":"Loewendorf AI, Nguyen TA, Yesayan MN, Kahn DA. Normal human pregnancy results in maternal immune activation in the periphery and at the uteroplacental interface. PloS One. 2014;9(5):e96723. https:\/\/doi.org\/10.1371\/journal.pone.0096723.","journal-title":"PloS One."},{"issue":"1","key":"1165_CR21","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1097\/AOG.0000000000003018","volume":"133","author":"ACOG Practice Bulletin No","year":"2020","unstructured":"Practice Bulletin No ACOG. 222: Gestational hypertension and preeclampsia. Obstet Gynecol. 2020;133(1):1. https:\/\/doi.org\/10.1097\/AOG.0000000000003018.","journal-title":"Obstet Gynecol."},{"issue":"2","key":"1165_CR22","doi-asserted-by":"publisher","first-page":"377","DOI":"10.1016\/j.jbi.2008.08.010","volume":"42","author":"PA Harris","year":"2009","unstructured":"Harris PA, Taylor R, Thielke R, Payne J, Gonzalez N, Conde JG. Research electronic data capture (REDCap)\u2014a metadata-driven methodology and workflow process for providing translational research informatics support. J Biomed Inform. 2009;42(2):377\u201381. https:\/\/doi.org\/10.1016\/j.jbi.2008.08.010.","journal-title":"J Biomed Inform."},{"issue":"2","key":"1165_CR23","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1016\/j.humimm.2015.12.004","volume":"77","author":"R Bhairavabhotla","year":"2016","unstructured":"Bhairavabhotla R, Kim YC, Glass DD, et al. Transcriptome profiling of human FoxP3+ regulatory T cells. Hum Immunol. 2016;77(2):201\u201313. https:\/\/doi.org\/10.1016\/j.humimm.2015.12.004.","journal-title":"Hum Immunol."},{"issue":"6","key":"1165_CR24","doi-asserted-by":"publisher","first-page":"1130","DOI":"10.1016\/j.cell.2017.05.005","volume":"169","author":"AE Overacre-Delgoffe","year":"2017","unstructured":"Overacre-Delgoffe AE, Chikina M, Dadey RE, et al. Interferon-\u03b3 drives Treg fragility to promote anti-tumor immunity. Cell. 2017;169(6):1130\u20131141.e11. https:\/\/doi.org\/10.1016\/j.cell.2017.05.005.","journal-title":"Cell"},{"issue":"9","key":"1165_CR25","doi-asserted-by":"publisher","first-page":"3834","DOI":"10.3390\/ijerph10093834","volume":"10","author":"W Ai","year":"2013","unstructured":"Ai W, Li H, Song N, Li L, Chen H. Optimal method to stimulate cytokine production and its use in immunotoxicity assessment. Int J Environ Res Public Health. 2013;10(9):3834\u201342. https:\/\/doi.org\/10.3390\/ijerph10093834.","journal-title":"Int J Environ Res Public Health."},{"issue":"6","key":"1165_CR26","doi-asserted-by":"publisher","first-page":"1198","DOI":"10.1016\/j.cell.2016.02.048","volume":"164","author":"X Fan","year":"2016","unstructured":"Fan X, Rudensky AY. Hallmarks of tissue-resident lymphocytes. Cell. 2016;164(6):1198\u2013211. https:\/\/doi.org\/10.1016\/j.cell.2016.02.048.","journal-title":"Cell."},{"issue":"1","key":"1165_CR27","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1146\/annurev-immunol-032712-095954","volume":"31","author":"SN Mueller","year":"2013","unstructured":"Mueller SN, Gebhardt T, Carbone FR, Heath WR. Memory T cell subsets, migration patterns, and tissue residence. Annu Rev Immunol. 2013;31(1):137\u201361. https:\/\/doi.org\/10.1146\/annurev-immunol-032712-095954.","journal-title":"Annu Rev Immunol."},{"key":"1165_CR28","doi-asserted-by":"publisher","first-page":"521","DOI":"10.1146\/annurev-immunol-042617-053214","volume":"37","author":"D Masopust","year":"2019","unstructured":"Masopust D, Soerens AG. Tissue-resident T cells and other resident leukocytes. Annu Rev Immunol. 2019;37:521\u201346. https:\/\/doi.org\/10.1146\/annurev-immunol-042617-053214.","journal-title":"Annu Rev Immunol."},{"issue":"3","key":"1165_CR29","doi-asserted-by":"publisher","first-page":"419","DOI":"10.1042\/CS20171059","volume":"132","author":"SM Scroggins","year":"2018","unstructured":"Scroggins SM, Santillan DA, Lund JM, et al. Elevated vasopressin in pregnant mice induces T-helper subset alterations consistent with human preeclampsia. Clin Sci Lond Engl. 2018;132(3):419\u201336. https:\/\/doi.org\/10.1042\/CS20171059.","journal-title":"Clin Sci Lond Engl"},{"issue":"6","key":"1165_CR30","doi-asserted-by":"publisher","first-page":"413","DOI":"10.1016\/j.placenta.2011.03.010","volume":"32","author":"FP McCarthy","year":"2011","unstructured":"McCarthy FP, Kingdom JC, Kenny LC, Walsh SK. Animal models of preeclampsia; uses and limitations. Placenta. 2011;32(6):413\u20139. https:\/\/doi.org\/10.1016\/j.placenta.2011.03.010.","journal-title":"Placenta."},{"issue":"8","key":"1165_CR31","doi-asserted-by":"publisher","first-page":"584","DOI":"10.1038\/nri1897","volume":"6","author":"A Moffett","year":"2006","unstructured":"Moffett A, Loke C. Immunology of placentation in eutherian mammals. Nat Rev Immunol. 2006;6(8):584\u201394. https:\/\/doi.org\/10.1038\/nri1897.","journal-title":"Nat Rev Immunol."},{"key":"1165_CR32","doi-asserted-by":"publisher","first-page":"1934","DOI":"10.3389\/fimmu.2018.01934","volume":"9","author":"S Tsuda","year":"2018","unstructured":"Tsuda S, Zhang X, Hamana H, et al. Clonally expanded decidual effector regulatory T cells increase in late gestation of normal pregnancy, but not in preeclampsia, in humans. Front Immunol. 2018;9:1934. https:\/\/doi.org\/10.3389\/fimmu.2018.01934.","journal-title":"Front Immunol."},{"key":"1165_CR33","doi-asserted-by":"publisher","first-page":"573","DOI":"10.3389\/fimmu.2019.00573","volume":"10","author":"S Tsuda","year":"2019","unstructured":"Tsuda S, Nakashima A, Shima T, Saito S. New paradigm in the role of regulatory T cells during pregnancy. Front Immunol. 2019;10:573. https:\/\/doi.org\/10.3389\/fimmu.2019.00573.","journal-title":"Front Immunol."},{"issue":"1","key":"1165_CR34","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1161\/HYPERTENSIONAHA.116.07293","volume":"68","author":"K Leavey","year":"2016","unstructured":"Leavey K, Benton SJ, Grynspan D, Kingdom JC, Bainbridge SA, Cox BJ. Unsupervised placental gene expression profiling identifies clinically relevant subclasses of human preeclampsia. Hypertens Dallas Tex. 2016;68(1):137\u201347. https:\/\/doi.org\/10.1161\/HYPERTENSIONAHA.116.07293.","journal-title":"Hypertens Dallas Tex"},{"key":"1165_CR35","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1016\/j.placenta.2019.06.384","volume":"83","author":"K Leavey","year":"2019","unstructured":"Leavey K, Grynspan D, Cox BJ. Both \u201ccanonical\u201d and \u201cimmunological\u201d preeclampsia subtypes demonstrate changes in placental immune cell composition. Placenta. 2019;83:53\u20136. https:\/\/doi.org\/10.1016\/j.placenta.2019.06.384.","journal-title":"Placenta."}],"container-title":["Reproductive Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s43032-023-01165-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s43032-023-01165-4\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s43032-023-01165-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T15:12:49Z","timestamp":1694617969000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s43032-023-01165-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,7]]},"references-count":35,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2023,9]]}},"alternative-id":["1165"],"URL":"https:\/\/doi.org\/10.1007\/s43032-023-01165-4","relation":{},"ISSN":["1933-7191","1933-7205"],"issn-type":[{"value":"1933-7191","type":"print"},{"value":"1933-7205","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,7]]},"assertion":[{"value":"19 September 2022","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"28 December 2022","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 February 2023","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This study was conducted with approval of the BIDMC Institutional Review Board protocol #2016P000360.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics Approval"}},{"value":"All participants provided written informed consent.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to Participate"}},{"value":"Written informed consent included the consent to publish de-identified information resulting from the study and medical record for research purposes.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for Publication"}},{"value":"The authors declare no competing interests.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of Interest"}}]}}