{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T12:24:03Z","timestamp":1758630243143,"version":"3.37.3"},"reference-count":29,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/100007471","name":"Applied Basic Research Foundation of Yunnan Province","doi-asserted-by":"publisher","award":["202001AY070001-228"],"award-info":[{"award-number":["202001AY070001-228"]}],"id":[{"id":"10.13039\/100007471","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Reprod. Sci."],"published-print":{"date-parts":[[2024,4]]},"DOI":"10.1007\/s43032-023-01414-6","type":"journal-article","created":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T17:01:45Z","timestamp":1701450105000},"page":"1139-1145","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Interleukin-6 Concentration in Single-Embryo Medium Is Associated with Blastocyst Formation"],"prefix":"10.1007","volume":"31","author":[{"given":"Hao","family":"Zha","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaolin","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fangjie","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yabin","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Liang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhaoqin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2622-5606","authenticated-orcid":false,"given":"Jie","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2023,12,1]]},"reference":[{"key":"1414_CR1","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1042\/CS20110340","volume":"122","author":"M Mihara","year":"2012","unstructured":"Mihara M, Hashizume M, Yoshida H, Suzuki M, Shiina M. IL-6\/IL-6 receptor system and its role in physiological and pathological conditions. Clin Sci (Lond). 2012;122:143\u201359.","journal-title":"Clin Sci (Lond)"},{"issue":"5","key":"1414_CR2","doi-asserted-by":"publisher","first-page":"115327","DOI":"10.1016\/j.bmc.2020.115327","volume":"28","author":"S Kaur","year":"2020","unstructured":"Kaur S, Bansal Y, Kumar R, Bansal G. A panoramic review of IL-6: structure, pathophysiological roles and inhibitors. Bioorg Med Chem. 2020;28(5):115327.","journal-title":"Bioorg Med Chem"},{"key":"1414_CR3","doi-asserted-by":"publisher","first-page":"234","DOI":"10.1038\/nrclinonc.2018.8","volume":"15","author":"DE Johnson","year":"2018","unstructured":"Johnson DE, O\u2019Keefe RA, Grandis JR. Targeting the IL-6\/JAK\/STAT3 signalling axis in cancer. Nat Rev Clin Oncol. 2018;15:234\u201348.","journal-title":"Nat Rev Clin Oncol"},{"key":"1414_CR4","doi-asserted-by":"publisher","first-page":"820","DOI":"10.1093\/humrep\/dex005","volume":"32","author":"Z Kollmann","year":"2017","unstructured":"Kollmann Z, Schneider S, Fux M, Bersinger NA, von Wolff M. Gonadotrophin stimulation in IVF alters the immune cell profile in follicular fluid and the cytokine concentrations in follicular fluid and serum. Hum Reprod. 2017;32:820\u201331.","journal-title":"Hum Reprod"},{"issue":"2","key":"1414_CR5","doi-asserted-by":"publisher","first-page":"410","DOI":"10.1016\/j.fertnstert.2014.04.032","volume":"102","author":"NE Baskind","year":"2014","unstructured":"Baskind NE, Orsi NM, Sharma V. Follicular-phase ovarian follicular fluid and plasma cytokine profiling of natural cycle in vitro fertilization patients. Fertil Steril. 2014;102(2):410\u20138.","journal-title":"Fertil Steril"},{"key":"1414_CR6","doi-asserted-by":"publisher","DOI":"10.1186\/s13036-018-0134-3","author":"ASK Jones","year":"2019","unstructured":"Jones ASK, Shikanov A. Follicle development as an orchestrated signaling network in a 3D organoid. J Biol Eng. 2019. https:\/\/doi.org\/10.1186\/s13036-018-0134-3.","journal-title":"J Biol Eng"},{"issue":"2","key":"1414_CR7","doi-asserted-by":"publisher","first-page":"527","DOI":"10.1093\/humrep\/del371","volume":"22","author":"R Wu","year":"2007","unstructured":"Wu R, Fujii S, Ryan NK, Van der Hoek KH, Jasper MJ, Sini I, Robertson SA, Robker RL, Norman RJ. Ovarian leukocyte distribution and cytokine\/chemokine mRNA expression in follicular fluid cells in women with polycystic ovary syndrome. Hum Reprod. 2007;22(2):527\u201335.","journal-title":"Hum Reprod"},{"issue":"4","key":"1414_CR8","doi-asserted-by":"publisher","first-page":"284","DOI":"10.1002\/mrd.22285","volume":"81","author":"SL Field","year":"2014","unstructured":"Field SL, Dasgupta T, Cummings M, Orsi NM. Cytokines in ovarian folliculogenesis, oocyte maturation and luteinisation. Mol Reprod. 2014;81(4):284\u2013314.","journal-title":"Mol Reprod"},{"key":"1414_CR9","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1007\/s102380300012","volume":"3","author":"F Kawasaki","year":"2003","unstructured":"Kawasaki F, Kawano Y, Kosay Hasan Z, Narahara H, Miyakawa I. The clinical role of interleukin-6 and interleukin-6 soluble receptor in human follicular fluids. Clin Exp Med. 2003;3:27\u201331.","journal-title":"Clin Exp Med"},{"key":"1414_CR10","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000029624","author":"AZ Stojanovic Gavrilovic","year":"2022","unstructured":"Stojanovic Gavrilovic AZ, Cekovic JM, Parandilovic AZ, Nikolov AB, Sazdanovic PS, Velickovic AM, Andjelkovic MV, Sorak MP. IL-6 of follicular fluid and outcome of in vitro fertilization. Medicine. 2022. https:\/\/doi.org\/10.1097\/MD.0000000000029624.","journal-title":"Medicine"},{"issue":"5","key":"1414_CR11","doi-asserted-by":"publisher","first-page":"1171","DOI":"10.1007\/s10815-020-01737-2","volume":"37","author":"J Yang","year":"2020","unstructured":"Yang J, Yang X, Yang H, Bai Y, Zha H, Jiang F, Meng Y. Interleukin 6 in follicular fluid reduces embryo fragmentation and improves the clinical pregnancy rate. J Assist Reprod Genet. 2020;37(5):1171\u20136.","journal-title":"J Assist Reprod Genet"},{"issue":"23","key":"1414_CR12","doi-asserted-by":"publisher","first-page":"14574","DOI":"10.3390\/ijms232314574","volume":"23","author":"A Viloti\u0107","year":"2022","unstructured":"Viloti\u0107 A, Nacka-Aleksi\u0107 M, Pirkovi\u0107 A, Boji\u0107-Trbojevi\u0107 \u017d, Dekanski D, Jovanovi\u0107 KM. IL-6 and IL-8: an overview of their roles in healthy and pathological pregnancies. Int J Mol Sci. 2022;23(23):14574.","journal-title":"Int J Mol Sci"},{"issue":"5","key":"1414_CR13","doi-asserted-by":"publisher","first-page":"1491","DOI":"10.1095\/biolreprod.102.009639","volume":"68","author":"N Mulayim","year":"2003","unstructured":"Mulayim N, Palter SF, Kayisli UA, Senturk L, Arici A. Chemokine receptor expression in human endometrium. Biol Reprod. 2003;68(5):1491\u20135.","journal-title":"Biol Reprod"},{"key":"1414_CR14","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1016\/j.jare.2020.09.002","volume":"30","author":"M Zhou","year":"2020","unstructured":"Zhou M, Xu H, Zhang D, Si C, Zhou X, Zhao H, Liu Q, Xu B, Zhang A. Decreased PIBF1\/IL6\/p-STAT3 during the mid-secretory phase inhibits human endometrial stromal cell proliferation and decidualization. J Adv Res. 2020;30:15\u201325.","journal-title":"J Adv Res"},{"key":"1414_CR15","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1210\/er.2014-1046","volume":"36","author":"GA Thouas","year":"2015","unstructured":"Thouas GA, Dominguez F, Green MP, Vilella F, Simon C, Gardner DK. Soluble ligands and their receptors in human embryo development and implantation. Endocr Rev. 2015;36:92\u2013130.","journal-title":"Endocr Rev"},{"key":"1414_CR16","doi-asserted-by":"publisher","first-page":"974","DOI":"10.1095\/biolreprod53.4.974","volume":"53","author":"AM Sharkey","year":"1995","unstructured":"Sharkey AM, Dellow K, Blayney M, Macnamee M, Charnock-Jones S, Smith SK. Stage-specific expression of cytokine and receptor messenger ribonucleic acids in human preimplantation embryos. Biol Reprod. 1995;53:974\u201381.","journal-title":"Biol Reprod"},{"key":"1414_CR17","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1111\/j.1600-0897.2012.01142.x","volume":"68","author":"C Yu","year":"2012","unstructured":"Yu C, Wang L, Li J, Guo C, Guo X, Zhang X, et al. Ovarian stimulation reduces IL-6 release from mouse and human pre-implantation embryos. Am J Reprod Immunol. 2012;68:199\u2013204.","journal-title":"Am J Reprod Immunol"},{"key":"1414_CR18","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1093\/humrep\/10.1.171","volume":"10","author":"R Austgulen","year":"1995","unstructured":"Austgulen R, Arntzen KJ, Vatten LJ, Kahn J, Sunde A. Detection of cytokines (interleukin-1, interleukin-6, transforming growth factor-beta) and soluble tumour necrosis factor receptors in embryo culture fluids during in-vitro fertilization. Hum Reprod. 1995;10:171\u20136.","journal-title":"Hum Reprod"},{"key":"1414_CR19","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1111\/j.1600-0897.1997.tb00212.x","volume":"37","author":"RI Bara\u00f1ao","year":"1997","unstructured":"Bara\u00f1ao RI, Piazza A, Rumi LS, Polak de Fried E. Determination of IL-1 and IL-6 levels in human embryo culture-conditioned media. Am J Reprod Immunol. 1997;37:191\u20134.","journal-title":"Am J Reprod Immunol"},{"key":"1414_CR20","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1080\/14647270802302629","volume":"11","author":"R Cutting","year":"2008","unstructured":"Cutting R, Morroll D, Roberts SA, Pickering S, Rutherford A, BFS and ACE. Elective single embryo transfer: guidelines for practice British Fertility Society and Association of Clinical Embryologists. Hum Fertil (Camb). 2008;11:131\u201346.","journal-title":"Hum Fertil (Camb)"},{"key":"1414_CR21","doi-asserted-by":"publisher","first-page":"1155","DOI":"10.1016\/S0015-0282(00)00518-5","volume":"73","author":"DK Gardner","year":"2000","unstructured":"Gardner DK, Lane M, Stevens J, Schlenker T, Schoolcraft WB. Blastocyst score affects implantation and pregnancy outcome: towards a single blastocyst transfer. Fertil Steril. 2000;73:1155\u20138.","journal-title":"Fertil Steril"},{"key":"1414_CR22","doi-asserted-by":"publisher","first-page":"1695","DOI":"10.1007\/s10815-020-01805-7","volume":"37","author":"H Zhong","year":"2020","unstructured":"Zhong H, Sun Q, Chen P, Xiong F, Li G, Wan C, et al. Detection of IL-6, IL-10, and TNF-\u03b1 level in human single-blastocyst conditioned medium using ultrasensitive Single Molecule Array platform and its relationship with embryo quality and implantation: a pilot study. J Assist Reprod Genet. 2020;37:1695\u2013702.","journal-title":"J Assist Reprod Genet"},{"key":"1414_CR23","doi-asserted-by":"publisher","first-page":"908","DOI":"10.1016\/j.fertnstert.2015.06.032","volume":"104","author":"F Dominguez","year":"2015","unstructured":"Dominguez F, Meseguer M, Aparicio-Ruiz B, Piqueras P, Qui\u00f1onero A, Sim\u00f3n C. New strategy for diagnosing embryo implantation potential by combining proteomics and time-lapse technologies. Fertil Steril. 2015;104:908\u201314.","journal-title":"Fertil Steril"},{"key":"1414_CR24","doi-asserted-by":"publisher","first-page":"119","DOI":"10.5653\/cerm.2017.44.3.119","volume":"44","author":"RL Kelley","year":"2017","unstructured":"Kelley RL, Gardner DK. Addition of interleukin-6 to mouse embryo culture increases blastocyst cell number and influences the inner cell mass to trophectoderm ratio. Clin Exp Reprod Med. 2017;44:119.","journal-title":"Clin Exp Reprod Med"},{"key":"1414_CR25","doi-asserted-by":"publisher","DOI":"10.1186\/s12861-019-0182-z","author":"LK Wooldridge","year":"2019","unstructured":"Wooldridge LK, Ealy AD. Interleukin-6 increases inner cell mass numbers in bovine embryos. BMC Dev Biol. 2019. https:\/\/doi.org\/10.1186\/s12861-019-0182-z.","journal-title":"BMC Dev Biol"},{"key":"1414_CR26","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.115.134254","author":"F Meng","year":"2015","unstructured":"Meng F, Forrester-Gauntlett B, Turner P, Henderson H, Oback B. Signal inhibition reveals JAK\/STAT3 pathway as critical for bovine inner cell mass development. Biol Reprod. 2015. https:\/\/doi.org\/10.1095\/biolreprod.115.134254.","journal-title":"Biol Reprod"},{"key":"1414_CR27","doi-asserted-by":"publisher","first-page":"303","DOI":"10.1530\/REP-19-0286","volume":"158","author":"LK Wooldridge","year":"2019","unstructured":"Wooldridge LK, Johnson SE, Cockrum RR, Ealy AD. Interleukin-6 requires JAK to stimulate inner cell mass expansion in bovine embryos. Reproduction. 2019;158:303\u201312.","journal-title":"Reproduction"},{"key":"1414_CR28","doi-asserted-by":"publisher","DOI":"10.1016\/j.jri.2023.104133","author":"PK Javvaji","year":"2023","unstructured":"Javvaji PK, Francis JR, Dhali A, Kolte AP, Mech A, Roy SC, Mishra A. Interleukin-6 stimulates in vitro development of late-stage ovine embryos. J Reprod Immunol. 2023. https:\/\/doi.org\/10.1016\/j.jri.2023.104133.","journal-title":"J Reprod Immunol"},{"issue":"9","key":"1414_CR29","doi-asserted-by":"publisher","first-page":"1991","DOI":"10.1016\/j.stemcr.2022.07.009","volume":"17","author":"M Plana-Carmona","year":"2022","unstructured":"Plana-Carmona M, Stik G, Bulteau R, Segura-Morales C, Alc\u00e1zar N, Wyatt CDR, Klonizakis A, de Andr\u00e9s-Aguayo L, Gasnier M, Tian TV, Torcal Garcia G, Vila-Casades\u00fas M, Plachta N, Serrano M, Francesconi M, Graf T. The trophectoderm acts as a niche for the inner cell mass through C\/EBP\u03b1-regulated IL-6 signaling. Stem Cell Reports. 2022;17(9):1991\u20132004.","journal-title":"Stem Cell Reports"}],"container-title":["Reproductive Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s43032-023-01414-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s43032-023-01414-6\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s43032-023-01414-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,22]],"date-time":"2024-03-22T18:32:13Z","timestamp":1711132333000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s43032-023-01414-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12,1]]},"references-count":29,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2024,4]]}},"alternative-id":["1414"],"URL":"https:\/\/doi.org\/10.1007\/s43032-023-01414-6","relation":{},"ISSN":["1933-7191","1933-7205"],"issn-type":[{"type":"print","value":"1933-7191"},{"type":"electronic","value":"1933-7205"}],"subject":[],"published":{"date-parts":[[2023,12,1]]},"assertion":[{"value":"1 July 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 November 2023","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 December 2023","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Ethical approval was granted by the Institutional Review Board of the Second Affiliated Hospital of Kunming Medical University (Ref. number: \u5ba1-PJ-\u79d1-2022\u201395, date: March 18, 2022).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics Approval"}},{"value":"Written informed consent for participation was not required in accordance with national legislation.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to Participate"}},{"value":"Not applicable.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for Publication"}},{"value":"The authors declare no competing interests.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing Interests"}}]}}