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The association of the key protein expression with clinicopathological features of ICP was further analyzed. A total of 178 DEPs were identified between the ICP and control groups. Functional enrichment analysis showed these proteins were significantly enriched in the PPAR singling pathway by KEGG and PPAR\u03b1\/RXR\u03b1 activation by IPA. Apolipoprotein A2 (APOA2) was the only upregulated protein, which uniquely identified in ICP groups and related to both pathways. Validation of western blotting and immunohistochemical staining analysis showed significantly higher APOA2 expression in the ICP group than in the control group. Furthermore, the expression of APOA2 is associated with clinicopathological features in ICP groups. Receiver operating characteristic (ROC) curve analyses showed that the AUC of APOA2 was 0.8984 (95% confidence interval (CI): 0.772\u20131.000). This study has identified up-regulated APOA2 associated with PPAR singling pathway and PPAR\u03b1\/RXR\u03b1 activation in ICP. Thus, APOA2 may be involved in ICP pathogenesis, serving as a novel biomarker for its diagnosis.<\/jats:p>","DOI":"10.1007\/s43032-023-01437-z","type":"journal-article","created":{"date-parts":[[2024,1,4]],"date-time":"2024-01-04T17:02:02Z","timestamp":1704387722000},"page":"1573-1585","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Proteomic Biomarkers of Intrahepatic Cholestasis of Pregnancy"],"prefix":"10.1007","volume":"31","author":[{"given":"Weijian","family":"Zeng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanyan","family":"Hou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2566-1246","authenticated-orcid":false,"given":"Wei","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,1,4]]},"reference":[{"issue":"11","key":"1437_CR1","doi-asserted-by":"publisher","first-page":"886","DOI":"10.1016\/S2468-1253(21)00306-X","volume":"6","author":"SM Sahoo","year":"2021","unstructured":"Sahoo SM, Mahapatra SJ. 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Each patient has signed an informed consent form.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to Participate"}},{"value":"All data provided in this article does not contain any personal information. All subjects, participants, and researchers gave their consent for publication.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for Publication"}},{"value":"The authors declare no competing interest.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing Interests"}}]}}