{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T04:17:58Z","timestamp":1792642678798,"version":"4.5.0"},"reference-count":33,"publisher":"Springer Science and Business Media LLC","issue":"6","license":[{"start":{"date-parts":[[2024,1,31]],"date-time":"2024-01-31T00:00:00Z","timestamp":1706659200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,1,31]],"date-time":"2024-01-31T00:00:00Z","timestamp":1706659200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Reprod. Sci."],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Thin endometrium (TE), which mainly occurs as a result of severe damage to the endometrial basalis, is one of the prominent etiologies of menstrual abnormalities, infertility, and recurrent miscarriage in women. Previous studies have demonstrated that mesenchymal stem cells (MSCs) are considered ideal cells with multipotency for regenerative medicine and exhibit therapeutic effects on TE through their cellular secretions. However, there is limited research on strategies to enhance MSC secretion to improve their therapeutic efficacy. Herein, we isolated menstrual blood\u2013derived mesenchymal stem cells (MenSCs) from menstruation and transformed them into decidualized stromal cells (DSCs), which are specialized cells with enhanced secretory functions. To assess the therapeutic potential of DSCs compared to MenSCs, we conducted a series of experiments in cells and animals. The results demonstrated that DSCs exhibited changes in morphology compared to MenSCs, with a decrease in cell proliferation but a significant improvement in secretion function. Furthermore, DSCs facilitated the restoration of endometrial thickness and increased the number of glands and blood vessel formation. Most importantly, the pregnancy rates in rats were effectively restored, bringing them closer to normal levels. These findings greatly contribute to our understanding of stem cell therapy for TE and strongly suggest that DSCs could hold significant promise as a potential treatment option for TE.<\/jats:p>","DOI":"10.1007\/s43032-024-01458-2","type":"journal-article","created":{"date-parts":[[2024,1,31]],"date-time":"2024-01-31T11:02:22Z","timestamp":1706698942000},"page":"1662-1673","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["A Novel Method to Repair Thin Endometrium and Restore Fertility Based on Menstruation-Derived Stem Cell"],"prefix":"10.1007","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2125-6887","authenticated-orcid":false,"given":"Kai","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiru","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingxin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianhong","family":"Tong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7759-3447","authenticated-orcid":false,"given":"Shengxia","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,1,31]]},"reference":[{"issue":"1","key":"1458_CR1","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1093\/humupd\/dml045","volume":"13","author":"CE Gargett","year":"2007","unstructured":"Gargett CE. Uterine stem cells: what is the evidence? Hum Reprod Update. 2007;13(1):87\u2013101. https:\/\/doi.org\/10.1093\/humupd\/dml045.","journal-title":"Hum Reprod Update"},{"issue":"5","key":"1458_CR2","doi-asserted-by":"publisher","first-page":"624","DOI":"10.1016\/j.ajog.2020.06.004","volume":"223","author":"HOD Critchley","year":"2020","unstructured":"Critchley HOD, Babayev E, Bulun SE, et al. Menstruation: science and society. Am J Obstet Gynecol. 2020;223(5):624\u201364. https:\/\/doi.org\/10.1016\/j.ajog.2020.06.004.","journal-title":"Am J Obstet Gynecol"},{"issue":"20","key":"1458_CR3","doi-asserted-by":"publisher","first-page":"1346","DOI":"10.1089\/scd.2019.0246","volume":"29","author":"X Ouyang","year":"2020","unstructured":"Ouyang X, You S, Zhang Y, et al. Transplantation of Human amnion epithelial cells improves endometrial regeneration in rat model of intrauterine adhesions. Stem Cells Dev. 2020;29(20):1346\u201362. https:\/\/doi.org\/10.1089\/scd.2019.0246.","journal-title":"Stem Cells Dev"},{"key":"1458_CR4","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1016\/j.bioactmat.2023.03.013","volume":"27","author":"L Feng","year":"2023","unstructured":"Feng L, Wang L, Ma Y, et al. Engineering self-healing adhesive hydrogels with antioxidant properties for intrauterine adhesion prevention. Bioact Mater. 2023;27:82\u201397. https:\/\/doi.org\/10.1016\/j.bioactmat.2023.03.013.","journal-title":"Bioact Mater"},{"key":"1458_CR5","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1016\/j.biopha.2018.03.091","volume":"102","author":"R Azizi","year":"2018","unstructured":"Azizi R, Aghebati-Maleki L, Nouri M, et al. Stem cell therapy in Asherman syndrome and thin endometrium: Stem cell- based therapy. Biomed Pharmacother. 2018;102:333\u201343. https:\/\/doi.org\/10.1016\/j.biopha.2018.03.091.","journal-title":"Biomed Pharmacother"},{"issue":"1","key":"1458_CR6","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1016\/s1472-6483(10)60428-2","volume":"18","author":"B Kumbak","year":"2009","unstructured":"Kumbak B, Erden HF, Tosun S, et al. Outcome of assisted reproduction treatment in patients with endometrial thickness less than 7 mm. Reprod Biomed Online. 2009;18(1):79\u201384. https:\/\/doi.org\/10.1016\/s1472-6483(10)60428-2.","journal-title":"Reprod Biomed Online"},{"issue":"7","key":"1458_CR7","doi-asserted-by":"publisher","first-page":"524","DOI":"10.3109\/09513590.2015.1136616","volume":"32","author":"Z Bu","year":"2016","unstructured":"Bu Z, Wang K, Dai W, et al. Endometrial thickness significantly affects clinical pregnancy and live birth rates in frozen-thawed embryo transfer cycles. 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JAMA. 2022;328(21):2117\u201325. https:\/\/doi.org\/10.1001\/jama.2022.20438.","journal-title":"JAMA"}],"container-title":["Reproductive Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s43032-024-01458-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s43032-024-01458-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s43032-024-01458-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,22]],"date-time":"2024-05-22T17:08:35Z","timestamp":1716397715000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s43032-024-01458-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,31]]},"references-count":33,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,6]]}},"alternative-id":["1458"],"URL":"https:\/\/doi.org\/10.1007\/s43032-024-01458-2","relation":{},"ISSN":["1933-7191","1933-7205"],"issn-type":[{"value":"1933-7191","type":"print"},{"value":"1933-7205","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1,31]]},"assertion":[{"value":"5 September 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 January 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"31 January 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"MenSCs were obtained from the First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, China. The collection of the MenSCs was approved under IRB code NO. 2021KY015, and all the patients signed informed consent before participating in the study. All procedures were conducted according to the principles of the Helsinki Declaration.All animal experiments were conducted by the Guide for the Care and Use of Animals for Research Purposes. All the procedures were approved by the Institutional Animal Care and Use Committee at the First Affiliated Hospital of USTC (code no. 2022-N(A)-119, Title: Regulatory network of Wnt\/\u03b2-catenin signaling pathway in menstrual blood stem cells repairing thin endometrial fertility. Date of approval: 9 May 2023), and followed the guidelines of the International Association for the Study of Pain.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics Approval and Consent to Participate"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing Interests"}},{"value":"All authors gave consent for publication.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for Publication"}}]}}