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Binary logistic regression analysis assessed the relationship between female age, the presence of male factor infertility, and euploidy rates. Blastocyst, utilization, and euploidy rates were significantly higher for cycles using FERTILE PLUS\u2122 compared to DGC (76% vs 56%, <jats:italic>p<\/jats:italic>\u2009=\u20090.002; 60% vs 41%, <jats:italic>p<\/jats:italic>\u2009=\u20090.005, and 40% vs 20%, <jats:italic>p<\/jats:italic>\u2009=\u20090.001, respectively). Although there was an increase in fertilization rates for cycles using FERTILE PLUS\u2122, this was not significant (72% vs 68%, <jats:italic>p<\/jats:italic>\u2009=\u20090.449). The euploidy rates of females\u2009\u2264\u200935 years were significantly increased when the FERTILE PLUS\u2122 sperm preparation method was used, compared to the older age group (OR 2.31, <jats:italic>p<\/jats:italic>\u2009=\u20090.007). No significant association was found between the presence or absence of male factor infertility and euploidy rates between the two cycles. This study provides tentative evidence that the FERTILE PLUS\u2122 microfluidic sorting device for sperm selection can improve blastocyst formation, utilization, and euploidy rates following ICSI in comparison to the DGC method.<\/jats:p>","DOI":"10.1007\/s43032-024-01483-1","type":"journal-article","created":{"date-parts":[[2024,2,23]],"date-time":"2024-02-23T15:02:52Z","timestamp":1708700572000},"page":"1695-1704","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Sperm Preparation with Microfluidic Sperm Sorting Chip May Improve Intracytoplasmic Sperm Injection Outcomes Compared to Density Gradient Centrifugation"],"prefix":"10.1007","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9538-4606","authenticated-orcid":false,"given":"Maria","family":"Banti","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estee","family":"Van Zyl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimitrios","family":"Kafetzis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,2,23]]},"reference":[{"key":"1483_CR1","unstructured":"World Health Organization. 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