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To do this, pregnant human myometrial strips (term, not-in-labor) were treated with cumulative concentrations of the contraction-blocking agents, nifedipine (NIF), indomethacin (IND), 2-aminoethoxydiphenyl borate (2-APB), glycyl-h-1152 (GH), aminophylline (AMP), or rolipram (ROL)). Concentration-response curves were generated using either AUC or amplitude alone as the index of contraction inhibition, from which tocolytic IC<jats:sub>25<\/jats:sub> and IC<jats:sub>50<\/jats:sub> concentrations were calculated and compared (i.e. IC<jats:sub>25(AUC)<\/jats:sub> vs. IC<jats:sub>25(Amplitude)<\/jats:sub>). The effects of each tocolytic on contraction frequency were also quantified. To assess accuracy, each tocolytic was applied to contracting strips as a single treatment at their respective IC<jats:sub>25(AUC\/Amplitude)<\/jats:sub> and IC<jats:sub>50(AUC\/Amplitude)<\/jats:sub>, and then the inhibitory effect re-quantified against both contraction AUC and amplitude alone. Significant differences between IC<jats:sub>50(AUC)<\/jats:sub> and IC<jats:sub>50(Amplitude)<\/jats:sub> were detected for AMP (318.5 vs. 450 \u00b5M), ROL (4.3 vs. 55 \u00b5M), and IND (59.5 vs. 75 \u00b5M), whereas AUC versus amplitude-based IC<jats:sub>50<\/jats:sub> concentrations were comparable for NIF (10 vs. 10 nM), GH (18.2 vs. 15 \u00b5M), and 2-APB (53 vs. 57 \u00b5M). Similarly, the determined IC<jats:sub>25(AUC)<\/jats:sub> and IC<jats:sub>25(Amplitude)<\/jats:sub> were again significantly different for AMP (175 vs. 277 \u00b5M), ROL (515 nM vs. 15 \u00b5M), and IND (28 vs. 42 \u00b5M), but also for 2-APB (27 vs. 40 \u00b5M). The confirmation studies revealed that a single dose of the tocolytics at their determined IC<jats:sub>25(AUC)<\/jats:sub> and IC<jats:sub>50(AUC)<\/jats:sub> concentrations consistently reduced contraction AUC by approximately 25% and 50%, respectively, whereas single doses of the IC<jats:sub>25(Amplitude)<\/jats:sub> and IC<jats:sub>50(Amplitude)<\/jats:sub> concentrations revealed inconsistent results. Of the six tocolytics, four reduced contraction amplitude by &gt;\u200925% when applied as a single dose at IC<jats:sub>25(Amplitude)<\/jats:sub>, while three reduced contraction amplitude by &gt;\u200950% when applied at IC<jats:sub>50(Amplitude)<\/jats:sub>. Our experimental data indicate that when determining tocolytic potency, assessment of ex vivo contraction traces via AUC is more accurate and consistent than assessment via amplitude alone. Our study finds that whilst tocolytics that increase contraction frequency (while decreasing amplitude) can be assessed by either AUC or amplitude-based determination, tocolytics that either significantly reduce or largely do not affect contraction frequency should only be analyzed via AUC.<\/jats:p>","DOI":"10.1007\/s43032-025-01864-0","type":"journal-article","created":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T16:07:54Z","timestamp":1745510874000},"page":"2027-2049","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Assessing Tocolytic Potency: Variability and Accuracy of AUC Versus Amplitude-Based Assessment of Pregnant Human Myometrial Contractions Ex Vivo"],"prefix":"10.1007","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6999-6948","authenticated-orcid":false,"given":"Md Reduanul","family":"Hossain","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8647-9213","authenticated-orcid":false,"given":"Marina","family":"Paul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4009-5628","authenticated-orcid":false,"given":"Jorge M.","family":"Tolosa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4720-0280","authenticated-orcid":false,"given":"Roger","family":"Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3064-6358","authenticated-orcid":false,"given":"Jonathan W.","family":"Paul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,4,24]]},"reference":[{"key":"1864_CR1","doi-asserted-by":"publisher","first-page":"e137","DOI":"10.1016\/S2214-109X(18)30451-0","volume":"7","author":"S Chawanpaiboon","year":"2019","unstructured":"Chawanpaiboon S, Vogel JP, Moller A-B, Lumbiganon P, Petzold M, Hogan D, Landoulsi S, Jampathong N, Kongwattanakul K, Laopaiboon M, Lewis C, Rattanakanokchai S, Teng DN, Thinkhamrop J, Watananirun K, Zhang J, Zhou W, G\u00fclmezoglu AM. 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