{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T01:13:13Z","timestamp":1761181993250,"version":"build-2065373602"},"reference-count":41,"publisher":"Springer Science and Business Media LLC","issue":"10","license":[{"start":{"date-parts":[[2025,8,25]],"date-time":"2025-08-25T00:00:00Z","timestamp":1756080000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,8,25]],"date-time":"2025-08-25T00:00:00Z","timestamp":1756080000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Reprod. Sci."],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Abnormal development of granulosa cells is widely recognized as a critical factor contributing to polycystic ovary syndrome (PCOS). However, the precise etiology and underlying mechanisms of this disorder remain largely elusive. Accumulating evidence suggests that dysregulation of microRNAs (miRNAs) plays a pivotal role in the pathogenesis of PCOS. In this study, we systematically investigated the functional impact of miR-30c-5p on the human cumulus cells (CCs). Our findings revealed that miR-30c-5p suppresses the proliferation and induces apoptosis in the human granulosa-like tumor cell line (KGN) via targeting SIRT1. Notably, the expression level of miR-30c-5p was significantly elevated in PCOS patients compared to healthy controls, whereas the expression of SIRT1 was markedly reduced. A negative correlation was observed between miR-30c-5p and SIRT1 expression. Mechanistically, upregulation of miR-30c-5p led to decreased expression of SIRT1 and Bcl-2 proteins, while simultaneously enhancing the expression of Bax proteins. Furthermore, our data confirmed that SIRT1 serves as a direct target of miR-30c-5p. Collectively, these results indicate that miR-30c-5p promotes apoptosis of GCs by directly targeting SIRT1, thereby representing a novel molecular target for improving GC dysfunction in PCOS patients.<\/jats:p>","DOI":"10.1007\/s43032-025-01932-5","type":"journal-article","created":{"date-parts":[[2025,8,25]],"date-time":"2025-08-25T16:25:25Z","timestamp":1756139125000},"page":"3400-3409","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Mechanistic Study of MiR-30c-5p Regulation of SIRT Expression in Polycystic Ovary Syndrome"],"prefix":"10.1007","volume":"32","author":[{"given":"Lifei","family":"Zhou","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Luo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pingping","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fangfang","family":"Dai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingming","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shusong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9494-8462","authenticated-orcid":false,"given":"Yali","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,8,25]]},"reference":[{"issue":"3","key":"1932_CR1","doi-asserted-by":"publisher","first-page":"833","DOI":"10.1007\/s43032-019-00086-5","volume":"27","author":"MH Hu","year":"2020","unstructured":"Hu MH, Zheng SX, Yin H, Zhu XY, Lu FT, Tong XH, Liu YS, Zhang YW, Xu B. Identification of microRNAs that regulate the mapk pathway in human cumulus cells from PCOS women with insulin resistance. Reprod Sci. 2020;27(3):833\u201344.","journal-title":"Reprod Sci"},{"issue":"1","key":"1932_CR2","doi-asserted-by":"publisher","first-page":"68","DOI":"10.1186\/s12958-019-0505-8","volume":"17","author":"T He","year":"2019","unstructured":"He T, Sun Y, Zhang Y, Zhao S, Zheng Y, Hao G, Shi Y. MicroRNA-200b and microRNA-200c are up-regulated in PCOS granulosa cell and inhibit KGN cell proliferation via targeting PTEN. Reprod Biol Endocrinol. 2019;17(1):68.","journal-title":"Reprod Biol Endocrinol"},{"issue":"2","key":"1932_CR3","doi-asserted-by":"publisher","first-page":"501","DOI":"10.1007\/s00404-022-06461-z","volume":"306","author":"Y Li","year":"2022","unstructured":"Li Y, Wu X, Miao S, Cao Q. MiR-383-5p promotes apoptosis of ovarian granulosa cells by targeting CIRP through the PI3K\/AKT signaling pathway. Arch Gynecol Obstet. 2022;306(2):501\u201312.","journal-title":"Arch Gynecol Obstet"},{"key":"1932_CR4","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.779718","volume":"9","author":"Y Gao","year":"2021","unstructured":"Gao Y, Chen J, Ji R, Ding J, Zhang Y, Yang J. USP25 Regulates the proliferation and apoptosis of ovarian granulosa cells in polycystic ovary syndrome by modulating the PI3K\/AKT pathway via deubiquitinating PTEN. Front Cell Dev Biol. 2021;9: 779718.","journal-title":"Front Cell Dev Biol"},{"issue":"1\u20132","key":"1932_CR5","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1007\/s11010-019-03678-6","volume":"465","author":"Y Ding","year":"2020","unstructured":"Ding Y, He P, Li Z. MicroRNA-9119 regulates cell viability of granulosa cells in polycystic ovarian syndrome via mediating dicer expression. Mol Cell Biochem. 2020;465(1\u20132):187\u201397.","journal-title":"Mol Cell Biochem"},{"key":"1932_CR6","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1016\/j.gene.2019.04.082","volume":"706","author":"B Chen","year":"2019","unstructured":"Chen B, Xu P, Wang J, Zhang C. The role of MiRNA in polycystic ovary syndrome (PCOS). Gene. 2019;706:91\u20136.","journal-title":"Gene"},{"issue":"10","key":"1932_CR7","doi-asserted-by":"publisher","first-page":"4429","DOI":"10.18632\/aging.204745","volume":"15","author":"LH Zhou","year":"2023","unstructured":"Zhou LH, Zou H, Hao JY, et al. Metformin inhibits ovarian granular cell pyroptosis through the miR-670-3p\/NOX2\/ROS pathway. Aging (Albany NY). 2023;15(10):4429\u201343.","journal-title":"Aging (Albany NY)"},{"issue":"7","key":"1932_CR8","doi-asserted-by":"publisher","first-page":"629","DOI":"10.1080\/09513590.2021.1928066","volume":"37","author":"SL Peng","year":"2021","unstructured":"Peng SL, Wu QF, Xie Q, et al. PATL2 regulated the apoptosis of ovarian granulosa cells in patients with PCOS. Gynecol Endocrinol. 2021;37(7):629\u201334.","journal-title":"Gynecol Endocrinol"},{"issue":"11","key":"1932_CR9","doi-asserted-by":"publisher","first-page":"4119","DOI":"10.1007\/s12011-020-02524-x","volume":"199","author":"Q Chen","year":"2021","unstructured":"Chen Q, Li Z, Xu Z, et al. miR-378d is involved in the regulation of apoptosis and autophagy of and E2 secretion from cultured ovarian granular cells treated by sodium fluoride. Biol Trace Elem Res. 2021;199(11):4119\u201328.","journal-title":"Biol Trace Elem Res"},{"issue":"2","key":"1932_CR10","doi-asserted-by":"publisher","first-page":"4361","DOI":"10.1080\/21655979.2022.2033014","volume":"13","author":"H Chen","year":"2022","unstructured":"Chen H, Fu Y, Guo Z, et al. MicroRNA-29c-3p participates in insulin function to modulate polycystic ovary syndrome via targeting Forkhead box O 3. Bioengineered. 2022;13(2):4361\u201371.","journal-title":"Bioengineered"},{"issue":"1","key":"1932_CR11","doi-asserted-by":"publisher","first-page":"297","DOI":"10.1210\/en.2017-00219","volume":"159","author":"M Wang","year":"2018","unstructured":"Wang M, Sun J, Xu B, Chrusciel M, Gao J, Bazert M, Stelmaszewska J, Xu Y, Zhang H, Pawelczyk L, Sun F, Tsang SY, Rahman N, Wolczynski S, Li X. Functional characterization of MicroRNA-27a-3p expression in human polycystic ovary syndrome. Endocrinology. 2018;159(1):297\u2013309.","journal-title":"Endocrinology"},{"issue":"7","key":"1932_CR12","doi-asserted-by":"publisher","first-page":"983","DOI":"10.3390\/cells12070983","volume":"12","author":"PB Udesen","year":"2023","unstructured":"Udesen PB, S\u00f8rensen AE, Svendsen R, Frisk NLS, Hess AL, Aziz M, Wissing MLM, Englund ALM, Dalgaard LT. Circulating miRNAs in women with polycystic ovary syndrome: a longitudinal cohort study. Cells. 2023;12(7):983.","journal-title":"Cells"},{"issue":"4","key":"1932_CR13","doi-asserted-by":"publisher","first-page":"5055","DOI":"10.1002\/cam4.5246","volume":"12","author":"Q Wu","year":"2023","unstructured":"Wu Q, Li G, Gong L, Cai J, Chen L, Xu X, Liu X, Zhao J, Zeng Y, Gao R, Yu L, Wang Z. Identification of miR-30c-5p as a tumor suppressor by targeting the m6 a reader HNRNPA2B1 in ovarian cancer. Cancer Med. 2023;12(4):5055\u201370.","journal-title":"Cancer Med"},{"issue":"1","key":"1932_CR14","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1016\/j.ygyno.2019.04.005","volume":"154","author":"X Xu","year":"2019","unstructured":"Xu X, Kong X, Liu T, Zhou L, Wu J, Fu J, Wang Y, Zhu M, Yao S, Ding Y, Ding L, Li R, Zhu X, Tang X, Zhang Y, Yang Q, Ling J, Zhou H. Metastasis-associated protein 1, modulated by miR-30c, promotes endometrial cancer progression through AKT\/mTOR\/4E-BP1 pathway. Gynecol Oncol. 2019;154(1):207\u201317.","journal-title":"Gynecol Oncol"},{"issue":"1","key":"1932_CR15","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1186\/s12967-021-03226-1","volume":"20","author":"T Zheng","year":"2022","unstructured":"Zheng T, Zhou Y, Xu X, Qi X, Liu J, Pu Y, Zhang S, Gao X, Luo X, Li M, Wang X, Dong L, Wang Y, Mao C. MiR-30c-5p loss-induced PELI1 accumulation regulates cell proliferation and migration via activating PI3K\/AKT pathway in papillary thyroid carcinoma. J Transl Med. 2022;20(1):20.","journal-title":"J Transl Med"},{"issue":"8","key":"1932_CR16","doi-asserted-by":"publisher","first-page":"754","DOI":"10.1089\/dna.2019.4622","volume":"38","author":"Z Chen","year":"2019","unstructured":"Chen Z, Ou H, Wu H, Wu P, Mo Z. Role of microRNA in the pathogenesis of polycystic ovary syndrome. DNA Cell Biol. 2019;38(8):754\u201362.","journal-title":"DNA Cell Biol"},{"issue":"8","key":"1932_CR17","doi-asserted-by":"publisher","first-page":"1816","DOI":"10.3390\/biomedicines10081816","volume":"10","author":"SG Vitale","year":"2022","unstructured":"Vitale SG, Fulghesu AM, Miku\u0161 M, Watrowski R, D\u2019Alterio MN, Lin LT, Shah M, Reyes-Mu\u00f1oz E, Sathyapalan T, Angioni S. The translational role of miRNA in polycystic ovary syndrome: from bench to bedside-a systematic literature review. Biomedicines. 2022;10(8):1816.","journal-title":"Biomedicines"},{"issue":"5","key":"1932_CR18","doi-asserted-by":"publisher","first-page":"1304","DOI":"10.1159\/000358698","volume":"33","author":"W Long","year":"2014","unstructured":"Long W, Zhao C, Ji C, Ding H, Cui Y, Guo X, Shen R, Liu J. Characterization of serum microRNAs profile of PCOS and identification of novel non-invasive biomarkers. Cell Physiol Biochem. 2014;33(5):1304\u201315.","journal-title":"Cell Physiol Biochem"},{"issue":"3","key":"1932_CR19","doi-asserted-by":"publisher","first-page":"267","DOI":"10.1093\/humupd\/dmy003","volume":"24","author":"C Tatone","year":"2018","unstructured":"Tatone C, Di Emidio G, Barbonetti A, Carta G, Luciano AM, Falone S, Amicarelli F. Sirtuins in gamete biology and reproductive physiology: emerging roles and therapeutic potential in female and male infertility. Hum Reprod Update. 2018;24(3):267\u201389.","journal-title":"Hum Reprod Update"},{"issue":"1","key":"1932_CR20","doi-asserted-by":"publisher","first-page":"209","DOI":"10.3390\/cells9010209","volume":"9","author":"GD Emidio","year":"2020","unstructured":"Emidio GD, Placidi M, Rea F, Rossi G, Falone S, Cristiano L, Nottola S, D\u2019Alessandro AM, Amicarelli F, Palmerini MG, Tatone C. Methylglyoxal-dependent glycative stress and deregulation of SIRT1 functional network in the ovary of pcos mice. Cells. 2020;9(1):209.","journal-title":"Cells"},{"key":"1932_CR21","doi-asserted-by":"publisher","first-page":"250","DOI":"10.1016\/j.gene.2018.11.025","volume":"686","author":"H Luo","year":"2019","unstructured":"Luo H, Han Y, Liu J, Zhang Y. Identification of microRNAs in granulosa cells from patients with different levels of ovarian reserve function and the potential regulatory function of miR-23a in granulosa cell apoptosis. Gene. 2019;686:250\u201360.","journal-title":"Gene"},{"key":"1932_CR22","doi-asserted-by":"crossref","unstructured":"Revised 2003 consensus on diagnostic criteria and long-term health risks related to polycystic ovary syndrome (PCOS). Hum Reprod. 2004;19(1):41\u20137.","DOI":"10.1093\/humrep\/deh098"},{"issue":"9","key":"1932_CR23","doi-asserted-by":"publisher","first-page":"2625","DOI":"10.1007\/s43032-022-00930-1","volume":"29","author":"Y Hong","year":"2022","unstructured":"Hong Y, Wu J, Yu S, Hui M, Lin S. Serum-derived exosomal microRNAs in lipid metabolism in polycystic ovary syndrome. Reprod Sci. 2022;29(9):2625\u201335.","journal-title":"Reprod Sci"},{"key":"1932_CR24","doi-asserted-by":"crossref","unstructured":"Liu, Y, Zhang, S, Chen, L, et al. The molecular mechanism of miR-96\u20135p in the pathogenesis and treatment of polycystic ovary syndrome. Transl Res. 2022;256:1\u201313.","DOI":"10.1016\/j.trsl.2022.12.007"},{"issue":"7","key":"1932_CR25","first-page":"3660","volume":"12","author":"J Du","year":"2020","unstructured":"Du J, Lin X, Wu R, et al. miR-424 suppresses proliferation and promotes apoptosis of human ovarian granulosa cells by targeting Apelin and APJ expression. Am J Transl Res. 2020;12(7):3660\u201373.","journal-title":"Am J Transl Res"},{"issue":"4","key":"1932_CR26","doi-asserted-by":"publisher","first-page":"359","DOI":"10.3892\/etm.2021.9790","volume":"21","author":"Y Wei","year":"2021","unstructured":"Wei Y, Wang Z, Wei L, et al. MicroRNA-874-3p promotes testosterone-induced granulosa cell apoptosis by suppressing HDAC1-mediated p53 deacetylation. Exp Ther Med. 2021;21(4):359.","journal-title":"Exp Ther Med"},{"issue":"1","key":"1932_CR27","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1186\/s12943-021-01372-0","volume":"20","author":"Y Guo","year":"2021","unstructured":"Guo Y, Guo Y, Chen C, et al. Circ3823 contributes to growth, metastasis and angiogenesis of colorectal cancer: involvement of miR-30c-5p\/TCF7 axis. Mol Cancer. 2021;20(1):93.","journal-title":"Mol Cancer"},{"issue":"17","key":"1932_CR28","first-page":"7628","volume":"23","author":"WN Hu","year":"2019","unstructured":"Hu WN, Duan ZY, Wang Q, et al. The suppression of ox-LDL-induced inflammatory response and apoptosis of HUVEC by lncRNA XIAT knockdown via regulating miR-30c-5p\/PTEN axis. Eur Rev Med Pharmaco. 2019;23(17):7628\u201338.","journal-title":"Eur Rev Med Pharmaco"},{"issue":"3","key":"1932_CR29","doi-asserted-by":"publisher","first-page":"6359","DOI":"10.1080\/21655979.2022.2031419","volume":"13","author":"T Liu","year":"2022","unstructured":"Liu T, Jiang F, Yu LY, et al. Lidocaine represses proliferation and cisplatin resistance in cutaneous squamous cell carcinoma via miR-30c\/SIRT1 regulation. Bioengineered. 2022;13(3):6359\u201370.","journal-title":"Bioengineered"},{"issue":"1","key":"1932_CR30","doi-asserted-by":"publisher","first-page":"337","DOI":"10.21037\/tcr-19-2957","volume":"10","author":"LQ Yuan","year":"2021","unstructured":"Yuan LQ, Zhang T, Xu L, et al. miR-30c-5p inhibits glioma proliferation and invasion via targeting Bcl2. Transl Cancer Res. 2021;10(1):337\u201348.","journal-title":"Transl Cancer Res"},{"key":"1932_CR31","doi-asserted-by":"crossref","unstructured":"He, Z, Tian, M, Fu, X. Reduced expression of miR-30c-5p promotes hepatocellular carcinoma progression by targeting RAB32. Mol Ther Nucleic Acids. 2021;26:603\u2013612.","DOI":"10.1016\/j.omtn.2021.08.033"},{"issue":"1","key":"1932_CR32","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1186\/s12711-020-00592-0","volume":"52","author":"M G\u00f2dia","year":"2020","unstructured":"G\u00f2dia M, Reverter A, Gonz\u00e1lez-Prendes R, Ramayo-Caldas Y, Castell\u00f3 A, Rodr\u00edguez-Gil JE, S\u00e1nchez A, Clop A. A systems biology framework integrating GWAS and RNA-seq to shed light on the molecular basis of sperm quality in swine. Genet Sel Evol. 2020;52(1):72.","journal-title":"Genet Sel Evol"},{"issue":"4","key":"1932_CR33","doi-asserted-by":"publisher","first-page":"3509","DOI":"10.1021\/acsnano.3c10884","volume":"18","author":"H Liu","year":"2024","unstructured":"Liu H, Zhang X, Zhang M, Zhang S, Li J, Zhang Y, Wang Q, Cai JP, Cheng K, Wang S. Mesenchymal stem cell derived exosomes repair uterine injury by targeting transforming growth factor-\u03b2 signaling. ACS Nano. 2024;18(4):3509\u201319.","journal-title":"ACS Nano"},{"issue":"3","key":"1932_CR34","doi-asserted-by":"publisher","first-page":"1853","DOI":"10.3390\/ijms25031853","volume":"25","author":"L Chico-Sordo","year":"2024","unstructured":"Chico-Sordo L, Ruiz-Mart\u00ednez T, Toribio M, Gonz\u00e1lez-Mart\u00edn R, Spagnolo E, Dom\u00ednguez F, Hern\u00e1ndez A, Garc\u00eda-Velasco JA. Identification of miR-30c-5p microRNA in serum as a candidate biomarker to diagnose endometriosis. Int J Mol Sci. 2024;25(3):1853.","journal-title":"Int J Mol Sci"},{"issue":"10","key":"1932_CR35","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0275031","volume":"17","author":"G De Nardo Maffazioli","year":"2022","unstructured":"De Nardo Maffazioli G, Baracat EC, Soares JM, et al. Evaluation of circulating microRNA profiles in brazilian women with polycystic ovary syndrome: a preliminary study. PLoS ONE. 2022;17(10): e0275031.","journal-title":"PLoS ONE"},{"key":"1932_CR36","doi-asserted-by":"crossref","unstructured":"Hu, J, Lin, F, Yin, Y, et al. Adipocyte-derived exosomal miR-30c-5p promotes ovarian angiogenesis in polycystic ovary syndrome via the SOCS3\/STAT3\/VEGFA pathway. J Steroid Biochem. 2023;230:106278.","DOI":"10.1016\/j.jsbmb.2023.106278"},{"issue":"3","key":"1932_CR37","doi-asserted-by":"publisher","first-page":"2073","DOI":"10.3390\/cimb45030133","volume":"45","author":"X Li","year":"2023","unstructured":"Li X, He Y, Wu S, et al. Regulation of SIRT1 in ovarian function: PCOS treatment. Curr Issues Mol Biol. 2023;45(3):2073\u201389.","journal-title":"Curr Issues Mol Biol"},{"key":"1932_CR38","doi-asserted-by":"publisher","first-page":"1260","DOI":"10.1007\/s10495-017-1386-y","volume":"22","author":"Y Han","year":"2017","unstructured":"Han Y, Luo H, Wang H, Cai J, Zhang Y. SIRT1 induces resistance to apoptosis in human granulosa cells by activating the ERK pathway and inhibiting NF-\u03baB signaling with anti-inflammatory functions. Apoptosis. 2017;22:1260\u201372.","journal-title":"Apoptosis"},{"key":"1932_CR39","doi-asserted-by":"crossref","unstructured":"Wu, YX, Yang, XY, Han, BS, et al. Naringenin regulates gut microbiota and SIRT1\/ PGC-1\u0251 signaling pathway in rats with letrozole-induced polycystic ovary syndrome. Biomed Pharmacother. 2022;153:113286.","DOI":"10.1016\/j.biopha.2022.113286"},{"issue":"2","key":"1932_CR40","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1080\/19396368.2022.2125855","volume":"69","author":"P Huo","year":"2022","unstructured":"Huo P, Li M, Le J, et al. Resveratrol improves follicular development of PCOS rats via regulating glycolysis pathway and targeting SIRT1. Syst Biol Reprod Med. 2022;69(2):153\u201365.","journal-title":"Syst Biol Reprod Med"},{"key":"1932_CR41","doi-asserted-by":"crossref","unstructured":"Gonz\u00e1lez-Fern\u00e1ndez, R, Mart\u00edn-Ram\u00edrez, R, Rotoli, D, et al. Granulosa-lutein cell sirtuin gene expression profiles differ between normal donors and infertile women. Int J Mol Sci. 2019;21(1).","DOI":"10.3390\/ijms21010295"}],"container-title":["Reproductive Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s43032-025-01932-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s43032-025-01932-5\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s43032-025-01932-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T17:41:03Z","timestamp":1761154863000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s43032-025-01932-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,25]]},"references-count":41,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2025,10]]}},"alternative-id":["1932"],"URL":"https:\/\/doi.org\/10.1007\/s43032-025-01932-5","relation":{},"ISSN":["1933-7191","1933-7205"],"issn-type":[{"type":"print","value":"1933-7191"},{"type":"electronic","value":"1933-7205"}],"subject":[],"published":{"date-parts":[[2025,8,25]]},"assertion":[{"value":"25 April 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 July 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"25 August 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All experiments and the ovarian granulosa cells used were approved by the Ethics Committee of Hebei Xingtai Fertility Hospital 2024 (Approval No. 202401).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical Approval"}},{"value":"Follicular fluid and clinical data were collected with the informed consent of patients.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Informed Consent"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing Interests"}}]}}