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Its level is intricately linked to the homeostasis of the biological environment, underscoring the significance of developing techniques capable of detecting H<jats:sub>2<\/jats:sub>S in biological systems. A single probe that offers versatility across different detection techniques opens opportunities for advancements in sensing H<jats:sub>2<\/jats:sub>S in various fields. A nitronaphthalimide derivative, NMO prepared using a simple synthetic protocol, has been studied as an electrochemical, colorimetric, and turn-on fluorescence probe for H<jats:sub>2<\/jats:sub>S. NMO displayed a detection limit of 9.95\u00a0mM and 4.36\u00a0mM in the UV\u2013visible and colorimetric studies, respectively, whereas the fluorometric and square wave techniques confirmed lower detection limits of 98.4\u00a0\u03bcM and 1.24\u00a0mM, correspondingly. Further, the real-time imaging of HEK293T cells using NMO during stress-induced autophagy is demonstrated.<\/jats:p>\n          <jats:p>\n            <jats:bold>Graphical Abstract<\/jats:bold>\n          <\/jats:p>","DOI":"10.1007\/s43630-025-00739-2","type":"journal-article","created":{"date-parts":[[2025,5,22]],"date-time":"2025-05-22T04:12:44Z","timestamp":1747887164000},"page":"975-990","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A versatile electrochemical, colorimetric, and visible light excitable turn-on fluorescent probe for stress-induced H2S detection"],"prefix":"10.1007","volume":"24","author":[{"given":"M.","family":"Ranjana","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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