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This leads to underestimation of molar absorption coefficient. Using Orange Carotenoid Protein (OCP) as a model system, we demonstrate an alternative approach employing the relative actinometry method and femtosecond transient absorption spectroscopy. By comparing the ground state bleaching bands of OCP and \u03b2-carotene recorded immediately after the excitation, before the onset of decay, we determined a molar absorption coefficient that is representative of the holoprotein. \u03b2-carotene serves as a well-characterized reference compound with photophysics similar to that of OCP, enabling error cancellation. We obtained a value of 110,000 M\n                    <jats:sup>\u2212\u20091<\/jats:sup>\n                    cm\n                    <jats:sup>\u22121<\/jats:sup>\n                    at the absorption maximum, significantly higher than previously reported values (63,000 M\n                    <jats:sup>\u22121<\/jats:sup>\n                    cm\n                    <jats:sup>\u22121<\/jats:sup>\n                    and 87,270 M\n                    <jats:sup>\u22121<\/jats:sup>\n                    cm\n                    <jats:sup>\u2212\u20091<\/jats:sup>\n                    ). The method can also be applied to other proteins where conventional approaches fail due to a significant amount of apoprotein.\n                  <\/jats:p>","DOI":"10.1007\/s43630-026-00967-0","type":"journal-article","created":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T12:48:18Z","timestamp":1785502098000},"page":"2061-2069","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Determining molar absorption coefficients for chromoproteins using relative actinometry with transient absorption spectroscopy: application to Orange Carotenoid Protein"],"prefix":"10.1007","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7608-7702","authenticated-orcid":false,"given":"Stanis\u0142aw","family":"Nizi\u0144ski","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabeth","family":"Hartmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Sliwa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gotard","family":"Burdzi\u0144ski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,7,31]]},"reference":[{"issue":"2","key":"967_CR1","doi-asserted-by":"publisher","first-page":"437","DOI":"10.1016\/0003-9861(86)90020-2","volume":"249","author":"Y Nozaki","year":"1986","unstructured":"Nozaki, Y. 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