{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T10:09:09Z","timestamp":1778321349430,"version":"3.51.4"},"reference-count":45,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T00:00:00Z","timestamp":1778284800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T00:00:00Z","timestamp":1778284800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"the Key Program of Science and Technology in Fujian province, China","award":["No. 2024NZ029027"],"award-info":[{"award-number":["No. 2024NZ029027"]}]},{"name":"the National Key Research and Development Program of China","award":["2023YFD1400200"],"award-info":[{"award-number":["2023YFD1400200"]}]},{"name":"the Fujian Provincial Science and Technology Key Project","award":["2022NZ030014"],"award-info":[{"award-number":["2022NZ030014"]}]},{"name":"the Natural Science Foundation of Fujian province","award":["2022J01125"],"award-info":[{"award-number":["2022J01125"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Stress Biology"],"published-print":{"date-parts":[[2026,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The mitotic exit network (MEN), regulated by the small GTPase Tem1, plays a crucial role in coordinating cytokinesis and cell cycle progression in eukaryotes. In this study, we identified MoTem1, a functional homolog of Saccharomyces cerevisiae Tem1, in the rice blast fungus Magnaporthe oryzae, and investigated its role in mitotic regulation and pathogenesis. Using targeted mutagenesis, we generated a series of mutant strains: \u0394\n                    <jats:italic>Motem1<\/jats:italic>\n                    (knockout),\n                    <jats:italic>MoTem1-OE<\/jats:italic>\n                    (overexpression), as well as\n                    <jats:italic>MoTem1-CA<\/jats:italic>\n                    (constitutively active) and\n                    <jats:italic>MoTem1-DN<\/jats:italic>\n                    (dominant-negative) variants created via single-nucleotide substitutions. Phenotypic characterization revealed that MoTem1\u2019s activity states are critical for fungal growth, development, stress tolerance, and pathogenicity. While \u0394\n                    <jats:italic>Motem1<\/jats:italic>\n                    and\n                    <jats:italic>MoTem1-CA<\/jats:italic>\n                    strains showed reduced virulence, the\n                    <jats:italic>MoTem1-DN<\/jats:italic>\n                    mutant exhibited hypervirulence. Transcriptomic profiling and weighted gene co-expression network analysis (WGCNA) identified chitin synthase\n                    <jats:italic>MoCHS1<\/jats:italic>\n                    as a downstream gene whose expression is directly or indirectly influenced by MoTem1 activity states. Pharmacological inhibition of chitin synthesis using Polyoxin B in\n                    <jats:italic>MoTem1-CA<\/jats:italic>\n                    showed increased sensitivity, confirming a decreased expression of chitin synthase in the\n                    <jats:italic>MoTem1-CA<\/jats:italic>\n                    strain. Subcellular localization studies revealed GTP-dependent spindle pole body (SPB) targeting, with inactive MoTem1 failing to localize to SPBs, while constitutive MEN activation in\n                    <jats:italic>MoTem1-CA<\/jats:italic>\n                    disrupted spindle position checkpoint (SPOC) controls, resulting in multinucleate hyphae and a range of developmental defects. In conclusion, our work establishes MoTem1 not merely as a cell cycle regulator, but as a global upstream factor that influences nuclear division, cell wall integrity, and broadly reshapes the genomic regulatory network to govern development and pathogenesis in\n                    <jats:italic>M. oryzae<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1007\/s44154-026-00310-8","type":"journal-article","created":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T09:19:30Z","timestamp":1778318370000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Mutational analysis of the mitotic exit GTPase MoTem1 reveals its role in development, stress adaptation, pathogenicity and global gene regulation in Magnaporthe oryzae"],"prefix":"10.1007","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-4665-652X","authenticated-orcid":false,"given":"Mengtian","family":"Pei","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuze","family":"Xie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingying","family":"Cao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fan","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zonghua","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Olsson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guo-dong","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ya","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2026,5,9]]},"reference":[{"issue":"12","key":"310_CR1","doi-asserted-by":"publisher","first-page":"1287","DOI":"10.3390\/jof8121287","volume":"8","author":"WM Anjago","year":"2022","unstructured":"Anjago WM, Biregeya J, Shi M, Chen Y, Wang Y, Wang Z, Hong Y, Chen M\u00a0(2022) The calcium chloride responsive type 2c protein phosphatases play synergistic roles in regulating MAPK pathways in Magnaporthe oryzae. J Fungi 8(12):1287. https:\/\/doi.org\/10.3390\/jof8121287","journal-title":"J Fungi"},{"issue":"5","key":"310_CR2","doi-asserted-by":"publisher","first-page":"2163","DOI":"10.1091\/mbc.02-05-0076","volume":"14","author":"C Bachewich","year":"2003","unstructured":"Bachewich C, Thomas DY, Whiteway M (2003) Depletion of a polo-like kinase in Candida albicans activates cyclase-dependent hyphal-like growth. Mol Biol Cell 14(5):2163\u20132180. https:\/\/doi.org\/10.1091\/mbc.02-05-0076","journal-title":"Mol Biol Cell"},{"issue":"11","key":"310_CR3","doi-asserted-by":"publisher","first-page":"815","DOI":"10.1038\/35099020","volume":"2","author":"AJ Bardin","year":"2001","unstructured":"Bardin AJ, Amon A (2001) MEN and SIN: what\u2019s the difference? Nat Rev Mol Cell Biol 2(11):815\u2013826. https:\/\/doi.org\/10.1038\/35099020","journal-title":"Nat Rev Mol Cell Biol"},{"issue":"8","key":"310_CR4","doi-asserted-by":"publisher","first-page":"R301","DOI":"10.1016\/S0960-9822(03)00230-6","volume":"13","author":"GD Bettignies","year":"2003","unstructured":"Bettignies GD, Johnston LH (2003) The mitotic exit network. Curr Biol 13(8):R301. https:\/\/doi.org\/10.1016\/S0960-9822(03)00230-6","journal-title":"Curr Biol"},{"issue":"1","key":"310_CR5","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3390\/jof9010001","volume":"9","author":"J Biregeya","year":"2022","unstructured":"Biregeya J, Anjago WM, Pan S, Zhang R, Yang Z, Chen M, Felix A, Xu H, Lin Y, Nkurikiyimfura O, Abubakar YS, Wang Z, Tang W\u00a0(2022) Type 2c protein phosphatases MoPtc5 and MoPtc7 are crucial for multiple stress tolerance, conidiogenesis and pathogenesis of Magnaporthe oryzae. J Fungi 9(1):1. https:\/\/doi.org\/10.3390\/jof9010001","journal-title":"J Fungi"},{"issue":"11","key":"310_CR6","doi-asserted-by":"publisher","first-page":"e1000202","DOI":"10.1371\/journal.ppat.1000202","volume":"4","author":"J Chen","year":"2008","unstructured":"Chen J, Zheng W, Zheng S, Zhang D, Sang W, Chen X, Li G, Lu G, Wang Z\u00a0(2008) Rac1 Is Required for Pathogenicity and Chm1-Dependent Conidiogenesis in Rice Fungal Pathogen Magnaporthe grisea. PLoS Pathog 4(11):e1000202. https:\/\/doi.org\/10.1371\/journal.ppat.1000202. (Edited by A. Andrianopoulos)","journal-title":"PLoS Pathog"},{"issue":"9","key":"310_CR7","doi-asserted-by":"publisher","first-page":"1093","DOI":"10.1111\/mpp.13356","volume":"24","author":"X Chen","year":"2023","unstructured":"Chen X, Pan S, Bai H, Fan J, Batool W, Shabbir A, Han Y, Zheng H, Lu G, Lin L, Tang W, Wang Z\u00a0(2023) A nonclassically secreted effector of Magnaporthe oryzae targets host nuclei and plays important roles in fungal growth and plant infection. Mol Plant Pathol 24(9):1093\u20131106. https:\/\/doi.org\/10.1111\/mpp.13356","journal-title":"Mol Plant Pathol"},{"issue":"4","key":"310_CR8","doi-asserted-by":"publisher","first-page":"414","DOI":"10.1111\/j.1364-3703.2011.00783.x","volume":"13","author":"R Dean","year":"2012","unstructured":"Dean R, Van Kan JA, Pretorius ZA, Hammond-Kosack KE, Di Pietro A, Spanu PD, Rudd JJ, Dickman M, Kahmann R, Ellis J, Foster GD\u00a0(2012) The top 10 fungal pathogens in molecular plant pathology. Mol Plant Pathol 13(4):414\u2013430. https:\/\/doi.org\/10.1111\/j.1364-3703.2011.00783.x","journal-title":"Mol Plant Pathol"},{"issue":"25","key":"310_CR9","doi-asserted-by":"publisher","first-page":"3991","DOI":"10.1091\/mbc.E16-08-0563","volume":"27","author":"JE Falk","year":"2016","unstructured":"Falk JE, Campbell IW, Joyce K, Whalen J, Seshan A, Amon A\u00a0(2016) LTE1 promotes exit from mitosis by multiple mechanisms. Mol Biol Cell 27(25):3991\u20134001. https:\/\/doi.org\/10.1091\/mbc.E16-08-0563. (Edited by D.J. Lew)","journal-title":"Mol Biol Cell"},{"issue":"1","key":"310_CR10","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1007\/s44154-022-00063-0","volume":"2","author":"W Feng","year":"2022","unstructured":"Feng W, Wang J, Liu X, Wu H, Liu M, Zhang H, Zheng X, Wang P, Zhang Z\u00a0(2022) Distinctive phosphorylation pattern during mitotic exit network (MEN) regulation is important for the development and pathogenicity of Magnaporthe oryzae. Stress Biol 2(1):41. https:\/\/doi.org\/10.1007\/s44154-022-00063-0","journal-title":"Stress Biol"},{"issue":"7","key":"310_CR11","doi-asserted-by":"publisher","first-page":"582","DOI":"10.1016\/j.tim.2017.12.007","volume":"26","author":"J Fernandez","year":"2018","unstructured":"Fernandez J, Orth K (2018) Rise of a cereal killer: the biology of Magnaporthe oryzae biotrophic growth. Trends Microbiol 26(7):582\u2013597. https:\/\/doi.org\/10.1016\/j.tim.2017.12.007","journal-title":"Trends Microbiol"},{"issue":"17","key":"310_CR12","doi-asserted-by":"publisher","first-page":"3081","DOI":"10.1091\/mbc.e10-12-1006","volume":"22","author":"MR Finlayson","year":"2011","unstructured":"Finlayson MR, Helfer-Hungerb\u00fchler AK, Philippsen P (2011) Regulation of exit from mitosis in multinucleate Ashbya gossypii cells relies on a minimal network of genes. Mol Biol Cell 22(17):3081\u20133093. https:\/\/doi.org\/10.1091\/mbc.e10-12-1006","journal-title":"Mol Biol Cell"},{"issue":"4","key":"310_CR13","doi-asserted-by":"publisher","first-page":"1909","DOI":"10.1111\/nph.15728","volume":"222","author":"F Fukada","year":"2019","unstructured":"Fukada F, Kodama S, Nishiuchi T, Kajikawa N, Kubo Y\u00a0(2019) Plant pathogenic fungi Colletotrichum and Magnaporthe share a common G1 phase monitoring strategy for proper appressorium development. New Phytol 222(4):1909\u20131923. https:\/\/doi.org\/10.1111\/nph.15728","journal-title":"New Phytol"},{"issue":"9","key":"310_CR14","doi-asserted-by":"publisher","first-page":"2530","DOI":"10.1105\/tpc.15.00179","volume":"27","author":"F Fukada","year":"2015","unstructured":"Fukada F, Kubo Y (2015) Colletotrichum orbiculare regulates cell cycle G1\/S progression via a two-component GAP and a GTPase to establish plant infection. Plant Cell 27(9):2530\u20132544. https:\/\/doi.org\/10.1105\/tpc.15.00179","journal-title":"Plant Cell"},{"issue":"32","key":"310_CR15","doi-asserted-by":"publisher","first-page":"28439","DOI":"10.1074\/jbc.M202540200","volume":"277","author":"M Geymonat","year":"2002","unstructured":"Geymonat M, Spanos A, Smith SJ, Wheatley E, Rittinger K, Johnston LH, Sedgwick SG\u00a0(2002) Control of mitotic exit in budding yeast. J Biol Chem 277(32):28439\u201328445. https:\/\/doi.org\/10.1074\/jbc.M202540200","journal-title":"J Biol Chem"},{"issue":"4837","key":"310_CR16","doi-asserted-by":"publisher","first-page":"288","DOI":"10.1126\/science.239.4837.288","volume":"239","author":"JE Hamer","year":"1988","unstructured":"Hamer JE, Howard RJ, Chumley FG, Valent B\u00a0(1988) A mechanism for surface attachment in spores of a plant pathogenic fungus. Science 239(4837):288\u2013290. https:\/\/doi.org\/10.1126\/science.239.4837.288","journal-title":"Science"},{"issue":"3","key":"310_CR17","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1016\/j.tcb.2013.09.010","volume":"24","author":"M Hotz","year":"2014","unstructured":"Hotz M, Barral Y (2014) The mitotic exit network: new turns on old pathways. Trends Cell Biol 24(3):145\u2013152. https:\/\/doi.org\/10.1016\/j.tcb.2013.09.010","journal-title":"Trends Cell Biol"},{"issue":"1","key":"310_CR18","doi-asserted-by":"publisher","first-page":"26677","DOI":"10.1038\/s41598-025-11673-9","volume":"15","author":"B Ibrahim","year":"2025","unstructured":"Ibrahim B (2025) A minimal deterministic model reveals integration of spindle assembly and position checkpoints in mitosis. Sci Rep 15(1):26677. https:\/\/doi.org\/10.1038\/s41598-025-11673-9","journal-title":"Sci Rep"},{"issue":"6","key":"310_CR19","doi-asserted-by":"publisher","first-page":"1956","DOI":"10.1016\/j.jia.2024.01.010","volume":"23","author":"Y Jiang","year":"2024","unstructured":"Jiang Y,\u00a0Wang R, Du LL, Wang XY, Zhang X, Qi PF, Wu QF, Peng BY, WangZH, Wang M, Li\u00a0Y (2024) The DNA damage repair complex MoMMS21\u2013MoSMC5 is required for infection-related development and pathogenicity of Magnaporthe oryzae. J Integr Agric 23(6):1956\u20131966. https:\/\/doi.org\/10.1016\/j.jia.2024.01.010","journal-title":"J Integr Agric"},{"issue":"2","key":"310_CR20","doi-asserted-by":"publisher","first-page":"706","DOI":"10.1105\/tpc.106.046300","volume":"19","author":"P Kankanala","year":"2007","unstructured":"Kankanala P, Czymmek K, Valent B (2007) Roles for Rice membrane dynamics and plasmodesmata during biotrophic invasion by the blast fungus. Plant Cell 19(2):706\u2013724. https:\/\/doi.org\/10.1105\/tpc.106.046300","journal-title":"Plant Cell"},{"issue":"2","key":"310_CR21","doi-asserted-by":"publisher","first-page":"e1002526","DOI":"10.1371\/journal.ppat.1002526","volume":"8","author":"L-A Kong","year":"2012","unstructured":"Kong LA, Yang J, Li GT, Qi LL, Zhang YJ, Wang CF, Zhao WS, Xu JR, Peng YL\u00a0(2012) Different chitin synthase genes are required for various developmental and plant infection processes in the rice blast fungus Magnaporthe oryzae. PLoS Pathog 8(2):e1002526. https:\/\/doi.org\/10.1371\/journal.ppat.1002526","journal-title":"PLoS Pathog"},{"issue":"2","key":"310_CR22","doi-asserted-by":"publisher","first-page":"328","DOI":"10.1111\/mpp.12523","volume":"19","author":"C Li","year":"2018","unstructured":"Li C, Cao S, Zhang C, Zhang Y, Zhang Q, Xu JR, Wang C\u00a0(2018) MoCDC14 is important for septation during conidiation and appressorium formation in Magnaporthe oryzae. Mol Plant Pathol 19(2):328\u2013340. https:\/\/doi.org\/10.1111\/mpp.12523","journal-title":"Mol Plant Pathol"},{"issue":"14","key":"310_CR23","doi-asserted-by":"publisher","first-page":"2179","DOI":"10.3390\/cells11142179","volume":"11","author":"Y Li","year":"2022","unstructured":"Li Y, Song K (2022) The N-terminal domain of Bfa1 coordinates mitotic exit independent of GAP activity in Saccharomyces cerevisiae. Cells 11(14):2179. https:\/\/doi.org\/10.3390\/cells11142179","journal-title":"Cells"},{"issue":"4","key":"310_CR24","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1006\/meth.2001.1262","volume":"25","author":"KJ Livak","year":"2001","unstructured":"Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394CT method. Methods 25(4):402\u2013408. https:\/\/doi.org\/10.1006\/meth.2001.1262","journal-title":"Methods"},{"issue":"2","key":"310_CR25","doi-asserted-by":"publisher","first-page":"219","DOI":"10.4161\/cc.11.2.18857","volume":"11","author":"F Meitinger","year":"2012","unstructured":"Meitinger F, Palani S, Pereira G (2012) The power of MEN in cytokinesis. Cell Cycle 11(2):219\u2013228. https:\/\/doi.org\/10.4161\/cc.11.2.18857","journal-title":"Cell Cycle"},{"issue":"3","key":"310_CR26","doi-asserted-by":"publisher","first-page":"e1011255","DOI":"10.1371\/journal.ppat.1011255","volume":"19","author":"P Miao","year":"2023","unstructured":"Miao P, Mao X, Chen S, Abubakar YS, Li Y, Zheng W, Zhou J, Wang Z, Zheng H\u00a0(2023) The mitotic exit mediated by small GTPase Tem1 is essential for the pathogenicity of Fusarium graminearum. PLoS Pathog 19(3):e1011255. https:\/\/doi.org\/10.1371\/journal.ppat.1011255. (Edited by J-R Xu)","journal-title":"PLoS Pathog"},{"key":"310_CR27","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1016\/j.fgb.2014.06.007","volume":"69","author":"SW Milne","year":"2014","unstructured":"Milne SW, Cheetham J, Lloyd D, Shaw S, Moore K, Paszkiewicz KH, Aves SJ, Bates S\u00a0(2014) Role of Candida albicans Tem1 in mitotic exit and cytokinesis. Fungal Genet Biol 69:84\u201395. https:\/\/doi.org\/10.1016\/j.fgb.2014.06.007","journal-title":"Fungal Genet Biol"},{"issue":"1","key":"310_CR28","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1038\/35048096","volume":"2","author":"EA Nigg","year":"2001","unstructured":"Nigg EA (2001) Mitotic kinases as regulators of cell division and its checkpoints. Nat Rev Mol Cell Biol 2(1):21\u201332. https:\/\/doi.org\/10.1038\/35048096","journal-title":"Nat Rev Mol Cell Biol"},{"key":"310_CR29","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1146\/annurev.py.18.090180.001123","volume":"18","author":"SH Ou","year":"1980","unstructured":"Ou SH (1980) Pathogen variability and host resistance in rice blast disease. Annu Rev Phytopathol 18:167\u2013187. https:\/\/doi.org\/10.1146\/annurev.py.18.090180.001123","journal-title":"Annu Rev Phytopathol"},{"issue":"10","key":"310_CR30","doi-asserted-by":"publisher","first-page":"e01590-24","DOI":"10.1128\/mbio.01590-24","volume":"15","author":"M Pei","year":"2024","unstructured":"Pei M, Abubakar YS, Ali H, Lin L, Dou X, Lu G, Wang Z, Olsson S, Li Y\u00a0(2024) Whole genome regulatory effect of MoISW2 and consequences for the evolution of the rice plant pathogenic fungus Magnaporthe oryzae. mBio 15(10):e0159024. https:\/\/doi.org\/10.1128\/mbio.01590-24. (Edited by F.M. Winston)","journal-title":"Mbio"},{"key":"310_CR31","doi-asserted-by":"publisher","first-page":"804","DOI":"10.1126\/science.327.5967.804","volume":"327","author":"E Pennisi","year":"2010","unstructured":"Pennisi E (2010) Armed and Dangerous. Science 327:804\u2013805. https:\/\/doi.org\/10.1126\/science.327.5967.804","journal-title":"Science"},{"issue":"4","key":"310_CR32","doi-asserted-by":"publisher","first-page":"196","DOI":"10.1080\/21541248.2015.1109023","volume":"6","author":"I Scarfone","year":"2015","unstructured":"Scarfone I, Piatti S (2015) Coupling spindle position with mitotic exit in budding yeast: The multifaceted role of the small GTPase Tem1. Small GTPases 6(4):196\u2013201. https:\/\/doi.org\/10.1080\/21541248.2015.1109023","journal-title":"Small GTPases"},{"key":"310_CR33","doi-asserted-by":"publisher","first-page":"1519","DOI":"10.1101\/gad.11.12.1519","volume":"11","author":"S Schmidt","year":"1997","unstructured":"Schmidt S, Sohrmann M, Hofmann K, Woollard A, Simanis V\u00a0(1997) The Spglp GTPase is an essential, dosage dependent reducer of septum formation in Schizosaccharomyces pombe. Genes Dev 11:1519\u20131534. https:\/\/doi.org\/10.1101\/gad.11.12.1519","journal-title":"Genes Dev"},{"key":"310_CR34","doi-asserted-by":"publisher","unstructured":"Shirayama M, Matsui Y (1994) The Yeast TEMi Gene, Which Encodes a GTP-Binding Protein, Is Involved in Termination of M Phase. Mol Cell Biol 14(11):7476\u20137482. https:\/\/doi.org\/10.1128\/mcb.14.11.7476-7482.1994","DOI":"10.1128\/mcb.14.11.7476-7482.1994"},{"issue":"1","key":"310_CR35","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1101\/gad.12.1.84","volume":"12","author":"M Sohrmann","year":"1998","unstructured":"Sohrmann M, Schmidt S, Hagan I, Simanis V\u00a0(1998) Asymmetric segregation on spindle poles of the Schizosaccharomyces pombe septum-inducing protein kinase Cdc7p. Genes Dev 12(1):84\u201394. https:\/\/doi.org\/10.1101\/gad.12.1.84","journal-title":"Genes Dev"},{"issue":"7","key":"310_CR36","doi-asserted-by":"publisher","first-page":"931","DOI":"10.1093\/jxb\/42.7.931","volume":"43","author":"P Sundin","year":"1991","unstructured":"Sundin P, Olsson S, Odham G (1991) Degradation of chitotetraose to chitobiose in the axenic rape rhizosphere. J Exp Bot 43(7):931\u2013934. https:\/\/doi.org\/10.1093\/jxb\/42.7.931","journal-title":"J Exp Bot"},{"issue":"7","key":"310_CR37","doi-asserted-by":"publisher","first-page":"3022","DOI":"10.1093\/molbev\/msab120","volume":"38","author":"K Tamura","year":"2021","unstructured":"Tamura K, Stecher G, Kumar S, Battistuzzi FU (2021) MEGA11: molecular evolutionary genetics analysis version 11. Mol Biol Evol 38(7):3022\u20133027. https:\/\/doi.org\/10.1093\/molbev\/msab120","journal-title":"Mol Biol Evol"},{"issue":"4","key":"310_CR38","doi-asserted-by":"publisher","first-page":"599","DOI":"10.1083\/jcb.201007044","volume":"192","author":"M Valerio-Santiago","year":"2011","unstructured":"Valerio-Santiago M, Monje-Casas F (2011) Tem1 localization to the spindle pole bodies is essential for mitotic exit and impairs spindle checkpoint function. J Cell Biol 192(4):599\u2013614. https:\/\/doi.org\/10.1083\/jcb.201007044","journal-title":"J Cell Biol"},{"issue":"3","key":"310_CR39","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1016\/j.fgb.2003.08.005","volume":"40","author":"A Walther","year":"2003","unstructured":"Walther A, Wendland J (2003) Septation and cytokinesis in fungi. Fungal Genet Biol 40(3):187\u2013196. https:\/\/doi.org\/10.1016\/j.fgb.2003.08.005","journal-title":"Fungal Genet Biol"},{"issue":"21","key":"310_CR40","doi-asserted-by":"publisher","first-page":"1379","DOI":"10.1016\/S0960-9822(00)00779-X","volume":"10","author":"Y Wang","year":"2000","unstructured":"Wang Y, Hu F, Elledge SJ (2000) The Bfa1\/Bub2 GAP complex comprises a universal checkpoint required to prevent mitotic exit. Curr Biol 10(21):1379\u20131382. https:\/\/doi.org\/10.1016\/S0960-9822(00)00779-X","journal-title":"Curr Biol"},{"issue":"7","key":"310_CR41","doi-asserted-by":"publisher","first-page":"663","DOI":"10.1016\/j.tim.2021.03.019","volume":"29","author":"RA Wilson","year":"2021","unstructured":"Wilson RA (2021) Magnaporthe oryzae. Trends Microbiol 29(7):663\u2013664. https:\/\/doi.org\/10.1016\/j.tim.2021.03.019","journal-title":"Trends Microbiol"},{"issue":"1","key":"310_CR42","doi-asserted-by":"publisher","first-page":"172","DOI":"10.1105\/tpc.18.00382","volume":"31","author":"C Yang","year":"2019","unstructured":"Yang C, Yu Y, Huang J, Meng F, Pang J, Zhao Q, Islam MA, Xu N, Tian Y, Liu J\u00a0(2019) Binding of the Magnaporthe oryzae chitinase MoChia1 by a rice tetratricopeptide repeat protein allows free chitin to trigger immune responses. Plant Cell 31(1):172\u2013188. https:\/\/doi.org\/10.1105\/tpc.18.00382","journal-title":"Plant Cell"},{"issue":"8","key":"310_CR43","doi-asserted-by":"publisher","first-page":"511","DOI":"10.3390\/jof10080511","volume":"10","author":"H Zhang","year":"2024","unstructured":"Zhang H, Chen Z, Yu Z, Tang L, Gao W, Lu X, Yang J\u00a0(2024) Magnaporthe-unique gene MUG1 is important for fungal appressorial penetration, invasive hyphal extension, and virulence in rice blast fungi. J Fungi 10(8):511. https:\/\/doi.org\/10.3390\/jof10080511","journal-title":"J Fungi"},{"key":"310_CR44","doi-asserted-by":"publisher","first-page":"113","DOI":"10.3389\/fcimb.2019.00113","volume":"9","author":"L Zhang","year":"2019","unstructured":"Zhang L, Zhang D, Chen Y, Ye W, Lin Q, Lu G, Ebbole DJ, Olsson S, Wang Z\u00a0(2019) Magnaporthe oryzae CK2 accumulates in nuclei, nucleoli, at septal pores and forms a large ring structure in appressoria, and is involved in rice blast pathogenesis. Front Cell Infect Microbiol 9:113. https:\/\/doi.org\/10.3389\/fcimb.2019.00113","journal-title":"Front Cell Infect Microbiol"},{"issue":"45","key":"310_CR45","doi-asserted-by":"publisher","first-page":"e2413873121","DOI":"10.1073\/pnas.2413873121","volume":"121","author":"X Zhou","year":"2024","unstructured":"Zhou X, Weng SY, Bell SP, Amon A\u00a0(2024) A noncanonical GTPase signaling mechanism controls exit from mitosis in budding yeast. Proc Natl Acad Sci U S A 121(45):e2413873121. https:\/\/doi.org\/10.1073\/pnas.2413873121","journal-title":"Proc Natl Acad Sci U S A"}],"container-title":["Stress Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s44154-026-00310-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s44154-026-00310-8","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s44154-026-00310-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T09:19:31Z","timestamp":1778318371000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s44154-026-00310-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,9]]},"references-count":45,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,12]]}},"alternative-id":["310"],"URL":"https:\/\/doi.org\/10.1007\/s44154-026-00310-8","relation":{},"ISSN":["2097-4418","2731-0450"],"issn-type":[{"value":"2097-4418","type":"print"},{"value":"2731-0450","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,9]]},"assertion":[{"value":"14 October 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 February 2026","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 April 2026","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"9 May 2026","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"All the authors have given their consent for publication of this manuscript by\n                      Stress Biology\n                      .","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"Z.W. is a member of the editorial board but was not involved in the journal's review, or any decisions, related to this submission.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"38"}}