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Furthermore, derivatized steroids analyzed by ME-SALDI\/MS exhibited significantly higher ion yields than those obtained by conventional MALDI\/MS or SALDI\/MS, demonstrating a synergistic enhancement of desorption and ionization efficiency. ME-SALDI\/MS imaging of porcine adrenal gland tissue enabled visualization of multiple derivatized steroids with high ion yields\u2014including progesterone, corticosterone, and cortisol\u2014reflecting their biosynthetic localization across cortical zones. Collectively, these results highlight the potential of Pt thin film\u2013based SALDI and ME-SALDI\/MS as robust tools that provide high ion yields for imaging of steroids and other biomolecules with inherently low desorption and ionization efficiency.<\/jats:p>\n                  <jats:p>\n                    <jats:bold>Graphical abstract<\/jats:bold>\n                  <\/jats:p>","DOI":"10.1007\/s44211-026-00935-9","type":"journal-article","created":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T23:45:54Z","timestamp":1782344754000},"page":"855-864","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Platinum thin film\u2013based matrix-enhanced surface-assisted laser desorption\/ionization mass spectrometry imaging for improved ion yields of adrenocortical steroids"],"prefix":"10.1007","volume":"42","author":[{"given":"Riko","family":"Takata","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigehiro","family":"Karashima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kokoro","family":"Okawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akio","family":"Miyazato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumitake","family":"Usui-Kawanishi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reina","family":"Nakanishi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsuya","family":"Murakami","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsushi","family":"Hashimoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Kawasaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3642-2278","authenticated-orcid":false,"given":"Issey","family":"Osaka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,6,25]]},"reference":[{"key":"935_CR1","doi-asserted-by":"publisher","first-page":"1854","DOI":"10.1021\/pr901008d","volume":"9","author":"S Rauser","year":"2010","unstructured":"S. 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