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Biotechnol."],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Adaptive immune diversity is shaped by the complex organization of T cell receptor (TCR) and B cell receptor (BCR) repertoires across tissues. However, a systematic cross-tissue immune repertoire profiling in healthy pigs is still lacking. In this study, we used 3 14-day-old Duroc\u2009\u00d7\u2009Landrace\u2009\u00d7\u2009Yorkshire (DLY) crossbred piglets, all of which were male. After sample purification, a total of 71,731 cells were retained. We established single-cell profiling integrating paired BCR and TCR repertoires across 11 immune-associated tissues using single-cell RNA sequencing combined with V(D)J sequencing based on DLY crossbred piglets. Diverse populations of immune cells were identified, with significant tissue-specific heterogeneity being revealed in lymphatic, mucosal, and circulatory organs. Immune repertoire analysis demonstrated biased V(D)J gene usage, conserved complementarity-determining region 3 (CDR3) length distributions, preferential receptor pairing patterns, and extensive clonotype diversity. Cross-tissue analyses further revealed coordinated clonotype sharing and distinct clonal expansion patterns among immune cell subsets. In addition, using the Pig-specific V(D)J primers we designed, the sequencing results have achieved a modest improvement in the detection of immunoglobulin alleles compared to previous studies. It is expected to provide a more reliable technical solution for related research. Collectively, this work provides a comprehensive reference profiling of the healthy DLY crossbred piglet adaptive immune system and offers valuable resources for future studies of porcine immunology, infectious diseases, and vaccine responses.<\/jats:p>","DOI":"10.1007\/s44307-026-00139-2","type":"journal-article","created":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T02:25:53Z","timestamp":1791512753000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A single-cell profile of the porcine immune repertoire: concurrent profiling of T cell receptor and B cell receptor V(D)J sequences in health"],"prefix":"10.1007","volume":"4","author":[{"given":"Hao","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qixin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"He","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Panfei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Geng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rufei","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feifei","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0311-5474","authenticated-orcid":false,"given":"Yongchang","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,10,9]]},"reference":[{"key":"139_CR1","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1038\/icb.2010.117","volume":"89","author":"CK Baumgartner","year":"2011","unstructured":"Baumgartner CK, Malherbe LP. 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