{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T01:00:06Z","timestamp":1790643606664,"version":"4.1.0"},"reference-count":29,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T00:00:00Z","timestamp":1790553600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T00:00:00Z","timestamp":1790553600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"the Institute for Food and Health Science, Yazuya Co., Ltd.","award":["N\/A"],"award-info":[{"award-number":["N\/A"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Food Sci Anim Resour"],"published-print":{"date-parts":[[2026,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>This exploratory study aimed to compare estimated soluble protein\/peptide yields and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging and angiotensin-converting enzyme (ACE)-inhibitory activities after in vitro digestion of eight tissues from wild-caught sika deer and wild boar and corresponding cattle and pig tissues, with relevance to value-added utilization in Japan. Eight tissues were obtained from sika deer and wild boar (n\u2009=\u20095 each) and commercially sourced cattle and pigs (n\u2009=\u20093 per tissue per species). Heated samples underwent gastric and intestinal digestion. DPPH and ACE activities were expressed as IC\u2085\u2080 values and analyzed using Kruskal\u2013Wallis tests followed by Dunn\u2019s tests with Holm adjustment. Estimated soluble protein\/peptide yields differed among species groups in six tissues. Wild boar had higher yields in the stomach than sika deer, in the liver than sika deer and cattle, and in the round than pigs, but a lower yield in the small intestine than pigs. Sika deer spleen had a lower yield than wild boar and cattle spleens. DPPH IC\u2085\u2080 values were lower in wild boar liver than cattle liver and in wild boar loin than sika deer and cattle loin. ACE IC\u2085\u2080 was lower in sika deer spleen than wild boar spleen. Differences were tissue dependent, and estimated soluble protein\/peptide yield did not consistently correspond to either activity. Wild boar liver and loin and sika deer spleen may warrant further characterization as candidates for value-added utilization in Japan.<\/jats:p>","DOI":"10.1007\/s44463-026-00118-0","type":"journal-article","created":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T11:41:50Z","timestamp":1790595710000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Exploratory screening of wild-game tissues in Japan toward value-added utilization: estimated soluble protein\/peptide yields and DPPH radical-scavenging and ACE-inhibitory activities after in vitro digestion"],"prefix":"10.1007","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-0369-6507","authenticated-orcid":false,"given":"Hikaru","family":"Ogi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-1073-3686","authenticated-orcid":false,"given":"Takehiko","family":"Terashima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-7476-5726","authenticated-orcid":false,"given":"Ryo","family":"Mannen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-2640-9118","authenticated-orcid":false,"given":"Naoko","family":"Otsuki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5924-4657","authenticated-orcid":false,"given":"Yoko","family":"Ichikawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,28]]},"reference":[{"key":"118_CR26","doi-asserted-by":"publisher","first-page":"2895","DOI":"10.1021\/jf904204n","volume":"58","author":"E Escudero","year":"2010","unstructured":"Escudero, E., Sentandreu, M. 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Institutional animal ethics review was not required because the tissue samples used in this study were obtained for food use from a commercial supplier or after routine commercial processing, and no animals were captured, transported, slaughtered, or experimentally treated specifically for this study. The sika deer and wild boar tissue samples were obtained from animals captured as part of legally authorized population-management and commercial game-meat utilization activities and routinely processed at a licensed wild-game meat processing facility. Capture, transport, slaughter, and carcass processing were conducted independently of this study by licensed hunters and processing-facility personnel. The authors informed the facility of the eligibility criteria for sex and estimated age but were not involved in capture, transport, slaughter, or carcass processing. Samples were collected after slaughter during routine processing at the facility. Cattle and pig tissues used as comparison samples were purchased from a meat supplier.","order":2,"name":"Ethics","label":"Ethics statement","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"106"}}