{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,26]],"date-time":"2025-03-26T05:46:34Z","timestamp":1742967994500,"version":"3.40.3"},"publisher-location":"New York, NY","reference-count":10,"publisher":"Springer New York","isbn-type":[{"type":"print","value":"9781493923359"},{"type":"electronic","value":"9781493923366"}],"license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015]]},"DOI":"10.1007\/978-1-4939-2336-6_11","type":"book-chapter","created":{"date-parts":[[2015,1,6]],"date-time":"2015-01-06T12:31:04Z","timestamp":1420547464000},"page":"143-172","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Use of ImageJ to Recover Information from Individual Cells in a G Protein-Coupled Receptor Assay"],"prefix":"10.1007","author":[{"given":"Jo\u00e3o R. C.","family":"Trabuco","sequence":"first","affiliation":[]},{"given":"Sofia Aires M.","family":"Martins","sequence":"additional","affiliation":[]},{"given":"Duarte Miguel F.","family":"Prazeres","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2015,12,23]]},"reference":[{"key":"11_CR1","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1038\/nmeth.2019","volume":"9","author":"J Schindelin","year":"2012","unstructured":"Schindelin J, Arganda-Carreras I, Frise E, Kaynig V, Longair M, Pietzsch T, Preibisch S, Rueden C, Saalfeld S, Schmid B, Tinevez J-Y, White DJ, Hartenstein V, Eliceiri K, Tomancak P, Cardona A (2012) Fiji: an open-source platform for biological-image analysis. Nat Methods 9:676\u2013682","journal-title":"Nat Methods"},{"key":"11_CR2","doi-asserted-by":"publisher","first-page":"671","DOI":"10.1038\/nmeth.2089","volume":"9","author":"CA Schneider","year":"2012","unstructured":"Schneider CA, Rasband WS, Eliceiri KW (2012) Historical commentary NIH Image to ImageJ: 25 years of image analysis. Nat Methods 9:671\u2013676","journal-title":"Nat Methods"},{"key":"11_CR3","doi-asserted-by":"publisher","first-page":"372","DOI":"10.1038\/aps.2011.173","volume":"33","author":"R Zhang","year":"2012","unstructured":"Zhang R, Xie X (2012) Tools for GPCR drug discovery. Acta Pharmacol Sin 33:372\u2013384","journal-title":"Acta Pharmacol Sin"},{"key":"11_CR4","doi-asserted-by":"publisher","first-page":"566","DOI":"10.1016\/j.tibtech.2012.07.004","volume":"30","author":"SAM Martins","year":"2012","unstructured":"Martins SAM, Trabuco JRC, Monteiro GA, Chu V, Conde JP, Prazeres DMF (2012) Towards the miniaturization of GPCR-based live-cell screening assays. Trends Biotechnol 30:566\u2013574","journal-title":"Trends Biotechnol"},{"key":"11_CR5","first-page":"367","volume":"8","author":"K Liu","year":"2010","unstructured":"Liu K, Southall N, Titus SA, Inglese J, Eskay RL, Shinn P, Austin CP, Heilig MA, Zheng W (2010) A multiplex calcium assay for identification of GPCR agonists and antagonists. Assay Drug Dev Technol 8:367\u2013379","journal-title":"Assay Drug Dev Technol"},{"key":"11_CR6","doi-asserted-by":"publisher","first-page":"697","DOI":"10.1038\/nmeth.2084","volume":"9","author":"KW Eliceiri","year":"2012","unstructured":"Eliceiri KW, Berthold MR, Goldberg IG, Ib\u00e1\u00f1ez L, Manjunath BS, Martone ME, Murphy RF, Peng H, Plant AL, Roysam B, Stuurman N, Stuurmann N, Swedlow JR, Tomancak P, Carpenter AE (2012) Biological imaging software tools. Nat Methods 9:697\u2013710","journal-title":"Nat Methods"},{"key":"11_CR7","doi-asserted-by":"publisher","first-page":"690","DOI":"10.1038\/nmeth.2075","volume":"9","author":"F de Chaumont","year":"2012","unstructured":"de Chaumont F, Dallongeville S, Chenouard N, Herv\u00e9 N, Pop S, Provoost T, Meas-Yedid V, Pankajakshan P, Lecomte T, Le Montagner Y, Lagache T, Dufour A, Olivo-Marin J-C (2012) Icy: an open bioimage informatics platform for extended reproducible research. Nat Methods 9:690\u2013696","journal-title":"Nat Methods"},{"key":"11_CR8","doi-asserted-by":"publisher","first-page":"683","DOI":"10.1038\/nmeth.2047","volume":"9","author":"P Kankaanp\u00e4\u00e4","year":"2012","unstructured":"Kankaanp\u00e4\u00e4 P, Paavolainen L, Tiitta S, Karjalainen M, P\u00e4iv\u00e4rinne J, Nieminen J, Marjom\u00e4ki V, Heino J, White DJ (2012) BioImageXD: an open, general-purpose and high-throughput image-processing platform. Nat Methods 9:683\u2013689","journal-title":"Nat Methods"},{"key":"11_CR9","doi-asserted-by":"publisher","first-page":"661","DOI":"10.1038\/nmeth.2082","volume":"9","author":"A Cardona","year":"2012","unstructured":"Cardona A, Tomancak P (2012) Current challenges in open-source bioimage informatics. Nat Methods 9:661\u2013665","journal-title":"Nat Methods"},{"key":"11_CR10","doi-asserted-by":"publisher","first-page":"232","DOI":"10.1016\/j.bios.2013.08.037","volume":"52","author":"SAM Martins","year":"2014","unstructured":"Martins SAM, Moulas G, Trabuco JRC, Monteiro GA, Chu V, Conde JP, Prazeres DMF (2014) Monitoring intracellular calcium in response to GPCR activation using thin-film silicon photodiodes with integrated fluorescence filters. Biosens Bioelectron 52:232\u2013238","journal-title":"Biosens Bioelectron"}],"container-title":["Methods in Molecular Biology","G Protein-Coupled Receptor Screening Assays"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-4939-2336-6_11","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,21]],"date-time":"2020-05-21T04:15:46Z","timestamp":1590034546000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/978-1-4939-2336-6_11"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015]]},"ISBN":["9781493923359","9781493923366"],"references-count":10,"URL":"https:\/\/doi.org\/10.1007\/978-1-4939-2336-6_11","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"type":"print","value":"1064-3745"},{"type":"electronic","value":"1940-6029"}],"subject":[],"published":{"date-parts":[[2015]]},"assertion":[{"value":"23 December 2015","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}