{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,10]],"date-time":"2025-06-10T04:02:44Z","timestamp":1749528164217,"version":"3.41.0"},"publisher-location":"Cham","reference-count":52,"publisher":"Springer Nature Switzerland","isbn-type":[{"value":"9783031935534","type":"print"},{"value":"9783031935541","type":"electronic"}],"license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025]]},"DOI":"10.1007\/978-3-031-93554-1_41","type":"book-chapter","created":{"date-parts":[[2025,6,9]],"date-time":"2025-06-09T13:44:52Z","timestamp":1749476692000},"page":"455-467","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Design of a Double-Chamber Perfusion Bioreactor for Osteochondral Tissue Repair: An Innovative Concept"],"prefix":"10.1007","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2610-1005","authenticated-orcid":false,"given":"Carla","family":"Moura","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0009-0009-7329-2511","authenticated-orcid":false,"given":"M\u00e1rio","family":"Loureiro","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0009-0003-9220-3509","authenticated-orcid":false,"given":"Diogo","family":"Palaio","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8217-8600","authenticated-orcid":false,"given":"Rachel","family":"Cordeiro","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5857-9903","authenticated-orcid":false,"given":"Ant\u00f3nio Carvalho","family":"Santos","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0012-4380","authenticated-orcid":false,"given":"C\u00e2ndida","family":"Mal\u00e7a","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2025,6,10]]},"reference":[{"key":"41_CR1","doi-asserted-by":"publisher","DOI":"10.1016\/J.COMPOSITESB.2023.110736","volume":"259","author":"X Niu","year":"2023","unstructured":"Niu, X., Xu, Z., Di, M., et al.: Bioreactor strategies for tissue-engineered osteochondral constructs: advantages, present situations and future trends. Compos. Part B Eng. 259, 110736 (2023). https:\/\/doi.org\/10.1016\/J.COMPOSITESB.2023.110736","journal-title":"Compos. Part B Eng."},{"key":"41_CR2","doi-asserted-by":"publisher","unstructured":"Zhou, L., Gjvm, V.O., Malda, J., et al.: Innovative tissue-engineered strategies for osteochondral defect repair and regeneration: current progress and challenges. Adv. Healthc. Mater. 9 (2020). https:\/\/doi.org\/10.1002\/ADHM.202001008","DOI":"10.1002\/ADHM.202001008"},{"key":"41_CR3","doi-asserted-by":"publisher","first-page":"766","DOI":"10.1089\/TEN.TEB.2021.0101","volume":"28","author":"R Choe","year":"2022","unstructured":"Choe, R., Devoy, E., Jabari, E., et al.: Biomechanical aspects of osteochondral regeneration: implications and strategies for three-dimensional bioprinting. Tissue Eng. Part B Rev. 28, 766\u2013788 (2022). https:\/\/doi.org\/10.1089\/TEN.TEB.2021.0101","journal-title":"Tissue Eng. Part B Rev."},{"key":"41_CR4","doi-asserted-by":"publisher","first-page":"1900078","DOI":"10.1002\/BIOT.201900078","volume":"15","author":"JC Silva","year":"2020","unstructured":"Silva, J.C., Moura, C.S., Borrecho, G., et al.: Extruded bioreactor perfusion culture supports the chondrogenic differentiation of human mesenchymal stem\/stromal cells in 3D porous poly(\u025b-caprolactone) scaffolds. Biotechnol. J. 15, 1900078 (2020). https:\/\/doi.org\/10.1002\/BIOT.201900078","journal-title":"Biotechnol. J."},{"key":"41_CR5","doi-asserted-by":"publisher","first-page":"756","DOI":"10.1016\/j.jbiosc.2020.01.004","volume":"129","author":"CS Moura","year":"2020","unstructured":"Moura, C.S., Silva, J.C., Faria, S., et al.: Chondrogenic differentiation of mesenchymal stem\/stromal cells on 3D porous poly (\u03b5-caprolactone) scaffolds: effects of material alkaline treatment and chondroitin sulfate supplementation. J. Biosci. Bioeng. 129, 756\u2013764 (2020). https:\/\/doi.org\/10.1016\/j.jbiosc.2020.01.004","journal-title":"J. Biosci. Bioeng."},{"key":"41_CR6","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1016\/J.JSS.2007.12.788","volume":"149","author":"S Janjanin","year":"2008","unstructured":"Janjanin, S., Li, W.J., Morgan, M.T., et al.: Mold-shaped, nanofiber scaffold-based cartilage engineering using human mesenchymal stem cells and bioreactor. J. Surg. Res. 149, 47\u201356 (2008). https:\/\/doi.org\/10.1016\/J.JSS.2007.12.788","journal-title":"J. Surg. Res."},{"key":"41_CR7","doi-asserted-by":"publisher","first-page":"1645","DOI":"10.1007\/S10529-013-1248-9","volume":"35","author":"LX guo","year":"2013","unstructured":"guo, L.X., kui, J.H.: Preparation of an osteochondral composite with mesenchymal stem cells as the single-cell source in a double-chamber bioreactor. Biotechnol. Lett. 35, 1645\u20131653 (2013). https:\/\/doi.org\/10.1007\/S10529-013-1248-9","journal-title":"Biotechnol. Lett."},{"key":"41_CR8","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1016\/J.JOT.2021.07.008","volume":"30","author":"C Ai","year":"2021","unstructured":"Ai, C., Lee, Y.H.D., Tan, X.H., et al.: Osteochondral tissue engineering: perspectives for clinical application and preclinical development. J. Orthop. Transl. 30, 93\u2013102 (2021). https:\/\/doi.org\/10.1016\/J.JOT.2021.07.008","journal-title":"J. Orthop. Transl."},{"key":"41_CR9","doi-asserted-by":"publisher","first-page":"318","DOI":"10.2174\/157488809789649205","volume":"4","author":"C Vinatier","year":"2009","unstructured":"Vinatier, C., Bouffi, C., Merceron, C., et al.: Cartilage tissue engineering: towards a biomaterial-assisted mesenchymal stem cell therapy. Curr. Stem Cell Res. Ther. 4, 318\u2013329 (2009). https:\/\/doi.org\/10.2174\/157488809789649205","journal-title":"Curr. Stem Cell Res. Ther."},{"key":"41_CR10","doi-asserted-by":"publisher","first-page":"1078","DOI":"10.1002\/JBM.A.32387","volume":"92A","author":"BA Harley","year":"2010","unstructured":"Harley, B.A., Lynn, A.K., Wissner-Gross, Z., et al.: Design of a multiphase osteochondral scaffold III: fabrication of layered scaffolds with continuous interfaces. J. Biomed. Mater. Res. A. 92A, 1078\u20131093 (2010). https:\/\/doi.org\/10.1002\/JBM.A.32387","journal-title":"J. Biomed. Mater. Res. A"},{"key":"41_CR11","doi-asserted-by":"publisher","first-page":"490","DOI":"10.1016\/J.ACTBIO.2008.01.003","volume":"4","author":"BP Kanungo","year":"2008","unstructured":"Kanungo, B.P., Silva, E., Van, V.K., Gibson, L.J.: Characterization of mineralized collagen\u2013glycosaminoglycan scaffolds for bone regeneration. Acta Biomater. 4, 490\u2013503 (2008). https:\/\/doi.org\/10.1016\/J.ACTBIO.2008.01.003","journal-title":"Acta Biomater."},{"key":"41_CR12","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1089\/TEN.TEB.2008.0388","volume":"15","author":"M Keeney","year":"2009","unstructured":"Keeney, M., Pandit, A.: The osteochondral junction and its repair via bi-phasic tissue engineering scaffolds. Tissue Eng. Part B Rev. 15, 55\u201373 (2009). https:\/\/doi.org\/10.1089\/TEN.TEB.2008.0388","journal-title":"Tissue Eng. Part B Rev."},{"key":"41_CR13","doi-asserted-by":"publisher","first-page":"122","DOI":"10.1016\/J.BIOACTMAT.2023.01.012","volume":"25","author":"L Yu","year":"2023","unstructured":"Yu, L., Cavelier, S., Hannon, B., Wei, M.: Recent development in multizonal scaffolds for osteochondral regeneration. Bioact. Mater. 25, 122\u2013159 (2023). https:\/\/doi.org\/10.1016\/J.BIOACTMAT.2023.01.012","journal-title":"Bioact. Mater."},{"key":"41_CR14","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/J.JCONREL.2022.09.022","volume":"351","author":"AM Maadani","year":"2022","unstructured":"Maadani, A.M., Salahinejad, E.: Performance comparison of PLA- and PLGA-coated porous bioceramic scaffolds: mechanical, biodegradability, bioactivity, delivery and biocompatibility assessments. J. Control. Release. 351, 1\u20137 (2022). https:\/\/doi.org\/10.1016\/J.JCONREL.2022.09.022","journal-title":"J. Control. Release"},{"key":"41_CR15","doi-asserted-by":"publisher","first-page":"2017","DOI":"10.1002\/JBM.B.34542","volume":"108","author":"LF Mellor","year":"2020","unstructured":"Mellor, L.F., Nordberg, R.C., Huebner, P., et al.: Investigation of multiphasic 3D-bioplotted scaffolds for site-specific chondrogenic and osteogenic differentiation of human adipose-derived stem cells for osteochondral tissue engineering applications. J. Biomed. Mater. Res. B Appl. Biomater. 108, 2017\u20132030 (2020). https:\/\/doi.org\/10.1002\/JBM.B.34542","journal-title":"J. Biomed. Mater. Res. B Appl. Biomater."},{"key":"41_CR16","doi-asserted-by":"publisher","first-page":"4830","DOI":"10.1016\/J.BIOACTMAT.2021.05.011","volume":"6","author":"W Wei","year":"2021","unstructured":"Wei, W., Dai, H.: Articular cartilage and osteochondral tissue engineering techniques: recent advances and challenges. Bioact. Mater. 6, 4830\u20134855 (2021). https:\/\/doi.org\/10.1016\/J.BIOACTMAT.2021.05.011","journal-title":"Bioact. Mater."},{"key":"41_CR17","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1007\/S42242-018-0015-0\/FIGURES\/4","volume":"1","author":"M Tamaddon","year":"2018","unstructured":"Tamaddon, M., Wang, L., Liu, Z., Liu, C.: Osteochondral tissue repair in osteoarthritic joints: clinical challenges and opportunities in tissue engineering. Biodes. Manuf. 1, 101\u2013114 (2018). https:\/\/doi.org\/10.1007\/S42242-018-0015-0\/FIGURES\/4","journal-title":"Biodes. Manuf."},{"key":"41_CR18","doi-asserted-by":"publisher","first-page":"2053","DOI":"10.1177\/0363546508328414","volume":"37","author":"K Mithoefer","year":"2009","unstructured":"Mithoefer, K., Mcadams, T., Williams, R.J., et al.: Clinical efficacy of the microfracture technique for articular cartilage repair in the knee: an evidence-based systematic analysis. Am. J. Sports Med. 37, 2053\u20132063 (2009). https:\/\/doi.org\/10.1177\/0363546508328414","journal-title":"Am. J. Sports Med."},{"key":"41_CR19","doi-asserted-by":"publisher","first-page":"423","DOI":"10.1007\/S12178-015-9299-2","volume":"8","author":"S Patil","year":"2015","unstructured":"Patil, S., Tapasvi, S.R.: Osteochondral autografts. Curr. Rev. Musculoskelet. Med. 8, 423\u2013428 (2015). https:\/\/doi.org\/10.1007\/S12178-015-9299-2","journal-title":"Curr. Rev. Musculoskelet. Med."},{"key":"41_CR20","doi-asserted-by":"publisher","first-page":"750","DOI":"10.1016\/J.JBIOMECH.2006.03.008","volume":"40","author":"I Martin","year":"2007","unstructured":"Martin, I., Miot, S., Barbero, A., et al.: Osteochondral tissue engineering. J. Biomech. 40, 750\u2013765 (2007). https:\/\/doi.org\/10.1016\/J.JBIOMECH.2006.03.008","journal-title":"J. Biomech."},{"key":"41_CR21","doi-asserted-by":"publisher","first-page":"7481","DOI":"10.1016\/J.BIOMATERIALS.2005.05.057","volume":"26","author":"Y Martin","year":"2005","unstructured":"Martin, Y., Vermette, P.: Bioreactors for tissue mass culture: design, characterization, and recent advances. Biomaterials. 26, 7481\u20137503 (2005). https:\/\/doi.org\/10.1016\/J.BIOMATERIALS.2005.05.057","journal-title":"Biomaterials"},{"key":"41_CR22","doi-asserted-by":"publisher","first-page":"235","DOI":"10.1263\/JBB.100.235","volume":"100","author":"R P\u00f6rtner","year":"2005","unstructured":"P\u00f6rtner, R., Nagel-Heyer, S., Goepfert, C., et al.: Bioreactor design for tissue engineering. J. Biosci. Bioeng. 100, 235\u2013245 (2005). https:\/\/doi.org\/10.1263\/JBB.100.235","journal-title":"J. Biosci. Bioeng."},{"key":"41_CR23","doi-asserted-by":"publisher","first-page":"489","DOI":"10.1263\/JBB.100.489","volume":"100","author":"D Wendt","year":"2005","unstructured":"Wendt, D., Jakob, M., Martin, I.: Bioreactor-based engineering of osteochondral grafts: from model systems to tissue manufacturing. J. Biosci. Bioeng. 100, 489\u2013494 (2005). https:\/\/doi.org\/10.1263\/JBB.100.489","journal-title":"J. Biosci. Bioeng."},{"key":"41_CR24","unstructured":"Moura, C., Trindade, D., Cordeiro, R., et al.: Biorreator de Perfus\u00e3o de C\u00e2mara Dupla (2022)"},{"key":"41_CR25","doi-asserted-by":"publisher","first-page":"468","DOI":"10.1016\/j.copbio.2003.09.001","volume":"14","author":"C Komives","year":"2003","unstructured":"Komives, C., Parker, R.S.: Bioreactor state estimation and control. Curr. Opin. Biotechnol. 14, 468\u2013474 (2003). https:\/\/doi.org\/10.1016\/j.copbio.2003.09.001","journal-title":"Curr. Opin. Biotechnol."},{"key":"41_CR26","doi-asserted-by":"publisher","first-page":"1827","DOI":"10.1089\/TEN.TEA.2013.0500","volume":"20","author":"LE Monfoulet","year":"2014","unstructured":"Monfoulet, L.E., Becquart, P., Marchat, D., et al.: The pH in the microenvironment of human mesenchymal stem cells is a critical factor for optimal osteogenesis in tissue-engineered constructs. Tissue Eng. Part A. 20, 1827\u20131840 (2014). https:\/\/doi.org\/10.1089\/TEN.TEA.2013.0500","journal-title":"Tissue Eng. Part A"},{"key":"41_CR27","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/BFB0000746","volume":"44","author":"R Bliem","year":"1991","unstructured":"Bliem, R., Konopitzky, K., Katinger, H.: Industrial animal cell reactor systems: aspects of selection and evaluation. Adv. Biochem. Eng. Biotechnol. 44, 1\u201326 (1991). https:\/\/doi.org\/10.1007\/BFB0000746","journal-title":"Adv. Biochem. Eng. Biotechnol."},{"key":"41_CR28","doi-asserted-by":"publisher","first-page":"930","DOI":"10.1359\/JBMR.1999.14.6.930","volume":"14","author":"TN McAllister","year":"1999","unstructured":"McAllister, T.N., Frangos, J.A.: Steady and transient fluid shear stress stimulate NO release in osteoblasts through distinct biochemical pathways. J. Bone Miner. Res. 14, 930\u2013936 (1999). https:\/\/doi.org\/10.1359\/JBMR.1999.14.6.930","journal-title":"J. Bone Miner. Res."},{"key":"41_CR29","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1002\/JOR.1100170118","volume":"17","author":"RM Schinagl","year":"1999","unstructured":"Schinagl, R.M., Kurtis, M.S., Ellis, K.D., et al.: Effect of seeding duration on the strength of chondrocyte adhesion to articular cartilage. J. Orthop. Res. 17, 121\u2013129 (1999). https:\/\/doi.org\/10.1002\/JOR.1100170118","journal-title":"J. Orthop. Res."},{"key":"41_CR30","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1016\/J.DEVCEL.2005.12.006","volume":"10","author":"AW Orr","year":"2006","unstructured":"Orr, A.W., Helmke, B.P., Blackman, B.R., Schwartz, M.A.: Mechanisms of mechanotransduction. Dev. Cell. 10, 11\u201320 (2006). https:\/\/doi.org\/10.1016\/J.DEVCEL.2005.12.006","journal-title":"Dev. Cell"},{"key":"41_CR31","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/J.BBRC.2006.07.214","volume":"349","author":"A Liedert","year":"2006","unstructured":"Liedert, A., Kaspar, D., Blakytny, R., et al.: Signal transduction pathways involved in mechanotransduction in bone cells. Biochem. Biophys. Res. Commun. 349, 1\u20135 (2006). https:\/\/doi.org\/10.1016\/J.BBRC.2006.07.214","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"41_CR32","doi-asserted-by":"publisher","first-page":"1497","DOI":"10.1242\/JCS.108.4.1497","volume":"108","author":"MD Buschmann","year":"1995","unstructured":"Buschmann, M.D., Gluzband, Y.A., Grodzinsky, A.J., Hunziker, E.B.: Mechanical compression modulates matrix biosynthesis in chondrocyte\/agarose culture. J. Cell Sci. 108, 1497\u20131508 (1995). https:\/\/doi.org\/10.1242\/JCS.108.4.1497","journal-title":"J. Cell Sci."},{"key":"41_CR33","doi-asserted-by":"publisher","unstructured":"Anderson, D.E., Johnstone, B.: Dynamic mechanical compression of chondrocytes for tissue engineering: a critical review. Front. Bioeng. Biotechnol. 5 (2017). https:\/\/doi.org\/10.3389\/FBIOE.2017.00076","DOI":"10.3389\/FBIOE.2017.00076"},{"key":"41_CR34","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1016\/j.joca.2003.08.009","volume":"12","author":"CJ Hunter","year":"2004","unstructured":"Hunter, C.J., Mouw, J.K., Levenston, M.E.: Dynamic compression of chondrocyte-seeded fibrin gels: effects on matrix accumulation and mechanical stiffness. Osteoarthr. Cartil. 12, 117\u2013130 (2004). https:\/\/doi.org\/10.1016\/j.joca.2003.08.009","journal-title":"Osteoarthr. Cartil."},{"key":"41_CR35","doi-asserted-by":"publisher","first-page":"291","DOI":"10.1136\/ARD.2004.022400","volume":"64","author":"F Eckstein","year":"2005","unstructured":"Eckstein, F., Lemberger, B., Gratzke, C., et al.: In vivo cartilage deformation after different types of activity and its dependence on physical training status. Ann. Rheum. Dis. 64, 291\u2013295 (2005). https:\/\/doi.org\/10.1136\/ARD.2004.022400","journal-title":"Ann. Rheum. Dis."},{"key":"41_CR36","doi-asserted-by":"publisher","first-page":"2127","DOI":"10.1016\/J.SPINEE.2013.11.062","volume":"14","author":"TL Tsai","year":"2014","unstructured":"Tsai, T.L., Nelson, B.C., Anderson, P.A., et al.: Intervertebral disc and stem cells cocultured in biomimetic extracellular matrix stimulated by cyclic compression in perfusion bioreactor. Spine J. 14, 2127\u20132140 (2014). https:\/\/doi.org\/10.1016\/J.SPINEE.2013.11.062","journal-title":"Spine J."},{"key":"41_CR37","doi-asserted-by":"publisher","first-page":"1","DOI":"10.4061\/2011\/620247","volume":"2011","author":"D Brindley","year":"2011","unstructured":"Brindley, D., Moorthy, K., Lee, J.H., et al.: Bioprocess forces and their impact on cell behavior: implications for bone regeneration therapy. J. Tissue Eng. 2011, 1\u201313 (2011). https:\/\/doi.org\/10.4061\/2011\/620247","journal-title":"J. Tissue Eng."},{"key":"41_CR38","doi-asserted-by":"publisher","first-page":"719","DOI":"10.1016\/J.MEDENGPHY.2015.05.015","volume":"37","author":"N Rosa","year":"2015","unstructured":"Rosa, N., Simoes, R., Magalh\u00e3es, F.D., Marques, A.T.: From mechanical stimulus to bone formation: a review. Med. Eng. Phys. 37, 719\u2013728 (2015). https:\/\/doi.org\/10.1016\/J.MEDENGPHY.2015.05.015","journal-title":"Med. Eng. Phys."},{"key":"41_CR39","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1016\/J.JDSR.2013.10.004","volume":"50","author":"T Takano-Yamamoto","year":"2014","unstructured":"Takano-Yamamoto, T.: Osteocyte function under compressive mechanical force. Jpn. Dental Sci. Rev. 50, 29\u201339 (2014). https:\/\/doi.org\/10.1016\/J.JDSR.2013.10.004","journal-title":"Jpn. Dental Sci. Rev."},{"key":"41_CR40","doi-asserted-by":"publisher","first-page":"256","DOI":"10.3109\/17453677508989216","volume":"46","author":"LE Lanyon","year":"1975","unstructured":"Lanyon, L.E., Hampson, W.G.J., Goodship, A.E., Shah, J.S.: Bone deformation recorded in vivo from strain gauges attached to the human tibial shaft. Acta Orthop. Scand. 46, 256\u2013268 (1975). https:\/\/doi.org\/10.3109\/17453677508989216","journal-title":"Acta Orthop. Scand."},{"key":"41_CR41","doi-asserted-by":"publisher","first-page":"408","DOI":"10.1002\/JCTB.1918","volume":"83","author":"P Godara","year":"2008","unstructured":"Godara, P., McFarland, C.D., Nordon, R.E.: Design of bioreactors for mesenchymal stem cell tissue engineering. J. Chem. Technol. Biotechnol. 83, 408\u2013420 (2008). https:\/\/doi.org\/10.1002\/JCTB.1918","journal-title":"J. Chem. Technol. Biotechnol."},{"key":"41_CR42","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1089\/TEN.TEB.2010.0612","volume":"17","author":"J Rauh","year":"2011","unstructured":"Rauh, J., Milan, F., G\u00fcnther, K.P., Stiehler, M.: Bioreactor systems for bone tissue engineering. Tissue Eng. Part B Rev. 17, 263\u2013280 (2011). https:\/\/doi.org\/10.1089\/TEN.TEB.2010.0612","journal-title":"Tissue Eng. Part B Rev."},{"key":"41_CR43","doi-asserted-by":"publisher","DOI":"10.1088\/1748-6041\/11\/6\/065002","volume":"11","author":"K Song","year":"2016","unstructured":"Song, K., Li, W., Wang, H., et al.: Development and fabrication of a two-layer tissue engineered osteochondral composite using hybrid hydrogel-cancellous bone scaffolds in a spinner flask. Biomed. Mater. 11, 065002 (2016). https:\/\/doi.org\/10.1088\/1748-6041\/11\/6\/065002","journal-title":"Biomed. Mater."},{"key":"41_CR44","doi-asserted-by":"publisher","first-page":"267","DOI":"10.11344\/NANO.3.267","volume":"3","author":"M Nishi","year":"2011","unstructured":"Nishi, M., Matsumoto, R., Dong, J., Uemura, T.: Regeneration of bone tissue in a controlled in vitro environment with a rotating wall vessel bioreactor. Nano Biomed. 3, 267\u2013274 (2011). https:\/\/doi.org\/10.11344\/NANO.3.267","journal-title":"Nano Biomed."},{"key":"41_CR45","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1152\/AJPRENAL.2001.281.1.F12\/ASSET\/IMAGES\/LARGE\/H20710372005.JPEG","volume":"281","author":"TG Hammond","year":"2001","unstructured":"Hammond, T.G., Hammond, J.M.: Optimized suspension culture: the rotating-wall vessel. Am. J. Physiol. Renal Physiol. 281, 12\u201325 (2001). https:\/\/doi.org\/10.1152\/AJPRENAL.2001.281.1.F12\/ASSET\/IMAGES\/LARGE\/H20710372005.JPEG","journal-title":"Am. J. Physiol. Renal Physiol."},{"key":"41_CR46","doi-asserted-by":"publisher","first-page":"807","DOI":"10.1089\/10763270260424169","volume":"8","author":"T Davisson","year":"2004","unstructured":"Davisson, T., Sah, R.L., Ratcliffe, A.: Perfusion increases cell content and matrix synthesis in chondrocyte three-dimensional cultures. Tissue Eng. 8, 807\u2013816 (2004). https:\/\/doi.org\/10.1089\/10763270260424169","journal-title":"Tissue Eng."},{"key":"41_CR47","doi-asserted-by":"crossref","unstructured":"Mizuno, S., Allemann, F., Glowacki, J.: Effects of medium perfusion on matrix production by bovine chondrocytes in three-dimensional collagen sponges. J Biomed Mater Res. (2001). https:\/\/doi.org\/10.1002\/1097-4636(20010905)56:3<368::AID-JBM1105>3.0.CO;2-V","DOI":"10.1002\/1097-4636(20010905)56:3<368::AID-JBM1105>3.0.CO;2-V"},{"key":"41_CR48","doi-asserted-by":"publisher","DOI":"10.1371\/JOURNAL.PONE.0227553","volume":"15","author":"C Gamez","year":"2020","unstructured":"Gamez, C., Schneider-Wald, B., Schuette, A., et al.: Bioreactor for mobilization of mesenchymal stem\/stromal cells into scaffolds under mechanical stimulation: preliminary results. PLoS One. 15, e0227553 (2020). https:\/\/doi.org\/10.1371\/JOURNAL.PONE.0227553","journal-title":"PLoS One"},{"key":"41_CR49","doi-asserted-by":"publisher","first-page":"1414","DOI":"10.1016\/J.MEHY.2006.05.044","volume":"67","author":"C Zhang","year":"2006","unstructured":"Zhang, C., Zhang, X., Wu, H., et al.: Direct compression as an appropriately mechanical environment in bone tissue reconstruction in vitro. Med. Hypotheses. 67, 1414\u20131418 (2006). https:\/\/doi.org\/10.1016\/J.MEHY.2006.05.044","journal-title":"Med. Hypotheses"},{"key":"41_CR50","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1007\/978-1-4939-2938-2_16","volume":"1340","author":"K Shahin","year":"2015","unstructured":"Shahin, K., Doran, P.M.: Shear and compression bioreactor for cartilage synthesis. Methods Mol. Biol. 1340, 221\u2013233 (2015). https:\/\/doi.org\/10.1007\/978-1-4939-2938-2_16","journal-title":"Methods Mol. Biol."},{"key":"41_CR51","doi-asserted-by":"publisher","unstructured":"Chang, C.H., Lin, C.C., Chou, C.H., et al.: Novel bioreactors for osteochondral tissue engineering. 17, 38\u201343 (2012). https:\/\/doi.org\/10.4015\/S101623720500007X","DOI":"10.4015\/S101623720500007X"},{"key":"41_CR52","doi-asserted-by":"publisher","first-page":"3253","DOI":"10.1002\/BIT.27164","volume":"116","author":"J Navarro","year":"2019","unstructured":"Navarro, J., Swayambunathan, J., Janes, M.E., et al.: Dual-chambered membrane bioreactor for coculture of stratified cell populations. Biotechnol. Bioeng. 116, 3253\u20133268 (2019). https:\/\/doi.org\/10.1002\/BIT.27164","journal-title":"Biotechnol. Bioeng."}],"container-title":["Lecture Notes in Mechanical Engineering","Innovations in Mechanical Engineering IV"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-3-031-93554-1_41","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,9]],"date-time":"2025-06-09T13:44:58Z","timestamp":1749476698000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-3-031-93554-1_41"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"ISBN":["9783031935534","9783031935541"],"references-count":52,"URL":"https:\/\/doi.org\/10.1007\/978-3-031-93554-1_41","relation":{},"ISSN":["2195-4356","2195-4364"],"issn-type":[{"value":"2195-4356","type":"print"},{"value":"2195-4364","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025]]},"assertion":[{"value":"10 June 2025","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}},{"value":"icieng","order":1,"name":"conference_acronym","label":"Conference Acronym","group":{"name":"ConferenceInfo","label":"Conference Information"}},{"value":"International Conference Innovation in Engineering","order":2,"name":"conference_name","label":"Conference Name","group":{"name":"ConferenceInfo","label":"Conference Information"}},{"value":"Prague","order":3,"name":"conference_city","label":"Conference City","group":{"name":"ConferenceInfo","label":"Conference Information"}},{"value":"Czech Republic","order":4,"name":"conference_country","label":"Conference Country","group":{"name":"ConferenceInfo","label":"Conference Information"}},{"value":"2025","order":5,"name":"conference_year","label":"Conference Year","group":{"name":"ConferenceInfo","label":"Conference Information"}},{"value":"18 June 2025","order":7,"name":"conference_start_date","label":"Conference Start Date","group":{"name":"ConferenceInfo","label":"Conference Information"}},{"value":"20 June 2025","order":8,"name":"conference_end_date","label":"Conference End Date","group":{"name":"ConferenceInfo","label":"Conference Information"}},{"value":"4","order":9,"name":"conference_number","label":"Conference Number","group":{"name":"ConferenceInfo","label":"Conference Information"}},{"value":"icieng2025","order":10,"name":"conference_id","label":"Conference ID","group":{"name":"ConferenceInfo","label":"Conference Information"}},{"value":"https:\/\/icieng.eu\/","order":11,"name":"conference_url","label":"Conference URL","group":{"name":"ConferenceInfo","label":"Conference Information"}}]}}