{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T09:58:49Z","timestamp":1777456729525,"version":"3.51.4"},"reference-count":23,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[1981,8,1]],"date-time":"1981-08-01T00:00:00Z","timestamp":365472000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Pflugers Arch - Eur J Physiol"],"published-print":{"date-parts":[[1981,8]]},"DOI":"10.1007\/bf00656997","type":"journal-article","created":{"date-parts":[[2004,11,28]],"date-time":"2004-11-28T04:27:52Z","timestamp":1101616072000},"page":"85-100","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13461,"title":["Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches"],"prefix":"10.1007","volume":"391","author":[{"given":"O. P.","family":"Hamill","sequence":"first","affiliation":[]},{"given":"A.","family":"Marty","sequence":"additional","affiliation":[]},{"given":"E.","family":"Neher","sequence":"additional","affiliation":[]},{"given":"B.","family":"Sakmann","sequence":"additional","affiliation":[]},{"given":"F. J.","family":"Sigworth","sequence":"additional","affiliation":[]}],"member":"297","reference":[{"key":"BF00656997_CR1","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1113\/jphysiol.1976.sp011638","volume":"263","author":"BL Brandt","year":"1976","unstructured":"Brandt BL, Hagiwara S, Kidokoro Y, Miyazaki S (1976) Action potentials in the rat chromaffin cell and effects of Acetylcholine. J Physiol (Lond) 263:417\u2013439","journal-title":"J Physiol (Lond)"},{"key":"BF00656997_CR2","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1038\/newbio244047a0","volume":"244","author":"A Cass","year":"1973","unstructured":"Cass A, Dalmark M (1973) Equilibrium dialysis of ions in nystatintreated red cells. Nature (New Biol) 244:47\u201349","journal-title":"Nature (New Biol)"},{"key":"BF00656997_CR3","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1083\/jcb.76.1.12","volume":"76","author":"EM Fenwick","year":"1978","unstructured":"Fenwick EM, Fajdiga PB, Howe NBS, Livett BG (1978) Functional and morphological characterization of isolated bovine adrenal medullary cells. J Cell Biol 76:12\u201330","journal-title":"J Cell Biol"},{"key":"BF00656997_CR4","first-page":"509","volume":"288","author":"PW Gage","year":"1979","unstructured":"Gage PW, Van Helden D (1979) Effects of permeant monovalent cations on end-plate channels. J Physiol (Lond) 288:509\u2013528","journal-title":"J Physiol (Lond)"},{"key":"BF00656997_CR5","first-page":"41","volume":"312","author":"OP Hamill","year":"1981","unstructured":"Hamill OP, Sakmann B (1981) A cell-free method for recording single channel currents from biological membranes. J Physiol (Lond) 312:41\u201342P","journal-title":"J Physiol (Lond)"},{"key":"BF00656997_CR6","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1085\/jgp.75.3.297","volume":"75","author":"R Horn","year":"1980","unstructured":"Horn R, Brodwick MS (1980) Acetylcholine-induced current in perfused rat myoballs. J Gen Physiol 75:297\u2013321","journal-title":"J Gen Physiol"},{"key":"BF00656997_CR7","doi-asserted-by":"crossref","first-page":"6930","DOI":"10.1073\/pnas.77.11.6930","volume":"77","author":"R Horn","year":"1980","unstructured":"Horn R, Patlak JB (1980) Single channel currents from excised patches of muscle membrane. Proc Natl Acad Sci USA 77:6930\u20136934","journal-title":"Proc Natl Acad Sci USA"},{"key":"BF00656997_CR8","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1113\/jphysiol.1977.sp011968","volume":"270","author":"PG Kostyuk","year":"1977","unstructured":"Kostyuk PG, Krishtal OA (1977) Separation of sodium and calcium currents in the somatic membrane of mollusc neurones. J Physiol (Lond) 270:545\u2013568","journal-title":"J Physiol (Lond)"},{"key":"BF00656997_CR9","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1038\/257691a0","volume":"257","author":"PG Kostyuk","year":"1976","unstructured":"Kostyuk PG, Krishtal OA, Pidoplichko VI (1976) Effect of internal fluoride and phosphate on membrane currents during intracellular dialysis of nerve cells. Nature 257:691\u2013693","journal-title":"Nature"},{"key":"BF00656997_CR10","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1007\/BF01063316","volume":"7","author":"OA Krishtal","year":"1975","unstructured":"Krishtal OA, Pidoplichko VI (1975) Intracellular perfusion of Helix neurons. Neurophysiol (Kiev) 7:258\u2013259","journal-title":"Neurophysiol (Kiev)"},{"key":"BF00656997_CR11","doi-asserted-by":"crossref","first-page":"2325","DOI":"10.1016\/0306-4522(80)90149-9","volume":"5","author":"OA Krishtal","year":"1980","unstructured":"Krishtal OA, Pidoplichko VI (1980) A receptor for protons in the nerve cell membrane. Neuroscience 5:2325\u20132327","journal-title":"Neuroscience"},{"key":"BF00656997_CR12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0005-2736(75)90053-X","volume":"413","author":"P L\u00e4uger","year":"1975","unstructured":"L\u00e4uger P (1975) Shot noise in ion channels. Biochim Biophys Acta 413:1\u201310","journal-title":"Biochim Biophys Acta"},{"key":"BF00656997_CR13","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1126\/science.87.2266.493","volume":"87","author":"I Langmuir","year":"1938","unstructured":"Langmuir I (1938) Overturning and anchoring of monolayers. Science 87:493\u2013500","journal-title":"Science"},{"key":"BF00656997_CR14","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1085\/jgp.71.5.489","volume":"71","author":"KS Lee","year":"1978","unstructured":"Lee KS, Akaike N, Brown AM (1978) Properties of internally perfused, voltage clamped, isolated nerve cell bodies. J Gen Physiol 71:489\u2013508","journal-title":"J Gen Physiol"},{"key":"BF00656997_CR15","volume-title":"Techniques in cellular physiology","author":"E Neher","year":"1981","unstructured":"Neher E (1981) Unit conductance studies in biological membranes. In: Baker PF (ed), Techniques in cellular physiology, Elsevier\/North-Holland, Amsterdam"},{"key":"BF00656997_CR16","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1038\/260799a0","volume":"260","author":"E Neher","year":"1976","unstructured":"Neher E, Sakmann B (1976) Single channel currents recorded from membrane of denervated frog muscle fibres. Nature 260:799\u2013802","journal-title":"Nature"},{"key":"BF00656997_CR17","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1007\/BF00584247","volume":"375","author":"E Neher","year":"1978","unstructured":"Neher E, Sakmann B, Steinbach JH (1978) The extracellular patch clamp: A method for resolving currents through individual open channels in biological membranes. Pfl\u00fcgers Arch 375:219\u2013228","journal-title":"Pfl\u00fcgers Arch"},{"key":"BF00656997_CR18","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/0021-9797(78)90091-7","volume":"65","author":"S Nir","year":"1978","unstructured":"Nir S, Bentz J (1978) On the forces between phospholipid bilayers. J Colloid Interface Sci 65:399\u2013412","journal-title":"J Colloid Interface Sci"},{"key":"BF00656997_CR19","doi-asserted-by":"crossref","first-page":"2750","DOI":"10.1073\/pnas.76.6.2750","volume":"76","author":"VA Parsegian","year":"1979","unstructured":"Parsegian VA, Fuller N, Rand RP (1979) Measured work of deformation and repulsion of lecithin bilayers. Proc Natl Acad Sci USA 76: 2750\u20132754","journal-title":"Proc Natl Acad Sci USA"},{"key":"BF00656997_CR20","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/0021-9797(80)90123-X","volume":"73","author":"JG Petrov","year":"1980","unstructured":"Petrov JG, Kuhn H, M\u00f6bius D (1980) Three-Phase Contact Line Motion in the deposition of spread monolayers. J Colloid Interface Sci 73:66\u201375","journal-title":"J Colloid Interface Sci"},{"key":"BF00656997_CR21","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1038\/286071a0","volume":"286","author":"B Sakmann","year":"1980","unstructured":"Sakmann B, Patlak J, Neher E (1980) Single acetylcholine-activated channels show burst-kinetics in presence of desensitizing concentrations of agonist. Nature 286:71\u201373","journal-title":"Nature"},{"key":"BF00656997_CR22","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1038\/287447a0","volume":"287","author":"FJ Sigworth","year":"1980","unstructured":"Sigworth FJ, Neher E (1980) Single Na+ channel currents observed in cultured rat muscle cells. Nature 287:447\u2013449","journal-title":"Nature"},{"key":"BF00656997_CR23","doi-asserted-by":"crossref","first-page":"1028","DOI":"10.1016\/S0006-3495(72)86141-1","volume":"12","author":"CF Stevens","year":"1972","unstructured":"Stevens CF (1972) Inferences about membrane properties from electrical noise measurements. Biophys J 12:1028\u20131047","journal-title":"Biophys J"}],"container-title":["Pfl\u00fcgers Archiv - European Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/BF00656997.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/BF00656997\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/BF00656997","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,5]],"date-time":"2019-04-05T06:39:50Z","timestamp":1554446390000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/BF00656997"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1981,8]]},"references-count":23,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1981,8]]}},"alternative-id":["BF00656997"],"URL":"https:\/\/doi.org\/10.1007\/bf00656997","relation":{},"ISSN":["0031-6768","1432-2013"],"issn-type":[{"value":"0031-6768","type":"print"},{"value":"1432-2013","type":"electronic"}],"subject":[],"published":{"date-parts":[[1981,8]]}}}