{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T16:16:29Z","timestamp":1770567389654,"version":"3.49.0"},"reference-count":96,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"funder":[{"name":"Programa Operacional Regional do Norte","award":["NORTE-01-0145-FEDER-072678"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-072678"]}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["EXPL\/SAU-PUB\/1073\/2021"],"award-info":[{"award-number":["EXPL\/SAU-PUB\/1073\/2021"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Cell. Mol. Life Sci."],"published-print":{"date-parts":[[2022,3]]},"DOI":"10.1007\/s00018-022-04172-x","type":"journal-article","created":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T05:57:45Z","timestamp":1646114265000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["MCL1 alternative polyadenylation is essential for cell survival and mitochondria morphology"],"prefix":"10.1007","volume":"79","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4149-4310","authenticated-orcid":false,"given":"Isabel","family":"Pereira-Castro","sequence":"first","affiliation":[]},{"given":"Beatriz C.","family":"Garcia","sequence":"additional","affiliation":[]},{"given":"Ana","family":"Curinha","sequence":"additional","affiliation":[]},{"given":"Ana","family":"Neves-Costa","sequence":"additional","affiliation":[]},{"given":"Eduardo","family":"Conde-Sousa","sequence":"additional","affiliation":[]},{"given":"Lu\u00eds F.","family":"Moita","sequence":"additional","affiliation":[]},{"given":"Alexandra","family":"Moreira","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2022,3,1]]},"reference":[{"issue":"6","key":"4172_CR1","doi-asserted-by":"crossref","first-page":"1173","DOI":"10.1101\/gr.132563.111","volume":"22","author":"A Derti","year":"2012","unstructured":"Derti A et al (2012) A quantitative atlas of polyadenylation in five mammals. Genome Res 22(6):1173\u20131183","journal-title":"Genome Res"},{"issue":"10","key":"4172_CR2","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1038\/s41576-019-0145-z","volume":"20","author":"AJ Gruber","year":"2019","unstructured":"Gruber AJ, Zavolan M (2019) Alternative cleavage and polyadenylation in health and disease. Nat Rev Genet 20(10):599\u2013614","journal-title":"Nat Rev Genet"},{"issue":"2","key":"4172_CR3","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1038\/nmeth.2288","volume":"10","author":"M Hoque","year":"2013","unstructured":"Hoque M et al (2013) Analysis of alternative cleavage and polyadenylation by 3\u2032 region extraction and deep sequencing. Nat Methods 10(2):133\u2013139","journal-title":"Nat Methods"},{"issue":"7556","key":"4172_CR4","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1038\/nature14321","volume":"522","author":"BD Berkovits","year":"2015","unstructured":"Berkovits BD, Mayr C (2015) Alternative 3\u2032 UTRs act as scaffolds to regulate membrane protein localization. Nature 522(7556):363\u2013367","journal-title":"Nature"},{"issue":"6","key":"4172_CR5","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1016\/j.molcel.2011.08.017","volume":"43","author":"G Di","year":"2011","unstructured":"Di G, Nishida K, Manley JL (2011) Mechanisms and consequences of alternative polyadenylation. Mol Cell 43(6):853\u2013866","journal-title":"Mol Cell"},{"issue":"1","key":"4172_CR6","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1002\/wrna.47","volume":"2","author":"CS Lutz","year":"2011","unstructured":"Lutz CS, Moreira A (2011) Alternative mRNA polyadenylation in eukaryotes: an effective regulator of gene expression. Wiley Interdiscip Rev RNA 2(1):22\u201331","journal-title":"Wiley Interdiscip Rev RNA"},{"issue":"3","key":"4172_CR7","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.tcb.2015.10.012","volume":"26","author":"C Mayr","year":"2016","unstructured":"Mayr C (2016) Evolution and biological roles of alternative 3\u2032UTRs. Trends Cell Biol 26(3):227\u2013237","journal-title":"Trends Cell Biol"},{"issue":"1","key":"4172_CR8","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1038\/nrm.2016.116","volume":"18","author":"B Tian","year":"2017","unstructured":"Tian B, Manley JL (2017) Alternative polyadenylation of mRNA precursors. Nat Rev Mol Cell Biol 18(1):18\u201330","journal-title":"Nat Rev Mol Cell Biol"},{"issue":"17","key":"4172_CR9","doi-asserted-by":"crossref","first-page":"7028","DOI":"10.1073\/pnas.0900028106","volume":"106","author":"Z Ji","year":"2009","unstructured":"Ji Z et al (2009) Progressive lengthening of 3\u2032 untranslated regions of mRNAs by alternative polyadenylation during mouse embryonic development. Proc Natl Acad Sci USA 106(17):7028\u20137033","journal-title":"Proc Natl Acad Sci USA"},{"issue":"5","key":"4172_CR10","doi-asserted-by":"crossref","first-page":"812","DOI":"10.1101\/gr.146886.112","volume":"23","author":"P Miura","year":"2013","unstructured":"Miura P et al (2013) Widespread and extensive lengthening of 3\u2032 UTRs in the mammalian brain. Genome Res 23(5):812\u2013825","journal-title":"Genome Res"},{"issue":"7","key":"4172_CR11","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1101\/gr.10.7.1001","volume":"10","author":"E Beaudoing","year":"2000","unstructured":"Beaudoing E et al (2000) Patterns of variant polyadenylation signal usage in human genes. Genome Res 10(7):1001\u20131010","journal-title":"Genome Res"},{"issue":"5574","key":"4172_CR12","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1038\/263211a0","volume":"263","author":"NJ Proudfoot","year":"1976","unstructured":"Proudfoot NJ, Brownlee GG (1976) 3\u2032 non-coding region sequences in eukaryotic messenger RNA. Nature 263(5574):211\u2013214","journal-title":"Nature"},{"issue":"11","key":"4172_CR13","doi-asserted-by":"crossref","first-page":"2658","DOI":"10.1038\/sj.emboj.7601699","volume":"26","author":"S Danckwardt","year":"2007","unstructured":"Danckwardt S et al (2007) Splicing factors stimulate polyadenylation via USEs at non-canonical 3\u2032 end formation signals. EMBO J 26(11):2658\u20132669","journal-title":"EMBO J"},{"issue":"16","key":"4172_CR14","doi-asserted-by":"crossref","first-page":"2522","DOI":"10.1101\/gad.12.16.2522","volume":"12","author":"A Moreira","year":"1998","unstructured":"Moreira A et al (1998) The upstream sequence element of the C2 complement poly(A) signal activates mRNA 3\u2032 end formation by two distinct mechanisms. Genes Dev 12(16):2522\u20132534","journal-title":"Genes Dev"},{"issue":"45","key":"4172_CR15","doi-asserted-by":"crossref","first-page":"42733","DOI":"10.1074\/jbc.M208070200","volume":"277","author":"BJ Natalizio","year":"2002","unstructured":"Natalizio BJ et al (2002) Upstream elements present in the 3\u2032-untranslated region of collagen genes influence the processing efficiency of overlapping polyadenylation signals. J Biol Chem 277(45):42733\u201342740","journal-title":"J Biol Chem"},{"issue":"9","key":"4172_CR16","doi-asserted-by":"crossref","first-page":"1523","DOI":"10.1038\/emboj.2010.42","volume":"29","author":"NM Nunes","year":"2010","unstructured":"Nunes NM et al (2010) A functional human Poly(A) site requires only a potent DSE and an A-rich upstream sequence. EMBO J 29(9):1523\u20131536","journal-title":"EMBO J"},{"issue":"4","key":"4172_CR17","volume":"11","author":"W Li","year":"2015","unstructured":"Li W et al (2015) Systematic profiling of poly(A)+ transcripts modulated by core 3\u2032 end processing and splicing factors reveals regulatory rules of alternative cleavage and polyadenylation. PLoS Genet 11(4):e1005166","journal-title":"PLoS Genet"},{"issue":"3","key":"4172_CR18","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/j.molcel.2008.12.028","volume":"33","author":"Y Shi","year":"2009","unstructured":"Shi Y et al (2009) Molecular architecture of the human pre-mRNA 3\u2032 processing complex. Mol Cell 33(3):365\u2013376","journal-title":"Mol Cell"},{"issue":"18","key":"4172_CR19","doi-asserted-by":"crossref","first-page":"9089","DOI":"10.1093\/nar\/gks655","volume":"40","author":"E de Klerk","year":"2012","unstructured":"de Klerk E et al (2012) Poly(A) binding protein nuclear 1 levels affect alternative polyadenylation. Nucleic Acids Res 40(18):9089\u20139101","journal-title":"Nucleic Acids Res"},{"issue":"21","key":"4172_CR20","doi-asserted-by":"crossref","first-page":"6264","DOI":"10.1093\/nar\/gkl794","volume":"34","author":"T Kubo","year":"2006","unstructured":"Kubo T et al (2006) Knock-down of 25 kDa subunit of cleavage factor Im in Hela cells alters alternative polyadenylation within 3\u2032-UTRs. Nucleic Acids Res 34(21):6264\u20136271","journal-title":"Nucleic Acids Res"},{"issue":"8","key":"4172_CR21","doi-asserted-by":"crossref","first-page":"878","DOI":"10.1002\/embj.201386537","volume":"33","author":"B Lackford","year":"2014","unstructured":"Lackford B et al (2014) Fip1 regulates mRNA alternative polyadenylation to promote stem cell self-renewal. EMBO J 33(8):878\u2013889","journal-title":"EMBO J"},{"issue":"5","key":"4172_CR22","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1016\/S0092-8674(00)82000-0","volume":"87","author":"Y Takagaki","year":"1996","unstructured":"Takagaki Y et al (1996) The polyadenylation factor CstF-64 regulates alternative processing of IgM heavy chain pre-mRNA during B cell differentiation. Cell 87(5):941\u2013952","journal-title":"Cell"},{"issue":"6","key":"4172_CR23","doi-asserted-by":"crossref","first-page":"508","DOI":"10.4161\/nucl.36360","volume":"5","author":"A Curinha","year":"2014","unstructured":"Curinha A et al (2014) Implications of polyadenylation in health and disease. Nucleus 5(6):508\u2013519","journal-title":"Nucleus"},{"key":"4172_CR24","doi-asserted-by":"crossref","unstructured":"Pereira-Castro I, Moreira A (2021) On the function and relevance of alternative 3\u2032-UTRs in gene expression regulation. Wiley Interdiscip Rev RNA e1653","DOI":"10.1002\/wrna.1653"},{"issue":"5883","key":"4172_CR25","doi-asserted-by":"crossref","first-page":"1643","DOI":"10.1126\/science.1155390","volume":"320","author":"R Sandberg","year":"2008","unstructured":"Sandberg R et al (2008) Proliferating cells express mRNAs with shortened 3\u2032 untranslated regions and fewer microRNA target sites. Science 320(5883):1643\u20131647","journal-title":"Science"},{"issue":"5","key":"4172_CR26","doi-asserted-by":"crossref","first-page":"722","DOI":"10.1016\/j.stem.2020.03.003","volume":"26","author":"P Sommerkamp","year":"2020","unstructured":"Sommerkamp P et al (2020) Differential Alternative Polyadenylation Landscapes Mediate Hematopoietic Stem Cell Activation and Regulate Glutamine Metabolism. Cell Stem Cell 26(5):722-738e7","journal-title":"Cell Stem Cell"},{"issue":"9","key":"4172_CR27","volume":"12","author":"AA Pai","year":"2016","unstructured":"Pai AA et al (2016) Widespread Shortening of 3\u2032 Untranslated Regions and Increased Exon Inclusion Are Evolutionarily Conserved Features of Innate Immune Responses to Infection. PLoS Genet 12(9):e1006338","journal-title":"PLoS Genet"},{"issue":"4","key":"4172_CR28","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1016\/j.cell.2009.06.016","volume":"138","author":"C Mayr","year":"2009","unstructured":"Mayr C, Bartel DP (2009) Widespread shortening of 3\u2032UTRs by alternative cleavage and polyadenylation activates oncogenes in cancer cells. Cell 138(4):673\u2013684","journal-title":"Cell"},{"issue":"19","key":"4172_CR29","doi-asserted-by":"crossref","first-page":"5256","DOI":"10.1158\/1078-0432.CCR-12-0543","volume":"18","author":"AR Morris","year":"2012","unstructured":"Morris AR et al (2012) Alternative cleavage and polyadenylation during colorectal cancer development. Clin Cancer Res 18(19):5256\u20135266","journal-title":"Clin Cancer Res"},{"issue":"1","key":"4172_CR30","doi-asserted-by":"crossref","first-page":"3182","DOI":"10.1038\/s41467-020-16959-2","volume":"11","author":"LC Cheng","year":"2020","unstructured":"Cheng LC et al (2020) Widespread transcript shortening through alternative polyadenylation in secretory cell differentiation. Nat Commun 11(1):3182","journal-title":"Nat Commun"},{"key":"4172_CR31","unstructured":"Chen M et al. (2008) 3\u2032 UTR lengthening as a novel mechanism in regulating cellular senescence. Genome Res"},{"issue":"38","key":"4172_CR32","doi-asserted-by":"crossref","first-page":"15864","DOI":"10.1073\/pnas.1112672108","volume":"108","author":"V Hilgers","year":"2011","unstructured":"Hilgers V et al (2011) Neural-specific elongation of 3\u2032 UTRs during Drosophila development. Proc Natl Acad Sci U S A 108(38):15864\u201315869","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"6","key":"4172_CR33","doi-asserted-by":"crossref","first-page":"1490","DOI":"10.1002\/eji.201545663","volume":"46","author":"RG Domingues","year":"2016","unstructured":"Domingues RG et al (2016) CD5 expression is regulated during human T-cell activation by alternative polyadenylation, PTBP1, and miR-204. Eur J Immunol 46(6):1490\u20131503","journal-title":"Eur J Immunol"},{"issue":"6","key":"4172_CR34","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1016\/j.bbagrm.2017.03.002","volume":"1860","author":"SO Braz","year":"2017","unstructured":"Braz SO et al (2017) Expression of Rac1 alternative 3\u2032 UTRs is a cell specific mechanism with a function in dendrite outgrowth in cortical neurons. Biochim Biophys Acta Gene Regul Mech 1860(6):685\u2013694","journal-title":"Biochim Biophys Acta Gene Regul Mech"},{"issue":"12","key":"4172_CR35","doi-asserted-by":"crossref","first-page":"2431","DOI":"10.1038\/emboj.2011.156","volume":"30","author":"PA Pinto","year":"2011","unstructured":"Pinto PA et al (2011) RNA polymerase II kinetics in polo polyadenylation signal selection. EMBO J 30(12):2431\u20132444","journal-title":"EMBO J"},{"issue":"6466","key":"4172_CR36","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1126\/science.aax1694","volume":"366","author":"A de Morree","year":"2019","unstructured":"de Morree A et al (2019) Alternative polyadenylation of Pax3 controls muscle stem cell fate and muscle function. Science 366(6466):734\u2013738","journal-title":"Science"},{"issue":"1","key":"4172_CR37","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.cell.2008.05.045","volume":"134","author":"JJ An","year":"2008","unstructured":"An JJ et al (2008) Distinct role of long 3\u2032 UTR BDNF mRNA in spine morphology and synaptic plasticity in hippocampal neurons. Cell 134(1):175\u2013187","journal-title":"Cell"},{"issue":"1","key":"4172_CR38","doi-asserted-by":"crossref","first-page":"521","DOI":"10.4049\/jimmunol.181.1.521","volume":"181","author":"I Dzhagalov","year":"2008","unstructured":"Dzhagalov I, Dunkle A, He YW (2008) The anti-apoptotic Bcl-2 family member Mcl-1 promotes T lymphocyte survival at multiple stages. J Immunol 181(1):521\u2013528","journal-title":"J Immunol"},{"issue":"8","key":"4172_CR39","doi-asserted-by":"crossref","first-page":"3516","DOI":"10.1073\/pnas.90.8.3516","volume":"90","author":"KM Kozopas","year":"1993","unstructured":"Kozopas KM et al (1993) MCL1, a gene expressed in programmed myeloid cell differentiation, has sequence similarity to BCL2. Proc Natl Acad Sci 90(8):3516\u20133520","journal-title":"Proc Natl Acad Sci"},{"issue":"2","key":"4172_CR40","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/j.biocel.2004.04.007","volume":"37","author":"J Michels","year":"2005","unstructured":"Michels J, Johnson PW, Packham G (2005) Mcl-1. Int J Biochem Cell Biol 37(2):267\u2013271","journal-title":"Int J Biochem Cell Biol"},{"issue":"7","key":"4172_CR41","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1016\/j.tcb.2019.03.004","volume":"29","author":"VV Senichkin","year":"2019","unstructured":"Senichkin VV et al (2019) Molecular comprehension of Mcl-1: from gene structure to cancer therapy. Trends Cell Biol 29(7):549\u2013562","journal-title":"Trends Cell Biol"},{"issue":"6967","key":"4172_CR42","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nature02067","volume":"426","author":"JT Opferman","year":"2003","unstructured":"Opferman JT et al (2003) Development and maintenance of B and T lymphocytes requires antiapoptotic MCL-1. Nature 426(6967):671\u2013676","journal-title":"Nature"},{"issue":"11","key":"4172_CR43","doi-asserted-by":"crossref","first-page":"2152","DOI":"10.1038\/leu.2016.213","volume":"30","author":"Y Fernandez-Marrero","year":"2016","unstructured":"Fernandez-Marrero Y et al (2016) Survival control of malignant lymphocytes by anti-apoptotic MCL-1. Leukemia 30(11):2152\u20132159","journal-title":"Leukemia"},{"issue":"2","key":"4172_CR44","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1038\/s41419-017-0035-2","volume":"9","author":"KJ Campbell","year":"2018","unstructured":"Campbell KJ et al (2018) MCL-1 is a prognostic indicator and drug target in breast cancer. Cell Death Dis 9(2):19","journal-title":"Cell Death Dis"},{"issue":"3","key":"4172_CR45","doi-asserted-by":"crossref","first-page":"267","DOI":"10.4161\/cbt.4.3.1496","volume":"4","author":"L Song","year":"2005","unstructured":"Song L et al (2005) Mcl-1 regulates survival and sensitivity to diverse apoptotic stimuli in human non-small cell lung cancer cells. Cancer Biol Ther 4(3):267\u2013276","journal-title":"Cancer Biol Ther"},{"issue":"1","key":"4172_CR46","first-page":"25","volume":"28","author":"B Fleischer","year":"2006","unstructured":"Fleischer B et al (2006) Mcl-1 is an anti-apoptotic factor for human hepatocellular carcinoma. Int J Oncol 28(1):25\u201332","journal-title":"Int J Oncol"},{"issue":"12","key":"4172_CR47","doi-asserted-by":"crossref","first-page":"1647","DOI":"10.1093\/bioinformatics\/bts199","volume":"28","author":"M Kearse","year":"2012","unstructured":"Kearse M et al (2012) Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28(12):1647\u20131649","journal-title":"Bioinformatics"},{"issue":"9","key":"4172_CR48","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/29.9.e45","volume":"29","author":"MW Pfaffl","year":"2001","unstructured":"Pfaffl MW (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29(9):e45","journal-title":"Nucleic Acids Res"},{"key":"4172_CR49","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1186\/1472-6750-8-91","volume":"8","author":"H Liu","year":"2008","unstructured":"Liu H, Naismith JH (2008) An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol. BMC Biotechnol 8:91","journal-title":"BMC Biotechnol"},{"issue":"17","key":"4172_CR50","doi-asserted-by":"crossref","first-page":"7016","DOI":"10.1073\/pnas.0811523106","volume":"106","author":"HW Hwang","year":"2009","unstructured":"Hwang HW, Wentzel EA, Mendell JT (2009) Cell-cell contact globally activates microRNA biogenesis. Proc Natl Acad Sci U S A 106(17):7016\u20137021","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"8","key":"4172_CR51","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1038\/nmeth.3047","volume":"11","author":"NE Sanjana","year":"2014","unstructured":"Sanjana NE, Shalem O, Zhang F (2014) Improved vectors and genome-wide libraries for CRISPR screening. Nat Methods 11(8):783\u2013784","journal-title":"Nat Methods"},{"issue":"7","key":"4172_CR52","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","volume":"9","author":"J Schindelin","year":"2012","unstructured":"Schindelin J et al (2012) Fiji: an open-source platform for biological-image analysis. Nat Methods 9(7):676\u2013682","journal-title":"Nat Methods"},{"issue":"1","key":"4172_CR53","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1186\/s12859-017-1934-z","volume":"18","author":"CT Rueden","year":"2017","unstructured":"Rueden CT et al (2017) Image J2: ImageJ for the next generation of scientific image data. BMC Bioinformat 18(1):529","journal-title":"BMC Bioinformat"},{"issue":"12","key":"4172_CR54","doi-asserted-by":"crossref","first-page":"1226","DOI":"10.1038\/s41592-019-0582-9","volume":"16","author":"S Berg","year":"2019","unstructured":"Berg S et al (2019) ilastik: interactive machine learning for (bio)image analysis. Nat Methods 16(12):1226\u20131232","journal-title":"Nat Methods"},{"key":"4172_CR55","unstructured":"Vorkel D, Haase R (2020) GPU-accelerating ImageJ Macro image processing workflows using CLIJ. arXiv preprint arXiv:2008.11799"},{"key":"4172_CR56","doi-asserted-by":"publisher","DOI":"10.1101\/2020.11.19.386565","author":"R Haase","year":"2020","unstructured":"Haase R et al (2020) Interactive design of GPU-accelerated Image Data Flow Graphs and cross-platform deployment using multi-lingual code generation. bioRxiv. https:\/\/doi.org\/10.1101\/2020.11.19.386565","journal-title":"bioRxiv"},{"issue":"1","key":"4172_CR57","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1038\/s41592-019-0650-1","volume":"17","author":"R Haase","year":"2020","unstructured":"Haase R et al (2020) CLIJ: GPU-accelerated image processing for everyone. Nat Methods 17(1):5\u20136","journal-title":"Nat Methods"},{"issue":"8","key":"4172_CR58","doi-asserted-by":"crossref","first-page":"832","DOI":"10.1109\/34.709601","volume":"20","author":"TK Ho","year":"1998","unstructured":"Ho TK (1998) The random subspace method for constructing decision forests. IEEE Trans Pattern Anal Mach Intell 20(8):832\u2013844","journal-title":"IEEE Trans Pattern Anal Mach Intell"},{"key":"4172_CR59","doi-asserted-by":"crossref","unstructured":"Schmidt U et al (2018) Cell detection with star-convex polygons. In international conference on medical image computing and computer-assisted intervention. Springer","DOI":"10.1007\/978-3-030-00934-2_30"},{"issue":"14","key":"4172_CR60","doi-asserted-by":"crossref","first-page":"1840","DOI":"10.1093\/bioinformatics\/btt276","volume":"29","author":"J Ollion","year":"2013","unstructured":"Ollion J et al (2013) TANGO: a generic tool for high-throughput 3D image analysis for studying nuclear organization. Bioinformatics 29(14):1840\u20131841","journal-title":"Bioinformatics"},{"issue":"1","key":"4172_CR61","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1038\/s41592-020-01018-x","volume":"18","author":"C Stringer","year":"2021","unstructured":"Stringer C et al (2021) Cellpose: a generalist algorithm for cellular segmentation. Nat Methods 18(1):100\u2013106","journal-title":"Nat Methods"},{"issue":"22","key":"4172_CR62","doi-asserted-by":"crossref","first-page":"3532","DOI":"10.1093\/bioinformatics\/btw413","volume":"32","author":"D Legland","year":"2016","unstructured":"Legland D, Arganda-Carreras I, Andrey P (2016) MorphoLibJ: integrated library and plugins for mathematical morphology with ImageJ. Bioinformatics 32(22):3532\u20133534","journal-title":"Bioinformatics"},{"issue":"29","key":"4172_CR63","doi-asserted-by":"crossref","first-page":"22136","DOI":"10.1074\/jbc.M909572199","volume":"275","author":"CD Bingle","year":"2000","unstructured":"Bingle CD et al (2000) Exon skipping in Mcl-1 results in a bcl-2 homology domain 3 only gene product that promotes cell death. J Biol Chem 275(29):22136\u201322146","journal-title":"J Biol Chem"},{"issue":"33","key":"4172_CR64","doi-asserted-by":"crossref","first-page":"25255","DOI":"10.1074\/jbc.M909826199","volume":"275","author":"J Bae","year":"2000","unstructured":"Bae J et al (2000) MCL-1S, a splicing variant of the antiapoptotic BCL-2 family member MCL-1, encodes a proapoptotic protein possessing only the BH3 domain. J Biol Chem 275(33):25255\u201325261","journal-title":"J Biol Chem"},{"key":"4172_CR65","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1186\/s12865-015-0113-0","volume":"16","author":"CP Corkum","year":"2015","unstructured":"Corkum CP et al (2015) Immune cell subsets and their gene expression profiles from human PBMC isolated by Vacutainer Cell Preparation Tube (CPT) and standard density gradient. BMC Immunol 16:48","journal-title":"BMC Immunol"},{"key":"4172_CR66","doi-asserted-by":"crossref","first-page":"5465","DOI":"10.1038\/ncomms6465","volume":"5","author":"AR Gruber","year":"2014","unstructured":"Gruber AR et al (2014) Global 3\u2032 UTR shortening has a limited effect on protein abundance in proliferating T cells. Nat Commun 5:5465","journal-title":"Nat Commun"},{"issue":"6","key":"4172_CR67","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1038\/ncb2488","volume":"14","author":"RM Perciavalle","year":"2012","unstructured":"Perciavalle RM et al (2012) Anti-apoptotic MCL-1 localizes to the mitochondrial matrix and couples mitochondrial fusion to respiration. Nat Cell Biol 14(6):575\u2013583","journal-title":"Nat Cell Biol"},{"issue":"44","key":"4172_CR68","doi-asserted-by":"crossref","first-page":"32233","DOI":"10.1074\/jbc.M706408200","volume":"282","author":"M Germain","year":"2007","unstructured":"Germain M, Duronio V (2007) The N terminus of the anti-apoptotic BCL-2 homologue MCL-1 regulates its localization and function. J Biol Chem 282(44):32233\u201332242","journal-title":"J Biol Chem"},{"issue":"Pt 3","key":"4172_CR69","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1042\/BJ20041596","volume":"387","author":"S Jamil","year":"2005","unstructured":"Jamil S et al (2005) A proteolytic fragment of Mcl-1 exhibits nuclear localization and regulates cell growth by interaction with Cdk1. Biochem J 387(Pt 3):659\u2013667","journal-title":"Biochem J"},{"issue":"11","key":"4172_CR70","doi-asserted-by":"crossref","first-page":"1739","DOI":"10.1038\/cdd.2010.56","volume":"17","author":"P Pawlikowska","year":"2010","unstructured":"Pawlikowska P et al (2010) ATM-dependent expression of IEX-1 controls nuclear accumulation of Mcl-1 and the DNA damage response. Cell Death Differ 17(11):1739\u20131750","journal-title":"Cell Death Differ"},{"issue":"1","key":"4172_CR71","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1101\/gr.193995.115","volume":"26","author":"J Neve","year":"2016","unstructured":"Neve J et al (2016) Subcellular RNA profiling links splicing and nuclear DICER1 to alternative cleavage and polyadenylation. Genome Res 26(1):24\u201335","journal-title":"Genome Res"},{"key":"4172_CR72","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.canlet.2018.12.020","volume":"446","author":"V Labi","year":"2019","unstructured":"Labi V, Schoeler K, Melamed D (2019) miR-17 ~ 92 in lymphocyte development and lymphomagenesis. Cancer Lett 446:73\u201380","journal-title":"Cancer Lett"},{"issue":"7","key":"4172_CR73","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1038\/nrg1379","volume":"5","author":"L He","year":"2004","unstructured":"He L, Hannon GJ (2004) MicroRNAs: small RNAs with a big role in gene regulation. Nat Rev Genet 5(7):522\u2013531","journal-title":"Nat Rev Genet"},{"issue":"15","key":"4172_CR74","doi-asserted-by":"crossref","first-page":"e00581","DOI":"10.1128\/MCB.00581-18","volume":"39","author":"MS Oliveira","year":"2019","unstructured":"Oliveira MS et al (2019) Cell Cycle Kinase Polo Is Controlled by a Widespread 3\u2032 Untranslated Region Regulatory Sequence in Drosophila melanogaster. Mol Cell Biol 39(15):e00581\u201318","journal-title":"Mol Cell Biol"},{"issue":"17","key":"4172_CR75","doi-asserted-by":"crossref","first-page":"1770","DOI":"10.1101\/gad.17268411","volume":"25","author":"NJ Proudfoot","year":"2011","unstructured":"Proudfoot NJ (2011) Ending the message: poly(A) signals then and now. Genes Dev 25(17):1770\u20131782","journal-title":"Genes Dev"},{"issue":"10","key":"4172_CR76","doi-asserted-by":"crossref","DOI":"10.1038\/cddis.2017.529","volume":"8","author":"Y Zhao","year":"2017","unstructured":"Zhao Y et al (2017) Demethylzeylasteral inhibits cell proliferation and induces apoptosis through suppressing MCL1 in melanoma cells. Cell Death Dis 8(10):e3133","journal-title":"Cell Death Dis"},{"issue":"2","key":"4172_CR77","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1038\/s41419-020-2351-1","volume":"11","author":"RH Whitaker","year":"2020","unstructured":"Whitaker RH, Placzek WJ (2020) MCL1 binding to the reverse BH3 motif of P18INK4C couples cell survival to cell proliferation. Cell Death Dis 11(2):156","journal-title":"Cell Death Dis"},{"issue":"1","key":"4172_CR78","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.1038\/s42003-021-02564-6","volume":"4","author":"H Widden","year":"2021","unstructured":"Widden H, Placzek WJ (2021) The multiple mechanisms of MCL1 in the regulation of cell fate. Commun Biol 4(1):1029","journal-title":"Commun Biol"},{"issue":"1","key":"4172_CR79","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1091\/mbc.E15-01-0028","volume":"27","author":"G Morciano","year":"2016","unstructured":"Morciano G et al (2016) Mcl-1 involvement in mitochondrial dynamics is associated with apoptotic cell death. Mol Biol Cell 27(1):20\u201334","journal-title":"Mol Biol Cell"},{"issue":"10","key":"4172_CR80","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1111\/boc.201600019","volume":"108","author":"G Morciano","year":"2016","unstructured":"Morciano G et al (2016) Intersection of mitochondrial fission and fusion machinery with apoptotic pathways: Role of Mcl-1. Biol Cell 108(10):279\u2013293","journal-title":"Biol Cell"},{"issue":"3","key":"4172_CR81","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1016\/j.stemcr.2018.01.005","volume":"10","author":"ML Rasmussen","year":"2018","unstructured":"Rasmussen ML et al (2018) A Non-apoptotic function of MCL-1 in promoting pluripotency and modulating mitochondrial dynamics in stem cells. Stem Cell Reports 10(3):684\u2013692","journal-title":"Stem Cell Reports"},{"issue":"4","key":"4172_CR82","doi-asserted-by":"crossref","first-page":"101015","DOI":"10.1016\/j.isci.2020.101015","volume":"23","author":"ML Rasmussen","year":"2020","unstructured":"Rasmussen ML et al (2020) MCL-1 inhibition by selective BH3 mimetics disrupts mitochondrial dynamics causing loss of viability and functionality of human cardiomyocytes. IScience 23(4):101015","journal-title":"IScience"},{"issue":"2","key":"4172_CR83","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1016\/j.bbamcr.2014.11.002","volume":"1853","author":"AP Leonard","year":"2015","unstructured":"Leonard AP et al (2015) Quantitative analysis of mitochondrial morphology and membrane potential in living cells using high-content imaging, machine learning, and morphological binning. Biochim Biophys Acta 1853(2):348\u2013360","journal-title":"Biochim Biophys Acta"},{"issue":"2","key":"4172_CR84","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1083\/jcb.200211046","volume":"160","author":"H Chen","year":"2003","unstructured":"Chen H et al (2003) Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion and are essential for embryonic development. J Cell Biol 160(2):189\u2013200","journal-title":"J Cell Biol"},{"key":"4172_CR85","doi-asserted-by":"publisher","unstructured":"Cohen MM, Tareste D (2018) Recent insights into the structure and function of Mitofusins in mitochondrial fusion. F10000Res https:\/\/doi.org\/10.12688\/f1000research.16629.1","DOI":"10.12688\/f1000research.16629.1"},{"key":"4172_CR86","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1146\/annurev-genet-120116-024704","volume":"51","author":"C Mayr","year":"2017","unstructured":"Mayr C (2017) Regulation by 3\u2032-Untranslated Regions. Annu Rev Genet 51:171\u2013194","journal-title":"Annu Rev Genet"},{"issue":"2","key":"4172_CR87","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1038\/ng1710","volume":"38","author":"JA Drake","year":"2006","unstructured":"Drake JA et al (2006) Conserved noncoding sequences are selectively constrained and not mutation cold spots. Nat Genet 38(2):223\u2013227","journal-title":"Nat Genet"},{"issue":"10","key":"4172_CR88","doi-asserted-by":"crossref","first-page":"2315","DOI":"10.1093\/nar\/21.10.2315","volume":"21","author":"L Duret","year":"1993","unstructured":"Duret L, Dorkeld F, Gautier C (1993) Strong conservation of non-coding sequences during vertebrates evolution: potential involvement in post-transcriptional regulation of gene expression. Nucleic Acids Res 21(10):2315\u20132322","journal-title":"Nucleic Acids Res"},{"issue":"6","key":"4172_CR89","doi-asserted-by":"crossref","first-page":"1991","DOI":"10.1111\/cas.14394","volume":"111","author":"YQ Li","year":"2020","unstructured":"Li YQ et al (2020) FNDC3B 3\u2032-UTR shortening escapes from microRNA-mediated gene repression and promotes nasopharyngeal carcinoma progression. Cancer Sci 111(6):1991\u20132003","journal-title":"Cancer Sci"},{"issue":"12","key":"4172_CR90","doi-asserted-by":"crossref","first-page":"1603","DOI":"10.1038\/cdd.2013.125","volume":"20","author":"E Mogilyansky","year":"2013","unstructured":"Mogilyansky E, Rigoutsos I (2013) The miR-17\/92 cluster: a comprehensive update on its genomics, genetics, functions and increasingly important and numerous roles in health and disease. Cell Death Differ 20(12):1603\u20131614","journal-title":"Cell Death Differ"},{"issue":"9","key":"4172_CR91","volume":"7","author":"LW Thomas","year":"2012","unstructured":"Thomas LW et al (2012) Serine 162, an essential residue for the mitochondrial localization, stability and anti-apoptotic function of Mcl-1. PLoS ONE 7(9):e45088","journal-title":"PLoS ONE"},{"issue":"7","key":"4172_CR92","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1016\/j.tcb.2020.03.004","volume":"30","author":"SJF Chong","year":"2020","unstructured":"Chong SJF et al (2020) Noncanonical cell fate regulation by Bcl-2 proteins. Trends Cell Biol 30(7):537\u2013555","journal-title":"Trends Cell Biol"},{"issue":"8","key":"4172_CR93","doi-asserted-by":"crossref","first-page":"e99748","DOI":"10.15252\/embj.201899748","volume":"38","author":"R Yu","year":"2019","unstructured":"Yu R et al (2019) Human Fis1 regulates mitochondrial dynamics through inhibition of the fusion machinery. EMBO J 38:e99748","journal-title":"EMBO J"},{"issue":"3","key":"4172_CR94","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1042\/EBC20170104","volume":"62","author":"L Tilokani","year":"2018","unstructured":"Tilokani L et al (2018) Mitochondrial dynamics: overview of molecular mechanisms. Essays Biochem 62(3):341\u2013360","journal-title":"Essays Biochem"},{"key":"4172_CR95","doi-asserted-by":"crossref","first-page":"572182","DOI":"10.3389\/fcell.2020.572182","volume":"8","author":"M Joaquim","year":"2020","unstructured":"Joaquim M, Escobar-Henriques M (2020) Role of mitofusins and mitophagy in life or death decisions. Front Cell Dev Biol 8:572182","journal-title":"Front Cell Dev Biol"},{"key":"4172_CR96","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1038\/s41420-019-0199-x","volume":"5","author":"M Milani","year":"2019","unstructured":"Milani M et al (2019) DRP-1 functions independently of mitochondrial structural perturbations to facilitate BH3 mimetic-mediated apoptosis. Cell Death Discov 5:117","journal-title":"Cell Death Discov"}],"container-title":["Cellular and Molecular Life Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00018-022-04172-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00018-022-04172-x\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00018-022-04172-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,18]],"date-time":"2023-11-18T06:54:54Z","timestamp":1700290494000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00018-022-04172-x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3]]},"references-count":96,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2022,3]]}},"alternative-id":["4172"],"URL":"https:\/\/doi.org\/10.1007\/s00018-022-04172-x","relation":{},"ISSN":["1420-682X","1420-9071"],"issn-type":[{"value":"1420-682X","type":"print"},{"value":"1420-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3]]},"assertion":[{"value":"17 August 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 January 2022","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 January 2022","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 March 2022","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare that there is no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"The isolation of immune cells from buffy coats of healthy blood donors was approved by the Centro Hospitalar Universit\u00e1rio S\u00e3o Jo\u00e3o Ethics Committee (protocol 90\/19), after each donor informed consent collection.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}}],"article-number":"164"}}