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Mol. Life Sci."],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Skeletal disorders are problematic aspects for the aquaculture industry as skeletal deformities, which affect most species of farmed fish, increase production costs and affect fish welfare. Following recent findings that show the presence of osteoactive compounds in marine organisms, we evaluated the osteogenic and mineralogenic potential of commercially available microalgae strains <jats:italic>Skeletonema costatum<\/jats:italic> and <jats:italic>Tetraselmis striata<\/jats:italic> CTP4 in several fish systems. Ethanolic extracts increased extracellular matrix mineralization in gilthead seabream (<jats:italic>Sparus aurata<\/jats:italic>) bone-derived cell cultures and promoted osteoblastic differentiation in zebrafish (<jats:italic>Danio rerio<\/jats:italic>) larvae. Long-term dietary exposure to both extracts increased bone mineralization in zebrafish and upregulated the expression of genes involved in bone formation (<jats:italic>sp7<\/jats:italic>, <jats:italic>col1a1a<\/jats:italic>, <jats:italic>oc1<\/jats:italic>, and <jats:italic>oc2<\/jats:italic>), bone remodeling (<jats:italic>acp5a<\/jats:italic>), and antioxidant defenses (<jats:italic>cat<\/jats:italic>, <jats:italic>sod1<\/jats:italic>). Extracts also improved the skeletal status of zebrafish juveniles by reducing the incidence of skeletal anomalies. Our results indicate that both strains of microalgae contain osteogenic and mineralogenic compounds, and that ethanolic extracts have the potential for an application in the aquaculture sector as dietary supplements to support fish bone health. Future studies should also identify osteoactive compounds and establish whether they can be used in human health to broaden the therapeutic options for bone erosive disorders such as osteoporosis.<\/jats:p>","DOI":"10.1007\/s00018-023-04953-y","type":"journal-article","created":{"date-parts":[[2023,9,30]],"date-time":"2023-09-30T17:01:36Z","timestamp":1696093296000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The osteogenic and mineralogenic potential of the microalgae Skeletonema costatum and Tetraselmis striata CTP4 in fish models"],"prefix":"10.1007","volume":"80","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2593-2499","authenticated-orcid":false,"given":"Alessio","family":"Carletti","sequence":"first","affiliation":[]},{"given":"Joana T.","family":"Rosa","sequence":"additional","affiliation":[]},{"given":"Katia","family":"Pes","sequence":"additional","affiliation":[]},{"given":"In\u00eas","family":"Borges","sequence":"additional","affiliation":[]},{"given":"Tam\u00e1ra","family":"Santos","sequence":"additional","affiliation":[]},{"given":"Lu\u00edsa","family":"Barreira","sequence":"additional","affiliation":[]},{"given":"Jo\u00e3o","family":"Varela","sequence":"additional","affiliation":[]},{"given":"Hugo","family":"Pereira","sequence":"additional","affiliation":[]},{"given":"M. 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The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"Procedures involving animals were performed following the EU and Portuguese legislation for animal experimentation and welfare (Directives 86\/609\/CEE and 2010\/63\/EU; Portaria 1005\/92, 466\/95 and 1131\/97; Decreto-Lei 113\/2013). Animal handling and experimentation were performed by qualified operators accredited by the Portuguese Dire\u00e7\u00e3o-Geral de Alimenta\u00e7\u00e3o e Veterin\u00e1ria under the authorization no. 012769\/2021. All efforts were made to minimize pain, distress, and discomfort. Experiments were terminated (fish were returned to normal conditions or euthanized) whenever adverse effects were observed.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"No human research participants were involved in the present study.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}}],"article-number":"310"}}