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However, the relative importance of epigenetic versus genetic changes in adaptation and how they may interact remains poorly understood. Here, we investigate the role of DNA methylation in adaptation by studying a population of Allis shad\u00a0(<jats:italic>Alosa alosa<\/jats:italic>)\u00a0that evolved a completely freshwater life history approximately 70\u00a0years ago and the anadromous one that founded it. Using reduced representation bisulfite sequencing, we identified 227 differentially methylated regions (DMRs) between them, overlapping known important genes for freshwater adaptation, such as ATP2B4, PRLH2, and KCNF1A. Enrichment analysis of GO terms suggested that genes in the identified DMRs play key roles in neural, growth, and developmental functions, which is concordant with previous studies on adaptation to freshwater in this species and genus. Using pool-seq data from an earlier study, we then tested if the DMRs for freshwater shad found here overlapped genomic outlier regions that may be under genetic selection in three independently evolved, freshwater populations (including the one studied here). Our analysis showed that the DMRs identified here fall broadly outside genomic regions under natural selection. However, 45% of these were associated with CpG\u2009&gt;\u2009TpG deamination events in DMRs, a mutation tightly linked with DNA methylation. Our study illustrates that both genetic and epigenetic mechanisms are important during the initial stages of adaptation in this system. It also supports the hypothesis that methylation may generate polymorphism that fuels adaptive evolution.<\/jats:p>","DOI":"10.1007\/s00239-025-10253-9","type":"journal-article","created":{"date-parts":[[2025,6,2]],"date-time":"2025-06-02T09:53:05Z","timestamp":1748857985000},"page":"406-422","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Adaptation to Freshwater in Allis Shad Involved a Combination of Genomic and Epigenomic Changes"],"prefix":"10.1007","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9519-7691","authenticated-orcid":false,"given":"Paulo","family":"Pereira","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7212-991X","authenticated-orcid":false,"given":"Sandra","family":"Afonso","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0417-8813","authenticated-orcid":false,"given":"Ant\u00f3nio","family":"M\u00farias","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9882-7775","authenticated-orcid":false,"given":"Miguel","family":"Carneiro","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2815-2375","authenticated-orcid":false,"given":"Stephen J.","family":"Sabatino","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2025,6,2]]},"reference":[{"issue":"10","key":"10253_CR1","doi-asserted-by":"publisher","first-page":"R87","DOI":"10.1186\/gb-2012-13-10-r87","volume":"13","author":"A Akalin","year":"2012","unstructured":"Akalin A, Kormaksson M, Li S, Garrett-Bakelman FE, Figueroa ME, Melnick A, Mason CE (2012) methylKit: a comprehensive R package for the analysis of genome-wide DNA methylation profiles. 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