{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T19:40:33Z","timestamp":1771702833258,"version":"3.50.1"},"reference-count":61,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2010,4,27]],"date-time":"2010-04-27T00:00:00Z","timestamp":1272326400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Appl Microbiol Biotechnol"],"published-print":{"date-parts":[[2010,7]]},"DOI":"10.1007\/s00253-010-2610-7","type":"journal-article","created":{"date-parts":[[2010,4,26]],"date-time":"2010-04-26T13:01:26Z","timestamp":1272286886000},"page":"1437-1446","source":"Crossref","is-referenced-by-count":33,"title":["Expression of Trichoderma reesei cellulases CBHI and EGI in Ashbya gossypii"],"prefix":"10.1007","volume":"87","author":[{"given":"Orqu\u00eddea","family":"Ribeiro","sequence":"first","affiliation":[]},{"given":"Marilyn","family":"Wiebe","sequence":"additional","affiliation":[]},{"given":"Marja","family":"Ilm\u00e9n","sequence":"additional","affiliation":[]},{"given":"Luc\u00edlia","family":"Domingues","sequence":"additional","affiliation":[]},{"given":"Merja","family":"Penttil\u00e4","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2010,4,27]]},"reference":[{"key":"2610_CR1","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1111\/j.1432-1033.1991.tb16227.x","volume":"200","author":"S Aho","year":"1991","unstructured":"Aho S, Olkkonen V, Jalava T, Paloheimo M, B\u00fchler R, Niku-Paavola ML, Bamford DH, Korhola M (1991) Monoclonal antibodies against core and cellulose-binding domains of Trichoderma reesei cellobiohydrolases I and II and endoglucanase I. Eur J Biochem 200:643\u2013649","journal-title":"Eur J Biochem"},{"key":"2610_CR2","unstructured":"Alth\u00f6efer H, Pompeus M, Revuelta JL, Santos M, Jiminez A, Benito R, Santamaria R, Fernandez J (2001) Protein production using Ashbya gossypii. Patent WO\/2001\/023576"},{"key":"2610_CR3","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1007\/BF01021247","volume":"14","author":"DB Archer","year":"1992","unstructured":"Archer DB, Mckenzie DA, Jeenes DJ, Roberts IN (1992) Proteolytic degradation of heterologous protein expressed in Aspergillus niger. Biotechnol Lett 14:357\u2013362","journal-title":"Biotechnol Lett"},{"key":"2610_CR4","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1093\/oxfordjournals.aob.a090018","volume":"40","author":"SF Ashby","year":"1926","unstructured":"Ashby SF, Nowell W (1926) The fungi of stigmatomycosis. Ann Bot 40:69\u201384","journal-title":"Ann Bot"},{"key":"2610_CR5","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1002\/1097-0290(20000905)69:5<486::AID-BIT3>3.0.CO;2-N","volume":"69","author":"H Boer","year":"2000","unstructured":"Boer H, Teeri T, Koiivula A (2000) Characterization of Trichoderma reesei cellobiohydrolase Cel7A secreted from Pichia pastoris using two different promoters. Biotechnol Bioeng 69:486\u2013494","journal-title":"Biotechnol Bioeng"},{"key":"2610_CR6","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/0168-1656(93)90156-H","volume":"31","author":"MP Broekhuijsen","year":"1993","unstructured":"Broekhuijsen MP, Mattern IE, Contreras R, Kinghorn JR, van den Hondel CAMJJ (1993) Secretion of heterologous proteins by Aspergillus niger: production of active human interleukin-6 in a protease-deficient mutant by KEX2-like processing of a glucoamylase-HIL6 fusion protein. J Biotechnol 31:135\u2013145","journal-title":"J Biotechnol"},{"key":"2610_CR7","volume-title":"Biodegradation of cellulose: enzymology and biotechnology","author":"AJ Clarke","year":"1997","unstructured":"Clarke AJ (1997) Biodegradation of cellulose: enzymology and biotechnology. Technomic Publishing, Lancaster"},{"key":"2610_CR8","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/S0304-4165(98)00132-9","volume":"1426","author":"N Dean","year":"1999","unstructured":"Dean N (1999) Asparagine-linked glycosylation in the yeast Golgi. Biochem Biophys Acta 1426:309\u2013322","journal-title":"Biochem Biophys Acta"},{"key":"2610_CR9","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1146\/annurev.mi.26.100172.002101","volume":"26","author":"AL Demain","year":"1972","unstructured":"Demain AL (1972) Riboflavin oversynthesis. Annu Rev Microbiol 26:369\u2013388","journal-title":"Annu Rev Microbiol"},{"key":"2610_CR10","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1093\/glycob\/cwn044","volume":"18","author":"N Deshpande","year":"2008","unstructured":"Deshpande N, Wilkins MR, Packer N, Nevalainen H (2008) Protein glycosylation pathways in filamentous fungi. Glycobiology 18:626\u2013637","journal-title":"Glycobiology"},{"key":"2610_CR11","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1126\/science.1095781","volume":"304","author":"FS Dietrich","year":"2004","unstructured":"Dietrich FS, Voegeli S, Brachat S, Lerch A, Gates K, Steiner S, Mohr C, P\u00f6hlmann R, Luedi P, Choi S, Wing RA, Flavier A, Gaffney TD, Philippsen P (2004) The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome. Science 304:304\u2013307","journal-title":"Science"},{"key":"2610_CR12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s00294-007-0134-1","volume":"52","author":"A D\u00fcnkler","year":"2007","unstructured":"D\u00fcnkler A, Wendland J (2007) Use of MET3 promoters for regulated gene expression in Ashbya gossypii. Curr Genet 52:1\u201310","journal-title":"Curr Genet"},{"key":"2610_CR13","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1007\/BF01570754","volume":"29","author":"HJ Flint","year":"1994","unstructured":"Flint HJ, Zhang J-X, Martin J (1994) Multiplicity and expression of xylanases in the rumen cellulolytic bacterium Ruminococcus flavefaciens. Curr Microbiol 29:139\u2013143","journal-title":"Curr Microbiol"},{"key":"2610_CR14","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/S0304-4165(98)00126-3","volume":"1426","author":"TR Gemmill","year":"1999","unstructured":"Gemmill TR, Trimble RB (1999) Overview of N- and O-linked oligosaccharide structures found in various yeast species. Biochem Biophys Acta 1426:227\u2013237","journal-title":"Biochem Biophys Acta"},{"key":"2610_CR15","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1002\/yea.320110408","volume":"11","author":"RD Gietz","year":"1995","unstructured":"Gietz RD, Schiestl RH, Willems AR, Woods RA (1995) Studies on the transformation of intact yeast cells by the LiAc\/SS-DNA\/PEG procedure. Yeast 11:355\u2013360","journal-title":"Yeast"},{"key":"2610_CR16","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1021\/bp9901116","volume":"15","author":"S Godbole","year":"1999","unstructured":"Godbole S, Decker SR, Nieves RA, Adney WS, Vinzant TB, Baker JO, Thomas SR, Himmel ME (1999) Cloning and expression of Trichoderma reesei cellobiohydrolase I in Pichia pastoris. Biotechnol Prog 15:828\u2013833","journal-title":"Biotechnol Prog"},{"key":"2610_CR17","doi-asserted-by":"crossref","first-page":"2519","DOI":"10.1093\/nar\/24.13.2519","volume":"24","author":"U G\u00fcldener","year":"1996","unstructured":"G\u00fcldener U, Heck S, Fielder T, Beinhauer J, Hegemann JH (1996) A new efficient gene disruption cassette for repeated use in budding yeast. Nucleic Acids Res 24:2519\u20132524","journal-title":"Nucleic Acids Res"},{"key":"2610_CR18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1754-6834-1-10","volume":"1","author":"T Jeoh","year":"2008","unstructured":"Jeoh T, Michener W, Himmel ME, Decker SR, Adney WS (2008) Implications of cellobiohydrolase glycosylation for use in biomass conversion. Biotechnol Biofuels 1:1\u201310","journal-title":"Biotechnol Biofuels"},{"key":"2610_CR19","doi-asserted-by":"crossref","first-page":"5743","DOI":"10.1128\/AEM.71.10.5743-5751.2005","volume":"71","author":"A Jim\u00e9nez","year":"2005","unstructured":"Jim\u00e9nez A, Santos MA, Pompejus M, Revuelta JL (2005) Metabolic engineering of the purine pathway for riboflavin production in Ashbya gossypii. Appl Environ Microbiol 71:5743\u20135751","journal-title":"Appl Environ Microbiol"},{"key":"2610_CR20","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1038\/287869a0","volume":"287","author":"A Jimenez","year":"1980","unstructured":"Jimenez A, Davies J (1980) Expression of a transposable antibiotic resistance element in Saccharomyces. Nature 287:869\u2013871","journal-title":"Nature"},{"key":"2610_CR21","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1007\/BF03028632","volume":"12","author":"S Kanamasa","year":"2007","unstructured":"Kanamasa S, Tajima S, Park EY (2007) Isocitrate dehydrogenase and isocitrate lyase are essential enzymes for riboflavin production in Ashbya gossypii. Biotechnol Bioprocess Eng 12:92\u201399","journal-title":"Biotechnol Bioprocess Eng"},{"key":"2610_CR22","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.jbiotec.2004.03.025","volume":"113","author":"M Karos","year":"2004","unstructured":"Karos M, Vilarino C, Bollschweiler C, Revuelta JL (2004) A genome-wide transcription analysis of a fungal riboflavin overproducer. J Biotechnol 113:69\u201376","journal-title":"J Biotechnol"},{"key":"2610_CR23","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1007\/s00253-005-0124-5","volume":"71","author":"T Kato","year":"2005","unstructured":"Kato T, Park EY (2005) Expression of alanine:glyoxylate aminotransferase gene from Saccharomyces cerevisiae in Ashbya gossypii. Appl Microbiol Biotechnol 71:46\u201352","journal-title":"Appl Microbiol Biotechnol"},{"key":"2610_CR24","first-page":"1","volume":"45","author":"CP Kubicek","year":"1992","unstructured":"Kubicek CP (1992) The cellulase proteins of Trichoderma reesei: structure, multiplicity, mode of action and regulation of formation. Adv Biochem Eng Biotechnol 45:1\u201325","journal-title":"Adv Biochem Eng Biotechnol"},{"key":"2610_CR25","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/0141-0229(93)90030-6","volume":"15","author":"CP Kubicek","year":"1993","unstructured":"Kubicek CP, Messner R, Gruber F, Mach RL, Kubicek-Pranz EM (1993) The Trichoderma cellulase regulatory puzzle: from the interior life of a secretory fungus. Enzyme Microb Technol 15:90\u201399","journal-title":"Enzyme Microb Technol"},{"key":"2610_CR26","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","volume":"227","author":"UK Laemmli","year":"1970","unstructured":"Laemmli UK (1970) Cleavage of structural proteins during assembly of head of bacteriophage T4. Nature 227:680\u2013685","journal-title":"Nature"},{"issue":"58","key":"2610_CR27","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1007\/BF02941718","volume":"57","author":"RA Laymon","year":"1996","unstructured":"Laymon RA, Adney WS, Mohagheghi A, Himmel ME, Thomas SR (1996) Cloning and expression of full-length Trichoderma reesei cellobiohydrolase I cDNAs in Escherichia coli. Appl Biochem Biotech 57(58):389\u2013400","journal-title":"Appl Biochem Biotech"},{"key":"2610_CR28","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1006\/prep.2001.1395","volume":"21","author":"Z Li","year":"2001","unstructured":"Li Z, Xiong F, Lin Q, d'Anjou M, Daugulis AJ, Yang DS, Hew CL (2001) Low-temperature increases the yield of biologically active herring antifreeze protein in Pichia pastoris. Protein Expr Purif 21:438\u2013445","journal-title":"Protein Expr Purif"},{"key":"2610_CR29","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/0003-2697(89)90115-2","volume":"180","author":"F Maley","year":"1989","unstructured":"Maley F, Trimble RB, Tarentino AL, Plummer TH Jr (1989) Characterization of glycoproteins and their associated oligosaccharides through the use of endoglycosidases. Anal Biochem 180:195\u2013204","journal-title":"Anal Biochem"},{"key":"2610_CR30","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1111\/j.1432-1033.1997.00617.x","volume":"245","author":"M Maras","year":"1997","unstructured":"Maras M, De Bruyn A, Schraml J, Herdewijn P, Claeyssens M, Fiers W, Contreras R (1997) Structural characterization of N-linked oligosaccharides from cellobiohydrolase I secreted by the filamentous fungus Trichoderma reesei RUTC 30. Eur J Biochem 245:617\u2013625","journal-title":"Eur J Biochem"},{"key":"2610_CR31","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1023\/A:1026436424881","volume":"16","author":"M Maras","year":"1999","unstructured":"Maras M, van Die I, Contreras R, van den Hondel CA (1999) Filamentous fungi as production organisms for glycoproteins of bio-medical interest. Glycoconj J 16:99\u2013107","journal-title":"Glycoconj J"},{"key":"2610_CR32","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1385\/ABAB:87:1:1","volume":"87","author":"CS Park","year":"2000","unstructured":"Park CS, Chang CC, Ryu DD (2000) Expression and high-level secretion of Trichoderma reesei endoglucanase I in Yarrowia lipolytica. Appl Biochem Biotechnol 87:1\u201315","journal-title":"Appl Biochem Biotechnol"},{"key":"2610_CR33","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/0378-1119(87)90110-7","volume":"61","author":"M Penttil\u00e4","year":"1987","unstructured":"Penttil\u00e4 M, Nevalainen H, R\u00e4tt\u00f6 M, Salminen E, Knowles J (1987a) A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei. Gene 61:155\u2013164","journal-title":"Gene"},{"key":"2610_CR34","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1002\/yea.320030305","volume":"3","author":"ME Penttil\u00e4","year":"1987","unstructured":"Penttil\u00e4 ME, Andr\u00e9 L, Saloheimo M, Lehtovaara P, Knowles JK (1987b) Expression of two Trichoderma reesei endoglucanases in the yeast Saccharomyces cerevisiae. Yeast 3:175\u2013185","journal-title":"Yeast"},{"key":"2610_CR35","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/0378-1119(88)90549-5","volume":"63","author":"ME Penttil\u00e4","year":"1988","unstructured":"Penttil\u00e4 ME, Andre L, Lehtovaara P, Bailey M, Teeri TT, Knowles JKC (1988) Efficient secretion of two fungal cellobiohydrolases by Saccharomyces cerevisiae. Gene 63:103\u2013112","journal-title":"Gene"},{"key":"2610_CR36","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1093\/glycob\/1.3.257","volume":"1","author":"TH Plummer Jr","year":"1991","unstructured":"Plummer TH Jr, Tarentino AL (1991) Purification of the oligosaccharide-cleaving enzymes of Flavobacterium meningosepticum. Glycobiology 1:257\u2013263","journal-title":"Glycobiology"},{"key":"2610_CR37","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1002\/prot.340140408","volume":"14","author":"T Reinikainen","year":"1992","unstructured":"Reinikainen T, Rouhonen L, Nevanen T, Laaksonen L, Kraulis P, Jones TA, Knowles J, Teeri T (1992) Investigation of the function of mutated cellulose-binding domains of Trichoderma reesei cellobiohydrolase I. Proteins Struct Funct Genet 14:475\u2013482","journal-title":"Proteins Struct Funct Genet"},{"key":"2610_CR38","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/0378-1119(92)90043-O","volume":"122","author":"IN Roberts","year":"1992","unstructured":"Roberts IN, Jeenes DJ, MacKenzie DA, Wilkinson AP, Sumner IG, Archer DB (1992) Heterologous gene expression in A. niger: a glucoamylase-porcine pancreatic prophospholipase A2 fusion protein is secreted and processed to yield mature enzyme. Gene 122:155\u2013161","journal-title":"Gene"},{"key":"2610_CR39","first-page":"1479","volume":"13","author":"JC Royer","year":"1995","unstructured":"Royer JC, Moyer DL, Reiwitch SG, Madden MS, Jensen EB, Brown SH, Yonker CC, Johnston JA, Golightly EJ, Yoder WT, Shuster JR (1995) Fusarium graminearum A 3\/5 as a novel host for heterologous protein production. Biotechnology (NY) 13:1479\u20131483","journal-title":"Biotechnology (NY)"},{"key":"2610_CR40","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/0168-1656(95)00024-K","volume":"39","author":"L Ruohonen","year":"1995","unstructured":"Ruohonen L, Aalto MK, Ker\u00e4nen S (1995) Modifications to the ADH1 promoter of Saccharomyces cerevisiae for efficient production of heterologous proteins. J Biotechnol 39:193\u2013203","journal-title":"J Biotechnol"},{"key":"2610_CR41","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1111\/j.1365-2958.1994.tb00417.x","volume":"13","author":"A Saloheimo","year":"1994","unstructured":"Saloheimo A, Henrissat B, Hoffr\u00e9n AM, Teleman O, Penttil\u00e4 M (1994) A novel, small endoglucanase gene, egl5, from Trichoderma reesei isolated by expression in yeast. Mol Microbiol 13:219\u2013228","journal-title":"Mol Microbiol"},{"key":"2610_CR42","volume-title":"Methods in yeast genetics","author":"F Sherman","year":"1986","unstructured":"Sherman F, Fink GR, Hicks JB (1986) Methods in yeast genetics. Cold Spring Harbor LabPress, Plainview"},{"key":"2610_CR43","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/S1046-5928(02)00706-4","volume":"28","author":"X Shi","year":"2003","unstructured":"Shi X, Karkut T, Chamankhah M, Alting-Mees M, Hemmingsen SM, Hegedus D (2003) Optimal conditions for the expression of a single-chain antibody (scFv) gene in Pichia pastoris. Protein Expr Purif 28:321\u2013330","journal-title":"Protein Expr Purif"},{"key":"2610_CR44","doi-asserted-by":"crossref","first-page":"20756","DOI":"10.1016\/S0021-9258(19)36847-4","volume":"268","author":"M Srisodsuk","year":"1993","unstructured":"Srisodsuk M, Reinikainen T, Penttil\u00e4 M, Teeri TT (1993) Role of the interdomain linker peptide of Trichoderma reesei cellobiohydrolase I in its interaction with crystalline cellulose. J Biol Chem 268:20756\u201320761","journal-title":"J Biol Chem"},{"key":"2610_CR45","doi-asserted-by":"crossref","unstructured":"Stahmann KP, B\u00f6ddecker T, Sahm H (1997) Regulation and properties of a fungal lipase showing interfacial inactivation by gas bubbles, or droplets of lipid or fatty acid. Eur J Biochem 244:220\u2013225","DOI":"10.1111\/j.1432-1033.1997.00220.x"},{"key":"2610_CR46","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1093\/glycob\/cwh080","volume":"14","author":"I Stals","year":"2004","unstructured":"Stals I, Sandra K, Geysens S, Contreras R, Van Beeumen J, Claeyssens M (2004) Factors influencing glycosylation of Trichoderma reesei cellulases. I: postsecretorial changes of the O- and N-glycosylation pattern of Cel7A. Glycobiology 14:713\u2013724","journal-title":"Glycobiology"},{"key":"2610_CR47","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1093\/genetics\/140.3.973","volume":"140","author":"S Steiner","year":"1995","unstructured":"Steiner S, Wendland J, Wright MC, Philippsen P (1995) Homologous recombination as the main mechanism for DNA integration and cause of rearrangements in the filamentous ascomycete Ashbya gossypii. Genetics 140:973\u2013987","journal-title":"Genetics"},{"key":"2610_CR48","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/S0168-1656(98)00084-4","volume":"65","author":"S Takashima","year":"1998","unstructured":"Takashima S, Iikura H, Nakamura A, Hidaka M, Masaki H, Uozumi T (1998) Overproduction of recombinant Trichoderma reesei cellulases by Aspergillus oryzae and their enzymatic properties. J Biotechnol 65:163\u2013171","journal-title":"J Biotechnol"},{"key":"2610_CR49","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1128\/AEM.43.4.777-780.1982","volume":"43","author":"RM Teather","year":"1982","unstructured":"Teather RM, Wood PJ (1982) Use of Congo red-polysaccharide interactions in enumeration and characterisation of cellulolytic bacteria from bovine rumen. Appl Environ Microbiol 43:777\u2013780","journal-title":"Appl Environ Microbiol"},{"key":"2610_CR50","unstructured":"Teeri TT (1987) The cellulolytic enzyme system of Trichoderma reesei. Dissertation, University of Helsinki, VTT Publication No. 38"},{"key":"2610_CR51","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/0003-2697(87)90367-8","volume":"164","author":"TT Teeri","year":"1987","unstructured":"Teeri TT, Kumar V, Lehtovaara P, Knowles J (1987) Construction of cDNA libraries by blunt-end ligation: high-frequency cloning of long cDNAs from filamentous fungi. Anal Biochem 164:60\u201367","journal-title":"Anal Biochem"},{"key":"2610_CR52","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1016\/0003-2697(84)90080-0","volume":"141","author":"RB Trimble","year":"1984","unstructured":"Trimble RB, Maley F (1984) Optimizing hydrolysis of N-linked high-mannose oligosaccharides by endo-beta-N-acetylglucosaminidase H. Anal Biochem 141:515\u2013522","journal-title":"Anal Biochem"},{"key":"2610_CR53","first-page":"60","volume":"5","author":"JN Arsdell Van","year":"1987","unstructured":"Van Arsdell JN, Kwokl S, Schweickart VL, Ladner MB, Gelfandl DH, Innisl MA (1987) Cloning, characterization and expression in Saccharomyces cerevisiae endoglucanase I from Trichoderma reesei. Bio\/technology 5:60\u201364","journal-title":"Bio\/technology"},{"key":"2610_CR54","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1016\/S0167-7799(97)01020-2","volume":"15","author":"JP Hombergh van den","year":"1997","unstructured":"van den Hombergh JP, van de Vondervoort PJ, Fraissinet TL, Visser J (1997) Aspergillus as a host for heterologous protein production: the problem of proteases. Trends Biotechnol 15:256\u2013263","journal-title":"Trends Biotechnol"},{"key":"2610_CR55","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1006\/bbrc.1997.6391","volume":"233","author":"I Ossowski von","year":"1997","unstructured":"von Ossowski I, Teeri T, Kalkkinen N, Oker-Blom C (1997) Expression of a fungal cellobiohydrolase in insect cells. Biochem Biophys Res Commun 233:25\u201329","journal-title":"Biochem Biophys Res Commun"},{"key":"2610_CR56","doi-asserted-by":"crossref","first-page":"1793","DOI":"10.1002\/yea.320101310","volume":"10","author":"A Wach","year":"1994","unstructured":"Wach A, Brachat A, P\u00f6hlmann R, Philippsen P (1994) New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae. Yeast 10:1793\u20131808","journal-title":"Yeast"},{"key":"2610_CR57","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/0378-1119(83)90194-4","volume":"26","author":"TD Webster","year":"1983","unstructured":"Webster TD, Dickson RC (1983) Direct selection of Saccharomyces cerevisiae resistant to the antibiotic G418 following transformation with a DNA vector carrying the kanamycin-resistance gene of Tn903. Gene 26:243\u2013252","journal-title":"Gene"},{"key":"2610_CR58","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1007\/s002940050460","volume":"35","author":"J Wendland","year":"1999","unstructured":"Wendland J, Pohlmann R, Dietrich F, Steiner S, Mohr C, Philippsen P (1999) Compact organization of rRNA genes in the filamentous fungus Ashbya gossypii. Curr Genet 35:618\u2013625","journal-title":"Curr Genet"},{"key":"2610_CR59","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1016\/S0378-1119(99)00509-0","volume":"242","author":"J Wendland","year":"2000","unstructured":"Wendland J, Ayad-Durieux Y, Knechtle P, Rebischung C, Philippsen P (2000) PCR-based gene targeting in the filamentous fungus Ashbya gossypii. Gene 242:381\u2013391","journal-title":"Gene"},{"key":"2610_CR60","first-page":"95","volume":"9","author":"L Wickerham","year":"1946","unstructured":"Wickerham L, Flickinger MH, Johnston RM (1946) The production of riboflavin by Ashbya gossypii. Arch Biochem 9:95\u201398","journal-title":"Arch Biochem"},{"key":"2610_CR61","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/0378-1119(91)90593-Z","volume":"109","author":"MC Wright","year":"1991","unstructured":"Wright MC, Philippsen P (1991) Replicative transformation of the filamentous fungus Ashbya gossypii with plasmids containing Saccharomyces cerevisiae ARS elements. Gene 109:99\u2013105","journal-title":"Gene"}],"container-title":["Applied Microbiology and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-010-2610-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00253-010-2610-7\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-010-2610-7","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,26]],"date-time":"2021-10-26T19:10:54Z","timestamp":1635275454000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00253-010-2610-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,4,27]]},"references-count":61,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2010,7]]}},"alternative-id":["2610"],"URL":"https:\/\/doi.org\/10.1007\/s00253-010-2610-7","relation":{},"ISSN":["0175-7598","1432-0614"],"issn-type":[{"value":"0175-7598","type":"print"},{"value":"1432-0614","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,4,27]]}}}