{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T08:36:13Z","timestamp":1770712573068,"version":"3.49.0"},"reference-count":55,"publisher":"Springer Science and Business Media LLC","issue":"16","license":[{"start":{"date-parts":[[2023,6,29]],"date-time":"2023-06-29T00:00:00Z","timestamp":1687996800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2023,6,29]],"date-time":"2023-06-29T00:00:00Z","timestamp":1687996800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100008530","name":"FEDER","doi-asserted-by":"crossref","award":["Norte-01-0145-FEDER-000011-RL1\u2013QUALIFOOD"],"award-info":[{"award-number":["Norte-01-0145-FEDER-000011-RL1\u2013QUALIFOOD"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"crossref"}]},{"name":"FCT","award":["UID\/Multi\/50016\/2019"],"award-info":[{"award-number":["UID\/Multi\/50016\/2019"]}]},{"name":"FCT","award":["SFRH\/BD\/105304\/2014"],"award-info":[{"award-number":["SFRH\/BD\/105304\/2014"]}]},{"name":"FCT","award":["IF\/00588\/2015"],"award-info":[{"award-number":["IF\/00588\/2015"]}]},{"name":"Universidade Cat\u00f3lica Portuguesa"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Appl Microbiol Biotechnol"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:sec>\n                <jats:title>Abstract\n<\/jats:title>\n                <jats:p><jats:italic>Nannochloropsis oculata<\/jats:italic> is naturally rich in eicosapentaenoic acid (EPA). To turn this microalga into an economically viable source for commercial applications, extraction efficiency must be achieved. Pursuing this goal, emerging technologies such as high hydrostatic pressure (HHP) and moderate electric fields (MEF) were tested, aiming to increase EPA accessibility and subsequent extraction yields. The innovative approach used in this study combined these technologies and associated tailored, less hazardous different solvent mixtures (SM) with distinct polarity indexes. Although the classical Folch SM with chloroform:\u2009methanol (PI 4.4) provided the highest yield concerning total lipids (166.4\u00a0mg<jats:sub>lipid<\/jats:sub>\/g<jats:sub>biomass<\/jats:sub>), diethyl ether:\u2009ethanol (PI 3.6) presented statistically higher values in terms of EPA per biomass, corresponding to 1.3-fold increase. When SM were used in HHP and MEF, neither technology independently improved EPA extraction yields, although the sequential combination of technologies did result in 62% increment in EPA extraction. Overall, the SM and extraction methodologies tested (HHP\u2014200\u00a0MPa, 21\u00a0\u00b0C, 15\u00a0min, followed by MEF processing at 40\u00a0\u00b0C, 15\u00a0min) enabled increased EPA extraction yields from wet <jats:italic>N. oculata<\/jats:italic> biomass. These findings are of high relevance for the food and pharmaceutical industries, providing viable alternatives to the \u201cclassical\u201d extraction methodologies and solvents, with increased yields and lower environmental impact.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Key points<\/jats:title>\n                <jats:p>\u2022 <jats:italic>Et<\/jats:italic><jats:sub><jats:italic>2<\/jats:italic><\/jats:sub><jats:italic>O:\u2009EtOH is a less toxic and more efficient alternative to Folch solvent mixture<\/jats:italic><\/jats:p>\n                <jats:p>\u2022 <jats:italic>HHP or MEF per se was not able to significantly increase EPA extraction yield<\/jats:italic><\/jats:p>\n                <jats:p>\u2022 <jats:italic>Combinations of HHP and MEF technologies increased both lipids and EPA yields<\/jats:italic><\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Graphical abstract<\/jats:title>\n                \n              <\/jats:sec>","DOI":"10.1007\/s00253-023-12626-w","type":"journal-article","created":{"date-parts":[[2023,6,29]],"date-time":"2023-06-29T09:02:24Z","timestamp":1688029344000},"page":"5063-5077","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Combining high pressure and electric fields towards Nannochloropsis oculata eicosapentaenoic acid-rich extracts"],"prefix":"10.1007","volume":"107","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6205-1266","authenticated-orcid":false,"given":"S\u00e9rgio","family":"Sousa","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5967-4200","authenticated-orcid":false,"given":"Ana P.","family":"Carvalho","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5742-706X","authenticated-orcid":false,"given":"Carlos A.","family":"Pinto","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5214-2754","authenticated-orcid":false,"given":"Renata A.","family":"Amaral","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5536-6056","authenticated-orcid":false,"given":"Jorge A.","family":"Saraiva","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1553-9693","authenticated-orcid":false,"given":"Ricardo N.","family":"Pereira","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3593-8878","authenticated-orcid":false,"given":"Ant\u00f3nio A.","family":"Vicente","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1430-9370","authenticated-orcid":false,"given":"Ana C.","family":"Freitas","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7883-2446","authenticated-orcid":false,"given":"Ana M.","family":"Gomes","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2023,6,29]]},"reference":[{"key":"12626_CR1","doi-asserted-by":"publisher","first-page":"3879","DOI":"10.1039\/c6gc00611f","volume":"18","author":"CM Alder","year":"2016","unstructured":"Alder CM, Hayler JD, Henderson RK, Redman AM, Shukla L, Shuster LE, Sneddon HF (2016) Updating and further expanding GSK\u2019s solvent sustainability guide. Green Chem 18:3879\u20133890. https:\/\/doi.org\/10.1039\/c6gc00611f","journal-title":"Green Chem"},{"key":"12626_CR2","doi-asserted-by":"publisher","first-page":"319","DOI":"10.1002\/ente.201500242","volume":"4","author":"M Ali","year":"2016","unstructured":"Ali M, Watson IA (2016) Microwave thermolysis and lipid recovery from dried microalgae powder for biodiesel production. Energy Technol 4:319\u2013330. https:\/\/doi.org\/10.1002\/ente.201500242","journal-title":"Energy Technol"},{"key":"12626_CR3","doi-asserted-by":"publisher","first-page":"929","DOI":"10.1016\/j.cej.2012.11.063","volume":"215\u2013216","author":"RK Balasubramanian","year":"2013","unstructured":"Balasubramanian RK, Yen Doan TT, Obbard JP (2013) Factors affecting cellular lipid extraction from marine microalgae. Chem Eng J 215\u2013216:929\u2013936. https:\/\/doi.org\/10.1016\/j.cej.2012.11.063","journal-title":"Chem Eng J"},{"key":"12626_CR4","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1016\/j.supflu.2015.11.003","volume":"108","author":"FJL Baumgardt","year":"2016","unstructured":"Baumgardt FJL, Filho AZ, Brandalize MV, Da Costa DC, AntoniosiFilho NR, Abreu PCOV, Corazza ML, Ramos LP (2016) Lipid content and fatty acid profile of Nannochloropsis oculata before and after extraction with conventional solvents and\/or compressed fluids. J Supercrit Fluids 108:89\u201395. https:\/\/doi.org\/10.1016\/j.supflu.2015.11.003","journal-title":"J Supercrit Fluids"},{"key":"12626_CR5","doi-asserted-by":"publisher","first-page":"911","DOI":"10.1139\/cjm2014-0700","volume":"37","author":"E Bligh","year":"1959","unstructured":"Bligh E, Dyer W (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911\u2013917. https:\/\/doi.org\/10.1139\/cjm2014-0700","journal-title":"Can J Biochem Physiol"},{"key":"12626_CR6","doi-asserted-by":"publisher","first-page":"751","DOI":"10.1016\/j.foodchem.2018.11.106","volume":"278","author":"V Briones-Labarca","year":"2019","unstructured":"Briones-Labarca V, Giovagnoli-Vicu\u00f1a C, Ca\u00f1as-Saraz\u00faa R (2019) Optimization of extraction yield, flavonoids and lycopene from tomato pulp by high hydrostatic pressure-assisted extraction. Food Chem 278:751\u2013759. https:\/\/doi.org\/10.1016\/j.foodchem.2018.11.106","journal-title":"Food Chem"},{"key":"12626_CR7","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.ifset.2020.102532","volume":"66","author":"M Bueno","year":"2020","unstructured":"Bueno M, Gallego R, Chourio AM, Ib\u00e1\u00f1ez E, Herrero M, Salda\u00f1a MDA (2020) Green ultra-high pressure extraction of bioactive compounds from Haematococcus pluvialis and Porphyridium cruentum microalgae. Innov Food Sci Emerg Technol 66:1\u201310. https:\/\/doi.org\/10.1016\/j.ifset.2020.102532","journal-title":"Innov Food Sci Emerg Technol"},{"key":"12626_CR8","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s41061-018-0191-6","volume":"376","author":"FG Calvo-Flores","year":"2018","unstructured":"Calvo-Flores FG, Monteagudo-Arrebola MJ, Dobado JA, Isac-Garc\u00eda J (2018) Green and Bio-based solvents. Top Curr Chem 376:1\u201340. https:\/\/doi.org\/10.1007\/s41061-018-0191-6","journal-title":"Top Curr Chem"},{"key":"12626_CR9","doi-asserted-by":"publisher","first-page":"927","DOI":"10.1039\/b617536h","volume":"9","author":"C Capello","year":"2007","unstructured":"Capello C, Fischer U, Hungerb\u00fchler K (2007) What is a green solvent? A comprehensive framework for the environmental assessment of solvents. Green Chem 9:927\u2013934. https:\/\/doi.org\/10.1039\/b617536h","journal-title":"Green Chem"},{"key":"12626_CR10","doi-asserted-by":"publisher","first-page":"385","DOI":"10.1166\/jbmb.2011.1157","volume":"5","author":"M Chen","year":"2011","unstructured":"Chen M, Chen X, Liu T, Zhang W (2011) Subcritical ethanol extraction of lipid from wet microalgae paste of Nannochloropsis sp. J Biobased Mater Bioenergy 5:385\u2013389. https:\/\/doi.org\/10.1166\/jbmb.2011.1157","journal-title":"J Biobased Mater Bioenergy"},{"key":"12626_CR11","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1016\/S0963-9969(00)00187-3","volume":"34","author":"D Chevalier","year":"2001","unstructured":"Chevalier D, Le Bail A, Ghoul M (2001) Effects of high pressure treatment (100\u2013200 MPa) at low temperature on turbot (Scophthalmus maximus) muscle. Food Res Int 34:425\u2013429. https:\/\/doi.org\/10.1016\/S0963-9969(00)00187-3","journal-title":"Food Res Int"},{"key":"12626_CR12","doi-asserted-by":"publisher","first-page":"334","DOI":"10.1016\/0014-4827(69)90514-X","volume":"58","author":"WM Darley","year":"1969","unstructured":"Darley WM, Volcani BE (1969) Role of silicon in diatom me-tabolism. A silicon requirement for deoxyribonucleic acid synthesis in the diatom Cylindrotheca fusiformis Reimann and Lewin. Exp Cell Res 58:334\u2013342","journal-title":"Exp Cell Res"},{"key":"12626_CR13","doi-asserted-by":"publisher","unstructured":"De Carvalho Neto RG, Da Silva Do Nascimento JG, Costa MC, Lopes AC, Abdala Neto EF, Rossas Mota Filho C, Dos Santos AB (2014) Microalgae harvesting and cell disruption: a preliminary evaluation of the technology electroflotation by alternating current. Water Sci Technol 70:315\u2013320https:\/\/doi.org\/10.2166\/wst.2014.220","DOI":"10.2166\/wst.2014.220"},{"key":"12626_CR14","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1016\/j.ifset.2015.02.005","volume":"29","author":"CF De Oliveira","year":"2015","unstructured":"De Oliveira CF, Giordani D, Gurak PD, Cladera-Olivera F, Marczak LDF (2015) Extraction of pectin from passion fruit peel using moderate electric field and conventional heating extraction methods. Innov Food Sci Emerg Technol 29:201\u2013208. https:\/\/doi.org\/10.1016\/j.ifset.2015.02.005","journal-title":"Innov Food Sci Emerg Technol"},{"key":"12626_CR15","doi-asserted-by":"publisher","first-page":"762","DOI":"10.1016\/j.renene.2013.10.038","volume":"63","author":"F de Souza","year":"2014","unstructured":"de Souza F, Silva AP, Costa MC, Colzi Lopes A, Fares Abdala Neto E, Carrh\u00e1Leit\u00e3o R, Mota CR, Bezerra dos Santos A (2014) Comparison of pretreatment methods for total lipids extraction from mixed microalgae. Renew Energy 63:762\u2013766. https:\/\/doi.org\/10.1016\/j.renene.2013.10.038","journal-title":"Renew Energy"},{"key":"12626_CR16","doi-asserted-by":"publisher","first-page":"2139","DOI":"10.1007\/s12649-018-0253-6","volume":"10","author":"MP de Souza","year":"2019","unstructured":"de Souza MP, Hoeltz M, Gressler PD, Benitez LB, Schneider RCS (2019) Potential of microalgal bioproducts: general perspectives and main challenges. Waste Biomass Valor 10:2139\u20132156. https:\/\/doi.org\/10.1007\/s12649-018-0253-6","journal-title":"Waste Biomass Valor"},{"key":"12626_CR17","doi-asserted-by":"publisher","first-page":"2901","DOI":"10.1109\/TPS.2013.2274805","volume":"41","author":"C Eing","year":"2013","unstructured":"Eing C, Goettel M, Straessner R, Gusbeth C, Frey W (2013) Pulsed electric field treatment of microalgae - benefits for microalgae biomass processing. IEEE Trans Plasma Sci 41:2901\u20132907. https:\/\/doi.org\/10.1109\/TPS.2013.2274805","journal-title":"IEEE Trans Plasma Sci"},{"key":"12626_CR18","doi-asserted-by":"publisher","first-page":"8816","DOI":"10.1021\/acssuschemeng.9b00780","volume":"7","author":"P Ferreira-Santos","year":"2019","unstructured":"Ferreira-Santos P, Genisheva Z, Pereira RN, Teixeira JA, Rocha CMR (2019) Moderate electric fields as a potential tool for sustainable recovery of phenolic compounds from Pinus pinaster b ark. ACS Sustain Chem Eng 7:8816\u20138826. https:\/\/doi.org\/10.1021\/acssuschemeng.9b00780","journal-title":"ACS Sustain Chem Eng"},{"key":"12626_CR19","doi-asserted-by":"publisher","first-page":"497","DOI":"10.1016\/j.ultrasmedbio.2011.03.005","volume":"55","author":"J Folch","year":"1957","unstructured":"Folch J, Lees M, Sloane Stanley GH (1957) A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 55:497\u2013509. https:\/\/doi.org\/10.1016\/j.ultrasmedbio.2011.03.005","journal-title":"J Biol Chem"},{"key":"12626_CR20","doi-asserted-by":"publisher","unstructured":"Gallego R, Bueno M, Chourio AM, Ib\u00e1\u00f1ez E, Salda\u00f1a MDA, Herrero M (2021) Use of high and ultra-high pressure based-processes for the effective recovery of bioactive compounds from Nannochloropsis oceanica microalgae. J Supercrit Fluids 167. https:\/\/doi.org\/10.1016\/j.supflu.2020.105039","DOI":"10.1016\/j.supflu.2020.105039"},{"key":"12626_CR21","doi-asserted-by":"publisher","first-page":"1040","DOI":"10.1111\/1541-4337.12362","volume":"17","author":"M Gavahian","year":"2018","unstructured":"Gavahian M, Chu YH, Sastry S (2018) Extraction from Food and natural products by moderate electric field: mechanisms, benefits, and potential industrial applications. Compr Rev Food Sci Food Saf 17:1040\u20131052. https:\/\/doi.org\/10.1111\/1541-4337.12362","journal-title":"Compr Rev Food Sci Food Saf"},{"key":"12626_CR22","doi-asserted-by":"publisher","first-page":"656","DOI":"10.1016\/j.rser.2018.06.059","volume":"94","author":"P Geada","year":"2018","unstructured":"Geada P, Rodrigues R, Loureiro L, Pereira R, Fernandes B, Teixeira JA, Vasconcelos V, Vicente AA (2018) Electrotechnologies applied to microalgal biotechnology \u2013 applications, techniques and future trends. Renew Sustain Energy Rev 94:656\u2013668. https:\/\/doi.org\/10.1016\/j.rser.2018.06.059","journal-title":"Renew Sustain Energy Rev"},{"key":"12626_CR23","first-page":"1","volume":"1","author":"GPS Safety Summary","year":"2015","unstructured":"GPS Safety Summary (2015) Global Product Strategy ( GPS ) safety summary. Hell Pertoleum 1:1\u20135","journal-title":"Hell Pertoleum"},{"key":"12626_CR24","doi-asserted-by":"publisher","first-page":"179","DOI":"10.3762\/bjoc.12.20","volume":"12","author":"DI H\u01ced\u01ceruga","year":"2016","unstructured":"H\u01ced\u01ceruga DI, \u00dcnl\u00fcsayin M, Gruia AT, Bir\u01ceu C, Rusu G, H\u01ced\u01ceruga NG (2016) Thermal and oxidative stability of Atlantic salmon oil (Salmo salar L.) and complexation with \u03b2-cyclodextrin. Beilstein J Org Chem 12:179\u2013191. https:\/\/doi.org\/10.3762\/bjoc.12.20","journal-title":"Beilstein J Org Chem"},{"key":"12626_CR25","doi-asserted-by":"publisher","first-page":"119442","DOI":"10.1016\/j.fuel.2020.119442","volume":"287","author":"R Halim","year":"2021","unstructured":"Halim R, Papachristou I, Kubisch C, Nazarova N, W\u00fcstner R, Steinbach D, Chen GQ, Deng H, Frey W, Posten C, Silve A (2021) Hypotonic osmotic shock treatment to enhance lipid and protein recoveries from concentrated saltwater Nannochloropsis slurries. Fuel 287:119442. https:\/\/doi.org\/10.1016\/j.fuel.2020.119442","journal-title":"Fuel"},{"key":"12626_CR26","doi-asserted-by":"publisher","first-page":"420","DOI":"10.1016\/0003-2697(78)90046-5","volume":"90","author":"A Hara","year":"1978","unstructured":"Hara A, Radin NS (1978) Lipid extraction of tissues with a low toxicity solvent. Anal Biochem 90:420\u2013426. https:\/\/doi.org\/10.1016\/0003-2697(78)90046-5","journal-title":"Anal Biochem"},{"key":"12626_CR27","doi-asserted-by":"publisher","first-page":"211","DOI":"10.1016\/j.supflu.2014.09.006","volume":"96","author":"M Herrero","year":"2015","unstructured":"Herrero M, Ib\u00e1\u00f1ez E (2015) Green processes and sustainability: an overview on the extraction of high added-value products from seaweeds and microalgae. J Supercrit Fluids 96:211\u2013216. https:\/\/doi.org\/10.1016\/j.supflu.2014.09.006","journal-title":"J Supercrit Fluids"},{"key":"12626_CR28","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1016\/j.enconman.2014.08.038","volume":"88","author":"MA Islam","year":"2014","unstructured":"Islam MA, Brown RJ, O\u2019Hara I, Kent M, Heimann K (2014) Effect of temperature and moisture on high pressure lipid\/oil extraction from microalgae. Energy Convers Manag 88:307\u2013316. https:\/\/doi.org\/10.1016\/j.enconman.2014.08.038","journal-title":"Energy Convers Manag"},{"key":"12626_CR29","doi-asserted-by":"publisher","first-page":"1636","DOI":"10.1016\/j.procbio.2016.07.016","volume":"51","author":"DP Jaeschke","year":"2016","unstructured":"Jaeschke DP, Menegol T, Rech R, Mercali GD, Marczak LDF (2016) Carotenoid and lipid extraction from Heterochlorella luteoviridis using moderate electric field and ethanol. Process Biochem 51:1636\u20131643. https:\/\/doi.org\/10.1016\/j.procbio.2016.07.016","journal-title":"Process Biochem"},{"key":"12626_CR30","doi-asserted-by":"publisher","first-page":"1117","DOI":"10.1007\/s10811-020-02037-z","volume":"32","author":"MJ Jim\u00e9nez Callej\u00f3n","year":"2020","unstructured":"Jim\u00e9nez Callej\u00f3n MJ, Robles Medina A, Gonz\u00e1lez Moreno PA, Esteban Cerd\u00e1n L, OrtaGuill\u00e9n S, Molina Grima E (2020) Simultaneous extraction and fractionation of lipids from the microalga Nannochloropsis sp. for the production of EPA-rich polar lipid concentrates. J Appl Phycol 32:1117\u20131128. https:\/\/doi.org\/10.1007\/s10811-020-02037-z","journal-title":"J Appl Phycol"},{"key":"12626_CR31","doi-asserted-by":"publisher","unstructured":"Joannes C, Sipaut CS, Dayou J, Yasir SM, Mansa RF (2015) Review paper on cell membrane electroporation of microalgae using electric field treatment method for microalgae lipid extraction. IOP Conf Ser Mater Sci Eng 78. https:\/\/doi.org\/10.1088\/1757-899X\/78\/1\/012034","DOI":"10.1088\/1757-899X\/78\/1\/012034"},{"key":"12626_CR32","doi-asserted-by":"publisher","unstructured":"Joshi DR, Adhikari N (2019) An overview on common organic solvents and their toxicity. J Pharm Res Int 1\u201318. https:\/\/doi.org\/10.9734\/jpri\/2019\/v28i330203","DOI":"10.9734\/jpri\/2019\/v28i330203"},{"key":"12626_CR33","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1111\/jfpe.12896","volume":"42","author":"SA Khan","year":"2018","unstructured":"Khan SA, Aslam R, Makroo HA (2018) High pressure extraction and its application in the extraction of bio-active compounds: a review. J Food Process Eng 42:1\u201315. https:\/\/doi.org\/10.1111\/jfpe.12896","journal-title":"J Food Process Eng"},{"key":"12626_CR34","doi-asserted-by":"publisher","unstructured":"Kojima Y, Shimizu A (2022) Effect of high hydrostatic pressure treatment with room-temperature ionic liquid 1-ethyl-3-methylimidazolium acetate - dimethyl sulfoxide mixture on lipid extraction from Chlorella vulgaris. High Press Res 1\u201316. https:\/\/doi.org\/10.1080\/08957959.2022.2044032","DOI":"10.1080\/08957959.2022.2044032"},{"key":"12626_CR35","doi-asserted-by":"publisher","unstructured":"Ma XN, Chen TP, Yang B, Liu J, Chen F (2016) Lipid production from Nannochloropsis. Mar Drugs 14. https:\/\/doi.org\/10.3390\/md14040061","DOI":"10.3390\/md14040061"},{"key":"12626_CR36","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1016\/j.jenvman.2018.04.102","volume":"219","author":"A Monteiro","year":"2018","unstructured":"Monteiro A, Paquincha D, Martins F, Queir\u00f3s RP, Saraiva JA, \u0160varc-Gaji\u0107 J, Nasti\u0107 N, Delerue-Matos C, Carvalho AP (2018) Liquid by-products from fish canning industry as sustainable sources of \u03c93 lipids. J Environ Manage 219:9\u201317. https:\/\/doi.org\/10.1016\/j.jenvman.2018.04.102","journal-title":"J Environ Manage"},{"key":"12626_CR37","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1016\/j.jbiotec.2021.05.004","volume":"334","author":"F Pagels","year":"2021","unstructured":"Pagels F, Pereira RN, Amaro HM, Vasconcelos V, Guedes AC, Vicente AA (2021) Continuous pressurized extraction versus electric fields-assisted extraction of cyanobacterial pigments. J Biotechnol 334:35\u201342. https:\/\/doi.org\/10.1016\/j.jbiotec.2021.05.004","journal-title":"J Biotechnol"},{"key":"12626_CR38","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1016\/j.ifset.2014.11.002","volume":"27","author":"O Parniakov","year":"2015","unstructured":"Parniakov O, Barba FJ, Grimi N, Marchal L, Jubeau S, Lebovka N, Vorobiev E (2015) Pulsed electric field assisted extraction of nutritionally valuable compounds from microalgae Nannochloropsis spp. using the binary mixture of organic solvents and water. Innov Food Sci Emerg Technol 27:79\u201385. https:\/\/doi.org\/10.1016\/j.ifset.2014.11.002","journal-title":"Innov Food Sci Emerg Technol"},{"key":"12626_CR39","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3390\/md16010003","volume":"16","author":"E Peltomaa","year":"2018","unstructured":"Peltomaa E, Johnson MD, Taipale SJ (2018) Marine cryptophytes are great sources of EPA and DHA. Mar Drugs 16:1\u201311. https:\/\/doi.org\/10.3390\/md16010003","journal-title":"Mar Drugs"},{"key":"12626_CR40","doi-asserted-by":"publisher","first-page":"474","DOI":"10.1016\/j.biombioe.2012.10.019","volume":"47","author":"S Pieber","year":"2012","unstructured":"Pieber S, Schober S, Mittelbach M (2012) Pressurized fluid extraction of polyunsaturated fatty acids from the microalga Nannochloropsis oculata. Biomass Bioenerg 47:474\u2013482. https:\/\/doi.org\/10.1016\/j.biombioe.2012.10.019","journal-title":"Biomass Bioenerg"},{"key":"12626_CR41","doi-asserted-by":"publisher","first-page":"501","DOI":"10.1016\/j.seppur.2017.10.007","volume":"192","author":"J Pinela","year":"2018","unstructured":"Pinela J, Prieto MA, Barros L, Carvalho AM, Oliveira MBPP, Saraiva JA, Ferreira ICFR (2018) Cold extraction of phenolic compounds from watercress by high hydrostatic pressure: process modelling and optimization. Sep Purif Technol 192:501\u2013512. https:\/\/doi.org\/10.1016\/j.seppur.2017.10.007","journal-title":"Sep Purif Technol"},{"key":"12626_CR42","doi-asserted-by":"publisher","first-page":"80","DOI":"10.1016\/j.biortech.2015.12.012","volume":"203","author":"PR Postma","year":"2016","unstructured":"Postma PR, Pataro G, Capitoli M, Barbosa MJ, Wijffels RH, Eppink MHM, Olivieri G, Ferrari G (2016) Selective extraction of intracellular components from the microalga Chlorella vulgaris by combined pulsed electric field-temperature treatment. Bioresour Technol 203:80\u201388. https:\/\/doi.org\/10.1016\/j.biortech.2015.12.012","journal-title":"Bioresour Technol"},{"key":"12626_CR43","doi-asserted-by":"publisher","first-page":"288","DOI":"10.1039\/c5gc01008j","volume":"18","author":"D Prat","year":"2015","unstructured":"Prat D, Wells A, Hayler J, Sneddon H, McElroy CR, Abou-Shehada S, Dunn PJ (2015) CHEM21 selection guide of classical- and less classical-solvents. Green Chem 18:288\u2013296. https:\/\/doi.org\/10.1039\/c5gc01008j","journal-title":"Green Chem"},{"key":"12626_CR44","doi-asserted-by":"publisher","first-page":"583","DOI":"10.1016\/j.bcab.2019.01.017","volume":"17","author":"B Ramesh Kumar","year":"2019","unstructured":"Ramesh Kumar B, Deviram G, Mathimani T, Duc PA, Pugazhendhi A (2019) Microalgae as rich source of polyunsaturated fatty acids. Biocatal Agric Biotechnol 17:583\u2013588. https:\/\/doi.org\/10.1016\/j.bcab.2019.01.017","journal-title":"Biocatal Agric Biotechnol"},{"key":"12626_CR45","doi-asserted-by":"publisher","unstructured":"Samarasinghe N (2012) Effect of high pressure homogenization on aqueous phase solvent extraction of lipids from Nannochloris oculata microalgae. J Energy Nat Resour 1:1. https:\/\/doi.org\/10.11648\/j.jenr.20120101.11","DOI":"10.11648\/j.jenr.20120101.11"},{"key":"12626_CR46","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1016\/j.ifset.2005.06.006","volume":"7","author":"A Sequeira-Munoz","year":"2006","unstructured":"Sequeira-Munoz A, Chevalier D, LeBail A, Ramaswamy HS, Simpson BK (2006) Physicochemical changes induced in carp (Cyprinus carpio) fillets by high pressure processing at low temperature. Innov Food Sci Emerg Technol 7:13\u201318. https:\/\/doi.org\/10.1016\/j.ifset.2005.06.006","journal-title":"Innov Food Sci Emerg Technol"},{"key":"12626_CR47","unstructured":"Sneddon H (2017) Solvents \u2013 solvent guides, guides for chromatography, common reactions, work-ups etc. GSK. https:\/\/www.cersuschem.ufscar.br\/documentos\/sneddon-cersuschem-short-course-2nd-session"},{"key":"12626_CR48","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1093\/chromsci\/16.6.223","volume":"16","author":"LR Snyder","year":"1978","unstructured":"Snyder LR (1978) Classification off the solvent properties of common liquids. J Chromatogr Sci 16:223\u2013234. https:\/\/doi.org\/10.1093\/chromsci\/16.6.223","journal-title":"J Chromatogr Sci"},{"key":"12626_CR49","doi-asserted-by":"publisher","first-page":"4017","DOI":"10.1007\/s00253-022-11968-1","volume":"106","author":"S Sousa","year":"2022","unstructured":"Sousa S, Freitas AC, Gomes AM, Carvalho AP (2022) Modulated stress to balance Nannochloropsis oculata growth and eicosapentaenoic acid production. Appl Microbiol Biotechnol 106:4017\u20134027. https:\/\/doi.org\/10.1007\/s00253-022-11968-1","journal-title":"Appl Microbiol Biotechnol"},{"key":"12626_CR50","doi-asserted-by":"publisher","first-page":"325","DOI":"10.1080\/08957959.2018.1488974","volume":"38","author":"W Sun","year":"2018","unstructured":"Sun W, Li J, Ramaswamy HS, Yu Y, Wang C, Zhu S (2018) Adiabatic compression heating of selected organic solvents under high pressure processing. High Press Res 38:325\u2013336. https:\/\/doi.org\/10.1080\/08957959.2018.1488974","journal-title":"High Press Res"},{"key":"12626_CR51","doi-asserted-by":"publisher","first-page":"477","DOI":"10.1016\/j.biortech.2014.08.024","volume":"171","author":"CL Teo","year":"2014","unstructured":"Teo CL, Idris A (2014) Enhancing the various solvent extraction method via microwave irradiation for extraction of lipids from marine microalgae in biodiesel production. Bioresour Technol 171:477\u2013481. https:\/\/doi.org\/10.1016\/j.biortech.2014.08.024","journal-title":"Bioresour Technol"},{"key":"12626_CR52","doi-asserted-by":"publisher","first-page":"182","DOI":"10.1016\/j.algal.2018.03.007","volume":"32","author":"A Udayan","year":"2018","unstructured":"Udayan A, Kathiresan S, Arumugam M (2018) Kinetin and gibberellic acid (GA3) act synergistically to produce high value polyunsaturated fatty acids in Nannochloropsis oceanica CASA CC201. Algal Res 32:182\u2013192. https:\/\/doi.org\/10.1016\/j.algal.2018.03.007","journal-title":"Algal Res"},{"key":"12626_CR53","doi-asserted-by":"publisher","first-page":"5723","DOI":"10.1039\/c7gc02735d","volume":"19","author":"WMA Wan Mahmood","year":"2017","unstructured":"Wan Mahmood WMA, Theodoropoulos C, Gonzalez-Miquel M (2017) Enhanced microalgal lipid extraction using bio-based solvents for sustainable biofuel production. Green Chem 19:5723\u20135733. https:\/\/doi.org\/10.1039\/c7gc02735d","journal-title":"Green Chem"},{"key":"12626_CR54","doi-asserted-by":"publisher","first-page":"102412","DOI":"10.1016\/j.algal.2021.102412","volume":"58","author":"J Xu","year":"2021","unstructured":"Xu J, Zhao F, Su X (2021) Direct extraction of lipids from wet microalgae slurries by super-high hydrostatic pressure. Algal Res 58:102412. https:\/\/doi.org\/10.1016\/j.algal.2021.102412","journal-title":"Algal Res"},{"key":"12626_CR55","doi-asserted-by":"publisher","unstructured":"Zhao Y, Jiang Y, Ding Y, Wang D, Deng Y (2019) High hydrostatic pressure-assisted extraction of high-molecular-weight melanoidins from black garlic: composition, structure, and bioactive properties. J Food Qual 2019. https:\/\/doi.org\/10.1155\/2019\/1682749","DOI":"10.1155\/2019\/1682749"}],"container-title":["Applied Microbiology and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-023-12626-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00253-023-12626-w\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-023-12626-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,29]],"date-time":"2023-07-29T11:04:31Z","timestamp":1690628671000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00253-023-12626-w"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,29]]},"references-count":55,"journal-issue":{"issue":"16","published-print":{"date-parts":[[2023,8]]}},"alternative-id":["12626"],"URL":"https:\/\/doi.org\/10.1007\/s00253-023-12626-w","relation":{},"ISSN":["0175-7598","1432-0614"],"issn-type":[{"value":"0175-7598","type":"print"},{"value":"1432-0614","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,29]]},"assertion":[{"value":"12 January 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 April 2023","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 June 2023","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 June 2023","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This article does not contain any studies with human participants or animals performed by any of the authors.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}