{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T13:40:27Z","timestamp":1776087627207,"version":"3.50.1"},"reference-count":72,"publisher":"Springer Science and Business Media LLC","issue":"16","license":[{"start":{"date-parts":[[2023,7,5]],"date-time":"2023-07-05T00:00:00Z","timestamp":1688515200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2023,7,5]],"date-time":"2023-07-05T00:00:00Z","timestamp":1688515200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PTDC\/EAM-AMB\/30488\/2017"],"award-info":[{"award-number":["PTDC\/EAM-AMB\/30488\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["POCI-01-0145-FEDER-007569"],"award-info":[{"award-number":["POCI-01-0145-FEDER-007569"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["POCI-01-0145-FEDER-022184"],"award-info":[{"award-number":["POCI-01-0145-FEDER-022184"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BD\/76894\/2011"],"award-info":[{"award-number":["SFRH\/BD\/76894\/2011"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PD\/BD\/146184\/2019"],"award-info":[{"award-number":["PD\/BD\/146184\/2019"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008814","name":"Universidade do Minho","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100008814","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Appl Microbiol Biotechnol"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:sec>\n                <jats:title>Abstract<\/jats:title>\n                <jats:p>The biocatalysis of \u03b2-myrcene into value-added compounds, with enhanced organoleptic\/therapeutic properties, may be performed by resorting to specialized enzymatic machinery of \u03b2-myrcene-biotransforming bacteria. Few \u03b2-myrcene-biotransforming bacteria have been studied, limiting the diversity of genetic modules\/catabolic pathways available for biotechnological research. In our model <jats:italic>Pseudomonas<\/jats:italic> sp. strain M1, the \u03b2-myrcene catabolic core-code was identified in a 28-kb genomic island (GI). The lack of close homologs of this \u03b2-myrcene-associated genetic code prompted a bioprospection of cork oak and eucalyptus rhizospheres, from 4 geographic locations in Portugal, to evaluate the environmental diversity and dissemination of the \u03b2-myrcene-biotransforming genetic trait (Myr<jats:sup>+<\/jats:sup>).<\/jats:p>\n                <jats:p>Soil microbiomes were enriched in \u03b2-myrcene-supplemented cultures, from which \u03b2-myrcene-biotransforming bacteria were isolated, belonging to <jats:italic>Alphaproteobacteria<\/jats:italic>, <jats:italic>Betaproteobacteria<\/jats:italic>, <jats:italic>Gammaproteobacteria<\/jats:italic>, and <jats:italic>Sphingobacteriia<\/jats:italic> classes. From a panel of representative Myr<jats:sup>+<\/jats:sup> isolates that included 7 bacterial genera, the production of \u03b2-myrcene derivatives previously reported in strain M1 was detected in <jats:italic>Pseudomonas<\/jats:italic> spp., <jats:italic>Cupriavidus<\/jats:italic> sp., <jats:italic>Sphingobacterium<\/jats:italic> sp., and <jats:italic>Variovorax<\/jats:italic> sp. A comparative genomics analysis against the genome of strain M1 found the M1-GI code in 11 new <jats:italic>Pseudomonas<\/jats:italic> genomes. Full nucleotide conservation of the \u03b2-myrcene core-code was observed throughout a 76-kb <jats:italic>locus<\/jats:italic> in strain M1 and all 11 <jats:italic>Pseudomonas<\/jats:italic> spp., resembling the structure of an integrative and conjugative element (ICE), despite being isolated from different niches.<\/jats:p>\n                <jats:p>Furthermore, the characterization of isolates not harboring the Myr<jats:sup>+<\/jats:sup>-related 76-kb <jats:italic>locus<\/jats:italic> suggested that they may biotransform \u03b2-myrcene via alternative catabolic loci, being thereby a novel source of enzymes and biomolecule catalogue for biotechnological exploitation.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Key points<\/jats:title>\n                <jats:p>\u2022 <jats:italic>The isolation of 150 Myr<\/jats:italic><jats:sup>+<\/jats:sup><jats:italic>bacteria hints the ubiquity of such trait in the rhizosphere.<\/jats:italic><\/jats:p>\n                <jats:p>\u2022 <jats:italic>The Myr<\/jats:italic><jats:sup>+<\/jats:sup><jats:italic>trait is spread across different bacterial taxonomic classes.<\/jats:italic><\/jats:p>\n                <jats:p>\u2022 <jats:italic>The core-code for the Myr<\/jats:italic><jats:sup>+<\/jats:sup><jats:italic>trait was detected in a novel ICE, only found in Pseudomonas spp.<\/jats:italic><\/jats:p>\n              <\/jats:sec>","DOI":"10.1007\/s00253-023-12650-w","type":"journal-article","created":{"date-parts":[[2023,7,5]],"date-time":"2023-07-05T10:04:36Z","timestamp":1688551476000},"page":"5209-5224","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Bioprospection of the bacterial \u03b2-myrcene-biotransforming trait in the rhizosphere"],"prefix":"10.1007","volume":"107","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0578-0299","authenticated-orcid":false,"given":"Pedro","family":"Soares-Castro","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4800-9042","authenticated-orcid":false,"given":"Filipa","family":"Soares","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0873-061X","authenticated-orcid":false,"given":"Francisca","family":"Reis","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1385-4799","authenticated-orcid":false,"given":"Teresa","family":"Lino-Neto","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3234-4265","authenticated-orcid":false,"given":"Pedro M.","family":"Santos","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2023,7,5]]},"reference":[{"key":"12650_CR1","doi-asserted-by":"publisher","first-page":"808","DOI":"10.1007\/s10886-011-9992-6","volume":"37","author":"AS Adams","year":"2011","unstructured":"Adams AS, Boone CK, Bohlmann J, Raffa KF (2011) Responses of bark beetle-associated bacteria to host monoterpenes and their relationship to insect life histories. J Chem Ecol 37:808\u2013817. https:\/\/doi.org\/10.1007\/s10886-011-9992-6","journal-title":"J Chem Ecol"},{"key":"12650_CR2","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1089\/omi.2008.0017","volume":"12","author":"SV Angiuoli","year":"2008","unstructured":"Angiuoli SV, Gussman A, Klimke W, Cochrane G, Field D, Garrity GM, Kodira CD, Kyrpides N, Madupu R, Markowitz V, Tatusova T, Thomson N, White O (2008) Toward an online repository of standard operating procedures (SOPs) for (meta) genomic annotation. OMICS 12:137\u2013141. https:\/\/doi.org\/10.1089\/omi.2008.0017","journal-title":"OMICS"},{"key":"12650_CR3","unstructured":"Aronesty E (2011) ea-utils: command-line tools for processing biological sequencing data. Available at https:\/\/github.com\/ExpressionAnalysis\/ea-utils"},{"key":"12650_CR4","doi-asserted-by":"publisher","first-page":"1072","DOI":"10.1002\/cssc.200900186","volume":"2","author":"A Behr","year":"2009","unstructured":"Behr A, Johnen L (2009) Myrcene as a natural base chemical in sustainable chemistry: a critical review. Chemsuschem 2:1072\u20131095. https:\/\/doi.org\/10.1002\/cssc.200900186","journal-title":"Chemsuschem"},{"key":"12650_CR5","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1007\/s00253-009-2234-y","volume":"86","author":"SG Bell","year":"2010","unstructured":"Bell SG, Dale A, Rees NH, Wong L-L (2010) A cytochrome P450 class I electron transfer system from Novosphingobium aromaticivorans. Appl Microbiol Biotechnol 86:163\u2013175. https:\/\/doi.org\/10.1007\/s00253-009-2234-y","journal-title":"Appl Microbiol Biotechnol"},{"key":"12650_CR6","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1186\/s12864-015-1632-z","volume":"16","author":"RL Berendsen","year":"2015","unstructured":"Berendsen RL, van Verk MC, Stringlis IA, Zamioudis C, Tommassen J, Pieterse CMJ, Bakker PAHM (2015) Unearthing the genomes of plant-beneficial Pseudomonas model strains WCS358, WCS374 and WCS417. BMC Genomics 16:539. https:\/\/doi.org\/10.1186\/s12864-015-1632-z","journal-title":"BMC Genomics"},{"key":"12650_CR7","doi-asserted-by":"publisher","first-page":"e105515","DOI":"10.1371\/journal.pone.0105515","volume":"9","author":"A Bevivino","year":"2014","unstructured":"Bevivino A, Paganin P, Bacci G, Florio A, Pellicer MS, Papaleo MC, Mengoni A, Ledda L, Fani R, Benedetti A (2014) Soil bacterial community response to differences in agricultural management along with seasonal changes in a Mediterranean region. PLoS One 9:e105515. https:\/\/doi.org\/10.1371\/journal.pone.0105515","journal-title":"PLoS One"},{"issue":"Database issue","key":"12650_CR8","doi-asserted-by":"publisher","first-page":"D621","DOI":"10.1093\/nar\/gkr846","volume":"40","author":"D Bi","year":"2011","unstructured":"Bi D, Xu Z, Harrison EM, Tai C, Wei Y, He X, Jia S, Deng Z, Rajakumar K, Ou H-Y (2011) ICEberg: a web-based resource for integrative and conjugative elements found in Bacteria. Nucleic Acids Res 40(Database issue):D621-6. https:\/\/doi.org\/10.1093\/nar\/gkr846","journal-title":"Nucleic Acids Res"},{"key":"12650_CR9","doi-asserted-by":"publisher","first-page":"4518","DOI":"10.1021\/cr800190y","volume":"109","author":"JL Bicas","year":"2009","unstructured":"Bicas JL, Dion\u00edsio AP, Pastore GM (2009) Bio-oxidation of terpenes: an approach for the flavor industry. Chem Rev 109:4518\u20134531. https:\/\/doi.org\/10.1021\/cr800190y","journal-title":"Chem Rev"},{"key":"12650_CR10","doi-asserted-by":"publisher","first-page":"1589","DOI":"10.1046\/j.1365-2958.2002.02981.x","volume":"44","author":"EM Camacho","year":"2002","unstructured":"Camacho EM, Casades\u00fas J (2002) Conjugal transfer of the virulence plasmid of Salmonella enterica is regulated by the leucine-responsive regulatory protein and DNA adenine methylation. Mol Microbiol 44:1589\u20131598. https:\/\/doi.org\/10.1046\/j.1365-2958.2002.02981.x","journal-title":"Mol Microbiol"},{"key":"12650_CR11","doi-asserted-by":"publisher","first-page":"830","DOI":"10.1128\/MMBR.00016-06","volume":"70","author":"J Casades\u00fas","year":"2006","unstructured":"Casades\u00fas J, Low D (2006) Epigenetic gene regulation in the bacterial world. Microbiol Mol Biol Rev 70:830\u2013856. https:\/\/doi.org\/10.1128\/MMBR.00016-06","journal-title":"Microbiol Mol Biol Rev"},{"key":"12650_CR12","doi-asserted-by":"publisher","first-page":"1869","DOI":"10.1038\/srep01869","volume":"3","author":"X-Y Cheng","year":"2013","unstructured":"Cheng X-Y, Tian X-L, Wang Y-S, Lin R-M, Mao Z-C, Chen N, Xie B-Y (2013) Metagenomic analysis of the pinewood nematode microbiome reveals a symbiotic relationship critical for xenobiotics degradation. Sci Rep 3:1869. https:\/\/doi.org\/10.1038\/srep01869","journal-title":"Sci Rep"},{"key":"12650_CR13","doi-asserted-by":"publisher","first-page":"903","DOI":"10.1385\/abab:84-86:1-9:903","volume":"84\u201386","author":"TK Cheong","year":"2000","unstructured":"Cheong TK, Oriel PJ (2000) Cloning and expression of the limonene hydroxylase of Bacillus stearothermophilus BR388 and utilization in two-phase limonene conversions. Appl Biochem Biotechnol 84\u201386:903\u2013915. https:\/\/doi.org\/10.1385\/abab:84-86:1-9:903","journal-title":"Appl Biochem Biotechnol"},{"issue":"Database issue","key":"12650_CR14","doi-asserted-by":"publisher","first-page":"D633","DOI":"10.1093\/nar\/gkt1244","volume":"42","author":"JR Cole","year":"2013","unstructured":"Cole JR, Wang Q, Fish JA, Chai B, McGarrell DM, Sun Y, Brown CT, Porras-Alfaro A, Kuske CR, Tiedje JM (2013) Ribosomal database project: data and tools for high throughput rRNA analysis. Nucleic Acids Res 42(Database issue):D633-42. https:\/\/doi.org\/10.1093\/nar\/gkt1244","journal-title":"Nucleic Acids Res"},{"key":"12650_CR15","doi-asserted-by":"publisher","first-page":"183","DOI":"10.1007\/s00248-017-1014-y","volume":"75","author":"FE da Silva","year":"2018","unstructured":"da Silva FE, Peixoto RS, Rosado AS, de Carvalho BF, Tiedje JM, da Costa Rachid CTC (2018) The microbiome of Eucalyptus roots under different management conditions and its potential for biological nitrogen fixation. Microb Ecol 75:183\u2013191. https:\/\/doi.org\/10.1007\/s00248-017-1014-y","journal-title":"Microb Ecol"},{"key":"12650_CR16","doi-asserted-by":"publisher","first-page":"3171","DOI":"10.1128\/jb.179.10.3171-3180.1997","volume":"179","author":"RW Eaton","year":"1996","unstructured":"Eaton RW (1996) p-Cumate catabolic pathway in Pseudomonas putida Fl: cloning and characterization of DNA carrying the cmt operon. J Bacteriol 179:3171\u20133180. https:\/\/doi.org\/10.1128\/jb.179.10.3171-3180.1997","journal-title":"J Bacteriol"},{"key":"12650_CR17","doi-asserted-by":"publisher","first-page":"1351","DOI":"10.1128\/jb.178.5.1351-1362.1996","volume":"178","author":"RW Eaton","year":"1997","unstructured":"Eaton RW (1997) p-Cymene catabolic pathway in Pseudomonas putida F1: cloning and characterization of DNA encoding conversion of p-cymene to p-cumate. J Bacteriol 178:1351\u20131362. https:\/\/doi.org\/10.1128\/jb.178.5.1351-1362.1996","journal-title":"J Bacteriol"},{"key":"12650_CR18","doi-asserted-by":"publisher","first-page":"308","DOI":"10.1002\/ptr.2072","volume":"21","author":"AE Edris","year":"2007","unstructured":"Edris AE (2007) Pharmaceutical and therapeutic potentials of essential oils and their individual volatile constituents: a review. Phyther Res 21:308\u2013323. https:\/\/doi.org\/10.1002\/ptr.2072","journal-title":"Phyther Res"},{"key":"12650_CR19","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1186\/1752-153X-5-26","volume":"5","author":"A Esmaeili","year":"2011","unstructured":"Esmaeili A, Hashemi E (2011) Biotransformation of myrcene by Pseudomonas aeruginosa. Chem Cent J 5:26. https:\/\/doi.org\/10.1186\/1752-153X-5-26","journal-title":"Chem Cent J"},{"key":"12650_CR20","doi-asserted-by":"publisher","first-page":"186","DOI":"10.1101\/gr.8.3.175","volume":"8","author":"B Ewing","year":"1998","unstructured":"Ewing B, Green P (1998) Base-calling of automated sequencer traces using Phred. II. Error probabilities. Genome Res 8:186\u2013194. https:\/\/doi.org\/10.1101\/gr.8.3.175","journal-title":"Genome Res"},{"key":"12650_CR21","doi-asserted-by":"publisher","first-page":"4819","DOI":"10.1128\/AEM.00853-06","volume":"72","author":"K F\u00f6rster-Fromme","year":"2006","unstructured":"F\u00f6rster-Fromme K, H\u00f6schle B, Mack C, Bott M, Armbruster W, Jendrossek D (2006) Identification of genes and proteins necessary for catabolism of acyclic terpenes and leucine\/isovalerate in Pseudomonas aeruginosa. Appl Environ Microbiol 72:4819\u20134828. https:\/\/doi.org\/10.1128\/AEM.00853-06","journal-title":"Appl Environ Microbiol"},{"key":"12650_CR22","doi-asserted-by":"publisher","first-page":"1939","DOI":"10.1128\/aem.66.5.1939-1946.2000","volume":"66","author":"BBM Gardener","year":"2000","unstructured":"Gardener BBM, Schroeder KL, Kalloger SE, Raaijmakers JM, Thomashow LS, Weller DM (2000) Genotypic and phenotypic diversity of phlD-containing Pseudomonas strains isolated from the rhizosphere of wheat. Appl Environ Microbiol 66:1939\u20131946. https:\/\/doi.org\/10.1128\/aem.66.5.1939-1946.2000","journal-title":"Appl Environ Microbiol"},{"key":"12650_CR23","doi-asserted-by":"publisher","first-page":"2723","DOI":"10.1093\/bioinformatics\/btu368","volume":"30","author":"P Greenfield","year":"2014","unstructured":"Greenfield P, Duesing K, Papanicolaou A, Bauer DC (2014) Blue: correcting sequencing errors using consensus and context. Bioinformatics 30:2723\u20132732. https:\/\/doi.org\/10.1093\/bioinformatics\/btu368","journal-title":"Bioinformatics"},{"key":"12650_CR24","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1093\/sysbio\/syq010","volume":"59","author":"S Guindon","year":"2010","unstructured":"Guindon S, Dufayard J-F, Lefort V, Anisimova M, Hordijk W, Gascuel O (2010) New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Syst Biol 59:307\u2013321. https:\/\/doi.org\/10.1093\/sysbio\/syq010","journal-title":"Syst Biol"},{"key":"12650_CR25","doi-asserted-by":"publisher","first-page":"27725","DOI":"10.1074\/jbc.M203382200","volume":"277","author":"DB Hawkes","year":"2002","unstructured":"Hawkes DB, Adams GW, Burlingame AL, de Montellano PRO, De Voss JJ (2002) Cytochrome P450cin (CYP176A), isolation, expression, and characterization. J Biol Chem 277:27725\u201327732. https:\/\/doi.org\/10.1074\/jbc.M203382200","journal-title":"J Biol Chem"},{"key":"12650_CR26","doi-asserted-by":"publisher","first-page":"2871","DOI":"10.1128\/AEM.65.7.2871-2876.1999","volume":"65","author":"S Iurescia","year":"1999","unstructured":"Iurescia S, Marconi AM, Tofani D, Gambacorta A, Patern\u00f2 A, Devirgiliis C, van der Werf MJ, Zennaro E (1999) Identification and sequencing of \u03b2-myrcene catabolism genes from Pseudomonas sp. strain M1. Appl Environ Microbiol 65:2871\u20132876","journal-title":"Appl Environ Microbiol"},{"key":"12650_CR27","doi-asserted-by":"publisher","first-page":"5114","DOI":"10.1038\/s41467-018-07641-9","volume":"9","author":"C Jain","year":"2018","unstructured":"Jain C, Rodriguez-R LM, Phillippy AM, Konstantinidis KT, Aluru S (2018) High throughput ANI analysis of 90K prokaryotic genomes reveals clear species boundaries. Nat Commun 9:5114. https:\/\/doi.org\/10.1038\/s41467-018-07641-9","journal-title":"Nat Commun"},{"key":"12650_CR28","doi-asserted-by":"publisher","first-page":"772","DOI":"10.1093\/molbev\/mst010","volume":"30","author":"K Katoh","year":"2013","unstructured":"Katoh K, Standley DM (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol 30:772\u2013780. https:\/\/doi.org\/10.1093\/molbev\/mst010","journal-title":"Mol Biol Evol"},{"key":"12650_CR29","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1002\/em.2850180106","volume":"18","author":"B Kauderer","year":"1991","unstructured":"Kauderer B, Zamith H, Paumgartten FJR, Speit G, Holden HE (1991) Evaluation of the mutagenicity of \u03b2-myrcene in mammalian cells in vitro. Environ Mol Mutagen 18:28\u201334. https:\/\/doi.org\/10.1002\/em.2850180106","journal-title":"Environ Mol Mutagen"},{"key":"12650_CR30","doi-asserted-by":"publisher","first-page":"2068","DOI":"10.1128\/jb.176.7.2068-2081.1994","volume":"176","author":"JE Kirby","year":"1994","unstructured":"Kirby JE, Trempy JE, Gottesman S (1994) Excision of a P4-like cryptic prophage leads to Alp protease expression in Escherichia coli. J Bacteriol 176:2068\u20132081. https:\/\/doi.org\/10.1128\/jb.176.7.2068-2081.1994","journal-title":"J Bacteriol"},{"key":"12650_CR31","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s002530051129","volume":"49","author":"U Krings","year":"1998","unstructured":"Krings U, Berger GR (1998) Biotechnological production of flavours and fragrances. Appl Microbiol Biotechnol 49:1\u20138. https:\/\/doi.org\/10.1007\/s002530051129","journal-title":"Appl Microbiol Biotechnol"},{"key":"12650_CR32","doi-asserted-by":"publisher","first-page":"114","DOI":"10.5808\/GI.2013.11.3.114","volume":"11","author":"MH Lee","year":"2013","unstructured":"Lee MH, Lee SW (2013) Bioprospecting potential of the soil metagenome: novel enzymes and bioactivities. Genom Inform 11:114\u2013120. https:\/\/doi.org\/10.5808\/GI.2013.11.3.114","journal-title":"Genom Inform"},{"key":"12650_CR33","doi-asserted-by":"publisher","first-page":"20471","DOI":"10.1016\/S0021-9258(19)88726-4","volume":"267","author":"M Lessl","year":"1992","unstructured":"Lessl M, Balzer D, Pansegrau W, Lanka E (1992) Sequence similarities between the RP4 Tra2 and the Ti VirB region strongly support the conjugation model for T-DNA transfer. J Biol Chem 267:20471\u201320480. https:\/\/doi.org\/10.1016\/S0021-9258(19)88726-4","journal-title":"J Biol Chem"},{"key":"12650_CR34","doi-asserted-by":"publisher","first-page":"951","DOI":"10.1016\/j.soilbio.2006.11.007","volume":"39","author":"C Lin","year":"2007","unstructured":"Lin C, Owen SM, Pe\u00f1uelas J (2007) Volatile organic compounds in the roots and rhizosphere of Pinus spp. Soil Biol Biochem 39:951\u2013960. https:\/\/doi.org\/10.1016\/j.soilbio.2006.11.007","journal-title":"Soil Biol Biochem"},{"key":"12650_CR35","doi-asserted-by":"publisher","first-page":"409","DOI":"10.1515\/znc-2011-7-813","volume":"66","author":"F L\u00fcddeke","year":"2011","unstructured":"L\u00fcddeke F, Harder J (2011) Enantiospecific (S)-(+)-linalool formation from beta-myrcene by linalool dehydratase-isomerase. Z Naturforsch C 66:409\u2013412. https:\/\/doi.org\/10.1515\/znc-2011-7-813","journal-title":"Z Naturforsch C"},{"key":"12650_CR36","doi-asserted-by":"publisher","first-page":"2128","DOI":"10.1128\/AEM.07226-11","volume":"78","author":"F L\u00fcddeke","year":"2012","unstructured":"L\u00fcddeke F, W\u00fclfing A, Timke M, Germer F, Weber J, Dikfidan A, Rahnfeld T, Linder D, Meyerdierks A, Harder J (2012) Geraniol and geranial dehydrogenases induced in anaerobic monoterpene degradation by Castellaniella defragrans. Appl Environ Microbiol 78:2128\u20132136. https:\/\/doi.org\/10.1128\/AEM.07226-11","journal-title":"Appl Environ Microbiol"},{"key":"12650_CR37","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1016\/0027-5107(75)90309-7","volume":"28","author":"MG Marinus","year":"1975","unstructured":"Marinus MG, Morris NR (1975) Pleiotropic effects of a DNA adenine methylation mutation (dam-3) in Escherichia coli K12. Mutat Res Mol Mech Mutagen 28:15\u201326. https:\/\/doi.org\/10.1016\/0027-5107(75)90309-7","journal-title":"Mutat Res Mol Mech Mutagen"},{"key":"12650_CR38","doi-asserted-by":"publisher","first-page":"2309","DOI":"10.1021\/jf051682i","volume":"54","author":"R Matsuura","year":"2006","unstructured":"Matsuura R, Ukeda H, Sawamura M (2006) Tyrosinase inhibitory activity of citrus essential oils. J Agric Food Chem 54:2309\u20132313. https:\/\/doi.org\/10.1021\/jf051682i","journal-title":"J Agric Food Chem"},{"key":"12650_CR39","doi-asserted-by":"publisher","first-page":"20792","DOI":"10.1073\/pnas.0806164106","volume":"105","author":"M Minoia","year":"2008","unstructured":"Minoia M, Gaillard M, Reinhard F, Stojanov M, Sentchilo V, van der Meer JR (2008) Stochasticity and bistability in horizontal transfer control of a genomic island in Pseudomonas. Proc Natl Acad Sci 105:20792\u201320797. https:\/\/doi.org\/10.1073\/pnas.0806164106","journal-title":"Proc Natl Acad Sci"},{"key":"12650_CR40","doi-asserted-by":"publisher","first-page":"572","DOI":"10.1007\/s002530050975","volume":"47","author":"G Misra","year":"1997","unstructured":"Misra G, Pavlostathis SG (1997) Biodegradation kinetics of monoterpenes in liquid and soil-slurry systems. Appl Microbiol Biotechnol 47:572\u2013577. https:\/\/doi.org\/10.1007\/s002530050975","journal-title":"Appl Microbiol Biotechnol"},{"key":"12650_CR41","doi-asserted-by":"publisher","first-page":"e1002818","DOI":"10.1371\/journal.pgen.1002818","volume":"8","author":"R Miyazaki","year":"2012","unstructured":"Miyazaki R, Minoia M, Pradervand N, Sulser S, Reinhard F, van der Meer JR (2012) Cellular variability of RpoS expression underlies subpopulation activation of an integrative and conjugative element. PLoS Genet 8:e1002818. https:\/\/doi.org\/10.1371\/journal.pgen.1002818","journal-title":"PLoS Genet"},{"key":"12650_CR42","first-page":"525","volume-title":"Advances in Biotechnology","author":"H Narushima","year":"1981","unstructured":"Narushima H, Omori T, Minoda Y (1981) Microbial oxidation of \u03b2-myrcene. In: Vezina C, Singh K (eds) Advances in Biotechnology. Pergamon Press, Oxford, pp 525\u2013531"},{"key":"12650_CR43","doi-asserted-by":"publisher","first-page":"16122","DOI":"10.1016\/S0021-9258(17)36209-9","volume":"260","author":"TL Poulos","year":"1985","unstructured":"Poulos TL, Finzel BC, Gunsalus IC, Wagner GC, Kraut J (1985) The 2.6-A crystal structure of Pseudomonas putida cytochrome P-450. J Biol Chem 260:16122\u201316130. https:\/\/doi.org\/10.1016\/S0021-9258(17)36209-9","journal-title":"J Biol Chem"},{"key":"12650_CR44","unstructured":"R Core Team (2013) R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. Available online at https:\/\/www.R-project.org\/.\u00a0 Accessed\u00a0Feb 2023"},{"key":"12650_CR45","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1016\/j.apsoil.2019.05.031","volume":"143","author":"F Reis","year":"2019","unstructured":"Reis F, Soares-Castro P, Costa D, Tavares RM, Baptista P, Santos PM, Lino-Neto T (2019) Climatic impacts on the bacterial community profiles of cork oak soils. Appl Soil Ecol 143:89\u201397. https:\/\/doi.org\/10.1016\/j.apsoil.2019.05.031","journal-title":"Appl Soil Ecol"},{"key":"12650_CR46","doi-asserted-by":"publisher","first-page":"96","DOI":"10.1080\/10425170310001656765","volume":"15","author":"GA Roberts","year":"2004","unstructured":"Roberts GA, Grogan G, Turner NJ, Flitsch SL (2004) Nucleotide sequence of a portion of the camphor-degrading gene cluster from Rhodococcus sp. NCIMB 9784. DNA Seq 15:96\u2013103. https:\/\/doi.org\/10.1080\/10425170310001656765","journal-title":"DNA Seq"},{"key":"12650_CR47","doi-asserted-by":"publisher","first-page":"6028","DOI":"10.1128\/jb.175.18.6028-6037.1993","volume":"175","author":"JD Ropp","year":"1993","unstructured":"Ropp JD, Gunsalus IC, Sligar SG (1993) Cloning and expression of a member of a new cytochrome P-450 family: cytochrome P-450lin (CYP111) from Pseudomonas incognita. J Bacteriol 175:6028\u20136037. https:\/\/doi.org\/10.1128\/jb.175.18.6028-6037.1993","journal-title":"J Bacteriol"},{"key":"12650_CR48","doi-asserted-by":"publisher","first-page":"5101","DOI":"10.1002\/pmic.200900325","volume":"9","author":"PM Santos","year":"2009","unstructured":"Santos PM, S\u00e1-Correia I (2009) Adaptation to \u03b2-myrcene catabolism in Pseudomonas sp. M1: An expression proteomics analysis. Proteomics 9:5101\u20135111. https:\/\/doi.org\/10.1002\/pmic.200900325","journal-title":"Proteomics"},{"key":"12650_CR49","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1002\/ejlt.201200157","volume":"115","author":"W Schwab","year":"2013","unstructured":"Schwab W, Fuchs C, Huang F-C (2013) Transformation of terpenes into fine chemicals. Eur J Lipid Sci Technol 115:3\u20138. https:\/\/doi.org\/10.1002\/ejlt.201200157","journal-title":"Eur J Lipid Sci Technol"},{"key":"12650_CR50","doi-asserted-by":"publisher","first-page":"3436","DOI":"10.1016\/j.soilbio.2006.05.019","volume":"38","author":"A Smolander","year":"2006","unstructured":"Smolander A, Ketola RA, Kotiaho T, Kanerva S, Suominen K, Kitunen V (2006) Volatile monoterpenes in soil atmosphere under birch and conifers: effects on soil N transformations. Soil Biol Biochem 38:3436\u20133442. https:\/\/doi.org\/10.1016\/j.soilbio.2006.05.019","journal-title":"Soil Biol Biochem"},{"key":"12650_CR51","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1093\/gbe\/evu254","volume":"7","author":"P Soares-Castro","year":"2015","unstructured":"Soares-Castro P, Santos PM (2015) Deciphering the genome repertoire of Pseudomonas sp. M1 toward \u03b2-myrcene biotransformation. Genome Biol Evol 7:1\u201317. https:\/\/doi.org\/10.1093\/gbe\/evu254","journal-title":"Genome Biol Evol"},{"key":"12650_CR52","doi-asserted-by":"publisher","first-page":"e03112","DOI":"10.1128\/AEM.03112-16","volume":"83","author":"P Soares-Castro","year":"2017","unstructured":"Soares-Castro P, Montenegro-Silva P, Heipieper HJ, Santos PM (2017) Functional characterization of a 28-kilobase catabolic island from Pseudomonas sp. strain M1 involved in biotransformation of \u03b2-myrcene and related plant-derived volatiles. Appl Environ Microbiol 83:e03112-e3116. https:\/\/doi.org\/10.1128\/AEM.03112-16","journal-title":"Appl Environ Microbiol"},{"key":"12650_CR53","doi-asserted-by":"publisher","first-page":"91","DOI":"10.3390\/molecules26010091","volume":"26","author":"P Soares-Castro","year":"2021","unstructured":"Soares-Castro P, Soares F, Santos PM (2021) Current advances in the bacterial toolbox for the biotechnological production of monoterpene-based aroma compounds. Molecules 26:91. https:\/\/doi.org\/10.3390\/molecules26010091","journal-title":"Molecules"},{"key":"12650_CR54","doi-asserted-by":"publisher","first-page":"62","DOI":"10.1016\/j.compbiolchem.2012.01.001","volume":"36","author":"L Song","year":"2012","unstructured":"Song L, Pan Y, Chen S, Zhang X (2012) Structural characteristics of genomic islands associated with GMP synthases as integration hotspot among sequenced microbial genomes. Comput Biol Chem 36:62\u201370. https:\/\/doi.org\/10.1016\/j.compbiolchem.2012.01.001","journal-title":"Comput Biol Chem"},{"key":"12650_CR55","doi-asserted-by":"publisher","first-page":"42","DOI":"10.1128\/AEM.67.1.42-50.2001","volume":"67","author":"D Springael","year":"2001","unstructured":"Springael D, Ryngaert A, Merlin C, Toussaint A, Mergeay M (2001) Occurrence of Tn4371-related mobile elements and sequences in (chloro) biphenyl-degrading bacteria. Appl Environ Microbiol 67:42\u201350. https:\/\/doi.org\/10.1128\/AEM.67.1.42-50.2001","journal-title":"Appl Environ Microbiol"},{"key":"12650_CR56","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1146\/annurev.mi.45.100191.000513","volume":"45","author":"DB Steele","year":"1991","unstructured":"Steele DB, Stowers MD (1991) Techniques for selection of industrially important microorganisms. Annu Rev Microbiol 45:89\u2013106. https:\/\/doi.org\/10.1146\/annurev.mi.45.100191.000513","journal-title":"Annu Rev Microbiol"},{"key":"12650_CR57","doi-asserted-by":"publisher","first-page":"770","DOI":"10.1016\/S1044-0305(99)00047-1","volume":"10","author":"SE Stein","year":"1999","unstructured":"Stein SE (1999) An integrated method for spectrum extraction and compound identification from gas chromatography\/mass spectrometry data. J Am Soc Mass Spectrom 10:770\u2013781. https:\/\/doi.org\/10.1016\/S1044-0305(99)00047-1","journal-title":"J Am Soc Mass Spectrom"},{"key":"12650_CR58","doi-asserted-by":"publisher","first-page":"721","DOI":"10.1007\/s00253-009-2182-6","volume":"85","author":"ML Thompson","year":"2010","unstructured":"Thompson ML, Marriott R, Dowle A, Grogan G (2010) Biotransformation of \u03b2-myrcene to geraniol by a strain of Rhodococcus erythropolis isolated by selective enrichment from hop plants. Appl Microbiol Biotechnol 85:721\u2013730. https:\/\/doi.org\/10.1007\/s00253-009-2182-6","journal-title":"Appl Microbiol Biotechnol"},{"key":"12650_CR59","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1093\/genetics\/144.1.15","volume":"144","author":"J Torreblanca","year":"1996","unstructured":"Torreblanca J, Casades\u00fas J (1996) DNA adenine methylase mutants of Salmonella typhimurium and a novel Dam-regulated locus. Genetics 144:15\u201326. https:\/\/doi.org\/10.1093\/genetics\/144.1.15","journal-title":"Genetics"},{"key":"12650_CR60","doi-asserted-by":"publisher","first-page":"4837","DOI":"10.1128\/AEM.69.8.4837-4845.2003","volume":"69","author":"A Toussaint","year":"2003","unstructured":"Toussaint A, Merlin C, Monchy S, Benotmane MA, Leplae R, Mergeay M, Springael D (2003) The biphenyl-and 4-chlorobiphenyl-catabolic transposon Tn4371, a member of a new family of genomic islands related to IncP and Ti plasmids. Appl Environ Microbiol 69:4837\u20134845. https:\/\/doi.org\/10.1128\/AEM.69.8.4837-4845.2003","journal-title":"Appl Environ Microbiol"},{"key":"12650_CR61","doi-asserted-by":"publisher","first-page":"2061","DOI":"10.1128\/jb.176.7.2061-2067.1994","volume":"176","author":"JE Trempy","year":"1994","unstructured":"Trempy JE, Kirby JE, Gottesman S (1994) Alp suppression of Lon: dependence on the slpA gene. J Bacteriol 176:2061\u20132067. https:\/\/doi.org\/10.1128\/jb.176.7.2061-2067.1994","journal-title":"J Bacteriol"},{"issue":"W1","key":"12650_CR62","doi-asserted-by":"publisher","first-page":"W232","DOI":"10.1093\/nar\/gkw256","volume":"44","author":"J Trifinopoulos","year":"2016","unstructured":"Trifinopoulos J, Nguyen L-T, von Haeseler A, Minh BQ (2016) W-IQ-TREE: a fast online phylogenetic tool for maximum likelihood analysis. Nucleic Acids Res 44(W1):W232\u2013W235. https:\/\/doi.org\/10.1093\/nar\/gkw256","journal-title":"Nucleic Acids Res"},{"key":"12650_CR63","doi-asserted-by":"publisher","first-page":"463","DOI":"10.1016\/S0021-9673(01)80947-X","volume":"11","author":"H van Den Dool","year":"1963","unstructured":"van Den Dool H, Kratz P (1963) A generalization of the retention index system including linear temperature programmed gas\u2014liquid partition chromatography. J Chromatogr A 11:463\u2013471. https:\/\/doi.org\/10.1016\/S0021-9673(01)80947-X","journal-title":"J Chromatogr A"},{"key":"12650_CR64","doi-asserted-by":"publisher","first-page":"1129","DOI":"10.1099\/00221287-146-5-1129","volume":"146","author":"MJ van der Werf","year":"2000","unstructured":"van der Werf MJ, Boot AM (2000) Metabolism of carveol and dihydrocarveol in Rhodococcus erythropolis DCL14. Microbiology 146:1129\u20131141. https:\/\/doi.org\/10.1099\/00221287-146-5-1129","journal-title":"Microbiology"},{"key":"12650_CR65","doi-asserted-by":"publisher","first-page":"2092","DOI":"10.1128\/AEM.65.5.2092-2102.1999","volume":"65","author":"MJ van der Werf","year":"1999","unstructured":"van der Werf MJ, Swarts HJ, de Bont JAM (1999) Rhodococcus erythropolis DCL14 contains a novel degradation pathway for limonene. Appl Environ Microbiol 65:2092\u20132102. https:\/\/doi.org\/10.1128\/AEM.65.5.2092-2102.1999","journal-title":"Appl Environ Microbiol"},{"key":"12650_CR66","doi-asserted-by":"publisher","first-page":"e100740","DOI":"10.1371\/journal.pone.0100740","volume":"9","author":"C Vilanova","year":"2014","unstructured":"Vilanova C, Mar\u00edn M, Baixeras J, Latorre A, Porcar M (2014) Selecting microbial strains from pine tree resin: biotechnological applications from a terpene world. PLoS One 9:e100740. https:\/\/doi.org\/10.1371\/journal.pone.0100740","journal-title":"PLoS One"},{"key":"12650_CR67","doi-asserted-by":"publisher","first-page":"755","DOI":"10.1023\/A:1015236709767","volume":"28","author":"D Vokou","year":"2002","unstructured":"Vokou D, Chalkos D, Karamanlidou G, Yiangou M (2002) Activation of soil respiration and shift of the microbial population balance in soil as a response to Lavandula stoechas essential oil. J Chem Ecol 28:755\u2013768. https:\/\/doi.org\/10.1023\/A:1015236709767","journal-title":"J Chem Ecol"},{"key":"12650_CR68","doi-asserted-by":"publisher","unstructured":"Wallace WE (2023). Retention indices in NIST Chemistry WebBook. In: Linstrom PJ, Mallard WG (eds) NIST standard reference database number 69. National Institute of Standards and Technology, Gaithersburg MD, p 20899.\u00a0 https:\/\/doi.org\/10.18434\/T4D303","DOI":"10.18434\/T4D303"},{"key":"12650_CR69","doi-asserted-by":"publisher","first-page":"e1005595","DOI":"10.1371\/journal.pcbi.1005595","volume":"13","author":"RR Wick","year":"2017","unstructured":"Wick RR, Judd LM, Gorrie CL, Holt KE (2017) Unicycler: resolving bacterial genome assemblies from short and long sequencing reads. PLoS Comput Biol 13:e1005595. https:\/\/doi.org\/10.1371\/journal.pcbi.1005595","journal-title":"PLoS Comput Biol"},{"key":"12650_CR70","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1007\/BF00993688","volume":"19","author":"FM Wilt","year":"1993","unstructured":"Wilt FM, Miller GC, Everett RL, Hackett M (1993) Monoterpene concentrations in fresh, senescent, and decaying foliage of singleleaf pinyon (Pinus monophylla Torr. & Frem.: Pinaceae) from the western Great Basin. J Chem Ecol 19:185\u2013194. https:\/\/doi.org\/10.1007\/BF00993688","journal-title":"J Chem Ecol"},{"key":"12650_CR71","doi-asserted-by":"publisher","first-page":"183","DOI":"10.1038\/nrmicro1350","volume":"4","author":"D Wion","year":"2006","unstructured":"Wion D, Casades\u00fas J (2006) N6-methyl-adenine: an epigenetic signal for DNA\u2013protein interactions. Nat Rev Microbiol 4:183. https:\/\/doi.org\/10.1038\/nrmicro1350","journal-title":"Nat Rev Microbiol"},{"key":"12650_CR72","doi-asserted-by":"publisher","first-page":"552","DOI":"10.1038\/nrmicro2382","volume":"8","author":"RAF Wozniak","year":"2010","unstructured":"Wozniak RAF, Waldor MK (2010) Integrative and conjugative elements: mosaic mobile genetic elements enabling dynamic lateral gene flow. Nat Rev Microbiol 8:552\u2013563. https:\/\/doi.org\/10.1038\/nrmicro2382","journal-title":"Nat Rev Microbiol"}],"container-title":["Applied Microbiology and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-023-12650-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00253-023-12650-w\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-023-12650-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,29]],"date-time":"2023-07-29T22:02:54Z","timestamp":1690668174000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00253-023-12650-w"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,5]]},"references-count":72,"journal-issue":{"issue":"16","published-print":{"date-parts":[[2023,8]]}},"alternative-id":["12650"],"URL":"https:\/\/doi.org\/10.1007\/s00253-023-12650-w","relation":{},"ISSN":["0175-7598","1432-0614"],"issn-type":[{"value":"0175-7598","type":"print"},{"value":"1432-0614","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7,5]]},"assertion":[{"value":"21 March 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 June 2023","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 June 2023","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"5 July 2023","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This article does not contain any studies with human participants or animals performed by any of the authors.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}