{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T11:51:14Z","timestamp":1776081074163,"version":"3.50.1"},"reference-count":43,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,2,2]],"date-time":"2024-02-02T00:00:00Z","timestamp":1706832000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,2,2]],"date-time":"2024-02-02T00:00:00Z","timestamp":1706832000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/04469\/2020"],"award-info":[{"award-number":["UIDB\/04469\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BD\/132845\/2017, COVID\/BD\/152450\/2022"],"award-info":[{"award-number":["SFRH\/BD\/132845\/2017, COVID\/BD\/152450\/2022"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Programa Operacional Regional do Norte\u2014BioEcoNorte project","award":["NORTE-01-0145-FEDER-000070"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-000070"]}]},{"DOI":"10.13039\/501100008814","name":"Universidade do Minho","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100008814","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Appl Microbiol Biotechnol"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:sec>\n            <jats:title>Abstract<\/jats:title>\n            <jats:p>Micro-aeration was shown to improve anaerobic digestion (AD) processes, although oxygen is known to inhibit obligate anaerobes, such as syntrophic communities of bacteria and methanogens. The effect of micro-aeration on the activity and microbial interaction in syntrophic communities, as well as on the potential establishment of synergetic relationships with facultative anaerobic bacteria (FAB) or aerobic bacteria (AB), was investigated. Anaerobic sludge was incubated with ethanol and increasing oxygen concentrations (0\u20135% in the headspace). Assays with acetate or H<jats:sub>2<\/jats:sub>\/CO<jats:sub>2<\/jats:sub> (direct substrates for methanogens) were also performed. When compared with the controls (0% O<jats:sub>2<\/jats:sub>), oxygen significantly decreased substrate consumption and initial methane production rate (MPR) from acetate or H<jats:sub>2<\/jats:sub>\/CO<jats:sub>2<\/jats:sub>. At 0.5% O<jats:sub>2<\/jats:sub>, MPR from these substrates was inhibited 30\u201340%, and close to 100% at 5% O<jats:sub>2<\/jats:sub>. With ethanol, significant inhibition (&gt;36%) was only observed for oxygen concentrations higher than 2.5%. Oxygen was consumed in the assays, pointing to the stimulation of AB\/FAB by ethanol, which helped to protect the syntrophic consortia under micro-aerobic conditions. This highlights the importance of AB\/FAB in maintaining functional and resilient syntrophic communities, which is relevant for real AD systems (in which vestigial O<jats:sub>2<\/jats:sub> amounts are frequently present), as well as for AD systems using micro-aeration as a process strategy.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Key points<\/jats:title>\n            <jats:p>\u2022<jats:italic>Micro-aeration impacts syntrophic communities of bacteria and methanogens.<\/jats:italic>\n            <\/jats:p>\n            <jats:p>\u2022<jats:italic>Oxygen stimulates AB\/FAB, maintaining functional and resilient consortia.<\/jats:italic>\n            <\/jats:p>\n            <jats:p>\u2022<jats:italic>Micro-aeration studies are critical for systems using micro-aeration as a process strategy.<\/jats:italic>\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1007\/s00253-023-12969-4","type":"journal-article","created":{"date-parts":[[2024,2,2]],"date-time":"2024-02-02T09:02:30Z","timestamp":1706864550000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Effect of micro-aeration on syntrophic and methanogenic activity in anaerobic sludge"],"prefix":"10.1007","volume":"108","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1139-0713","authenticated-orcid":false,"given":"Bruno P.","family":"Morais","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4763-4989","authenticated-orcid":false,"given":"Carla P.","family":"Magalh\u00e3es","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7187-0538","authenticated-orcid":false,"given":"Gilberto","family":"Martins","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7110-1779","authenticated-orcid":false,"given":"Maria Alcina","family":"Pereira","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7999-4620","authenticated-orcid":false,"given":"Ana J.","family":"Cavaleiro","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2024,2,2]]},"reference":[{"key":"12969_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41467-017-01753-4","volume":"8","author":"JC Angle","year":"2017","unstructured":"Angle JC, Morin TH, Solden LM, Narrowe AB, Smith GJ, Borton MA, Rey-Sanchez C, Daly AR, Mirfenderesgi G, Hoyt DW, Riley WJ, Miller CS, Bohrer G, Wrighton KC (2017) Methanogenesis in oxygenated soils is a substantial fraction of wetland methane emissions. Nat Commun 8:1\u20139. https:\/\/doi.org\/10.1038\/s41467-017-01753-4","journal-title":"Nat Commun"},{"key":"12969_CR2","doi-asserted-by":"publisher","first-page":"1","DOI":"10.2174\/1876400201104010001","volume":"4","author":"D Botheju","year":"2011","unstructured":"Botheju D, Bakke R (2011) Oxygen effects in anaerobic digestion \u2013 a review. Open Waste Manag J 4:1\u201319. https:\/\/doi.org\/10.2174\/1876400201104010001","journal-title":"Open Waste Manag J"},{"key":"12969_CR3","doi-asserted-by":"publisher","first-page":"274","DOI":"10.1016\/j.mib.2011.04.015","volume":"14","author":"C Brochier-Armanet","year":"2011","unstructured":"Brochier-Armanet C, Forterre P, Gribaldo S (2011) Phylogeny and evolution of the Archaea: one hundred genomes later. Curr Opin Microbiol 14:274\u2013281. https:\/\/doi.org\/10.1016\/j.mib.2011.04.015","journal-title":"Curr Opin Microbiol"},{"key":"12969_CR4","doi-asserted-by":"publisher","first-page":"3082","DOI":"10.1021\/acs.est.5b03204","volume":"50","author":"AJ Cavaleiro","year":"2016","unstructured":"Cavaleiro AJ, Pereira MA, Guedes AP, Stams AJM, Alves MM, Sousa DZ (2016) Conversion of Cn-unsaturated into Cn-2-saturated LCFA can occur uncoupled from methanogenesis in anaerobic bioreactors. Environ Sci Technol 50:3082\u20133090. https:\/\/doi.org\/10.1021\/acs.est.5b03204","journal-title":"Environ Sci Technol"},{"key":"12969_CR5","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1016\/S0168-1605(01)00715-2","volume":"74","author":"WJ du Toit","year":"2002","unstructured":"du Toit WJ, Lambrechts MG (2002) The enumeration and identification of acetic acid bacteria from South African red wine fermentations. Int J Food Microbiol 74:57\u201364. https:\/\/doi.org\/10.1016\/S0168-1605(01)00715-2","journal-title":"Int J Food Microbiol"},{"key":"12969_CR6","first-page":"76","volume":"27","author":"WJ du Toit","year":"2006","unstructured":"du Toit WJ, Marais J, Pretorius IS, du Toit M (2006) Oxygen in must and wine: a review. S Afr J Enol Vitic 27:76\u201394","journal-title":"S Afr J Enol Vitic"},{"key":"12969_CR7","doi-asserted-by":"publisher","first-page":"3650","DOI":"10.1111\/1462-2920.15124","volume":"22","author":"MS Duarte","year":"2020","unstructured":"Duarte MS, Salvador AF, Cavaleiro AJ, Stams AJM, Pereira MA, Alves MM (2020) Multiple and flexible roles of facultative anaerobic bacteria in microaerophilic oleate degradation. Environ Microbiol 22:3650\u20133659. https:\/\/doi.org\/10.1111\/1462-2920.15124","journal-title":"Environ Microbiol"},{"key":"12969_CR8","unstructured":"European Biogas Association (2022) About Biogas and Biomethane. https:\/\/www.europeanbiogas.eu\/about-biogas-and-biomethane\/. Accessed 29 Mar 2022"},{"key":"12969_CR9","unstructured":"European Commission (2022) REPowerEU: affordable, secure and sustainable energy for Europe. https:\/\/commission.europa.eu\/strategy-and-policy\/priorities-2019-2024\/european-green-deal\/repowereu-affordable-secure-and-sustainable-energy-europe_en. Accessed 20 Dec 2022"},{"key":"12969_CR10","doi-asserted-by":"publisher","first-page":"108","DOI":"10.1007\/BF00288711","volume":"160","author":"S Fetzer","year":"1993","unstructured":"Fetzer S, Conrad R (1993) Effect of redox potential on methanogenesis by Methanosarcina barkeri. Arch Microbiol 160:108\u2013113. https:\/\/doi.org\/10.1007\/BF00288711","journal-title":"Arch Microbiol"},{"key":"12969_CR11","doi-asserted-by":"publisher","first-page":"714","DOI":"10.1016\/j.tibtech.2022.09.008","volume":"41","author":"S Fu","year":"2023","unstructured":"Fu S, Lian S, Angelidaki I, Guo R (2023) Micro-aeration: an attractive strategy to facilitate anaerobic digestion. Trends Biotechnol 41:714\u2013726. https:\/\/doi.org\/10.1016\/j.tibtech.2022.09.008","journal-title":"Trends Biotechnol"},{"key":"12969_CR12","doi-asserted-by":"publisher","first-page":"1571","DOI":"10.1016\/j.procbio.2014.07.003","volume":"49","author":"M Gullo","year":"2014","unstructured":"Gullo M, Verzelloni E, Canonico M (2014) Aerobic submerged fermentation by acetic acid bacteria for vinegar production: process and biotechnological aspects. Process Biochem 49:1571\u20131579. https:\/\/doi.org\/10.1016\/j.procbio.2014.07.003","journal-title":"Process Biochem"},{"key":"12969_CR13","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1371\/journal.pone.0117331","volume":"10","author":"R Jasso-Ch\u00e1vez","year":"2015","unstructured":"Jasso-Ch\u00e1vez R, Santiago-Mart\u00ednez MG, Lira-Silva E, Pineda E, Zepeda-Rodr\u00edguez A, Belmont-D\u00edaz J, Encalada R, Saavedra E, Moreno-S\u00e1nchez R (2015) Air-adapted Methanosarcina acetivorans shows high methane production and develops resistance against oxygen stress. PLoS One 10:1\u201321. https:\/\/doi.org\/10.1371\/journal.pone.0117331","journal-title":"PLoS One"},{"key":"12969_CR14","doi-asserted-by":"publisher","first-page":"428","DOI":"10.1016\/j.tim.2020.10.001","volume":"29","author":"M Khademian","year":"2021","unstructured":"Khademian M, Imlay JA (2021) How microbes evolved to tolerate oxygen. Trends Microbiol 29:428\u2013440. https:\/\/doi.org\/10.1016\/j.tim.2020.10.001","journal-title":"Trends Microbiol"},{"key":"12969_CR15","doi-asserted-by":"publisher","first-page":"305","DOI":"10.1016\/S0723-2020(83)80017-4","volume":"4","author":"A Kiener","year":"1983","unstructured":"Kiener A, Leisinger T (1983) Oxygen sensitivity of methanogenic bacteria. Syst Appl Microbiol 4:305\u2013312. https:\/\/doi.org\/10.1016\/S0723-2020(83)80017-4","journal-title":"Syst Appl Microbiol"},{"key":"12969_CR16","doi-asserted-by":"publisher","first-page":"156454","DOI":"10.1016\/j.scitotenv.2022.156454","volume":"838","author":"J Li","year":"2022","unstructured":"Li J, Ran X, Zhou M, Wang K, Wang H, Wang Y (2022) Oxidative stress and antioxidant mechanisms of obligate anaerobes involved in biological waste treatment processes: a review. Sci Total Environ 838:156454. https:\/\/doi.org\/10.1016\/j.scitotenv.2022.156454","journal-title":"Sci Total Environ"},{"key":"12969_CR17","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1016\/j.coal.2013.02.010","volume":"115","author":"Y Liu","year":"2013","unstructured":"Liu Y, Urynowicz MA, Bagley DM (2013) Ethanol conversion to methane by a coal microbial community. Int J Coal Geol 115:85\u201391. https:\/\/doi.org\/10.1016\/j.coal.2013.02.010","journal-title":"Int J Coal Geol"},{"key":"12969_CR18","doi-asserted-by":"publisher","first-page":"774","DOI":"10.1038\/s41579-021-00583-y","volume":"19","author":"Z Lu","year":"2021","unstructured":"Lu Z, Imlay JA (2021) When anaerobes encounter oxygen: mechanisms of oxygen toxicity, tolerance and defence. Nat Rev Microbiol 19:774\u2013785. https:\/\/doi.org\/10.1038\/s41579-021-00583-y","journal-title":"Nat Rev Microbiol"},{"key":"12969_CR19","doi-asserted-by":"publisher","first-page":"411","DOI":"10.1038\/ismej.2017.173","volume":"12","author":"Z Lyu","year":"2018","unstructured":"Lyu Z, Lu Y (2018) Metabolic shift at the class level sheds light on adaptation of methanogens to oxidative environments. ISME J 12:411\u2013423. https:\/\/doi.org\/10.1038\/ismej.2017.173","journal-title":"ISME J"},{"key":"12969_CR20","doi-asserted-by":"publisher","first-page":"377","DOI":"10.1007\/s12088-013-0414-z","volume":"53","author":"D Mamlouk","year":"2013","unstructured":"Mamlouk D, Gullo M (2013) Acetic acid bacteria: physiology and carbon sources oxidation. Indian J Microbiol 53:377\u2013384. https:\/\/doi.org\/10.1007\/s12088-013-0414-z","journal-title":"Indian J Microbiol"},{"key":"12969_CR21","doi-asserted-by":"publisher","first-page":"623","DOI":"10.1016\/j.copbio.2009.10.001","volume":"20","author":"MJ McInerney","year":"2009","unstructured":"McInerney MJ, Sieber JR, Gunsalus RP (2009) Syntrophy in anaerobic global carbon cycles. Curr Opin Biotechnol 20:623\u2013632. https:\/\/doi.org\/10.1016\/j.copbio.2009.10.001","journal-title":"Curr Opin Biotechnol"},{"key":"12969_CR22","doi-asserted-by":"publisher","first-page":"8191","DOI":"10.1128\/AEM.71.12.8191-8200.2005","volume":"71","author":"M Metje","year":"2005","unstructured":"Metje M, Frenzel P (2005) Effect of temperature on anaerobic ethanol oxidation and methanogenesis in acidic peat from a Northern Wetland. Appl Environ Microbiol 71:8191\u20138200. https:\/\/doi.org\/10.1128\/AEM.71.12.8191-8200.2005","journal-title":"Appl Environ Microbiol"},{"key":"12969_CR23","doi-asserted-by":"publisher","first-page":"1971","DOI":"10.1016\/j.biotechadv.2018.08.007","volume":"36","author":"D Nguyen","year":"2018","unstructured":"Nguyen D, Khanal SK (2018) A little breath of fresh air into an anaerobic system: how microaeration facilitates anaerobic digestion process. Biotechnol Adv 36:1971\u20131983. https:\/\/doi.org\/10.1016\/j.biotechadv.2018.08.007","journal-title":"Biotechnol Adv"},{"key":"12969_CR24","doi-asserted-by":"publisher","first-page":"763","DOI":"10.1016\/B978-0-12-816856-1.00031-2","volume-title":"Biofuels: Alternative Feedstocks and Conversion Processes for the Production of Liquid and Gaseous biofuels","author":"D Nguyen","year":"2019","unstructured":"Nguyen D, Nitayavardhana S, Sawatdeenarunat C, Surendra KC, Khanal SK (2019a) Biogas production by anaerobic digestion: status and perspectives. In: Biofuels: Alternative Feedstocks and Conversion Processes for the Production of Liquid and Gaseous biofuels, 2nd edn. Elsevier Inc., pp 763\u2013778","edition":"2"},{"key":"12969_CR25","doi-asserted-by":"publisher","first-page":"115080","DOI":"10.1016\/j.watres.2019.115080","volume":"166","author":"D Nguyen","year":"2019","unstructured":"Nguyen D, Wu Z, Shrestha S, Lee PH, Raskin L, Khanal SK (2019b) Intermittent micro-aeration: new strategy to control volatile fatty acid accumulation in high organic loading anaerobic digestion. Water Res 166:115080. https:\/\/doi.org\/10.1016\/j.watres.2019.115080","journal-title":"Water Res"},{"key":"12969_CR26","doi-asserted-by":"publisher","first-page":"228","DOI":"10.1139\/m84-034","volume":"30","author":"GB Patel","year":"1984","unstructured":"Patel GB, Roth LA, Agnew BJ (1984) Death rates of obligate anaerobes exposed to oxygen and the effect of media prereduction on cell viability. Can J Microbiol 30:228\u2013235","journal-title":"Can J Microbiol"},{"key":"12969_CR27","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1002\/bit.20548","volume":"92","author":"MA Pereira","year":"2005","unstructured":"Pereira MA, Pires OC, Mota M, Alves MM (2005) Anaerobic biodegradation of oleic and palmitic acids: evidence of mass transfer limitations caused by long chain fatty acid accumulation onto the anaerobic sludge. Biotechnol Bioeng 92:15\u201323. https:\/\/doi.org\/10.1002\/bit.20548","journal-title":"Biotechnol Bioeng"},{"key":"12969_CR28","doi-asserted-by":"publisher","first-page":"317","DOI":"10.1271\/bbb.61.317","volume":"61","author":"A Saeki","year":"1997","unstructured":"Saeki A, Taniguchi M, Matsushita K, Toyama H, Theeragool G, Lotong N, Adachi O (1997) Microbiological aspects of acetate oxidation by acetic acid bacteria, unfavorable phenomena in vinegar fermentation. Biosci Biotechnol Biochem 61:317\u2013323. https:\/\/doi.org\/10.1271\/bbb.61.317","journal-title":"Biosci Biotechnol Biochem"},{"key":"12969_CR29","doi-asserted-by":"publisher","first-page":"1260","DOI":"10.1016\/j.biotechadv.2014.12.001","volume":"33","author":"N Saichana","year":"2015","unstructured":"Saichana N, Matsushita K, Adachi O, Fr\u00e9bort I, Frebortova J (2015) Acetic acid bacteria: a group of bacteria with versatile biotechnological applications. Biotechnol Adv 33:1260\u20131271","journal-title":"Biotechnol Adv"},{"key":"12969_CR30","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1016\/j.jbiosc.2011.11.005","volume":"113","author":"K Sakurai","year":"2012","unstructured":"Sakurai K, Arai H, Ishii M, Igarashi Y (2012) Changes in the gene expression profile of Acetobacter aceti during growth on ethanol. J Biosci Bioeng 113:343\u2013348. https:\/\/doi.org\/10.1016\/j.jbiosc.2011.11.005","journal-title":"J Biosci Bioeng"},{"key":"12969_CR31","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1016\/0378-1097(85)90002-3","volume":"31","author":"B Schink","year":"1985","unstructured":"Schink B (1985) Degradation of unsaturated hydrocarbons by methanogenic enrichment cultures. FEMS Microbiol Lett 31:69\u201377. https:\/\/doi.org\/10.1016\/0378-1097(85)90002-3","journal-title":"FEMS Microbiol Lett"},{"key":"12969_CR32","doi-asserted-by":"publisher","first-page":"429","DOI":"10.1146\/annurev-micro-090110-102844","volume":"66","author":"JR Sieber","year":"2012","unstructured":"Sieber JR, McInerney MJ, Gunsalus RP (2012) Genomic insights into syntrophy: the paradigm for anaerobic metabolic cooperation. Annu Rev Microbiol 66:429\u2013452. https:\/\/doi.org\/10.1146\/annurev-micro-090110-102844","journal-title":"Annu Rev Microbiol"},{"key":"12969_CR33","doi-asserted-by":"publisher","first-page":"514","DOI":"10.1111\/1751-7915.12365","volume":"9","author":"SA Silva","year":"2016","unstructured":"Silva SA, Salvador AF, Cavaleiro AJ, Pereira MA, Stams AJM, Alves MM, Sousa DZ (2016) Toxicity of long chain fatty acids towards acetate conversion by Methanosaeta concilii and Methanosarcina mazei. Microb Biotechnol 9:514\u2013518. https:\/\/doi.org\/10.1111\/1751-7915.12365","journal-title":"Microb Biotechnol"},{"key":"12969_CR34","doi-asserted-by":"publisher","first-page":"568","DOI":"10.1038\/nrmicro2166","volume":"7","author":"AJM Stams","year":"2009","unstructured":"Stams AJM, Plugge CM (2009) Electron transfer in syntrophic communities of anaerobic bacteria and archaea. Nat Rev Microbiol 7:568\u2013577. https:\/\/doi.org\/10.1038\/nrmicro2166","journal-title":"Nat Rev Microbiol"},{"key":"12969_CR35","doi-asserted-by":"publisher","first-page":"1114","DOI":"10.1128\/aem.59.4.1114-1119.1993","volume":"59","author":"AJM Stams","year":"1993","unstructured":"Stams AJM, Van Dijk JB, Dijkema C, Plugge CM (1993) Growth of syntrophic propionate-oxidizing bacteria with fumarate in the absence of methanogenic bacteria. Appl Environ Microbiol 59:1114\u20131119","journal-title":"Appl Environ Microbiol"},{"key":"12969_CR36","doi-asserted-by":"publisher","first-page":"100","DOI":"10.1128\/br.41.1.100-180.1977","volume":"41","author":"RK Thauer","year":"1977","unstructured":"Thauer RK, Jungermann K, Decker K (1977) Energy conservation in chemotrophic anaerobic bacteria. Bacteriol Rev 41:100\u2013180","journal-title":"Bacteriol Rev"},{"key":"12969_CR37","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1128\/aem.54.1.20-29.1988","volume":"54","author":"JH Thiele","year":"1988","unstructured":"Thiele JH, Zeikus JG (1988) Control of interspecies electron flow during anaerobic digestion: significance of formate transfer versus hydrogen transfer during syntrophic methanogenesis in flocs. Appl Environ Microbiol 54:20\u201329","journal-title":"Appl Environ Microbiol"},{"key":"12969_CR38","doi-asserted-by":"publisher","first-page":"246","DOI":"10.1016\/j.biortech.2016.11.081","volume":"225","author":"P Tsapekos","year":"2017","unstructured":"Tsapekos P, Kougias PG, Vasileiou SA, Lyberatos G, Angelidaki I (2017) Effect of micro-aeration and inoculum type on the biodegradation of lignocellulosic substrate. Bioresour Technol 225:246\u2013253. https:\/\/doi.org\/10.1016\/j.biortech.2016.11.081","journal-title":"Bioresour Technol"},{"key":"12969_CR39","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1007\/s11274-015-1799-0","volume":"31","author":"B Wang","year":"2015","unstructured":"Wang B, Shao Y, Chen F (2015) Overview on mechanisms of acetic acid resistance in acetic acid bacteria. World J Microbiol Biotechnol 31:255\u2013263. https:\/\/doi.org\/10.1007\/s11274-015-1799-0","journal-title":"World J Microbiol Biotechnol"},{"key":"12969_CR40","doi-asserted-by":"publisher","first-page":"3438","DOI":"10.1128\/aem.57.12.3438-3449.1991","volume":"57","author":"WM Wu","year":"1991","unstructured":"Wu WM, Hickey RF, Zeikus JG (1991) Characterization of metabolic performance of methanogenic granules treating brewery wastewater: role of sulfate-reducing bacteria. Appl Environ Microbiol 57:3438\u20133449. https:\/\/doi.org\/10.1128\/aem.57.12.3438-3449.1991","journal-title":"Appl Environ Microbiol"},{"key":"12969_CR41","doi-asserted-by":"publisher","first-page":"363","DOI":"10.1016\/j.wasman.2013.10.038","volume":"34","author":"S Xu","year":"2014","unstructured":"Xu S, Selvam A, Wong JWC (2014) Optimization of micro-aeration intensity in acidogenic reactor of a two-phase anaerobic digester treating food waste. Waste Manag 34:363\u2013369. https:\/\/doi.org\/10.1016\/j.wasman.2013.10.038","journal-title":"Waste Manag"},{"key":"12969_CR42","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1016\/j.ijfoodmicro.2007.11.077","volume":"125","author":"Y Yamada","year":"2008","unstructured":"Yamada Y, Yukphan P (2008) Genera and species in acetic acid bacteria. Int J Food Microbiol 125:15\u201324. https:\/\/doi.org\/10.1016\/j.ijfoodmicro.2007.11.077","journal-title":"Int J Food Microbiol"},{"key":"12969_CR43","doi-asserted-by":"publisher","first-page":"426","DOI":"10.1016\/j.apenergy.2014.12.069","volume":"142","author":"NHM Yasin","year":"2015","unstructured":"Yasin NHM, Maeda T, Hu A, Yu C-P, Wood TK (2015) CO2 sequestration by methanogens in activated sludge for methane production. Appl Energy 142:426\u2013434. https:\/\/doi.org\/10.1016\/j.apenergy.2014.12.069","journal-title":"Appl Energy"}],"container-title":["Applied Microbiology and Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-023-12969-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00253-023-12969-4\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00253-023-12969-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T20:46:20Z","timestamp":1748378780000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00253-023-12969-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,2]]},"references-count":43,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,12]]}},"alternative-id":["12969"],"URL":"https:\/\/doi.org\/10.1007\/s00253-023-12969-4","relation":{},"ISSN":["0175-7598","1432-0614"],"issn-type":[{"value":"0175-7598","type":"print"},{"value":"1432-0614","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2,2]]},"assertion":[{"value":"12 September 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 November 2023","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"28 November 2023","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 February 2024","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This article does not contain any studies with human participants or animals performed by any of the authors.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}],"article-number":"192"}}